SYSMEX XP Series Instructions For Use Manual
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SYSMEX XP Series Instructions For Use Manual

SYSMEX XP Series Instructions For Use Manual

Automated hematology analyzer
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Table of Contents

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CHAPTER 1
Introduction
CHAPTER 2
Safety Information
CHAPTER 3
Design and Function
CHAPTER 4
Reagents
CHAPTER 5
Initial Operation
CHAPTER 6
Operation
CHAPTER 7
Sample Analysis
CHAPTER 8
Display and Output of Analysis Results
CHAPTER 9
Quality Control
CHAPTER 10 Calibration
CHAPTER 11 Instrument Setup
CHAPTER 12 Cleaning and Maintenance
CHAPTER 13 Troubleshooting
CHAPTER 14 Technical Information
Index
© SYSMEX CORPORATION 2012-2014
Automated Hematology Analyzer
Instructions for Use
KOBE, JAPAN
XP series
XP-300
Code No. AC580857
PRINTED IN JAPAN
Date of Last Revision: May 2014
Software Version: 00-12 and onwards

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  • Page 1 CHAPTER 11 Instrument Setup CHAPTER 12 Cleaning and Maintenance CHAPTER 13 Troubleshooting CHAPTER 14 Technical Information Index KOBE, JAPAN Code No. AC580857 PRINTED IN JAPAN Date of Last Revision: May 2014 © SYSMEX CORPORATION 2012-2014 Software Version: 00-12 and onwards...

  • Page 3: Table Of Contents

    Prepare internal printer paper (Printing Paper No. 3) ..5-11 Peripheral devices (optional) ........... 5-13 Connection of a host computer, printer and handheld bar code reader ............... 5-13 Connection of power cable ..........5-14 5.10 Basic instrument settings ..........5-15 Sysmex XP-300...
  • Page 4 10.4 Automatic calibration ............10-4 10.5 Manual calibration ............10-9 10.6 Printing calibration history ..........10-12 Instrument Setup ...........11-1 11.1 Introduction ..............11-1 11.2 Possible settings .............. 11-3 11.3 Password setting ............. 11-9 11.4 Print settings ..............11-11 Sysmex XP-300...
  • Page 5 Print formats ..............14-9 14.4 Connecting the bar code reader ........14-12 14.5 System of units .............. 14-19 14.6 Functional description ............ 14-21 14.7 Warranty ................ 14-48 14.8 Appendix ................ 14-49 14.9 GNU GENERAL PUBLIC LICENSE ......14-51 Index Sysmex XP-300...
  • Page 6 Table of Contents Sysmex XP-300...

  • Page 7: Introduction

    Introduction 1. Introduction Thank you for purchasing the Sysmex XP-300 Automated Hematology Analyzer. Before operating this instrument, carefully read this manual. Keep this manual for further reference. The XP-300 is a compact instrument, which is designed for easy operation. For each operating step, the user can adapt the instrument to his/her needs or existing laboratory conditions by selecting the appropriate settings.
  • Page 8 Ordering of supplies and replacement parts If you need to order supplies or replacement parts, please contact your local Sysmex representative. Service and maintenance Please contact the Service Department of local Sysmex representative. Training courses For further training information, please contact your Sysmex representative.

  • Page 9: Intended Use

    Caution! If this sign is ignored and the instrument is operated incorrectly, a potentially hazardous situation could result in injury to the operator, adverse effect on analysis results or property damage. Sysmex XP-300...

  • Page 10: Trademarks

    Note: Indicates information which will be useful in operating the instrument. 1.3 Trademarks • Sysmex, EIGHTCHECK, CELLCLEAN, CELLPACK, STROMATOLYSER and SNCS (Sysmex Network Communication System) are registered trademarks of SYSMEX CORPORATION. • ETHERNET is a registered trademark of Xerox Corporation.

  • Page 11: Analysis Parameters

    Absolute count of WBC-small cells (the lymphocytes) in 1 μL of whole blood • MXD# (W-MCC): WBC-Middle Cell Count Absolute count of WBC-middle cells (the mixed population of EO, BASO and MONO) in 1 μL of whole blood Sysmex XP-300...
  • Page 12 • MPV: Mean platelet volume Mean volume of platelet (fL) • P-LCR: Large platelet ratio Ratio (%) of large platelet volume exceeding 12 fL to the platelet volume • PCT: Plateletcrit Ratio (%) of whole PLT volume in whole blood Sysmex XP-300...

  • Page 13: Safety Information

    Using the instrument any further bears the risk of short circuit, fire, electrical shock or personnel injury. Contact your local Sysmex representative for inspection. • Do not spill liquids such as blood samples or reagent, nor put metal objects such as staples or paper clips on the instrument.

  • Page 14: Installation

    (e.g. unshielded intentional RF sources), as these may interfere with the proper operation. Note: Please always run the instrument with the front cover closed. The instrument does not comply with the standards mentioned above while the front cover is left open. Sysmex XP-300...

  • Page 15: Avoiding Infections

    • Control blood must be regarded as potentially infectious. Wear protective garments and gloves when performing quality control. Sysmex XP-300...

  • Page 16: Handling Of Reagents

    • Reagents must not be used after their expiration date. • Handle reagents gently to avoid bubbling. Do not shake! Do not use directly after transportation. • Take sufficient care to prevent the reagent from spilling. If reagent is spilt, wipe up with a damp cloth. Sysmex XP-300...

  • Page 17: Maintenance

    When cleaning the touch panel surface, use a “soft cloth dampened with ethanol”. • If the instrument is not operated every day, a “Blank Error” may occur. In this case, follow the instructions displayed in the action message screen. Sysmex XP-300...

  • Page 18: Disposal Of Waste Fluid, Waste Materials, And The Device

    A battery is incorporated in the right side interior of the instrument in order to keep the stored data. When disposing of the instrument, remove the battery. Disposal procedures for batteries should meet the requirements of all applicable local regulations. Battery Right side interior of the instrument Sysmex XP-300...
  • Page 19 Warning! • Do not dispose the instrument, accessories and consumables via public recycling! • Incineration of contaminated parts is recommended! Contact your local Sysmex service representative and receive further instructions for disposal! Follow local legal requirements at all times. Caution!
  • Page 20 • If required, you may afterwards remove normal contaminations with commercial neutral detergent, in case these could not be removed by the decontaminant. • As a last step the instrument shall be dried with a dry one-way cloth. Sysmex XP-300...

  • Page 21: Warning Labels On The Instrument

    • For the continued protection against risk of fire, replace only with fuse of the specified type and current rating. • Never open the instrument’s cover on the back when the main power switch is ON. Do not open this cover unless absolutely necessary. Sysmex XP-300...
  • Page 22 • Static electricity may damage the electronic circuit via the connectors on the rear panel. Do not touch connector pins with hands. • Turn the main power switch OFF before inserting or removing connectors or program cards. 2-10 Sysmex XP-300...
  • Page 23 When opening the detector cover to clean the TD aperture, follow the instructions in “12.12 Clean aperture of TD chamber (drain TD chamber)”. Because there is a risk of electric shock, do not open this cover for any other purpose. Sysmex XP-300 2-11...
  • Page 24 The printer head becomes hot. Use caution. Caution! Static electricity may damage the printer head. Do not touch with your hands. Caution, risk of electric shock The LCD inverter becomes a high voltage exceeding 1 kV. Do not touch while power supply on. 2-12 Sysmex XP-300...

  • Page 25: Operators

    • Personnel who have less experience operating the instrument should receive guidance and assistance from an experienced operator. • If the instrument requires service, consult the Instructions for Use for troubleshooting information. If additional repair is required, contact your Sysmex technical representative. Sysmex XP-300 2-13...
  • Page 26 Safety Information 2-14 Sysmex XP-300...

  • Page 27: Design And Function

    The sample probe is used to aspirate sample in the whole blood and pre-diluted modes. (3) Start switch This switch starts whole blood mode analysis and pre-diluted mode analysis. (4) Color LCD screen Displays the sample ID number, analysis results, instrument status, error messages, and menus. Sysmex XP-300...

  • Page 28: Right View

    Avoid turning this switch ON and OFF continuously in short duration. This will overload the fuse and may cause the fuse to blow. (3) AC power supply connector Using the provided power cord, this connector supplies power from the outlet. Sysmex XP-300...

  • Page 29: Left Side Panel

    Prevents reagent, etc. from flowing into the vacuum pump of the compressor when an error occurs with the instrument. (2) Regulator Regulates pressure to 0.05 MPa. (3) Bellows unit Regulates vacuum to –0.0333 MPa. (4) Air filter Prevents dirt and dust from entering the bellows unit. Sysmex XP-300...

  • Page 30: Rear Side

    Connects a handheld bar code reader. Socket for graphic printer (option) Connects an optional graphic printer. Socket for LAN (SNCS service) Connector for communicating with the Sysmex Network Communication System (SNCS) service. (5) Card slot Inserts a program card specified by Sysmex. Information Use only program cards specified by Sysmex.

  • Page 31: Front Interior

    Makes volumetric measurement of aspirated blood. (3) Rinse cup Cleans the sample probe. (4) WBC/HGB lyse (STROMATOLYSER-WH) Measures WBC/HGB. (5) Contrast adjuster Adjusts the contrast of the LCD screen. (6) Internal printer Prints analysis data, error logs, etc. Sysmex XP-300...

  • Page 32: Left Side Interior

    (1) Waste chamber (2) Pneumatic unit (1) Waste chamber Collects waste from transducers and mixing chambers. (2) Pneumatic unit Supplies pressure and vacuum. Note: Do no open the instrument cover unless your Sysmex service representative asks you to. Sysmex XP-300...

  • Page 33: Reagents

    4.1 General information All reagents used in this instrument are exclusively for use with Sysmex equipment. Do not use them for any other purpose. Please follow the warnings for safe handling and usage of each reagent as given on the reagent packaging, package insert and the instructions for use.

