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SYSMEX KX-21 Operator's Manual
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SYSMEX KX-21 Operator's Manual

Automated hematology analyzer
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CHAPTER 1:
CHAPTER 2:
CHAPTER 3:
CHAPTER 4:
CHAPTER 5:
CHAPTER 6:
CHAPTER 7:
CHAPTER 8:
CHAPTER 9:
CHAPTER 10: INSTRUMENT SETUP
APPENDIX A: INSTALLATION
APPENDIX B: TECHNICAL INFORMATION
INDEX
Copyright © 1997 - 2000 by SYSMEX CORPORATION
All rights reserved. No part of this Operator's Manual may be
reproduced in any form or by any means whatsoever without
prior written permission of SYSMEX CORPORATION.
INTRODUCTION
SAMPLE ANALYSIS
DISPLAY AND PROCESSING OF ANALYSIS RESULTS
MAINTENANCE AND SUPPLIES REPLACEMENT
QUALITY CONTROL
CALIBRATION
TROUBLESHOOTING
ADJUSTMENT
FUNCTIONAL DESCRIPTION
SYSMEX CORPORATION
OPERATOR'S MANUAL
AUTOMATED HEMATOLOGY ANALYZER
KX-21
KOBE, JAPAN
Code No. 461-2261-1
PRINTED IN JAPAN
Date of Last Revision: January 2000

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Do you have a question about the KX-21 and is the answer not in the manual?

Questions and answers

Zameer
March 3, 2025

How to solved pressure errors

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1 comments:
Mr. Anderson
March 3, 2025

To solve pressure errors on the SYSMEX KX-21, follow these steps:

1. Check the Pressure Value – Verify if the pressure is at the correct level (0.5 kg/cm²).
2. Adjust the Pressure – If incorrect, adjust the pressure using the control knobs.
3. Inspect for Leaks – Ensure there are no air leaks in the pneumatic system.
4. Clean the SRV and Transducer – Perform cleaning as needed to maintain proper function.
5. Check Fixing Screws – Ensure all screws related to the pneumatic unit are properly tightened.
6. Reset the Instrument – Restart the device to check if the error persists.

If the issue continues, further troubleshooting or service may be needed.

This answer is automatically generated

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Summary of Contents for SYSMEX KX-21

