Page 1
Operating Manual Refrigerated Floor Standing Centrifuge 8K(B) Please retain for later use! Version 01/2009, Rev. 1.17 of 27/01/2012 Translation of the original operating manual...
Page 3
8K(B) In case of inquiries please state the following number: Serial number: Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Phone +49 (0) 55 22 / 50 07-0 +49 (0) 55 22 / 50 07-12 E-mail: info@sigma-zentrifugen.de...
8K(B) Table of Contents Table of Contents General Information Importance of the Operating Manual ................9 Intended Use......................9 Warranty and Liability ....................9 Copyright........................10 Standards and Regulations ..................10 Scope of Supply ....................... 10 Design of the Centrifuge Overview ........................
Page 6
8K(B) Table of Contents Storage and Transport Dimensions and Weight.................... 21 Storage Conditions ....................21 Notes on Transport....................21 Set-up and Connection Unpacking the Centrifuge ..................23 Installation Site ......................23 Set-up and Alignment of the Centrifuge ..............23 5.3.1 Set-up ......................
Page 7
8K(B) Table of Contents 6.3.4 Program Mode .................... 47 6.3.4.1 Loading a Program ..............47 6.3.4.2 Saving a Program ............... 48 6.3.4.3 Deleting a Program ..............48 6.3.4.4 Automatic Program Rotation ............49 6.3.4.5 Options for Data Input and Output (can be retrofitted)....49 Switching the Centrifuge OFF...................
Page 8
8K(B) Table of Contents 11.4 Formulae - Mathematical Relations ................74 11.4.1 Relative Centrifugal Force (RCF) ..............74 11.4.2 Density......................74 11.4.3 Speed-Gravitational-Field-Diagram............. 74 Table of rotors and accessories with a different service life......76 Resistance Data..................77 EC-Declaration of Conformity..............81 Layout Plan....................
They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. SIGMA Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use. The intended use also includes...
8K(B) General Information Copyright The copyright concerning the operating manual remains Sigma Laborzentrifugen GmbH. The operatin manual is solely intended for the operator and their personnel. It includes instructions and information that may not be duplicated, distributet, or communicated in any othe way neither in full nor in parts.
Castor Fig. 2.2: Rear view of an air cooled centrifuge Cooling water connection (see 5.6) Fig. 2.3: Rear view of a water cooled centrifuge Not for Sigma 8KB Version 01/2009, Rev. 1.17 of 27/01/2012 Translation of the original operating manual...
8K(B) Design of the Centrifuge Name Plate Manufacturer and registered office Type name Serial number Max. speed Max. kinetic energy Max. density Nominal voltage Input fuse Symbol for special disposal (see chapter 9) CE mark in accordance with the directive 94/9/EC...
8K(B) Safety Safety Marking of the Unit International symbols used for SIGMA centrifuges: Gefährliche elektrische Spannung Dangerous voltage Courant haute tension Achtung, Betriebsanleitung lesen Attention, consult instruction manual Attention, consulter mode d'emploi Ein (Netzverbindung) On (Power) Marche (mise sous tension)
8K(B) Safety Explanation of the Symbols and Notes This operating manual uses the following names and symbols to indicate hazards: This symbol stands for a direct hazard to the life and health of persons. Non-observance of these symbols causes serious health problems up to life-endangering injuries.
8K(B) Safety Responsibility of the Operator The operator is responsible to authorise only qualified personnel to work on the machine (see chapter 3.4 "Operating Personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.
Stop the centrifuge immediately in the event of a malfunction (see chapter 7 "Malfunctions and Error Correction") or contact the service of SIGMA Laborzentrifugen GmbH (see 7.3 "Service Contact"). Ensure that all repairs are performed only by authorized and specialized personnel (see 7.3 "Service Contact").
8K(B) Safety Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, adapter) in terms of visible structural changes.
8K(B) Safety Only use the centrifuge with rotors and accessories that have been approved by the manufacturer. We explicitly warn against the use of equipment of poor quality. Breaking glass or bursting vessels can cause dangerous imbalances at high speeds.
8K(B) Safety 3.6.7 Resistance of Plastics Chemical influences have a strong effect on the polymeric chains of plastics, and therefore, on their physical properties. Plastic parts can be damaged if solvents, acids, or alkaline solutions are used. Refer to the resistance data (see chapter 11 "Appendix")! Safety Devices 3.7.1...