  • Page 34: Stromatolyser-Wh

    • STROMATOLYSER-WH is solely intended for use with Sysmex reagents and analyzers. • If other reagents are used, the product performance of Sysmex instruments cannot be guaranteed. • Do not use any left-over STROMATOLYSER-WH. Always use new STROMATOLYSER-WH. • Take care to prevent dust, dirt, bacteria, or other materials from entering the reagent after it is opened.

  • Page 35: Cellclean

    (show the label where possible). Information Storage and shelf life after first opening • Store CELLCLEAN in a dark place at 1 - 30°C. • The expiration date is shown on the outer packaging. • Avoid exposure to direct sunlight. Sysmex XP-300...

  • Page 36: Eightcheck-3Wp

    Storage and shelf life after first opening • Store EIGHTCHECK-3WP in a dark place at 2 - 8°C. • The expiration date is shown on the reagent’s packaging. • Once opened, this reagent should be used within 7 days. Sysmex XP-300...

  • Page 37: Symbols Used On The Labels

    Consult instructions for use Keep away from rain Temperature limitation Stacking limit by number Biological risks Fragile; handle with care Use by Corrugated recycles Batch code Serial number Catalogue number The design of the symbols may differ from the actual product. Sysmex XP-300...

  • Page 38: Reagent Specifications

    EDTA-2K 0.2 g/L STROMATOLYSER-WH 500 mL×3 2 - 35°C 15 - 30°C 90 days Organic quaternary ammonium salts 8.5 g/L Sodium chloride 0.6 g/L CELLCLEAN 50 mL 1 - 30°C 15 - 30°C – Sodium Hypochlorite (available chlorine concentration 5.0%) Sysmex XP-300...

  • Page 39: Initial Operation

    5. Initial Operation 5.1 Introduction This product is an In-vitro diagnostic medical device. A Sysmex service representative is responsible for unpacking, installing, and initial setup of the product to ensure its proper and safe operation. The next several pages will give some essential information on this instrument.

  • Page 40: Before Installation

    Float Switch No. 23 Assy 367-1051-9 Tray No. 20 442-5338-7 Tube Polyurethane 4 mm ID × 6 mm OD 442-5340-5 Tube Polyurethane 6 mm ID × 9 mm OD 423-1776-2 Mini-pet No. 10 (1 mL) 933-3601-9 Transducer Brush No. 1 Sysmex XP-300...
  • Page 41 Fuse 250V 4A ST4-4A-N1 – – AX880901 Fuse 250V 3.15A 50T032H – 266-6743-4 Clamp LWS-85-2.5W BJ312875 Printing Paper No.3 Note: Item No. 4 (973-3041-7 Float Switch No. 23 Assy) is pre-installed in the main unit front interior at the factory. Sysmex XP-300...

  • Page 42: Preparation Of Installation Location

    You may need some more desktop space if an optional graphic printer is connected. The dimensions of the instrument are shown below. The power cord is 1.8 m long. Width (mm) Depth (mm) Height (mm) Weight (kg) Main unit Sysmex XP-300...

  • Page 43: Removal Of Packing Tape

    2) With the fixed and rotary valves separated sufficiently, remove the protection sheets. 3) Return the SRV and the SRV fixing screw to their original positions. 4) Set the provided Tray No. 20. SRV fixing screw Tray No. 20 Protection sheets Sysmex XP-300...
  • Page 44 (5) Remove the pneumatic unit fixing screws (2 pcs). Keep these screws for future use. Fixing screws (6) Remove the rubber caps of the reagent connection nipples. Keep these rubber caps for future use. (7) Remove the factory-installed protective plate. Rubber caps Protective plate Sysmex XP-300...

  • Page 45: Connection Of Reagents And Waste Bottle

    • After unpacking, be sure not to allow dust or dirt to come into the reagent. Use up CELLPACK within 60 days from the unpacking date, and STROMATOLYSER-WH within 90 days. Note: When using the CELLPACK (10 L), a separate cubitainer spout kit is required. Contact your Sysmex service representative. Sysmex XP-300...
  • Page 46 • Avoid setting the diluent (CELLPACK) at a level higher than the instrument. Otherwise, the reagent may flow into the vacuum line, possibly damaging the instrument. • After connecting the tube, do not pull it by force for reagent replacement. Sysmex XP-300...
  • Page 47 • The maximum waste line length is 6 m. Since waste is removed from the system by pressurized air, the waste tank may be located at the same height as, or lower than, the drain outlet nipple. Otherwise the waste may flow back into the system, which will result in a malfunction. Sysmex XP-300...
  • Page 48 Drain outlet nipple Tube polyurethane 6 mm ID × 9 mm OD Clamp LWS-85-2.5W Waste container Pass the tubing through here. Caution! When bundling the tubings using the clamp, its sharp edges can cause injury. Use caution. 5-10 Sysmex XP-300...

  • Page 49: Prepare Internal Printer Paper (Printing Paper No. 3)

    If the cover is not securely closed, a printing error or incorrect output may result. Printer head Caution, Hot! The printer head becomes hot. Use caution. Caution! Static electricity may damage the printer head. Do not touch with hands. Sysmex XP-300 5-11...
  • Page 50 Initial Operation (6) Cut off any printer paper extending from the upper part of the printer. (7) Close the front cover of the main unit. 5-12 Sysmex XP-300...

  • Page 51: Peripheral Devices (Optional)

    When using a host computer or printer, see “Host Output” or “Printer” in “11.2 Possible settings” for settings relevant to data output. Refer to “14.4 Connecting the bar code reader” and the bar code reader manual for detailed information on the connection of a bar code reader. Sysmex XP-300 5-13...

  • Page 52: Connection Of Power Cable

    Initial Operation 5.9 Connection of power cable Connect the provided power cord to the power supply outlet. Power cord Connect to the power supply outlet Warning! Be sure to ground the instrument. Improper grounding may cause electrical shock. 5-14 Sysmex XP-300...

  • Page 53: Basic Instrument Settings

    Note: If a password has been set, the password input screen will appear. Enter the password. No password is set when the instrument is shipped; the password input screen will not appear, until a password is set. Sysmex XP-300 5-15...
  • Page 54 Press the [OK] button to return to the Main screen without changing the settings. Press the [Cancel] button to close the dialog and continue to change settings. For details about confirmation dialogs, see “Dialog screen” in “6.1 Screen display”. 5-16 Sysmex XP-300...
  • Page 55 LCD backlight illuminance will decrease automatically to conserve electric power (backlight dimmer timer function). Touch any portion on the LCD screen to Contrast adjuster return to a normally illuminated LCD screen. Underside of LCD Sysmex XP-300 5-17...
  • Page 56 Initial Operation 5-18 Sysmex XP-300...

  • Page 57: Operation

    LCD screen also displays the operation status and analysis progress. Main screen/Menu screen After turning the power ON, the Sysmex logo appears, and the startup operation is performed. Then the Main screen will appear. Analysis operation is performed on the Main screen. When the [Menu] button on the Main screen is pressed, the Menu screen will appear.
  • Page 58 Basic operation area The messages and the buttons are displayed in the Basic Operation area at the bottom of the LCD screen. Press the corresponding button to execute a basic menu operation and the cursor operation. Sysmex XP-300...
  • Page 59 Press the selection to input that value. If all of the options Type 2 cannot be displayed on the screen, the [more] button is displayed in Type 3 the options area. Press the [more] button to change the displayed options. Type 4 more Sysmex XP-300...
  • Page 60 × 2 A BCD Inputs “e” (not confirmed). A BCD e Confirms “e”. Ent. Confirms complete entry. Closes numerical keypad. Ent. Note: While the alpha or numerical keys are displayed on the screen, other buttons cannot be operated. Sysmex XP-300...

  • Page 61: Menu Tree

    Calibration LCD Menu Print Error Log Str.Data SRV reset Chg.Reag Auto Calib. Maint. Manual Calib. Calib. Print Cal.His. Settings System Date/Time QC Chart Patient Limits PU Sleep Quality Control Product ID Host Output Printer Network Password Setting Print Settings Sysmex XP-300...

  • Page 62: Signal Tones

    Pressing the start switch, the instrument will return to “Ready” status. Manual stop function This instrument stops the pneumatic unit when the [PU Sleep] button on the Menu screen is pressed. Pressing the start switch, the instrument will return to “Ready” status. Sysmex XP-300...

  • Page 63: Timer Functions

    6.7 SNCS functions The Sysmex Network Communication Systems (SNCS) is a support service that connects the Customer Support Center and customers’ products via the web for the online provision of remote instrument maintenance and quality control.

  • Page 64: Maintenance Report

    You need to sign up the SNCS service contract to use the SNCS service. For details about the SNCS service contract, contact your local Sysmex service representative. Online QC When you perform a quality control analysis, the analysis result is automatically transmitted to the SNCS server together with the QC file and instrument information.

  • Page 65: File Transmission

    Press the [File Send] button to transmit the saved screens to the SNCS server. This is convenient when explaining the displayed content to your Sysmex service representative. You cannot use this function when the [PS] button is not displayed on the screen.

  • Page 66: Emergency Call

    An immediate notice is transmitted to the SNCS server. Note: The SNCS function buttons ([PS] button, [File Send] button and [EMG.Call] button) are valid when the SNCS service setting is made. The setting must be made by a Sysmex service representative only. 6-10 Sysmex XP-300...

  • Page 67: Sample Analysis

    • Pre-diluted (PD) mode This mode is used to analyze micro samples (samples of smaller volumes) such as pediatric blood collected from the earlobe or fingertip. Analyzes a sample which has been diluted to 1:26 using a micro tube. Sysmex XP-300...