  • Page 1 APPENDIX B: TECHNICAL INFORMATION INDEX SYSMEX CORPORATION KOBE, JAPAN Copyright © 1997 - 2000 by SYSMEX CORPORATION All rights reserved. No part of this Operator’s Manual may be Code No. 461-2261-1 reproduced in any form or by any means whatsoever without PRINTED IN JAPAN prior written permission of SYSMEX CORPORATION.
  • Page 2 Manual. • Patient names and doctor names are entered for information and illustration purposes only, and do not imply real specific persons. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 3 RECEIVING INSTRUCTIONS The KX-21 has been thoroughly tested before shipment, and has been packaged carefully to prevent damage from shipping and handling. Reagents and options have also been sent and will arrive at approximately the same time as the analyzer. Follow these guidelines when the system arrives: •...
  • Page 4 Caution on Diagnosis • This product is a clinical instrument for screening of abnormalities. CAUTION Clinical judgments by physicians should take into account results from clinical examinations and other test results besides hematology result. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 5 Those might cause short circuit or smoke emission. If such trouble should occur, turn off the power supply immediately and pull off the power plug from the wall socket. Then contact Sysmex service representative for inspection. • Do not touch the electrical circuits inside the cover. Especially if your hands are wet, there is a hazard that electrical shock may result.
  • Page 6 Otherwise, the wire may break causing fire or electrical shock. • When connecting the instrument to a peripheral (host computer), be sure to switch off the power supply beforehand. Otherwise, electrical shock or instrument failure may result. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 7 • If the instrument has developed a trouble by any chance, a person in charge of it should take steps within the range specified in the OPERATOR’S MANUAL. As to troubles other than mentioned in it, contact Sysmex service representative for repair. • Unpacking, installation, and confirmation of initial operation must be done by Sysmex service representative.
  • Page 8 Describes the installation method of the KX-21. Appendix A: Installation Technical reference data describing the specification for Appendix B: Technical Information host output format. (The host output function is an option.) Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 9 Carefully read the OPERATOR'S MANUAL for correct use of the unit. Keep this MANUAL handy after reading. It will continue to be of your service in finding specific information about this instrument. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 10 • LCD and printing described in this manual may differ from that in practice. • Due to the improvement of the product, the content of this manual may not conform with the product. Sysmex KX-21 Operator’s Manual -- Revised October 1998 VIII...
  • Page 11 Display of Analysis Result ..........2-24 Printing of Analysis Result..........2-25 LIMITATIONS ..............2-26 Cell Count Parameters ............2-26 Limitation of Hemoglobin..........2-27 EXPECTED RESULTS ............2-28 STOPPING THE PNEUMATIC UNIT ........2-29 EXECUTION OF SHUTDOWN..........2-30 Shutdown Procedure ............2-30 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 12 Clean WBC/RBC Transducer Aperture ........4-28 Replace Waste Tank............4-32 SUPPLIES REPLACEMENT ..........4-33 Replenish Reagent............4-33 Replace Fuse ...............4-36 Replace Printer Paper .............4-37 Supplies List..............4-40 CONFIRMATION OF CYCLE NUMBER........4-41 Operation Procedure ............. 4-41 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 13 Reference Values ............6-1 Calibration Flow Chart............. 6-2 AUTOMATIC CALIBRATION ..........6-3 Executing Automatic Calibration Program ......6-3 Automatic Calibration Procedure......... 6-4 MANUAL CALIBRATION ............. 6-9 Calculating Calibration Value ..........6-9 Manual Calibration Procedure ...........6-10 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 14 ADJUSTMENT OF PRESSURE AND VACUUM ......8-2 Location of Control Knobs ..........8-2 Pressure and Vacuum Display ........... 8-3 Adjusting Pressure to 0.5 kg/cm ........8-5 Adjusting Vacuum to 250 mmHg ........8-6 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 15 DATE/TIME SETTINGS ............10-7 PATIENT LIMIT............... 10-10 QC SETTINGS ..............10-13 HOST SETTINGS (OPTION) ..........10-16 PRINTER SETTINGS ............10-21 PRINT SET VALUES ............10-24 PERIPHERAL SETTINGS ........... 10-25 FACTORY SETTINGS ............10-27 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 16 Connect Waste Line............A-9 SET PRINTER PAPER............A-10 CONNECT POWER CORD ........... A-12 TURN POWER ON ............. A-12 APPENDIX B: TECHNICAL INFORMATION OUTPUT FORMAT FOR HOST COMPUTER (Option) ....B-1 Hardware..............B-1 Software..............B-3 INDEX Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 17 EMERGENCY STOP PROCEDURE..........1-11 ALARM SOUNDS ...............1-11 CONTENTS OF PACKAGE............1-11 INSTALLATION ENVIRONMENT...........1-12 12.1 Installation and Relocation ..........1-12 12.2 Grounding ..............1-12 12.3 Installation Space............1-13 12.4 Installation Environment ..........1-14 INSTRUMENT SPECIFICATIONS...........1-15 MENU TREE..............1-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 18 WBC, RBC, and platelets, along with data of 18 parameters, as the analysis results. Chapter 1 introduces the overview of the instrument, analysis procedure, etc. that we recommend you to read before using the KX-21. The main contents of Chapter 1 are as follows: Overview of Instrument The important functions of the KX-21 and the options for efficient operation are explained.