8K(B) Safety 3.7.7 Rotor Monitoring After the selection of a rotor number and also after the selection of a bucket number (if applicable), the system checks automatically if the set speed or the set gravitational field is permissible for the selected rotor.
8K(B) Storage and Transport Storage and Transport Dimensions and Weight Height: 980 mm Height with opened lid: 1 690 mm Width: 810 mm Depth: 910 mm Weight: 420 kg Fig. 4.1: Dimensions and weight Storage Conditions The centrifuge can be stored for up to a year without any problems.
Air cooled centrifuges: Ensure sufficient ventilation. The centrifuge 8K (B) with an air-cooled compressor should not be set up with its left side against a wall, since otherwise the hot air, which is emitted out the back, will be drawn in again as fresh air for cooling. As a result, the unit will switch off due to overheating (see also 7.2 "Error...
8K(B) Set-up and Connection 5.3.1 Set-up Transport the centrifuge as closely as possible to the installation site with a lifting device (e.g. forklift). Set the centrifuge down. To place the centrifuge on the castors, open the front door with the supplied square spanner (part no. 930 114) by turning it clockwise by 90°.
8K(B) Set-up and Connection 5.3.2 Alignment For reasons of safety, the centrifuge must not be operated while it is set up on its castors! The centrifuge must be set-up stably and horizontally at the installation site. Set the centrifuge on its adjustable feet (see 5.3.1 in reverse order) To do so, turn all of the feet clockwise by hand until they touch the ground.
SIGMA centrifuges of the 8K (B) series are units of safety class I and have a five-wire power cord (2.5 m) with a 5-channel Cekon-plug (16 A). Behind the front door there are three fuses with a rocker switch.
8K(B) Set-up and Connection Drain outside of the centrifuge Plug (to be removed prior to the installation) Unlocking button Hose connector (unblocks the drain when connected) Hose (part no. 80415) Centrifuge door (front, left side) Clamp Quick coupling Condensate – rotor chamber 10 Lock nut (fasten by hand) Fig.
Page 28
8K(B) Set-up and Connection Connector for the cooling water inlet Connector for the cooling water outlet Fig. 5.4: Connections for the water cooling system Version 01/2009, Rev. 1.16 of 01/09/2011- sb Translation of the original operating manual...
8K(B) Using the Centrifuge Using the Centrifuge Initial Start-Up Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 "Set-up and Connection"). Switching the Centrifuge ON Press the mains power switch on the right side of the front (see Fig.
8K(B) Using the Centrifuge After frequent use, the rotor tie-down screw must be loosened by some turns, the rotor has to be lifted and fastened again. This must be done once a day or after 20 cycles. This ensures a proper connection between the rotor and the motor shaft.
8K(B) Using the Centrifuge Centrifugation with low capacity: The tubes must be installed symmetrically so that the buckets and their inserts are loaded evenly (see Fig. 6.2). Fig. 6.2: Permissible loading of the swing-out rotor 6.2.2.4 Adapters In order to ensure easy handling, even if vessels of various sizes are used, adapter systems were developed.
8K(B) Using the Centrifuge 6.2.2.6 Blood Bag Systems All six places on the rotor must be loaded with buckets. It is required to fill just two opposite buckets must be filled with one adapter for blood bags and two blood bag systems each.
8K(B) Using the Centrifuge Spincontrol Professional 6.3.1 Operating Panel The centrifuge is started directly via the operating panel. When the centrifuge is switched on, all of the operating keys and displays will be illuminated for a short time. It is now ready for operation.
8K(B) Using the Centrifuge 6.3.3 Manual Mode 6.3.3.1 Starting the Centrifuge The centrifuge is ready for operation when the start key is illuminated. Press the start key in order to start a centrifugation run. 6.3.3.2 Interrupting a Centrifugation Run Press the stop key in order to interrupt a centrifugation run. The centrifugation run will be terminated prematurely.
Page 35
8K(B) Using the Centrifuge 6.3.3.5 Standard Menu Fig. 6.6: Standard Menu This menu is displayed a few seconds after the centrifuge has been switched on. The following parameters can be displayed and modified: Speed In the upper section of the field, the set speed of the centrifuge is displayed.
Page 36
8K(B) Using the Centrifuge Continuous Run During the continuous run, the runtime of the centrifuge is unlimited and must be stopped manually. The centrifuge accelerates during the continuous run until the set speed is reached. Select the field “Runtime” and confirm the selection. The display flashes when it is activated Select the time 0:00:00 and confirm the selection.