  • Page 68: Flowchart Of Analysis Procedure

    Page 7-8 Page 7-17 Display of analysis results • Latest sample Page 8-1 • Stored data Page 8-11 Printout of analysis results • Latest sample Page 8-10 • Stored data Page 8-19 End of operation (Shutdown) Page 7-28 Sysmex XP-300...

  • Page 69: Checks Prior To Operation

    (2) Check to see that the paper is properly aligned. Information Use only printer paper recommended by Sysmex. Inspection of reagents Check to see that the reagents needed for the number of the samples to be processed for the day are available. If the number available is such as might become short during the day, make ready the reagents for use in replenishment.
  • Page 70 No., expiration date, name of person who replenished. Such a record would come in handy. Inspection of waste If waste is collected in the trap chamber on the left side of the unit and the waste tank (when provided), discard the waste. Sysmex XP-300...

  • Page 71: Switching On

    SRV cleaning. last maintenance. Note: If an error is detected during the self-check, an error message appears on the LCD screen. Turn the instrument main power switch OFF. If the error persists, contact your Sysmex service representative. Sysmex XP-300...

  • Page 72: Background Check

    Press the [Result] button and the background check result will appear. Note: “Blank Error” may occur, if the instrument is not operated for several days. 7.6 Quality control Information Always perform a quality control prior to operation as described in “9. Quality Control”. Sysmex XP-300...

  • Page 73: Specimen Requirements

    The degree of change is variable depending on the specimen and the temperature at which it is stored. These changes are largely prevented by storage at 2 - 8ºC. Sysmex XP-300...

  • Page 74: Analysis In Whole Blood (Wb) Mode

    Note: All performance claims given in this manual were generated using specimens in EDTA anticoagulant. Results may be affected by the use of other anticoagulants. Therefore, each laboratory should develop protocols for handling specimens collected in their anticoagulants. Sysmex XP-300...
  • Page 75 Also, sample ID “0” will not be incremented automatically. • A sample ID cannot be entered when the pneumatic unit operation is stopped. Sysmex XP-300...
  • Page 76 (2) Enter the sample ID. During the sample ID input, the most right character can be deleted by pressing the [C] button. (3) Press the [Ent.] button. The sample ID is defined, and the status changes to Ready for starting analysis. 7-10 Sysmex XP-300...
  • Page 77 ID after entry. For details about check digits, see “ID Bar code specifications” in “14.4 Connecting the bar code reader”. • Do not use any other characters than alpha- numerics, hyphens and space on the bar code label. Sysmex XP-300 7-11...
  • Page 78 (2) Enter the operator ID. During the operator ID input, the most right character can be deleted by pressing the [C] button. (3) Press the [Ent.] button. The dialog closes and the entered operator ID is registered. 7-12 Sysmex XP-300...
  • Page 79 ID after entry. For details about check digits, see “ID Bar code specifications” in “14.4 Connecting the bar code reader”. • Do not use any other characters than alpha- numerics, hyphens and space on the bar code label. Sysmex XP-300 7-13...
  • Page 80 (2) Select an operator ID. The operator ID is defined, and the status changes to [Ready] for starting analysis. If the operator ID you want to select is not displayed, press [more] to display all other operator IDs that are registered. 7-14 Sysmex XP-300...
  • Page 81 (3) Remove the cap while taking care not to allow blood scatter. (4) Set the tube to the sample probe, and in that condition, press the start switch. Start switch Sysmex XP-300 7-15...
  • Page 82 Take care not to bend the sample probe. Note: The sample probe is automatically rinsed, so there is no need to wipe it clean. The analysis results for all parameters are displayed about 60 seconds after starting the analysis. 7-16 Sysmex XP-300...

  • Page 83: Analysis In Pre-Diluted (Pd) Mode

    All performance claims given in this manual were generated using specimens in EDTA anticoagulant. Results may be affected by the use of other anticoagulants. Therefore, each laboratory should develop protocols for handling specimens collected in their anticoagulants. Sysmex XP-300 7-17...
  • Page 84 When preparing a 1:26 dilution sample, use the tools listed below: • Diluent (CELLPACK) • Micro tube (MT-40, etc.) • Capillary tube • Probe: 500 μL • A container, such as erlenmeyer flask or beaker • A syringe 7-18 Sysmex XP-300...
  • Page 85 Also, sample ID “0” will not be incremented automatically. • A sample ID cannot be entered when the pneumatic unit operation is stopped. Sysmex XP-300 7-19...
  • Page 86 (2) Enter the sample ID. During the sample ID input, the most right character can be deleted by pressing the [C] button. (3) Press the [Ent.] button. The sample ID is defined, and the status changes to [Ready] for starting analysis. 7-20 Sysmex XP-300...
  • Page 87 ID after entry. For details about check digits, see “ID Bar code specifications” in “14.4 Connecting the bar code reader”. • Do not use any other characters than alpha- numerics, hyphens and space on the bar code label. Sysmex XP-300 7-21...
  • Page 88 (2) Enter the operator ID. During the operator ID input, the most right character can be deleted by pressing the [C] button. (3) Press the [Ent.] button. The dialog closes and the entered operator ID is registered. 7-22 Sysmex XP-300...
  • Page 89 ID after entry. For details about check digits, see “ID Bar code specifications” in “14.4 Connecting the bar code reader”. • Do not use any other characters than alpha- numerics, hyphens and space on the bar code label. Sysmex XP-300 7-23...
  • Page 90 (2) Select an operator ID. The operator ID is defined, and the status changes to [Ready] for starting analysis. If the operator ID you want to select is not displayed, press [more] to display all other operator IDs that are registered. 7-24 Sysmex XP-300...
  • Page 91 Vigorously mixing the sample can damage blood cells and cause bubbles to form in the sample. If this occurs, correct analysis results may not be obtained. (3) Remove the cap while taking care not to allow blood scatter. Sysmex XP-300 7-25...
  • Page 92 Take care not to bend the sample probe. Note: The sample probe is automatically rinsed, so there is no need to wipe it clean. The analysis results for all parameters are displayed about 60 seconds after starting the analysis. 7-26 Sysmex XP-300...

  • Page 93: Display Of Analysis Results

    For details, see “Output of analysis result (latest sample)” and “Output of stored data” in “8. Display and Output of Analysis Results”. Note: User settings can also be made for automatic output. (See “11. Instrument Setup”.) Sysmex XP-300 7-27...

  • Page 94: End Of Operation (Shutdown)

    (1) Press the [Shutdown] button at the Ready status. The shutdown confirmation dialog screen will appear. • In case the [Cancel] button is pressed, the Shutdown is canceled, and the instrument returns to the Main screen. 7-28 Sysmex XP-300...
  • Page 95 (3) Check that the shutdown sequence was completed and that the shutdown completion screen is displayed. Turn OFF the main power switch on the right side of the instrument. When the [Restart] button is pressed, the Main screen will appear. Sysmex XP-300 7-29...
  • Page 96 Sample Analysis 7-30 Sysmex XP-300...

  • Page 97: Display And Output Of Analysis Results

    The analysis result screen is composed of the first to fourth screens. Each screen can be switched by pressing the [←] or [→] button and displayed in the order as shown in the diagram below. First analysis result screen Second analysis result screen Fourth analysis result screen Third analysis result screen Sysmex XP-300...
  • Page 98 Displays analysis results of WBC, W-SCR, W-MCR, W-LCR, W-SCC, W-MCC and W-LCC parameters, or alternatively WBC, LYM%, MXD%, NEUT%, LYM#, MXD# and NEUT# parameters. Analysis mode Date and time of analysis Sample ID Operator ID Instrument error flag WBC histogram Analysis data Sysmex XP-300...
  • Page 99 Displays analysis results of WBC research parameters (ResearchW, ResearchS, ResearchM, ResearchL). Analysis mode Date and time of analysis Sample ID Operator ID Instrument error flag WBC histogram Analysis data * For details about WBC research parameters, see “2. WBC research parameters” in “14.6 Functional description”. Sysmex XP-300...
  • Page 100 At this time, the analysis error flag, [ERROR] (inverse in coloration display) appears. [---.-] Value could not be calculated due to data error, or volume distribution analysis parameter is not displayed when the analysis was performed in pre-diluted mode. Sysmex XP-300...
  • Page 101 The particle count equal to or less than WBC-LD has exceeded the range. For details about sample flagging, see “8.3 Histogram error flags”. WBC histogram: Displays a histogram for WBC. RBC histogram: Displays a histogram for RBC. PLT histogram: Displays a histogram for PLT. Sysmex XP-300...
  • Page 102 (2) Press the [WBC], [RBC] or [PLT] button to select a desired particle distribution for the manual discrimination. The manual discrimination screen for the selected particle distribution will appear. • Press the [Cancel] button to return to the analysis result screen. Sysmex XP-300...
  • Page 103 PLT particle distribution covers 40 channels of 0 to 39 (1 fL per channel), and the channel changes by one at a time. The relation between PLT particle distribution channel and fL display is as follows: Discri. position (fL) = (Channel No. + 1) × 1 Sysmex XP-300...
  • Page 104 • Press the [OK] button to update the changed contents and return to the analysis result screen. • Press the [Cancel] button to return to the manual discrimination screen. Analysis values changed in manual discrimination are reverse- displayed on the analysis result screen. Sysmex XP-300...
  • Page 105 The numeric keypad closes and the ID edit confirmation dialog will appear. • Press the [Set] button to define the sample ID and close the dialog. • Pressing the [Cancel] button closes the dialog without changing the sample ID. Sysmex XP-300...
  • Page 106 • When outputting to the host computer, it is possible to output to either the serial port (RS-232C) or LAN (Ethernet), depending on the user setting. • User settings can also be made for automatic output. (See “Host Output” and “Printer” in “11.2 Possible settings”.) 8-10 Sysmex XP-300...