  • Page 19 INTRODUCTION OVERVIEW OF INSTRUMENT The KX-21 performs speedy and accurate analysis of 18 parameters in blood and detects the abnormal samples. To assure easy sorting of abnormal samples in the laboratory, the instrument displays abnormal analysis data with abnormal marks attached on the LCD screen.
  • Page 20 • Serial interface: Outputs analysis data and QC data to the host computer. Ordering Information: OUTPUT UNIT NO. 1 KX-21 (Part No. 973-4531-1) OUTLINE OF OPERATION Two analysis modes are available with the KX-21: whole blood mode and pre-diluted mode. Analysis procedures in these modes are listed below. Whole blood mode Pre-diluted mode Check before turning ON the power.
  • Page 21 Mean RBC volume (fL) in whole blood, which is calculated by Hct/RBC. MCH (Mean RBC hemoglobin) Mean hemoglobin volume (pg) per RBC, which is calculated by Hgb/RBC. MCHC (Mean RBC hemoglobin concentration) Mean hemoglobin concentration (g/dL), which is calculated by Hgb/Hct. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 22 • When analyzing in the pre-diluted mode, only the CBC8 parameters are output. • The RBC distribution width is the switchover type between RDW-SD and RDW-CV. * For Investigational Use Only in the United States of America. Not a reportable parameter. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 23 INTRODUCTION PANEL KEYBOARD The KX-21 is provided with the 22-key panel keyboard. SAMPLE No. ENTER SELECT MODE HELP SHUTDOWN Figure 1-7-1: Panel Keyboard Name Function SAMPLE No. Used to set a sample No. and QC File No. ENTER Used to fix a sample No., selected menu, etc.
  • Page 24 Status Display Status of the unit is displayed. Menu Display Area Useable menus for each screen are displayed. Analysis Result The area for displaying analysis result Display Area Table 1-8-1: Contents of Display Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 25 INTRODUCTION 8 . 2 Status Display Messages The KX-21 displays instrument status message on the LCD screen. This section gives the meanings of status messages displayed on the LCD screen. Display Message Meaning The instrument is making self-check. When the power is turned ON, the instrument checks itself automatically.
  • Page 26 No. 1" in the left upper corner shows QC file Judgement MCHC The instrument is ready for L-J control. "FILE FILE No.1 Ready No. 1" in the left upper corner shows QC file Data Judgement MCHC Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 27 When data is automatically output to the host computer, " * " mark lights up in the right Ready upper corner of the screen. This applies only when an optional serial interface is incorporated. 1-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 28 1 0 . ALARM SOUNDS The KX-21 indicates different situations by five kinds of alarm sounds: Key sound (single beep) Sounds about 0.1 second when a key on the panel keyboard is pressed.
  • Page 29 1 2 . 1 Installation and Relocation The KX-21 is installed by Sysmex Service representative. In case relocation becomes necessary after installation, contact your Sysmex service representative. Pay careful attention to this because if relocation, etc. of the instrument should be conducted by the customer, resulting in any trouble, warranty would not be applied even in the warranty period.
  • Page 30 • When the diluent (CELLPACK) tube is more than 2 m long, it may be impossible to have reagent aspirated. Avoid setting CELLPACK at a level higher than the instrument; otherwise, reagent may flow into the vacuum line, possibly damaging the instrument. 1-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 31 • Avoid a place that can be exposed to direct sunlight. • Choose a well-ventilated place. • Avoid a place close to a wireless telegraph or communication facility where high frequency waves can be generated or radio interference can occur. 1-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 32 Diluent (CELLPACK): Approx. 30 mL WBC/HGB lyse reagent (STROMATOLYSER-WH): Approx. 1.0 mL Throughput Approx. 60 samples/hour Analysis principle WBC: DC detection method RBC: DC detection method HGB: Non-cyanide hemoglobin analysis method 1-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 33 /µL or less Pre-diluted mode ±5% or ±0.3 × 10 WBC: /µL or less ±3% or ±0.05 × 10 RBC: /µL or less ±8% or ±15 × 10 PLT: /µL or less 1-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 34 According to the type of protection against electric shock: Class I Equipment According to the degree of protection against electric shock: Type B Equipment 1-17 Sysmex KX-21 Operator’s Manual -- Revised January 2000 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 35 SAMPLE No. Chapter 2, 4 Chapter 2, 4 MODE HELP Chapter 7, 1 Chapter 2, 6 SHUTDOWN : Applicable when the option is provided. 1-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 36: Table Of Contents