Page 37
8K(B) Using the Centrifuge Identifying and adapting incorrectly set rotors The centrifuge automatically identifies the rotor that is being currently used. If the system identifies a different rotor than the one that is set, if there are no different buckets for this rotor, and if the speed has not been adapted manually, the rotor input will be adapted automatically.
8K(B) Using the Centrifuge 6.3.3.6 Profile Menu This menu shows the profiles that are used. A profile begins and ends with 0 speed. The vertical axis shows the speed and the horizontal axis shows the time. Changing the scale: Select the value that is to be changed. The activated display flashes.
Page 39
8K(B) Using the Centrifuge Process Standstill cooling Depending on the substances to be centrifuged, it may make sense to precool the centrifuge. The precooling prevents the cooled samples in the uncooled centrifuge from heating up to an inadmissible temperature. If the standstill cooling is activated, the centrifuge starts to precool after it is switched on.
Page 40
8K(B) Using the Centrifuge The automatic lid opening function is suppressed after a rapid cooling phase in order to prevent the system from reheating. An entry will interrupt the rapid cooling program. There will be no message when the set temperature is reached. If no new runtime is entered, the centrifuge will continue to run infinitely (continuous run).
Page 41
8K(B) Using the Centrifuge Lock In order to prevent any unauthorised use of the centrifuge, the following functions can be blocked: Saving of programs Changing of parameters Loading of programs Start key Blocking a function: Select the function that is to be blocked. The preceding functions will also be automatically selected (example: If the Parameter function is selected, the Save function will also be selected).
Page 42
8K(B) Using the Centrifuge Enter the interval time under “Time”, but take the restrictions into consideration (see below). Enter the desired acceleration under "RPM“ or "RCF", but take the restrictions into consideration (see below). The values are interdependent. For the first interval, a linear or quadratic rise can be selected under "QUAD/LIN“.
8K(B) Using the Centrifuge Brakeless deceleration as of a set speed If this function is active, the drive will be disconnected if the actual speed is below the set speed. As a result, the rotor decelerates and stops in a brakeless manner.
Page 44
8K(B) Using the Centrifuge Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed. The symbol " " will be displayed in the time field. Display Temperature unit Select between °C (Celsius) and °F (Fahrenheit).
8K(B) Using the Centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are currently being used. Info This menu provides information on the software versions that are used in this centrifuge.
8K(B) Using the Centrifuge 6.3.3.10 Changing the Contrast Press and hold the stop key and turn the function knob one notch to the left. A dialog box will be displayed. Adjust the contrast of the centrifuge display and confirm the change.
8K(B) Using the Centrifuge 6.3.4 Program Mode A program contains all data that are required for a centrifuge run. Attention: Temperature profiles cannot be created! With a program, certain sedimentation results can be repeated under identical conditions. Programs can be loaded, executed, modified, and deleted at any time.
8K(B) Using the Centrifuge 6.3.4.2 Saving a Program Enter the parameters that are to be included in the program. Select the option „Progr“ and confirm the selection. The program selection list will be displayed. Select a storage location from the program selection list.
Connection for a serial interface External signal active DC 24V, 0.5A max. (part no. 17701) Floating switch AC 250V max. 6A (part no. 17702) Standard for Sigma 8KB Version 01/2009, Rev. 1.17 of 27/01/2012 Translation of the original operating manual...
8K(B) Malfunctions and Error Correction Switching the Centrifuge OFF Open the centrifuge when it is not in use so moisture can evaporate. Press the mains power switch on the front of the right side. Malfunctions and Error Correction General Malfunctions Malfunctions are indicated by a dialogue box.
8K(B) Malfunctions and Error Correction 7.1.1 Emergency Lid Release In the event of a power failure, it is possible to manually open the centrifuge lid. Switch off the mains power switch and disconnect the power cord from the socket. Remove the plugs (1) at the right side panel, e.g. with a screw driver.
8K(B) Malfunctions and Error Correction Error Codes Error no. Kind of error Measure Note System error All these errors stop the allow to slow down centrifuge or cause it to power off/on decelerate brakeless 10-19 Speedometer error allow to slow down...
8K(B) Maintenance and Service Maintenance and Service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure. If corrosion or other damage occurs due to improper care, the manufacturer cannot be held liable or subject to any warranty claims.
