Related Manuals for SYSMEX onCyt OC-300
Summary of Contents for SYSMEX onCyt OC-300
- Page 1 CyFlow™ Cube 6 V2m Interface - onCyt™ OC-300 automation add-on Operating Manual - Supplement With CyView™ 1.8 Doc. No.: CY-S-3061R-V2m_U1IFUEN | Rev: 002 | Rev. date: 04-13-2021 | EN | CN 2084...
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Page 3: Table Of Contents
Table of contents Table of contents Introduction ....................... 4 Scope of application .................... 4 About this document ................... 4 Installation ......................5 Installation and uninstallation ................5 External connection .................... 5 Software activation....................5 Specific CyView™ scripts ................... 6 Operation ......................7 Scope of Operation ..................... -
Page 4: Introduction
1 Introduction 1 Introduction 1.1 Scope of application CyFlow™ Cube 6 V2m online monitoring interface consists of a CyFlow™ Cube 6 V2m and an additional OC-300 automation unit by onCyt™. It is operated via CyView™ software as well as onCyt™ cyOn-300 software. The interface intends to connect the CyFlow™... -
Page 5: Installation
2 Installation 2 Installation Carefully select the place where to position and operate the system, according to instructions of the CyFlow™ Cube 6 V2m as well as the onCyt™ OC-300 automation unit. After placement the system should not be moved without disconnecting its components. 2.1 Installation and uninstallation Installation, connection, disconnection and uninstallation may only be performed by Authorised service personnel and in conformity with the applicable national rules and... -
Page 6: Specific Cyview™ Scripts
Make sure to only use CyView™ scripts specifically designed for the CyFlow™ Cube 6 V2m with connected OC-300 automation unit. In case of doubt, please contact your local Sysmex representative. CyFlow™ Cube 6 V2m Interface - OnCyt™ | Addon - Operating Manual | April 2021... -
Page 7: Operation
Microbiology AG 3.1 Scope of Operation The following items are available through Sysmex and distributors authorised by Sysmex, also the list should help the user identify what is needed for operation of the system. Visit our website http://www.sysmex-partec.com or contact your local Sysmex representative for further information about our products. -
Page 8: Software
3 Operation Item Content REF No. Count Check Beads green 50 tests 05-4011_R Calibration Beads 0.5 µm concentrate 10 ml 05-4005 Sample tubes 3.5 ml 500 piece 04-2000 Small Volume Sample tubes 1 ml 100 piece 04-2010 Waste bottle 5 l 5000 ml empty bottle 04-200-1058 Table 2: Additional Reagents and Accessories for Operation... -
Page 9: Start-Up
3 Operation 3.3 Start-Up 3.3.1 Reagent Preparation Make sure to have the following Accessory and Staining solutions available for connection to the OC-300 automation unit. For precise estimates of volumes necessary for the experiment you select, please refer to the onCyt™ volume reference sheet for the respective experiment. -
Page 10: Start-Up Of The Flow Cytometer
3 Operation 3.3.2 Start-Up of the flow cytometer Requirements • The Sheath Fluid bottle is filled and connected to the device. • The Waste bottle is empty and connected to the device. • The OC-300 automation unit is connected to the device. •... -
Page 11: Handling Of The Oncyt™ Oc-300 Automation Unit
3 Operation 3.3.3 Handling of the OnCyt™ OC-300 automation unit Requirements • The Waste bottle is empty and connected to the device. • The OC-300 automation unit is connected to the CyFlow™ Cube 6 V2m. • Insert an empty small volume sample tube into a sample tube and connect it to the sample port. -
Page 12: Shutdown
3 Operation 3.4 Shutdown The Shutdown process cleans and empties all lines. Requirements • The Waste bottle is empty and connected to the device. • The Sheath Fluid container is filled • The OC-300 automation unit is connected to the CyFlow™ Cube 6 V2m •... -
Page 13: Troubleshooting
For general faults, please also refer to the CyFlow™ Cube 6 V2m Operating Manual. If the fault you are experiencing is not described or the remedy could not solve your problem, please contact your local Sysmex representative. 4.1.1 Measurements and data acquisition... -
Page 14: Cystain™ Baccount Qc Procedure
4 Troubleshooting Fault Remedy The OC-300 automation unit Make sure the analysis with cyPlot-300 software is set up script stops at the [Analyse] correctly and the software communication is running. command If no cyPlot-300 software real-time analysis is required, please select an OC-300 automation script without the [Analyse] command. - Page 15 4 Troubleshooting Fault Remedy Unusual high background 1. Remove the Inline filter. during measurement and /or 2. Fill the Sheath Fluid bottle with Hypochlorite solution failling background test and reconnect it. within the Quality Check 3. Switch to [Analyze All] and run three times 1200 µl report Hypochlorite solution at full speed.
- Page 16 4 Troubleshooting Fault Remedy Count Check Beads green Shake the bottle vigorously (e.g. by vortexing) and repeat measurement is not within the measurement. ± 20 % range of the LOT- Check the date of expiry of the Count Check Beads specific concentration stated green.
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