    DISPLAY AND PRINTING OF ANALYSIS RESULT ....2-24 Display of Analysis Result..........2-24 Printing of Analysis Result ..........2-25 LIMITATIONS..............2-26 Cell Count Parameters.............2-26 Limitation of Hemoglobin..........2-27 EXPECTED RESULTS............2-28 STOPPING THE PNEUMATIC UNIT ........2-29 EXECUTION OF SHUTDOWN ..........2-30 Shutdown Procedure ............2-30 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 37: Chapter 2 Sample Analysis

    • In the pre-diluted mode, particle distribution curve and particle distribution analysis data are not output, and the output is confined to only the CBC8 parameter. Sysmex KX-21 Operator’s Manual -- Revised October Sysmex KX-21 Operator’s Manual -- Revised December 1999...

  • Page 38: Analysis Procedure Flow Chart

    Whole Blood Mode Pre-Diluted Mode Analysis Analysis Page 2-9 Page 2-16 Display and Printing of Page 2-24 Analysis Result Page 2-26 Execution of Shutdown Turning OFF the Power Table 2-1-1: Analysis Procedure Flow Chart Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 39: Start-Up Procedure

    • After unpacking, take care to prevent entry of dust, dirt, bacteria, etc. which could impair proper analysis. CELLPACK 20 L 60 days 90 days STROMATOLYSER-WH 500 mL Table 2-2-1: Expiration After Opening the Seal Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 40 Figure 2-2-1: Inspection of Printer Paper CAUTION: • Use the printer paper specified by Sysmex. Avoid using print paper that has its end fixed to the core. Such paper can cause failure. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 41: Turning On The Power And Self-Check

    Figure 2-2-2: Initial Display Next, the instrument makes self-check of motor operation, scheduled maintenance items, etc. The LCD screen displays "Please wait." during this period. Please wait. Figure 2-2-3: LCD Screen during Self-check Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 42 • When self-check reveals any error, an error message will appear on the LCD screen. In this case, turn OFF the power once, then turn it ON again. If the error still occurs, contact Sysmex service representative. • To ensure optimum operation of the instrument, service counters are provided for the components which require scheduled maintenance.
  • Page 43 MCHC ---.-g/dL ---.-fL × /µL PDW ---.-fL ---.-fL P-LCR ---.-% 1:M.Discri. 2:Print 3:Paper Feed Figure 2-2-5: Ready Status NOTE: • When the power is on, the next sample No. is displayed "1." Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 44: Quality Control

    • Analysis mode to analyze a control blood Control blood is analyzed in the QC analysis mode. For analysis procedure and examination of analysis result, refer to Chapter 5: QUALITY CONTROL. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 45: Procedures In Each Analysis Mode

    Tubes up to 80 mm in height should be used. The volume of sample that can be aspirated is as follows: Approx. 50 µL Volume of sample aspirated Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 46 • The analysis mode selected here is maintained after completion of analysis, until it is switched to the other analysis mode. • At the time of turning-on the power, the system is in the whole blood mode. 2-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 47 4) Sample No. is not incremented. 5) Judgment on abnormal histogram is not made. 6) When one attempts to make analysis with the sample No. of "0" (zero), the buzzer sounds during sample aspiration. 2-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 48 When you attempt to do so, an alarm sounds. • In the status of waiting for sample No. input ("Not Ready"), press [SAMPLE No.] or [SELECT] key. The screen returns to the Analysis screen without updating sample No. 2-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 49 Remove the plug while taking care not to allow blood scatter. Figure 2-4-5: Removing the Plug Set the tube to the sample probe, and in that condition, press the start switch. Start Switch Figure 2-4-6: Pressing the Start Switch 2-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 50 • To remove the tube, lower it straight down. Take care not to bend the sample probe. NOTE: • The sample probe is automatically rinsed, so there is no need to wipe it clean. 2-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 51 (1) to (4). NOTE: • Sample No., if not changed, is automatically incremented by 1, although "0" (zero) is not incremented. → [Example] 123 999999 → → 12-3 12-4 → 12-999 12-000 2-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 52: Pre-Diluted (Pd) Mode

    Tubes up to 80 mm in height should be used. The volume of sample is as follows: Approx. 20 µL or more Volume of whole blood required Approx. 200 µL Volume of sample aspirated 2-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 53 When preparing a 1:26 dilution sample, use the tools listed below: • Diluent (CELLPACK) • Micro-tube (MT-40, etc.) • Capillary tube • A 500 µL transfer pipette • A container, such as erlenmeyer flask or beaker • A syringe 2-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 54 • The analysis mode selected here is maintained after completion of analysis, until it is switched to the other analysis mode. • At the time of turning-on the power, the system is in the whole blood mode. 2-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 55 4) Sample No. is not incremented. 5) Judgment on abnormal histogram is not made. 6) When one attempts to make analysis with the sample No. of "0" (zero), the buzzer sounds during sample aspiration. 2-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 56 When you attempt to do so, an alarm sounds. • In the status of waiting for sample No. input ("Not Ready"), press [SAMPLE No.] or [SELECT] key. The screen returns to the Analysis screen without updating sample No. 2-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 57 Remove the plug while taking care not to allow blood scatter. Figure 2-4-12: Removing the Plug Set the micro-tube to the sample probe, and in that condition, press the start switch. Start Switch Figure 2-4-13: Pressing the Start Switch 2-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 58 • To remove the micro-tube, lower it straight down. Take care not to bend the sample probe. NOTE: • The sample probe is automatically rinsed, so there is no need to wipe it clean. 2-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 59 (1) to (4). NOTE: • Sample No., if not changed, is automatically incremented by 1, although "0" (zero) is not incremented. → [Example] 123 999999 → → 12-3 12-4 → 12-999 12-000 2-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 60: Display And Printing Of Analysis Result

