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Summary of Contents for SYSMEX Ploidy
- Page 1 Ploidy Analyser ™ with CyFlow Robby 6 Autoloading Station Operating Manual ™ ™ Ploidy Analyser (with CyView™ software) For CyFlow Robby 6 Autoloading Station with CyFlow Doc. No.: CY-S-3080-6PAIFUEN | Rev: 002 | Rev. date: 11-09-2020 | EN | CN 1671...
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Page 3: Table Of Contents
Table of contents Table of contents Identification ......................5 Product information ..................5 Manufacturer ..................... 5 Presentation ......................6 What other manuals are available? ..............6 What standards were applied for CE compliance? ........6 What typographical conventions are used in this manual? ......6 2.3.1 Symbols in this document ................ - Page 4 Table of figures Table of figures ™ Figure 1: CyFlow Robby 6 Autoloading Station Initialization ......... 10 Figure 2: User Login window ................... 10 Figure 3: “PRIME” button (bright highlighted), “WORK” button and “CLEAN” button ..11 Figure 4: Go to “Measure” tab and choose the measure mode “Plate_10-40” (Example) 11 Figure 5: “WORK”...
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Page 5: Identification
™ Name CyFlow Robby 6 Autoloading Station Software please see labelling of ™ CyFlow Ploidy Analyser (with CyView™ software) CY-S-3080-6 ™ Versions To be used in combination with CyFlow Ploidy Analyser only! CY-S-3039_V1 with CY-S-3080-6 CY-S-3039_V2 with CY-S-3080-6 CY-S-3039_V3 with CY-S-3080-6 ™... -
Page 6: Presentation
2 Presentation 2 Presentation ™ ™ The CyFlow Ploidy Analyser can be operated with or without the CyFlow Robby 6 Autoloading Station. ™ This manual is only valid for CyFlow Robby 6 Autoloading Station in combination with ™ CyFlow Ploidy Analyser with CyView™ software. -
Page 7: Symbols In This Document
2 Presentation 2.3.1 Symbols in this document To make warnings more accessible, the following symbols are used to reference the kind of hazard Hazard Meaning symbol General hazards Biological hazards Electrical hazards Beware of electrical currents and electrical shocks. 2.3.2 Safety information Safety information and warnings are marked by symbols and signal words. - Page 8 2 Presentation CAUTION Names the source of the hazard Describes the consequences of the hazard. This could result in minor injury. • Specifies the steps that must be taken to eliminate the hazard. NOTICE Names the source of the hazard Describes the consequences of the hazard.
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Page 9: Safety
3 Safety 3 Safety 3.1 General hazards CAUTION Moving sample needle in the autoloader When reaching into the autoloader tray, cutting injuries of fingers could occur. • Do not reach into the autoloader tray. Electrical hazards Please place the device in such a way, that the wiring is easily accessible to unplug and does not suffer damages in any way. -
Page 10: Instrument Starting Procedure
The power supply switch is found at the back of the CyFlow Ploidy Analyser next to the ™ main supply cable. The CyFlow Ploidy Analyser is, in default, set in a stand-by mode. ™ The full activation of the CyFlow Ploidy Analyser requires pressing the button on the top of the machine. -
Page 11: Priming / Initialization
5 Priming / Initialization 5 Priming / Initialization 5.1 Priming / Initialization process There is a PRIME mode available as a Start Up process for the device. This process includes cleaning and filling tubes with Sheath Fluid. If a new original CFG-file will be loaded the PRIME mode is always selected. -
Page 12: Work Process
5 Priming / Initialization 4. Connect a Sample Tube with Decontamination Solution (violet solution, Order No. 04-4010_R), press “Continue” and wait until the system finished. 5. Remove the Sample Tube, press “Continue” and wait until the system has finished. 6. Connect the Sample Tube again, press “Continue” and wait until the system has finished 7. -
Page 13: Shut Down Process