  • Page 107: Stored Data

    • If the [Str. Data] button is pressed when no stored data are present, a dialog to the effect that no data are available is displayed instead of the list screen. Sysmex XP-300 8-11...
  • Page 108 [→] button and are displayed in the order as shown below. First list screen Second list screen Third list screen Fourth list screen Fourth detail screen Third detail screen Second detail screen First detail screen 8-12 Sysmex XP-300...
  • Page 109 Histogram error flag or numeric value abnormal flag Second list screen Displays the sample ID, analysis mode and operator ID in the stored data. Analysis mode Sample ID Operator ID Cursor sample Histogram error flag or numeric value abnormal flag Sysmex XP-300 8-13...
  • Page 110 Cursor sample Histogram error flag or numeric value abnormal flag Fourth list screen Displays the analysis data of HCT and PLT. Analysis mode Sample ID Analysis data Cursor sample Histogram error flag or numeric value abnormal flag 8-14 Sysmex XP-300...
  • Page 111 Displays the memory number of the data at the cursor position. Operator ID: Displays the operator ID of the analysis. Analysis data: Displays the analysis data of WBC, RBC, HGB, HCT and PLT. Instrument error flag: Appears when an instrument malfunction is detected during analysis. Sysmex XP-300 8-15...
  • Page 112 Displays the first list screen. The cursor is located at the sample data that is currently displayed. [↑]: Moves to the previous analysis result. However, when the oldest analysis result is displayed, this button operation becomes invalid. 8-16 Sysmex XP-300...
  • Page 113 • A bar code can also be input while displaying the numeric keypad. • The search dialog is displayed with the date on that day in the Date [Year], [Month] and [Day] display columns. Sysmex XP-300 8-17...
  • Page 114 • When outputting to the host computer, it is possible to output to either the serial port (RS-232C) or LAN (Ethernet), depending on the user setting. • User settings can also be made for automatic output. (See “Host Output” and “Printer” in “11.2 Possible settings”.) 8-18 Sysmex XP-300...
  • Page 115 • When outputting to the host computer, it is possible to output to either the serial port (RS-232C) or LAN (Ethernet), depending on the user setting. • User settings can also be made for automatic output. (See “Host Output” and “Printer” in “11.2 Possible settings”.) Sysmex XP-300 8-19...

  • Page 116: Deleting Stored Data

    The deletion confirmation dialog will appear. • Pressing the [OK] button deletes the analysis data selected or displayed. • Pressing the [Cancel] button in the output/delete dialog/ deletion confirmation dialog cancels the deletion and returns to the previous screen. 8-20 Sysmex XP-300...

  • Page 117: Histogram Error Flags

    1) Check smear, etc. incomplete lysing of red blood cells, etc. 2) Centrifuge sample and replace the plasma with equal volume of saline or CELLPACK and repeat analysis, warm sample at 37°C for 30 minutes and repeat analysis, etc. Sysmex XP-300 8-21...
  • Page 118 37°C for 30 minutes and repeat analysis, etc. [AG] Presence of nucleated red blood cells, effects of 1) Check smear, etc. fragmented red blood cells, increase of large platelets, platelet aggregation or agglutination, precipitation of fibrin, etc. 8-22 Sysmex XP-300...

  • Page 119: Quality Control

    EIGHTCHECK-3WP-H 1.5 mL × 12 vials 4.6 mL × 12 vials Intended purpose Control blood material is used to test the precision and accuracy of automated and semi-automatic hematology analyzers. Do not use EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H for the calibration of the system. Sysmex XP-300...

  • Page 120: Warning And Precautions

    12 hours at room temperature (25ºC). Additional special equipment EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H are intended to be used with SYSMEX reagents and analyzers only. Refer to the instrument manual for further information. Note: If other control materials are used the product performance of Sysmex instruments cannot be guaranteed.

  • Page 121: Manual Procedures

    Principle of the method EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H are to be used as hematology control bloods for the quality control of any Sysmex fully automated and semi- automated hematology analyzers. EIGHTCHECK-3WP-N is for the normal level, EIGHTCHECK-3WP-L is for the low abnormal level and EIGHTCHECK-3WP-H is for the high abnormal level.
  • Page 122 Methods. Ed.17. W.B. Saunders. Philadelphia, PA 1984. • Wintrobe, M.M. ‘Clinical Hematology’, 8th Edition, Lea and Febiger, Philadelphia, 1981. • Department of Labor, Occupational Safety and Health Administration. 29 CFR PART 1910. 1030: Occupational Exposure to Bloodborne Pathogens: Final Rule. Sysmex XP-300...

  • Page 123: Control Methods

    QC data than with the X control method so that the control thresholds are wider (or: have a higher range) compared to the X control method. An instrument’s performance over time can be monitored by conducting the L-J control 2 or more times during daily operation. Sysmex XP-300...

  • Page 124: Quality Control Process Flow

    Perform quality control analysis. Perform daily. Check and record quality control results (QC chart screen). Information A total of 6 quality control files can be stored. Each file can contain data of up to 22 parameters by 60 points. Sysmex XP-300...

  • Page 125: Control Method Selection

    The Settings menu screen will appear. Note: If a password has been set, the password input screen will appear. Enter the password. (3) Press the [Quality Control] button. A current control method will be displayed on the quality control setting screen. Sysmex XP-300...
  • Page 126 * In the quality control setting storing confirmation dialog, pressing the [Top] button will display the quit confirmation dialog. Press the [OK] button to return to the Main screen without changing the settings. Press the [Cancel] button to close the dialog to continue the settings. Sysmex XP-300...

  • Page 127: Settings For Control Blood Information (Qc Files)

    In a QC file, you can confirm and print the chart showing changes in the analysis results over time as shown below. + LIMIT TARGET - LIMIT This instrument stores the control blood information in 6 locations (QC files). Sysmex XP-300...
  • Page 128 Up to 10 digits can be input for the expiration date. Note: • The analyzer does not judge the expiration dates entered in the QC file. • Only [yyyy/mm/dd] is available for the expiration date input format. 9-10 Sysmex XP-300...
  • Page 129 When 2 or more plots are present in the base QC data, the TARGET value and LIMIT value can be calculated automatically. For details, see “Automatic settings of the TARGET value and LIMIT value” in “9.5 Settings for control blood information (QC files)”. Sysmex XP-300 9-11...
  • Page 130 • Pressing the [IP] button on the first QC file setting screen prints out the current file’s lot ID, expiration date, TARGET value and LIMIT width of each parameter in the quality control printing format. 9-12 Sysmex XP-300...
  • Page 131 QC file setting screen to the second screen. Pressing the [→] button on the sixth screen returns to the first screen. When the [←] button is pressed, the display changes in the reverse order. Note: The QC file setting screens are consisted of 6 screens. Sysmex XP-300 9-13...
  • Page 132 • Pressing the [IP] button on the first QC file setting screen prints out the current file’s lot ID, expiration date, TARGET value and LIMIT width of each parameter in the quality control printing format. 9-14 Sysmex XP-300...
  • Page 133 [2SD] and [3SD]. 2SD or 3SD of all plots is automatically calculated, and displayed on the button in the [LIMIT] column. If 1 or no plot presents: A dialog to the effect that no calculation is possible is displayed. Sysmex XP-300 9-15...

  • Page 134: Performing A Quality Control

    (QC files)”.) Note: Pressing the [IP Print] button on the analysis starting screen prints out the current file’s lot ID, expiration date, TARGET value and LIMIT width of each parameter in the quality control printing format. 9-16 Sysmex XP-300...
  • Page 135 If it is used after this point, abnormal count values may result. (3) Remove the cap while taking care not to allow blood to scatter. (4) Set the control blood container to the sample probe and press Start switch the start switch in that status. Sysmex XP-300 9-17...
  • Page 136 [NG] button. In either case, remove the control blood, close the cap, and mix well. Then, remove the cap again, set the control blood to the sample probe, and press the start switch of the instrument. 9-18 Sysmex XP-300...
  • Page 137 X2 analysis results: QC03-2 Mean of X1 and X2: QC03 (The mean does not contain histogram data.) From the stored data screen for output, the result data can be printed out or transferred to the host computer. Sysmex XP-300 9-19...
  • Page 138 Press the button and the action message will appear. Press the [Back] button to turn off the alarm. Information For details about actions to resolve the QC (X) error, see “QC(X-bar) Error” in “13. Troubleshooting”. 9-20 Sysmex XP-300...
  • Page 139 If it is used after this point, abnormal count values may result. (3) Remove the cap while taking care not to allow blood to scatter. (4) Set the control blood container to the sample probe and press Start switch the start switch in that status. Sysmex XP-300 9-21...
  • Page 140 Use the [←] or [→] button to scroll the screen pages. • To print results on the internal printer, press the [IP] button. Information The print format is used for quality control analysis only and cannot be changed. 9-22 Sysmex XP-300...
  • Page 141 Then an alarm sounds and the QC error message is displayed. Press the button and the action message will appear. Press the [Back] button to turn off the alarm. Information For details about actions to resolve the QC (L-J) error, see “QC(L-J) Error” in “13. Troubleshooting”. Sysmex XP-300 9-23...

  • Page 142: Quality Control Chart Screen

    The Menu screen will appear. (2) Press the [QC Chart] button. The quality control chart screen (1/11) will appear. Note: When the quality control chart screen is displayed, the screen for the sample most recently viewed or updated appears. 9-24 Sysmex XP-300...
  • Page 143 PDW, MPV Fifth quality control chart screen LYM%, MXD% Eleventh quality control chart screen P-LCR, PCT Sixth quality control chart screen NEUT%, LYM# Lot ID File Expiration date Date and time of analysis Item Chart Operator ID Sysmex XP-300 9-25...
  • Page 144 If the operator calls up another parameter by using the [↑] or [↓] button, the same range of the data are displayed for that parameter. Operator ID: Displays the operator ID of the analysis. 9-26 Sysmex XP-300...