    37.0pg 37.0pg MCHC + 44.8g/dL MCHC + 44.8g/dL × × /µL /µL 1:M.Discri. 2:Print 3:Paper Feed 1:M.Discri. 2:Print 3:Paper Feed (Whole Blood Mode) (Pre-diluted Mode) Figure 2-5-1: Analysis Result Display Screen 2-24 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 61: Printing Of Analysis Result

    /µL × MXD# /µL × NEUT# /µL [fL] 98.3 fL [fL] 8.3 fL 9.1 fL P-LCR 14.8 % Figure 2-5-2: Printing of Analysis Result 2-25 Sysmex KX-21 Operator’s Manual -- Revised January 2000 Sysmex KX-21 Operator’s Manual -- Revised October...

  • Page 62: Limitations

    Instrument count is affected by either an increase or decrease in the result which is sample dependent. CAUTION: • WBC results may be elevated erroneously due to unlysed red cells in patients with hemoglobinopathies, severe liver disease or in neonates. 2-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 63: Limitation Of Hemoglobin

    Performance specifications for hemoglobin results are assured only when instrument environment and sample requirements are observed. The hemoglobin method available for this analyzer cannot detect sulfhemoglobin, verdohemoglobin choleglobin or other unusual degradation products of hemoglobin. 2-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 64: Expected Results

    SAMPLE ANALYSIS EXPECTED RESULTS Reference intervals (normal population reference ranges) were developed for the KX-21 using normal individuals. The ranges for each parameter --- WBC, RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW-CV, RDW-SD, Platelet, PDW*, MPV, P-LCR*, Lym% and #, Mxd% and #, and Neut% and # were determined and displayed in Table 1-16-1.

  • Page 65: Stopping The Pneumatic Unit

    SAMPLE ANALYSIS STOPPING THE PNEUMATIC UNIT The KX-21, when left non-operating for 15 minutes, automatically stops the pneumatic unit. It is also possible to manually stop the pneumatic unit using the following procedures: Press [SELECT] key in the Ready status. The Select Menu screen appears.

  • Page 66: Execution Of Shutdown

    * Shutdown * Ready Aspirate CELLCLEAN. It will take approx. 5 minutes. 3:Cancel Figure 2-9-1: Shutdown Screen NOTE: • When [3] key is pressed, shutdown is canceled and the Analysis screen returns. 2-30 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 67 After that, shutdown is executed automatically. * Shutdown * Rinsing Please wait. Figure 2-9-3: Shutdown Execution Screen CAUTION: • To remove CELLCLEAN, lower it straight down. Take care not to bend the sample probe. 2-31 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 68 * Shutdown * Shut-down sequence was completed. Turn OFF the power. Figure 2-9-4: Shutdown Completion Message Screen NOTE: • Shutdown will take approximately 5 minutes. 2-32 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 69 PROCESSING STORED DATA ..........3-11 3.1 Executing and Quitting Stored Data Processing Program .... 3-11 Stored Data Screen............3-12 Deletion..............3-15 Printing..............3-16 HC Output (Option)............3-16 3.6 Correcting a Sample Number..........3-17 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 70: Introduction

    The analysis data stored in the instrument can be read at anytime. Description is made on the display contents of stored data, what they mean, deletion by marking, and output to external devices. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 71: Processing Latest Samples

    Use [ ] key or [ ] key to change over pages. P1. All Analysis Data P2. Particle Distribution P3. CBC8 Parameter Display Screen Display Screen Display Screen Figure 3-2-1: Page Selection in the Analysis Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 72 Date/Time Analyzed No.123458 WB 31/12 23:59 Mode Indication × /µL × 3.97 /µL 14.7g/dL 32.8% - 82.6fL 37.0pg MCHC + 44.8g/dL × /µL 1:M.Discri. 2:Print 3:Paper Feed Figure 3-2-4: CBC8 Parameter Display Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 73 • The marks are added with the priority order of 1, 2, and 3. • The Mark Limits with "+" and "-" can be set by the customer. For details, refer to Chapter 10, Section 4: Patient Limit . Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 74 The WBC Particle distribution chart is displayed. RBC Particle Distribution Chart The RBC Particle distribution chart is displayed. PLT Particle Distribution Chart The PLT Particle distribution chart is displayed. Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...