8K(B) Maintenance and Service 8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time. All swing-out rotors applicable for this centrifuge and the angle rotor 12510 weigh more than 18 kg.
8K(B) Maintenance and Service Check the material regularly (at least once a month) for - cracks - visible damage of the surface - pressure marks - signs of corrosion - other changes. Check the bores of the rotors and multiple carriers.
8K(B) Maintenance and Service 8.1.6 Condenser (only air cooled centrifuges) In order to cool the refrigerant that is compressed by the refrigeration unit, a lamellar condenser is used. It is cooled by air. Dust and dirt obstruct the cooling flow of air. Dust on condenser pipes and lamellas reduces the heat exchange and thus the performance of the refrigeration unit.
This symbol means that it is not permissible to dispose of the unit among household trash. You can return these centrifuges free of cost to SIGMA Laborzentrifugen GmbH. Ensure that the unit is decontaminated. Fill in a declaration of decontamination.
8K(B) Technical Data Technical Data Manufacturer: S I G M A Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type Electr. Connection: see name plate see name plate Protection class: IP Code:: Connected load (kVA): Power consumption (kW): Max.
Adapter for 19 Culture tubes 15ml, cap max. Ø 23 mm e.g. no. 15115, Nunc-, Falcon-, Greiner- and Corning tubes, 1 set = 2 pcs. Not for Sigma 8KB Version 01/2009, Rev. 1.16 of 01/09/2011- sb Translation of the original operating manual...
Page 63
8K(B) Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 17777 Adapter for 8 Culture tubes 50 ml e.g. no. 15151, 1 set = 2 pcs. 13852 Adapter for 1000 ml bottles Ø 98 mm no. 15920, 15921, 1 set = 2 pcs.
Page 64
8K(B) Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 17677 Round carrier for 5 culture tubes 50 ml, e.g. no. 15151, Nunc, Corning, Falcon and Greiner, 1 set = 2 pcs. 17678 Round carrier for 5 culture tubes 50 ml with skirt or flat bottom, max.
Page 65
8K(B) Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 17651 Round carrier for 20 tubes 4 - 7 ml, flat bottom, max. Ø 15.5/18 x 50 - 75 mm, e.g. Monovette, Primavette, 1 set = 2 pcs.
Page 66
8K(B) Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 17692 Round carrier for 1 bottle with flat bottom approx. 125 ml, max. Ø 51 x 90 - 115 mm, e.g. no. 15125, 1 set = 2 pcs.
Page 67
Adapter for 12 Culture tubes 15ml, cap max. Ø 23 mm e.g. no. 15115, Nunc-, Falcon-, Greiner- , Corning tubes 1 set = 2 pcs. Not for Sigma 8KB incl. accessories Version 01/2009, Rev. 1.17 of 27/01/2012 Translation of the original operating manual...
Page 68
8K(B) Appendix Part no. Description Max. speed Max. (rpm) gravitational field (x g) 14167 Adapter for 8 round bottom tubes approx. 30 ml, max. Ø 25.5/29 x 90 - 100 mm e.g. no. 15029, 15030, 15032, 1 set = 2 pcs.
Page 69
8K(B) Appendix Part no. Description 15008 Reaction vials 1.5 ml, 100 pcs. per pack, suitable for 17653 15010 Polycarbonate tube 10 ml, Ø 16.1 x 81.1 mm, incl. screw cap, suitable for 14124, 14144, 14164, 17655 15014 Reaction vials made of polypropylene 0.4 ml, 100 pcs. per pack, suitable for 13000 15015 Centrifuge glass tube 10 - 12 ml, max.
8K(B) Appendix Part no. Description 15106 Centrifuge glass tube 97 ml, max. 4,000 x g, Ø 44 x 100 mm, graduated 1 up to 97 ml in 1 ml increments, suitable for 17690 15115 Culture tube Nunc, 15 ml, pointed bottom, polypropylene, incl. screw cap Ø 23 mm,...
8K(B) Appendix 11.2 Grafical Representation of the Rotors The graphical representation of the rotors shows the maximum and minimum radii of the accessories used. If necessary, the values must be manually calculated (see 11.4.1 "RCF “). Fig 11.1: Minimum and maximum radius of a swing- out rotor Fig.
Page 72
8K(B) Appendix Linear slope (curves 0 - 9) Curve 9 is a special case compared to the other curves. The centrifuge accelerates with maximum power. The runtime, until the set speed is reached, depends solely on the moment of inertia of the rotor.