  • Page 145: External Output

    • User settings can also be made for automatic output. (See “Host Output” in “11.2 Possible settings”.) • When no setting is made to output to the host computer, the [Current] button next to “HC” becomes invalid. Sysmex XP-300 9-27...

  • Page 146: Deleting Data

    • Press the [OK] button to delete the data at the cursor position in the quality control chart and close the dialog. • Press the [Cancel] button to cancel control data deletion and return to the previous screen. 9-28 Sysmex XP-300...

  • Page 147: Calibration

    This is very important in maintaining the system accuracy. Calibration is carried out by entering calibration values into the instrument. Your Sysmex service representative will perform initial calibration of your unit at installation. After installation, the operator should make periodical calibration and proper quality control to maintain the accuracy.

  • Page 148: Calibration Flow Chart

    • Calculate calibration value. Page 10-9 Perform analysis Page 10-7 (* in whole blood mode). Update calibration value. • Select manual calibration mode. • Enter calibration value. Update calibration value. Page 10-8 Page 10-10 Completion of HGB/HCT calibration 10-2 Sysmex XP-300...

  • Page 149: Samples Used For Calibration

    HCT should be accurately analyzed 3 times each in accordance with the following reference methods. The measurements thus obtained are used as reference values. HGB values: Cyanmethemoglobin method HCT values: Microhematocrit method (5 samples are used for automatic calibration.) Sysmex XP-300 10-3...

  • Page 150: Automatic Calibration

    (1) Press the [Menu] button at the Ready status. The Menu screen will appear. (2) Press the [Calib.] button. The calibrating menu screen will appear. Note: If a password has been set, the password input screen will appear. Enter the password. 10-4 Sysmex XP-300...
  • Page 151 Calibration (3) Press the [Auto Calib.] button. The automatic calibration target value setting screen will appear. Sysmex XP-300 10-5...
  • Page 152 * Press the [Top] button to display the setting cancel confirmation dialog. Press the [OK] button to return to the Main screen without changing any setting, and the [Cancel] button to close the dialog and then to continue the setting. 10-6 Sysmex XP-300...
  • Page 153 Remove the sample tube by that time. • To remove the sample tube, lower it straight down. Take care not to bend the sample probe. Note: The sample probe is automatically rinsed, so there is no need to wipe it clean. Sysmex XP-300 10-7...
  • Page 154 The calibration value change confirmation message will appear. • Press the [OK] button to update the calibration values, and to return to the Main screen. • Press the [Cancel] button to return to the Main screen without updating the calibration values. 10-8 Sysmex XP-300...

  • Page 155: Manual Calibration

    Average of HGB values gained by this instrument = 14.2 g/dL Previous calibration value of HGB = 98.7% 14.0 98.7 × = 97.30 97.3 14.2 Therefore, the new calibration value of HGB needs to be set at 97.3%. Sysmex XP-300 10-9...
  • Page 156 If a password has been set, the password input screen will appear. Enter the password. (3) Press the [Manual Calib.] button. The manual calibration setting screen will appear. (4) Press the calibration value display column for the calibrated parameter. The numerical keys dialog will be displayed. 10-10 Sysmex XP-300...
  • Page 157 × 100 Current calibration value • Press the [OK] button to update the calibration values, and to return to the Main screen. • Press the [Cancel] button to return to the Main screen without updating the calibration values. Sysmex XP-300 10-11...

  • Page 158: Printing Calibration History

    The calibrating menu screen will appear. Note: If a password has been set, the password input screen will appear. Enter the password. (3) Press the [Print Cal. His.] button. The internal printer will print the calibration history. 10-12 Sysmex XP-300...

  • Page 159: Instrument Setup

    The screen for the selected parameter will appear. When the [←] and [→] buttons are displayed on the bottom of the screen, the setting contents are divided into 2 or more pages. Pages can be switched by pressing the [←] or [→] button. Sysmex XP-300 11-1...
  • Page 160 * Press the [Top] button to display the setting cancel confirmation dialog. Press the [OK] button to return to the Main screen without changing any setting, and the [Cancel] button to close the dialog and then to continue the setting. 11-2 Sysmex XP-300...

  • Page 161: Possible Settings

    [Type 1]/[Type 2]/[Type 3]/[Type 4]/[Type 5]/ [Type 6] [Type 1]: High continuous beep [Type 2]: Repeated high beeps [Type 3]: Repeated high 2-tone beeps [Type 4]: Low continuous beep [Type 5]: Repeated low beeps [Type 6]: Repeated low two-tone beeps Sysmex XP-300 11-3...
  • Page 162 Numeric input: 2000 - 2037 [Month] Numeric input: 1 - 12 [Day] Numeric input: 1 - 28/29/30/31 Determine the available numeric input range by the Year and Month settings. [Hour] Numeric input: 0 - 23 [Minute] Numeric input: 0 - 59 11-4 Sysmex XP-300...
  • Page 163 If LL or UL that satify the following conditions was entered in the Patient Limit setting screen, a beep sounds and the entering is canceled. • LL is higher than UL. • UL is lower than LL. Sysmex XP-300 11-5...
  • Page 164 [ID Pad.] [0 Pad.]/[SpacePad.] [RDW] [RDW-SD]/[RDW-CV] [ASTM Rev.] [1381-95]/[1381-02] Note: The [RDW] setting is only available when [K-1000] is selected in [Format]. Data will be output in both parameters, RDW-SD and RDW-CV, for any other options in [Format]. 11-6 Sysmex XP-300...
  • Page 165 Numeric input: 0 - 9999 Default: “0” *1: Common parameters to the internal/external printer *2: Parameters for the internal printer *3: Parameters for the external printer *4: Only available when [Bitmap (for PC)] is selected in [Type]. Sysmex XP-300 11-7...
  • Page 166 0 - 255 (common to all columns) Default: “192.168.0.20” [Port Number] Numeric input: 0 - 9999 Default: “5006” Note: Numeric input exceeding the upper limit is replaced with the upper limit value in any parameters other than [Port Number]. 11-8 Sysmex XP-300...

  • Page 167: Password Setting

    Key in the password and confirm with the [Ent.] button. Information In case the password has been forgotten, contact your local Sysmex service representative. Password setting procedure (1) Press the [Menu] button on the Main screen. The Menu screen will appear.
  • Page 168 * Press the [Top] button on the setting screen. The setting stop confirmation dialog will appear. Press the [OK] button to return to the Main screen without changing the settings. Press the [Cancel] button to close the dialog and continue the setting. 11-10 Sysmex XP-300...

  • Page 169: Print Settings

    (4) Press the desired output destination button. • [IP]: Prints the set values on the internal printer. • [GP]: Prints the set values on the graphic printer. * Press the [Top] button to close the print set value dialog. Sysmex XP-300 11-11...
  • Page 170 Instrument Setup 11-12 Sysmex XP-300...

  • Page 171: Cleaning And Maintenance

    • Clean rinse cup (see 12.10) • Dispose waste fluid (see 12.11) • Clean aperture of TD chamber (see 12.12) • Calibrate LCD screen (see 12.13) • Reset SRV cycle counter (see 12.14) • Adjust pressure and vacuum (see 12.15) Sysmex XP-300 12-1...

  • Page 172: Check Instrument Status

    Cleaning and Maintenance 12.2 Check instrument status If you should contact the Sysmex service representative regarding any instrument problem, you may be asked to check the operation counter, program version, and other information. (1) Press the [Menu] button at the Ready status.

  • Page 173: Clean Td Chambers And Diluted Sample Lines (Shutdown)

    • It takes approx. 5 minutes to complete the Shutdown sequence. (1) Press the [Shutdown] button at the Ready status. The Shutdown confirmation message will appear. In case the [Cancel] button is pressed, the Shutdown is canceled, and the instrument returns to the Main screen. Sysmex XP-300 12-3...
  • Page 174 • To remove the CELLCLEAN container, lower it straight down. Take care not to bend the sample probe. (3) Check that the Shutdown completion screen appears. Turn OFF the main power switch on the right side of the instrument. 12-4 Sysmex XP-300...

  • Page 175: Check Trap Chamber Level And Discard

    Caution! • If liquid collects everyday, the hydraulic system may have failed. Contact your Sysmex technical representative. • Pay attention to the direction of the float in the chamber. Place it with its pointed end facing upward.

  • Page 176: Clean Srv Tray

    If analysis is made with the screw loosened, air can enter the system and affect the data. Information Mount the SRV tray properly as to the face/bottom and the direction. (7) Close the front cover of the main unit. 12-6 Sysmex XP-300...

  • Page 177: Clean Td

    Risk of Infection When opening the transducer cover, wear protective garments and gloves. After completion of work, wash hands with disinfectant. If your hands are contaminated by blood, etc., there is a risk of infection by pathogens. Sysmex XP-300 12-7...
  • Page 178 Then press the start switch. After completion of TD cleaning, the Main screen will appear. Information When the cleaning is performed, the detector counter (number of cycles since last cleaning of TD) is automatically reset. 12-8 Sysmex XP-300...

  • Page 179: Clean Waste Chamber

    If the skin or clothes should come in touch with it, flush it away using plenty of water to avoid injury or damage. Otherwise, it can damage the skin or clothes. Sysmex XP-300 12-9...
  • Page 180 Then, Auto Rinse and background check will be performed. (2) After completion of waste chamber cleaning, the Main screen will appear. Information When the cleaning is performed, the detector counter (number of cycles since last cleaning of waste chamber) is automatically reset. 12-10 Sysmex XP-300...

