Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Subscribe to Our Youtube Channel
Related Manuals for SYSMEX CyFlow Cube 8
Summary of Contents for SYSMEX CyFlow Cube 8
- Page 1 ® CyFlow Cube 8 Instrument Operating Manual Revision: Rev-27_2015-07-02 Issued by: Sysmex Partec GmbH Sysmex Partec GmbH Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 2 Phone: +49 (0) 3581 8746 0 Fax: +49 (0) 3581 8746 70 Mail: support@sysmex-partec.com Web: www.sysmex-partec.com Sysmex Partec GmbH 2 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 3: Table Of Contents
Intermediate cleaning process ............. 25 Sheath and Waste bottle ..............25 6. Measurement parameters................... 26 Sysmex Partec GmbH 3 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 4 Cube 8 Optics ................ 41 ® 9.4.4 CyFlow Cube 8 Fluidics ..............41 Notes ..........................42 Sysmex Partec GmbH 4 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 5: Introduction
Serial number (serial No.) of the CyFlow Cube 8 Your complete contact address Sysmex Partec GmbH 5 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 6: Presentation
(e.g. Howard M. Shapiro, Practical Flow Cytometry. Wiley 2002). Sysmex Partec GmbH 6 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 7: 2.2 In Flow Cytometry, What Is
Histograms sometimes show undesired signals in the lower channels, frequently called ´noise´ or ´background´. These signals may originate from cell fragments or other Sysmex Partec GmbH 7 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 8 The Z scale is dynamic and will adapt during the measurement to a scale of 1 (black) to the maximum overlaying event coordinate color-coded in red. Sysmex Partec GmbH 8 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 9 Sysmex Partec GmbH 9 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 10: Instrument Starting Procedure
Once the Cube started no further steps are necessary. You can directly start your measurements! Sysmex Partec GmbH 10 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 11 Login: USER (case sensitive) Password: Cube1 (case sensitive) Sysmex Partec GmbH 11 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 12: User Levels
To enter the CyView login with your personal Login name followed by “Work with CyView™. Sysmex Partec GmbH 12 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 13: Cyview Tm Controls
Instrument real time display of workload (L), on-board memory status (M), analysis volume (V) and analysis duration (D). Sysmex Partec GmbH 13 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 14: Cyview™ Instrument Setting Controls
Select CR – Mode to show “All events” or “Regions only” Confirm all modifications by pressing Accept Sysmex Partec GmbH 14 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 15: Results
Confirm all modifications by pressing Accept Result will be display in the “Results table” next to the “Region statistics” Sysmex Partec GmbH 15 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 16: Regions
Use arrow keys to switch between regions Confirm all modifications by pressing Accept Sysmex Partec GmbH 16 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 17: General - Properties Of The Instrument
Flow defines the speed of sample injection in µl/s Lights on switches light sources on/off Sysmex Partec GmbH 17 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 18: Measure - Definition Of Measure Modes
Automatic storage at the end of the measurement. Autosave does not function when the measurement is finished by the “end” button. Sysmex Partec GmbH 18 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 19: Measure Modes
Volumetric counting (VC) of volume Volumetric counting (VC) electrodes Cells in region Sysmex Partec GmbH 19 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 20 STOP condition (select the respective MaxCount function in the REGIONS register). Sysmex Partec GmbH 20 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 21 Schematic overview of the measure modes with a flash indicates clearing of the data. User selectable criteria in green, software-defined values in red Sysmex Partec GmbH 21 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 22: Cyview Tm Instrument/Measurement Settings
These settings are crucial for a correct verification of the instrument performance. Initialization batch, Work batch and Clean batch Sysmex Partec GmbH 22 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 23 At the end press “Continue”. The instrument will be cleaned and the priming process ends. Switch to “WORK” batch Sysmex Partec GmbH 23 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 24: Work Process
4010) Connect a sample tube with sheath fluid and press “Continue” (Order No.04-4007) Sysmex Partec GmbH 24 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 25: Intermediate Cleaning Process
Sheath bottle can be cleaned with sheath fluid or hypochlorite solution 0.5% (Order No. 04- 4012) Sysmex Partec GmbH 25 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 26: Measurement Parameters
This tool allows to increase the accuracy and precision of the acquired data. Sysmex Partec GmbH 26 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 27: 2D Plot Properties
The displayed parameters can be chosen from the drop down menu; giving the user the list of the activated parameters. Sysmex Partec GmbH 27 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 28: Region/Roi Properties
To set the ROI/region as a sorting gate, just by selecting the box. (This option is only available on the Cube equipped with Sorter flow cuvette). Sysmex Partec GmbH 28 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 29 Sysmex Partec GmbH 29 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 30 All + Region colors shows the events in the selected region(s) in color together with all events in pseudo 3D color Sysmex Partec GmbH 30 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 31: Compensation
Clear/Save and Load Compensation Confirm by clicking Accept Example of a color-crosstalk compensation of FL1 into FL2. Sysmex Partec GmbH 31 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 32: Typical Sample Analysis
During the data acquisition the total particle count and the analysis rate is indicated on the real-time count meters Sysmex Partec GmbH 32 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 33 A volume can be pre-selected Cells in region o A particle number can be pre-selected Sysmex Partec GmbH 33 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
-
Page 34: Keyboard/Mouse Combinations
Marks the region and moves it in all regionplots Region Central mouse in the visual area Sysmex Partec GmbH 34 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 35: Appendix
PPE (personal protection equipment) must be met for all users. Therefore, the system is marked with the following biohazard label: Warning: biohazards Sysmex Partec GmbH 35 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 36: Maintenance
Temperature 5-50 Humidity 20-85 % relative (non-condensing) Room Clean environment, no direct sun light Sysmex Partec GmbH 36 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 37: Disposal
After decontamination, the system has to be disposed according to the local regulations and laws. For further information, please contact your local distributor or Sysmex Partec. Sysmex Partec GmbH 37 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 38: Laser Safety
Laser radiation Class IIIb, if cover is removed and shutter is opened Additional Explanation Sysmex Partec GmbH 38 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 39: Technical Specifications
® Optical bench of a CyFlow Cube with 2 lasers, UV-LED and 6 parameters. Sysmex Partec GmbH 39 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 40: Cyflow ® Cube 8 System
Cube System is a completely closed system not producing any aerosols during cellular analysis. Sysmex Partec GmbH 40 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 41: Cyflow ® Cube 8 Optics
2x 1-litre integrated reservoirs for sheath fluid and waste BioSafety System Avoids sample droplets and sample cross contamination (computer controlled). Sysmex Partec GmbH 41 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 42: Notes
……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… ……………………………………………………………………………………………………… Sysmex Partec GmbH 42 / 43 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 43 Sysmex Partec GmbH Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
Need help?
Do you have a question about the CyFlow Cube 8 and is the answer not in the manual?
Questions and answers