8K(B) Appendix 11.3.2 Quadratic Curves The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers. Quadratic slope (curves 10 - 19) Curve 19 is a special case compared to the other curves. The centrifuge accelerates with maximum power. The runtime depends solely on the moment of inertia of the rotor.
8K(B) Appendix 11.4 Formulae - Mathematical Relations 11.4.1 Relative Centrifugal Force (RCF) The parameters speed, RCF, and the group rotor and radius cannot be specified independently. They are interrelated via the following formula: RCF = 11.18 x 10-6 x r x n2 If two values are given, the third value is determined by the equation.
Page 75
8K(B) Appendix Fig. 11.7: Speed-Gravitational-Field Diagram Version 01/2009, Rev. 1.17 of 27/01/2012 Translation of the original operating manual...
8K(B) Appendix Table of rotors and accessories with a different service life Rotors and accessories with a different service life If other no data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10,000 cycles. After 50,000 cycles, rotors must be scrapped for safety reasons.
8K(B) Appendix Resistance Data Resistance Data Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated Acetone Acrylonitrile Allyl alcohol Aluminum chloride AlCl saturated Aluminum sulfate Ammonium chloride...
Page 78
8K(B) Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Dioxane Dipropylene glycol (mono)methyl ether Ethyl acetate Ethylene chloride Ferrous chloride FeCl saturated Formaldehyde solution Formic acid Furfural...
Page 79
8K(B) Appendix Resistant at +20 °C - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Potassium hydroxide Potassium hydroxide Potassium nitrate Potassium permanganate KMnO Pyridine Resorcinol Silver nitrate AgNO Sodium bisulfite NaHSO Sodium carbonate...
Layout Plan (here: water cooled centrifuge, GMP version, part no. 10857)
Page 85
8K(B) Index Index Acceleration........................33, 37 Acceleration and deceleration curves................41, 71 Acceleration curve ....................37, 46, 71 Accessories for blood bags....................66 Accessories for micortiter, deepwell and filter plates ............62 Accessories for tubes 0,5 - 1000 ml (max. capacity 12 l), only for rotor 11806 ..... 64 Accessories for tubes from 0,5 - 1500 ml (max.
Page 87
8K(B) Index Flow rate........................27, 61 Form for the return of defective parts ................... 60 Formulae - mathematical relations ..................74 Freezing-over of the compressor ..................39 Function..........................43 Fuses........................... 26 Fuses have tripped ......................50 General malfunctions ......................50 Glass breakage......................
Page 88
8K(B) Index Lid seal sticks ........................50 Limited service life ....................... 18 Linear curves ........................71 Load-bearing bolts ....................... 56 Loading a program....................... 47 Local rules and regulations ....................9 Mains power switch......................11 Mains power switch off......................50 Maintenance ........................54 Malfunctions.........................
Page 89
8K(B) Index Parameter menu ........................38 Pathogenic substances..................17, 54, 55, 57 Pivot bearing........................56 Plastic accessories ......................55 Plastic wrench, size 10 ......................10 Potential hazard to the life and health .................. 14 Potentially hazardous situation .................... 14 Power consumption ....................... 12, 61 Power cord ..........................
Page 90
8K(B) Index Rotors, buckets and carriers ....................55 Rubber cushions......................55, 56 Rubber parts ........................17, 58 Runtime ........................35, 46, 71 Runtime field........................33 Runtime fine......................... 44 Runtime from set speed ....................... 43 Safety ..........................13 Safety and danger indications on the centrifuge..............15 Safety area ..........................
Page 91
8K(B) Index Storage conditions ....................... 21 Storage locations ......................... 61 Storage temperature ......................21 Stress-corrosion........................55 Structural changes....................... 17 Suitable accessories ...................... 30, 62 Supply voltage ........................26 Supporting rings........................70 Swing-out rotor ........................30 Switching the centrifuge OFF....................50 Switching the centrifuge ON....................
Page 93
Short Operating Instructions Pay attention to the detailed Operating Manual, especially to the safety instructions in chapter 3! Function knob Operating panel with display Mains power switch Name plate Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5).
Page 94
Short Operating Instructions Program Mode • Select the option "Progr" from the “Standard” menu and confirm the selection. The program selection list will be displayed . • Select the desired program from the list and confirm the selection. Press the start key to start a centrifugation run. Parameter values can be modified during the centrifugation run as described under "Manual Mode"...