  • Page 181: Clean Srv

    When cleaning the SRV before the above message is displayed, reset the SRV counter (see “12.14 Reset SRV cycle counter”). (2) Turn off the power of the main unit and wait approximately 30 seconds. (3) Open the front cover of the main unit. Sysmex XP-300 12-11...
  • Page 182 • Take care not to pull out the SRV excessively. This is to prevent applying excess force to the tube connected to the SRV. • When removing the SRV, take care not to bend the sample probe. 12-12 Sysmex XP-300...
  • Page 183 (11) Make sure the valve contact surfaces are completely free from dirt or dust. Caution! If the device is used with dirt or dust attaching on valve contact surfaces, blood leakage can occur and correct analysis results may not be obtained. Sysmex XP-300 12-13...
  • Page 184 (14) Close the front cover of the main unit. (15) Turn on the main unit and make sure that the blank error has not occurred. (16) Perform quality control and make sure that there is no functional problem. 12-14 Sysmex XP-300...

  • Page 185: Perform Auto Rinse

    The background check is performed up to 3 times and, if in all three background checks one parameter exceeds the limits, the message [Blank Error] is displayed on the screen and an alarm is sounded. Information See “Blank Error” in “13. Troubleshooting” to deal with Blank error. Sysmex XP-300 12-15...
  • Page 186 Cleaning and Maintenance (5) When the Auto Rinse and Background check are normally completed, the Main screen will appear. Press the [Result] button and Background check result will appear. 12-16 Sysmex XP-300...

  • Page 187: Clean Rinse Cup

    (4) Remove the rinse cup in the order of (1), (2), and (3) as shown on the left. Rinse cup (5) Clean the rinse cup using tap water. (6) Make sure no contaminant remains on the rinse cup and wipe off water. Sysmex XP-300 12-17...
  • Page 188 Make sure the rinse cup is pushed all the way up with the sample probe inserted in the hole. If the power is turned on with the rinse cup staying lowered, [Rinse motor error] will occur, making it impossible to continue the operation. 12-18 Sysmex XP-300...

  • Page 189: Dispose Waste Fluid

    • If the waste fluid is spilt, wipe off with the damp cloth immediately. • When using a used reagent container as the waste container, make sure to clearly mark it that it is the waste container. Sysmex XP-300 12-19...

  • Page 190: Clean Aperture Of Td Chamber (Drain Td Chamber)

    (1) Press the [Menu] button at the Ready status. The Menu screen will appear. (2) Press the [Maint.] button. The maintenance menu screen will appear. (3) Press the [Drain TD Chamber] button. The drain TD chamber screen will appear. 12-20 Sysmex XP-300...
  • Page 191 (6) Open the front cover of the main unit. (7) Open the transducer cover. Loosen Warning! When the power is on, never open the transducer cover. This is to prevent possible electric shocks. Transducer cover Sysmex XP-300 12-21...
  • Page 192 The TD chamber plug should be mounted securely. Otherwise, current leakage or electric shocks can occur. (12) Close the transducer cover and main unit front cover. Then turn on the power. (13) Check that the blank error has not occurred. 12-22 Sysmex XP-300...

  • Page 193: Calibrate Lcd Screen

    The “+” mark will appear on the LCD in the following order: at the center, the upper left, the lower left, the lower right, and the upper right. After completion of all “+” marks input, the calibration LCD confirmation dialog screen will appear. Sysmex XP-300 12-23...
  • Page 194 • When calibration is performed and a calibration error occurs frequently, there may be a problem with the touch panel. Contact your Sysmex service representative for further assistance. 12-24 Sysmex XP-300...

  • Page 195: Reset Srv Cycle Counter

    (1) Press the [Menu] button at the Ready status. The Menu screen will appear. (2) Press the [Maint.] button. The maintenance menu screen will appear. (3) Press the [SRV reset] button. The SRV reset dialog will appear. Sysmex XP-300 12-25...

  • Page 196: Adjust Pressure And Vacuum

    12.15 Adjust pressure and vacuum The adjustment procedures described here are important in maintaining accuracy of this instrument. Your Sysmex service representative will perform initial adjustment. Should it require any adjustment subsequently, follow the procedures given below. The following adjustment procedures are explained on the following pages.
  • Page 197 Cleaning and Maintenance Location of control knobs Bellows unit Regulator Note: When an error has occurred, press in the error dialog to check pressure or vacuum value on the action message screen. (See “13. Troubleshooting”) Sysmex XP-300 12-27...
  • Page 198 (4) When adjustment is finished, tighten the locking nut while taking care not to allow the adjusting knob to rotate. (5) Press the [Top] button to close the status display screen. 12-28 Sysmex XP-300...
  • Page 199 (4) When adjustment is finished, tighten the locking nut while taking care not to allow the adjusting knob to rotate. (5) Press the [Top] button to close the status display screen. Sysmex XP-300 12-29...

  • Page 200: Replace Reagent

    • Take care to prevent dust, dirt, bacteria, or other materials from entering the reagent after it is opened. Otherwise, there is a possibility that proper analysis cannot be performed. Shelf life after first opening CELLPACK 60 days STROMATOLYSER-WH 90 days 12-30 Sysmex XP-300...
  • Page 201 (5) Press the [Menu] button on the Main screen. To execute from the error help screen, press the [Execute] button. (proceed to (8).) (6) Press the [Chg.Reag] button. The reagent information screen will appear. Sysmex XP-300 12-31...
  • Page 202 * Pressing the [Top] button on the reagent bar code entering screen displays the replace reagent quit conformation dialog. Press the [OK] button to return to the Main screen without replacing the reagent or the [Cancel] button to close the dialog to continue the reagent replacement operation. 12-32 Sysmex XP-300...
  • Page 203 Up to 100 data can be confirmed in the reagent replacement log. Information If the data records exceed 100, the oldest data is automatically deleted. (1) Press the [Reag. Log] on the reagent information screen. The reagent replacement history screen will appear. Sysmex XP-300 12-33...
  • Page 204 [IP]: Prints 10 replacement logs that are displayed on the screen. This button dims while printing. [↑]: Moves to the previous log data. However, when the oldest data is displayed, this button operation becomes invalid. 12-34 Sysmex XP-300...

  • Page 205: Replace System Fuses

    Fuse cap holder This is to prevent possible smoke. Notch Specification Part No. Description Fuse Type 120 VAC 266-5011-3 Fuse 250V 4A Time Lag ST4-4A-N1 230/240 VAC AX880901 Fuse 250V 3.15A Time Lag 50T032H Sysmex XP-300 12-35...

  • Page 206: Replace Internal Printer Paper (Printing Paper No. 3)

    (3) Remove the bare paper core and mount the new printer paper. Printer cover Note: When any printer paper remains, remove it together with the paper core. New printer paper (Printing Paper No. 3) (4) Pass the printer paper through along the paper guides. Paper guide 12-36 Sysmex XP-300...
  • Page 207 The printer head becomes hot. Use caution. Caution! Static electricity may damage the printer head. Do not touch with hands. (6) Cut off any printer paper extending from the upper part of the printer. (7) Close the front cover of the main unit. Sysmex XP-300 12-37...

  • Page 208: List Of Consumables

    50 mL Detergent (CELLCLEAN) Note: A 20 L or 10 L Cubitainer spout kit is required when you use CELLPACK. For details, contact your Sysmex service representative. 943-1781-1 Cubitainer Spout Kit No. 1 (for 20 L) 943-1782-4 Cubitainer Spout Kit No. 1 (for 10 L) Consumable list Part No.

  • Page 209: Troubleshooting

    If the corresponding item is not found, or if the procedure listed under “Action” does not eliminate the signs of trouble, contact your Sysmex service representative. • Other errors are indicated by a beep and a message is then displayed on the LCD screen.

  • Page 210: When You Suspect An Error

    Then turn the main power switch ON again. If the error persists, contact your Sysmex service representative. • Check if power cord is plugged in properly. The instrument is switched ON but will not start.

  • Page 211: Error Messages

    The help screen of the error at the top of the list will be displayed. • Follow the instructions. • Press the [Detail] button to go to the next screen. • Press the [Back] button to close the screen. • If the error persists, contact your Sysmex service representative. Sysmex XP-300 13-3...
  • Page 212 WBC Smp’g Error ........13-13 13-4 Sysmex XP-300...
  • Page 213 RBC Analysis Err........13-14 Sysmex XP-300...
  • Page 214 SNCS Status Error ........13-21 13-6 Sysmex XP-300...

  • Page 215: Error Messages, Possible Causes And Actions To Revolve The Error

    13.3 Error messages, possible causes and actions to revolve the error Note: An error subcode may be displayed after the error code. The subcode is for Sysmex service representative use only. 1. Pressure/Vacuum Errors Error Message 0.05MPa Pressure Error (113005)
  • Page 216 • Not ready until the error is resolved. Note • The status is not ready for analysis, but the processing, etc. of stored data can be performed. 13-8 Sysmex XP-300...
  • Page 217 However, an error occurs again if the reagent replacement sequence is not completed correctly. • Not ready until the error is resolved. Note • The status is not ready for analysis, but the processing, etc. of stored data can be performed. Sysmex XP-300 13-9...
  • Page 218 Replace with a new reagent and press the [Execute] button. on the screen The error is cleared and the reagent replacement sequence is executed. Refer to “12.16 Replace reagent”. However, an error occurs again if the reagent replacement sequence is not completed correctly. 13-10 Sysmex XP-300...
  • Page 219 Press the [Execute] button to clear the error, and the clog on the screen removal is executed. • The analysis result with an error is masked on the screen. Note • Although the system is ready for analysis, the error may occur in the next analysis. Sysmex XP-300 13-11...

  • Page 220: Temperature Errors

    PLT background count. Troubleshooting Press the [Execute] button to clear the error, and the auto on the screen rinse program is executed. Note Ready for analysis (although the next sample could be affected) 13-12 Sysmex XP-300...
  • Page 221 Separate the source of electric noise from the instrument. • The analysis result with an error is masked on the screen. Note • Although the system is ready for analysis, the error may occur in the next analysis. Sysmex XP-300 13-13...
  • Page 222 (CELLPACK). Action Replenish diluent (CELLPACK). • The analysis result with an error is masked on the screen. Note • Although the system is ready for analysis, the error may occur in the next analysis. 13-14 Sysmex XP-300...