5 Priming / Initialization 5.3 Shut down process For the cleaning of the sample port a separate cleaning mode is available. The detail procedure would be the following. Figure 6: "CLEAN" is red highlighted 1. Select the “CLEAN” button by clicking on the “CLEAN” button. 2. -
Page 14: Measurement With The Autoloading Station
6 Measurement with the Autoloading Station 6 Measurement with the Autoloading Station 6.1 Measure mode ™ Each configuration file for the CyFlow Robby 6 Autoloading Station offers a certain number of measure modes. • Select the registry page “MEASURE” and choose one of the default measure modes. -
Page 15: Figure 8: Changeable Parameters Of Work Step
6 Measurement with the Autoloading Station using the Sample Port (manual mode) before starting Sample Analysis with delivery from the Autoloader tray (Automate mode). The other measure modes deliver samples from the Autoloader tray of the Robby 6 Autoloading Station and define different ranges of sample volume. -
Page 16: General Procedure
6 Measurement with the Autoloading Station 6.2 General Procedure 1. Open a configuration file 2. Select a measure mode for the manual operation (for example “Analyze all” → go to registry page “MEASURE” and selected the mode) 3. Connect a Sample Tube filled with 1 ml of sample to the manual sample port. A distinct sound will occur, if the Sample Tube is inserted correctly. -
Page 17: Figure 9. Please Insert A Tray
6 Measurement with the Autoloading Station Figure 10. Please change tray or tray Figure 11. Select applicable tray Figure 9. Please insert a tray layout layout 13. The layout editor will open (layout format depends on selected tray format: 96-well or tubes (see below)), to define the position of samples and the names the individual samples in a 96-well plate or the tube rack. - Page 18 6 Measurement with the Autoloading Station 16. Start the analysis, by pressing “Start”. ™ The CyFlow Robby 6 Autoloading Station will immediately move to the first selected tray position to take-up the defined sample volume of the first sample. Next in sequence, the sample will be forwarded towards the flow cuvette.
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Page 19: Changing A Rack
6 Measurement with the Autoloading Station 6.3 Changing a rack Three rack types are available: • Rack for 2 x 48 well plates (see picture “48 wells”) • Rack for 2 x 96 well plates (see picture “96 wells”) • Tube rack (see picture “Tubes”) To change a rack to another rack, pull out the tray slide from the autoloader tray and... -
Page 20: Cleaning Procedure
™ The sample port of the CyFlow Ploidy Analyser can be cleaned using the Cleaning Solution (green solution, Order No 04-4009_R) followed by a Sample Tube with Sheath Fluid. This procedure allows to reduce significantly the cross contamination and reduce the background. -
Page 21: Sheath Fluid And Waste Bottle
7 Cleaning Procedure 7.3 Sheath Fluid and Waste bottle CAUTION Direct contact with biohazardous material Possibly biohazardous material like samples and Waste bottle contents may cause severe illness. • Strict guidelines, international, as well as national regulatory standards such as PPE (personal protection equipment) must be met for all users. -
Page 22: Maintenance
8.2 Preventive maintenance and service Installation, Service and Preventive Maintenance are all part of the authorised service personnel responsibilities, please contact your local Sysmex representative. If you have any questions about the content of this operating manual or the use of the device, contact your local Sysmex representative. -
Page 23: Disposal
8 Maintenance 8.4 Disposal After decontamination, the system must be disposed according to the local regulations and laws. For further information please contact your local Sysmex representative. ™ CyFlow Robby 6 Autoloading Station | Operating Manual | September 2020 | Revision 002... -
Page 24: Technical Data
9 Technical data 9 Technical data Specification Description Dimensions Length: 360 mm Width: 370 mm Height: 270 mm Weight 12 kg Maximum sound <70 dBA Power 100 to 240 V AC 50/60 Hz 72 VA Overvoltage category 2/II Degree of protection IP 20 Means of protection Class III (safety extra low voltage)
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