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SYSMEX XE-5000 Quick Manual
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SYSMEX XE-5000 Quick Manual

Automated hematology system
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SYSMEX XE-5000
If you need further assistance, please contact
the Sysmex Technical Assistance Center:
Phone:
Fax:
Document Number: MKT-70-1096
TM
1-888-8SYSMEX
(1-888-879-7639)
1-847-996-4499
SYSMEX AMERICA, INC.
One Nelson C. White Pkwy.
Mundelein, IL 60060
Revision 1
Quick Guide
Page 1
Start-Up
QC Analysis
Sample
Processing
Sample Explorer/
Data Browser
Body Fluid Mode
HPC Mode
Reagent
Replacement
QC Setup
Maintenance

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Summary of Contents for SYSMEX XE-5000

  • Page 1 Data Browser Body Fluid Mode HPC Mode Reagent Replacement QC Setup Maintenance If you need further assistance, please contact the Sysmex Technical Assistance Center: Phone: 1-888-8SYSMEX (1-888-879-7639) Fax: 1-847-996-4499 SYSMEX AMERICA, INC. One Nelson C. White Pkwy. Mundelein, IL 60060...
  • Page 2 This Page Left Blank Intentionally Document Number: MKT-70-1096 Revision 1 Page 2...
  • Page 3 Select ‘RETRY’ on Background error screen on Main Unit to perform another Background check. Main Unit Log-on Screen To resolve a Background Error, refer to XE-5000 Information for Use, Troubleshooting Chapter. Reagent Volume Check Click [CONTROLLER] in the IPU Menu Tab.
  • Page 4 QC Analysis SYSMEX XE-5000 SAMPLER (AUTO) MODE ‘Control Entry Error’ occurs if lot is not entered in QC files. Warm controls to room temperature. Pre-mix controls by gentle end-to-end inversion until suspended. Place barcoded QC samples in rack, barcodes facing the front of the rack.
  • Page 5 QC Analysis SYSMEX XE-5000 To View Radar Charts Note: Radar Charts for Current Lot only. On IPU, click on QC icon. Click on Radar Tab. Choose level and mode. Control Data are displayed in green on Radar Chart. If any parameter is outside of lower limit (inner red ring) or outside of upper limit (outer red ring), then go to Control tab to view actual data on L-J Charts.

  • Page 6: Sample Processing

    Sample Processing SYSMEX XE-5000 SAMPLER (AUTO) MODE with BARCODES XE-5000 MANUAL OPEN MODE Verify XE Main Unit ‘Ready’ LED is on and ‘Manual’ is displayed on Verify XE green ‘Ready’ LED is on. Main Unit. Press [MANUAL] on XE Main Unit LCD panel.
  • Page 7 Capillary Screen Mode. Press [MANUAL] on XE Main Unit LCD. Press [↓] and select “1 Manual Mode”. Press [ENTER]. XE-5000 Manual Closed Mode Blood analyzed using Cap Piercer (without mixing or reading barcode). Verify ‘Ready’ LED is on. To change XE Main Unit to Closed Mode, press [MANUAL] on Main Unit LCD panel.
  • Page 8 Sample Explorer SYSMEX XE-5000 /Data Browser Finding A Sample Click [Sample Explorer]. Verify “Last 20” icon is de-selected (top blue line displays “Filter All”). Click sample number at top of list. From Tool Bar, click [Edit] and [Find]. Find dialog box is displayed. Enter Sample number or Patient ID.
  • Page 9 Go to the IPU Settings, HOST (HC) Setting. Click the empty box next to “Host(HC) Connect” to reconnect IPU and Host. Click [OK] to save the changes. The green HOST icon displays. The XE-5000 is now reconnected to the HOST. Record menu Document Number: MKT-70-1096...

  • Page 10: Analysis Parameters

    SYSMEX XE-5000 Summary of Body Fluid Analysis The body fluid analysis mode of the XE-5000 uses the 4 DIFF scattergram and the RBC distribution obtained from a specialized analysis sequence to calculate and display the WBC counts, mononuclear cell (MN) / polymorphonuclear cell (PMN) count and percentages, and the RBC (RBC-BF) counts found in the body fluid.
  • Page 11 Body Fluid Mode SYSMEX XE-5000 If the results of the background check is such that the blank value is at or below the acceptable limit, the READY LED lights and the Main Unit enters body fluid analysis ready status. 10. Mix the contents of the test tube gently but thoroughly.
  • Page 12 HPC Mode SYSMEX XE-5000 Summary of HPC Analysis The HPC (hematopoietic progenitor cell) analysis mode provides a rapid count of HPCs using peripheral blood and cord blood for screening purposes. The HPC count can help determine the optimal time for harvesting stem cells more quickly and at a lower cost.
  • Page 13 HPC Mode SYSMEX XE-5000 11. The results of HPC Mode are displayed on the Main Unit LCD screen. Samples analyzed in HPC Mode are marked with an “H” to the left of the Sample No. on the LCD screen. The results are also displayed on IPU screens. On the Sample Explorer screen samples analyzed in HPC analysis mode are marked with an “H”...

  • Page 14: Reagent Replacement

    Reagent Replacement SYSMEX XE-5000 When a reagent container is empty, an alarm sounds and error message displays. Press [HELP] on XE-Main Unit. Select [OK]. Reagent Setting Screen is displayed with “Replace” next to reagent that needs replacing. Verify which reagent to change by matching reagent abbreviation to proper name of reagent.
  • Page 15 QC Setup SYSMEX XE-5000 Input New Lot Number Click Control tab. Select Level, Mode, and New lot. Click Lot No. A box displays a window for lot information. Input 8 digit lot number of new control. Click or tab to move cursor to expiration date.
  • Page 16 SYSMEX XE-5000 Save QAP Data Using Sysmex Insight • Sysmex Insight icon MUST be used for saving QC data for QAP. • Review data and if desired, edit (delete) control data prior to submission. Ensure all QC data is plotted and no analysis error data is included (- - -) or (+ + +).

  • Page 17: Maintenance

    Maintenance SYSMEX XE-5000 XE Pneumatic Unit: Inspect Trap Chamber for Fluid • Visually inspect Trap Chamber for fluid. • If fluid is present, turn Pneumatic Unit off. Remove Trap Chamber by turning clockwise. Discard fluid. Dry chamber and re-assemble trap on Pneumatic Unit.
  • Page 18 When beeping stops and ‘READY’ LED turns off, remove tube of bleach. After about 15 minutes, Main Unit displays “Please POWER Off.” To continue analysis, select “RESTART” on LCD screen. After auto-rinse and background check is completed, XE-5000 is “Ready”. Weekly: Power IPU off after performing Daily Shutdown...