  • Page 223: Memory Errors

    • When the instrument is initialized, all stored data is deleted. If settings have been initialized, reenter the settings. • If either “repair” or “initialize” is successful, the program will be started. Note Analysis is not ready. Not ready until the error is resolved. Sysmex XP-300 13-15...
  • Page 224 Check QC data, and if data is found to have shifted, a hardware problem is conceivable. Referring to “12. Cleaning and Maintenance”, clean the TD, SRV, etc. Note Analysis is not ready. Not ready until calibration value is fixed. 13-16 Sysmex XP-300...

  • Page 225: Maintenance Errors

    * The same message will appear the next time you start the unit; it will be displayed at each startup until the cleaning is performed. Note When the TD rinse sequence has been completed, the cycle counter is automatically reset. Sysmex XP-300 13-17...
  • Page 226 The error is cleared and the data under printing will be printed. • Press the [Cancel] button to cancel the data under printing, also discard the waiting data, and the error is cleared. Note Analysis is ready when the error is cleared. 13-18 Sysmex XP-300...
  • Page 227 The amount of data for output or printout is too large to be processed. Action Troubleshooting Press the [Back] button to clear the error. on the screen However, the data under printing will be printed. Other data will be deleted. Note Analysis is ready when the error is cleared. Sysmex XP-300 13-19...
  • Page 228 • Press the [Cancel] button to cancel the data under printing, also discard the waiting data, and the error is cleared. Note Analysis is ready when the error is cleared. 13-20 Sysmex XP-300...
  • Page 229 • Check if the communications equipment power is turned ON Action • Check if the connection cable of the communications equipment is connected properly. • Contact your local Sysmex service representative. • Press the [Retry] button to clear the error and the Troubleshooting on the screen transmission to the SNCS server will resume.

  • Page 230: Print Error Log

    The maintenance menu screen will appear. (3) Select the [Print Error Log] button. The internal printer will print out the error history. Note: An error subcode may be printed after the error code. The subcode is for Sysmex service representative use only. 13-22 Sysmex XP-300...

  • Page 231: Technical Information

    10.0 - 999 × 10 /µL Background limits 0.3 × 10 /µL or less 0.02 × 10 /µL or less 0.1 g/dL or less 10 × 10 /µL or less Reagent Diluent: CELLPACK WBC/HGB lyse reagent: STROMATOLYSER-WH Detergent CELLCLEAN Sysmex XP-300 14-1...
  • Page 232 Whole blood mode: Approx. 50 µL aspirated Pre-diluted mode: Approx. 200 µL (Diluted blood sample) Blood volume required for the pre-dilution is 20 µL or over. Analysis principle WBC: DC detection method RBC/PLT: DC detection method HGB: Non-cyanide hemoglobin analysis method 14-2 Sysmex XP-300...
  • Page 233 15.0% or less MXD# 30.0% or less (1.0 × 10 /µL or more) NEUT# 15.0% or less RDW-CV 4.0% or less RDW-SD 4.0% or less 12.0% or less 5.0% or less P-LCR 20.0% or less 9.0% or less Sysmex XP-300 14-3...
  • Page 234 Mean values when calibrator is analyzed more than 10 times continuously are Pre-diluted mode within the following ranges against the displayed values. ±5% or ±0.3 × 10 /µL ±3% or ±0.05 × 10 /µL ±8% or ±15 × 10 /µL 14-4 Sysmex XP-300...
  • Page 235 IEC 61326-1: 97+A1: 98 (EN 61326: 97+A1) Electrical equipment for compatibility) measurement, control and laboratory use -EMC requirements- - EMI (Class A) - EMS (Minimum Immunity test requirements) EMC Conformity IEC61326-2-6:2005 Assessment EMI:ClassA Standards Safety IEC61010-1: 2001, IEC61010-2-081: 2001+A1, IEC61010-2-101: 2002 Sysmex XP-300 14-5...
  • Page 236 Socket for bar code reader : ±25V Serial : ±25V Socket for graphic printer : -0.5V - 7V Socket for LAN (SNCS service) : -0.3V - 6V 2) Rated insulation: Devices with certified safety must be connected. 14-6 Sysmex XP-300...

  • Page 237: System Limits

    If any of the following is present, the system may erroneously report a high red blood cell count. • Leukocytosis (> 100,000/µL) • Giant platelets (platelets > 1,000,000/µL) If any of the following is present, the system may erroneously report a high haemoglobin concentra- tion. • Leukocytosis (> 100,000/µL) • Lipemia • Abnormal protein Sysmex XP-300 14-7...
  • Page 238 • Possibility of PLT clumps • Pseudothrombocytopenia • Giant platelets If any of the following is present, the system may erroneously report a high platelet count. • Microerythrocytes • Possibility of fragmented RBCs • Fragmented leukocytes • Cryoprotein • Cryoglobulin 14-8 Sysmex XP-300...

  • Page 239: Print Formats

    Technical Information 14.3 Print formats Shown are examples of printed results on the internal printer. For the setting procedures, see “11.2 Possible settings”. Output on internal printer (IP) Type 1 Type 2 Type 3 Sysmex XP-300 14-9...
  • Page 240 Technical Information Examples of printed results on the graphic printer (GP) 14-10 Sysmex XP-300...
  • Page 241 Technical Information Examples of printed results on the list printer (LP) Note: A numeric value abnormal flag is printed to the right of each value on the list printer (LP). Sysmex XP-300 14-11...

  • Page 242: Connecting The Bar Code Reader

    (2) Insert the other end of the connection cable into the bar code reader connector port at the back of the instrument. Bar code reader connector port (3) Turn the main power switch ON. Press the trigger switch and check that the red LED is ON. Trigger switch 14-12 Sysmex XP-300...

  • Page 243: Hardware Specifications

    Reader → Analyzer RTS (Request To Send) Reader ← Analyzer CTS (Clear To Send) NC (Not Connected) Reader ← Analyzer DTR (Data Terminal Ready) SG (0 V) Reader – Analyzer Reader ← Analyzer +5 V (+5 V) Sysmex XP-300 14-13...

  • Page 244: Software Specifications

    Parameter Setting value Baud rate 9600 bps Data length 8 bits Stop bit 1 bit Parity None RTS/CTS Valid Transfer sequence No protocol Check digit Not transmit ID character Not transmit Preamble STX (02H) Postamble ETX (03H) 14-14 Sysmex XP-300...
  • Page 245 The actual specifications may differ depending on the symbology, print quality, and number of digits. Specifications for JAN codes will also be somewhat different. For details, contact your Sysmex service representative. Symbologies used The symbologies and check digits which can be used are listed below.
  • Page 246 Bar code label dimensions Bar height Space Effective bar code width Space Space: Min. 2.5 mm or 10 × largest module value Effective bar code width: Max. 48 mm (Optimum: 40 mm or less) Bar height: Min. 20 mm 14-16 Sysmex XP-300...
  • Page 247 Second set: × × × × × × Weight 2) The results of multiplication are all added together. The result is S. S = 16 + 20 + 40 + 12 + 6 + 12 = 106 Sysmex XP-300 14-17...
  • Page 248 0. If the remainder is 0 when S is divided by 11, or if the result of check-digit calculation is 0, then the check digit is 0. Note: The weight of digits 13 to 15 is 0. 14-18 Sysmex XP-300...

  • Page 249: System Of Units

    × 10 RDW-SD ###.# ###.# ###.# RDW-CV ###.# ###.# #.### ###.# ###.# ###.# ###.# ###.# ###.# P-LCR ###.# ###.# #.### ##.## ##.## #.#### ResearchW ####.## × 10 /μL ###.### × 10 /μL ###.### × 10 ResearchS ResearchM ResearchL Sysmex XP-300 14-19...
  • Page 250 ###.# × 10 RDW-SD ###.# ###.# ###.# RDW-CV #.### #.### ###.# ###.# ###.# ###.# ###.# ###.# ###.# P-LCR #.### #.### ###.# #.#### #.#### ##.## ResearchW ###.### × 10 ###.### × 10 ###.### × 10 ResearchS ResearchM ResearchL 14-20 Sysmex XP-300...

  • Page 251: Functional Description

    Blood cell count is calculated by counting the pulses, and a histogram of blood cell sizes is plotted by determining the pulse sizes. Also, analyzing a histogram makes it possible to obtain various analysis data. DC Supply TD Chamber Resistance External Electrode + Blood Cell Suspension Aperture Internal Electrode - Sysmex XP-300 14-21...
  • Page 252 Oxyhemoglobin method and contains no poisonous substance, making it suitable for automated method. Being capable of analyzing methemoglobin, this method can accurately analyze control blood, etc. which contain methemoglobin. 14-22 Sysmex XP-300...
  • Page 253 Whole Blood Sample (50 µL) <Pre-diluted Mode> 1.99792 mL Diluent RBC TD Chamber 2.08 µL (1:25,000) 1.922 mL 1.0 mL WBC TD Chamber WBC/HGB Lyse 78 µL (1:1,000) HGB Flow Cell (1:1,000) 1:26 Diluted Sample (200 µL) Sysmex XP-300 14-23...
  • Page 254 HGB flow cell. Concentration of this sample is measured as absorbance. This absorbance is compared with that of the diluent alone that was measured before addition of the sample, thereby calculating HGB (hemoglobin value). 14-24 Sysmex XP-300...
  • Page 255 HGB flow cell. Concentration of this sample is measured as absorbance. This absorbance is compared with that of the diluent alone that was measured before addition of the sample, thereby calculating HGB (hemoglobin value). Sysmex XP-300 14-25...
  • Page 256 (4) 250 µL of the sample in the RBC/PLT TD chamber is aspirated through the aperture. At this time, RBC and PLT are counted by the DC detection method. At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection method. 14-26 Sysmex XP-300...
  • Page 257 (3) Of the sample in the RBC/PLT TD chamber, 250 µL is aspirated through the aperture. At this time, RBC and PLT are calculated by the DC detection method. At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection method. Sysmex XP-300 14-27...
  • Page 258 Calculation is made from RBC and HGB by the formula below: HGB (g/dL) MCH (pg) = ×10 RBC (×10 /µL) 3. Mean RBC Hemoglobin Concentration (MCHC) Calculation is made from HCT and HGB by the formula below: HGB (g/dL) MCHC (g/dL) = ×100 HCT (%) 14-28 Sysmex XP-300...
  • Page 259 As to PLT LOWER discriminator and UPPER discriminator, the optimum position in 2 - 6 fL and 12 - 30 fL, respectively, are automatically determined by the microcomputer. PLT count is calculated from the particle counts between this LOWER discriminator and UPPER discriminator. Sysmex XP-300 14-29...
  • Page 260 NEUT# [W-LCC (WBC-Large Cell Count)] Neutrophils more than discriminator (T2), which is considered highly correlated with neutrophils. LYM% [W-SCR (WBC-Small Cell Ratio)] Ratio of lymphocytes to whole WBC MXD% [W-MCR (WBC-Middle Cell Ratio)] Ratio of mixed cells to whole WBC 14-30 Sysmex XP-300...
  • Page 261 • Interface protocol ResearchS, ResearchM, ResearchL: Can be output to the host computer in XP format. ResearchW: Can be output to the host computer in XP format or pocH format. For details, contact your local Sysmex service representative. Sysmex XP-300 14-31...
  • Page 262 AG: The particle count equal to or less than the LD exceeds a prescribed range. Probable cause is platelet agglutination, which does not alter WBC count but may result in decreased platelet count. Therefore, this flag is added to the PLT parameter. 14-32 Sysmex XP-300...
  • Page 263 WL flag is added to all WBC parameters (WBC, LYM%, MXD%, NEUT%, LYM#, MXD#, NEUT#). • WBC Histogram Error-WL (1A) LD T1 8.5 [×10 /µL] LYM% 39.3 [%] MXD% 17.6 [%] NEUT% 43.1 [%] LYM# 3.4 [×10 /µL] MXD# 1.5 [×10 /µL] [fL] NEUT# 3.6 [×10 /µL] Sysmex XP-300 14-33...
  • Page 264 This WBC value exceeds the upper Patient Mark Limits mentioned earlier. • WBC Histogram Error-WL (1C) WL +16.4 [×10 /µL] LYM% ---.- [%] MXD% ---.- [%] NEUT% ---.- [%] LYM# ---.- [×10 /µL] MXD# ---.- [×10 /µL] [fL] NEUT# ---.- [×10 /µL] 14-34 Sysmex XP-300...
  • Page 265 3B Histogram with high T1 but without T2, and high in UD frequency WBC is flagged with WU and lymphocyte parameters (LYM%, LYM#) are flagged with F1. Mixed cell and neutrophil parameters (MXD%, NEUT%, MXD#, NEUT#) are flagged with T2 and their data are not output. Sysmex XP-300 14-35...
  • Page 266 /µL] 11) 4B Histogram with high T1 and UD WBC is flagged with WU, lymphocyte parameters (LYM%, LYM#) are flagged with F1, and mixed cell parameters (MXD%, MXD#) with F2. Neutrophil parameters (NEUT%, NEUT#) are not flagged. 14-36 Sysmex XP-300...
  • Page 267 15) 5B Histogram with high T2 and high UD WBC is flagged with WU and lymphocyte parameters (LYM%, LYM#) are not flagged. Mixed cell parameters (MXD%, MXD#) are flagged with F2, and neutrophil parameters (NEUT%, NEUT#) with F3. Sysmex XP-300 14-37...
  • Page 268 /µL] MXD# 1.0 [×10 /µL] [fL] NEUT# 8.1 [×10 /µL] 17) 7 The particle count equal to or less than LD is higher than the range. PLT is flagged with AG and other parameters are not flagged. 14-38 Sysmex XP-300...
  • Page 269 68.26% of Entire Distribution Area RDW-SD (RBC Distribution Width - SD) is set on 20% frequency level with the peak taken as 100%. The unit applied is fL (femto-liter = 10 • Definition of RDW-SD 100% RDW-SD Sysmex XP-300 14-39...
  • Page 270 This is the ratio of large platelets exceeding 12 fL discriminator and is calculated as the ratio of the particle count between the 12-fL fixed discriminator and Upper discriminator (UD) to the particle count between Lower discriminator (LD) and Upper discriminator (UD). 14-40 Sysmex XP-300...
  • Page 271 Frequencies of LD and UD are higher than the range. ×: • In the case of DW, when RDW-SD could not be analyzed. • In the case of MP, when RBC histogram has 2 or more peaks. Any of “ ,” “×,” and “High” is applicable. Sysmex XP-300 14-41...
  • Page 272 MCV, MCHC, and RDW-SD. • RBC Histogram Error - RU (2A) 3.57 [×10 /µL] 11.5 [g/dL] 41.8 [%] RU +117.1 [fL] 32.2 [pg] [fL] MCHC -27.5 [g/dL] RDW-SD RU +91.7 [fL] 185 [×10 /µL] 14-42 Sysmex XP-300...
  • Page 273 In this instance, RDW-CV is out of Patient Mark Limits. • RBC Histogram Error - MP (4) 4.80 [×10 /µL] 14.2 [g/dL] 41.5 [%] 86.5 [fL] 29.6 [pg] [fL] MCHC -34.2 [g/dL] RDW-CV MP +33.3 [fL] 216 [×10 /µL] Sysmex XP-300 14-43...
  • Page 274 Frequencies of LD and UD are higher than the range. ×: • In the case of DW, when PDW could not be analyzed. • In the case of MP, when PLT histogram has 2 or more peaks. Any of “ ,” “×,” and “High” is applicable. 14-44 Sysmex XP-300...
  • Page 275 PLT parameter is flagged with PU. Particle distribution width (PDW) is flagged with DW and its data are not output. MPV and P-LCR are flagged with PU and their data are not output. • Platelet Histogram Error - PU (2B) 171 [×10 /µL] ---.- [fL] ---.- [fL] P-LCR ---.- [%] [fL] Sysmex XP-300 14-45...
  • Page 276 4 2 or more peaks PLT is not flagged. Other parameters are flagged with MP and their data are not output. • Platelet Histogram Error - MP (4) 155 [×10 /µL] ---.- [fL] ---.- [fL] P-LCR ---.- [%] [fL] 14-46 Sysmex XP-300...

  • Page 277: Electric System

    The beam that has passed through the fluid is detected by the photo diode. And the signals is photoelectrically converted, A/D converted, and then sent to the HGB counting circuit for the calculation of the absorbance. Sysmex XP-300 14-47...

  • Page 278: Warranty

    Technical Information 14.7 Warranty All Sysmex instruments are warranted against defective material or workmanship for a period of 1 year, commencing on the installation date at the customer's premises. This warranty does not cover any defect, malfunction or damage due •...

  • Page 279: Appendix

    Technical Information 14.8 Appendix • Maintenance checklist • Reagent replenishing record XP-300 maintenance checklist Sysmex XP-300 14-49...
  • Page 280 Technical Information Reagent replenishing record 14-50 Sysmex XP-300...

  • Page 281: Gnu General Public License

    Person wishing to obtain the source code or detailed information concerning the software used with this product that is subject to the GNU General Public License should contact your local Sysmex representative. For the software used with this product that is not subject to the GNU General Public License, the source code may not be obtained, reverse engineered, decompiled, or disassembled.
  • Page 282 In addition, mere aggregation of another work not based on the Program with the Program (or with a work based on the Program) on a volume of a storage or distribution medium does not bring the other work under the scope of this License. 14-52 Sysmex XP-300...
  • Page 283 If any portion of this section is held invalid or unenforceable under any particular circumstance, the balance of the section is intended to apply and the section as a whole is intended to apply in other circumstances. Sysmex XP-300 14-53...
  • Page 284 INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. 14-54 Sysmex XP-300...
  • Page 285 If this is what you want to do, use the GNU Library General Public License instead of this License. Sysmex XP-300 14-55...
  • Page 286 Technical Information 14-56 Sysmex XP-300...

  • Page 287: Index

    Basic instrument settings .......... 5-15 Control material..........9-1, 14-2 Basic operation area ........... 6-2 Control method selection..........9-7 Before installation ............5-2 Control methods ............9-5 Blank Error ............. 13-12 Blood cell discrimination circuit ......14-29 Blood volume aspirated ..........14-2 Sysmex XP-300...
  • Page 288 Establishing reference values........10-3 Irregularity and roughness of printing ....14-16 Every 3 months (or every 4,500 samples) ....12-1 Every month (or every 1,500 samples) ..... 12-1 Examples of printed results on the graphic printer (GP) ............14-10 Sysmex XP-300...
  • Page 289 Peripheral devices (optional) ........5-13 Reagents list............12-38 Platelet Histogram Error Flag......... 14-44 Rear side ..............3-4 PLT discriminator........... 14-29 References ..............9-4 PLT Histogram ............14-40 Registering the operator ID ......7-12, 7-22 PLT Noise Error ............. 13-13 Sysmex XP-300...
  • Page 290 Starting up quality control chart screen ....9-24 Storage (transportation) condition ......14-1 Storage and shelf life after first opening..... 9-2 Stored data ..............8-11 Stored data screen ............. 8-12 Stored Error............. 13-15 STROMATOLYSER-WH.......... 4-2 Surface reflection ............ 14-16 Switching ON ............. 7-5 Sysmex XP-300...

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