  • Page 75: Manual Discrimination

    Using [ ] key or [ ] key, select a desired page of the M. Discri. screen. The M. Discri. screen consists of three pages. P1. M. Discri. Screen (WBC) P2. M. Discri. Screen (RBC) P3. M. Discri. Screen (PLT) Figure 3-2-6: Page Selection in the M. Discri. Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 76 Press [ENTER] key. The discriminator for manual discrimination is set and the discriminator selected on the Particle Distribution Chart is shown with solid line. Sysmex KX-21 Operator’s Manual -- Revised December 1999 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 77 When [SELECT] key is pressed without pressing [ENTER] key, the Discri. position is not changed, and the status returns to the Discri. position selection status. When you want to change another Discri., repeat the above (2) - (6) operation. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 78 No.123458 WB 31/12 23:59 LYM% 31.2% MXD% 19.6% NEUT% 49.2% × LYM# /µL × MXD# /µL × NEUT# /µL - 25.6fL 7.9fL 9.3fL P-LCR 9.5% 1:M.Discri. 2:Print 3:Paper Feed Figure 3-2-10: Analysis Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 79: Printing

    2 . 4 Printer Paper Feed Press [3] key on the Analysis screen and select "3: Paper Feed." While the key is being held in, paper feed continues. 3-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 80: Processing Stored Data

    Press [SELECT] key on the Stored Data screen. The Analysis screen returns. NOTE: • The sample No. and analysis mode on the Analysis screen return to the status before the execution of the stored data processing program. 3-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 81: Stored Data Screen

    [ ], [ ] key: Changes over the page. [ENTER] key: Attaches or deletes marks. [SAMPLE No.] key: Allows you to correct the sample number at the underline cursor. 3-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 82 Nos. QC data are displayed in the format of "QC + File No." Mode Indication The analysis mode for stored data is displayed. "PD" is indicated for the pre-diluted mode while no indication is given for the whole blood mode. 3-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 83 Date Analyzed The date when the analysis data in the underline cursor position was analyzed is displayed. Manual Discrimination Data As to data changed through manual discrimination, analysis values are reverse- displayed. 3-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 84: Deletion

    Press [ENTER] key to execute the selected process. When you press [SELECT] key, the process is canceled. NOTE: • Before deleting data, make sure the KX-21 completes any output operation to the printer or host computer. 3-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 85: Printing

    * Stored Data * Figure 3-3-7: Screen during Output to Host NOTE: • Output to the host computer cannot be interrupted. 3-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 86: Correcting A Sample Number

    No.] key. The selected sample number will be highlighted. Use the numeric keypad to enter a new sample number, then press the [ENTER] key. When the confirmation message appears, select the [Set] button. 3-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 87 7.4 Replace Waste Tank ............4-32 SUPPLIES REPLACEMENT..........4-33 8.1 Replenish Reagent ............4-33 8.2 Replace Fuse..............4-36 8.3 Replace Printer Paper............4-37 8.4 Supplies List..............4-40 CONFIRMATION OF CYCLE NUMBER........4-41 9.1 Operation Procedure ............4-41 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 88: Introduction

    Replace the waste tank. This chapter describes supplies replacement together with these maintenance items. • Supplies Replacement Replenish reagent. Replace fuses. Replace printer paper. Sysmex KX-21 Operator’s Manual -- Revised January 2000 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 89: Maintenance Checklist

    MAINTENANCE AND SUPPLIES REPLACEMENT KX-21 MAINTENANCE CHECKLIST Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 90 Da t e Exp i ry Signature E x p i r y L o t N o . D a t e Signature Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 91: Daily Maintenance And Procedure

    In executing shutdown, set CELLCLEAN to the sample probe and press the start switch in that status. While "Aspirating" is being displayed on the screen, keep holding CELLCLEAN in the same state. Start Switch Figure 4-3-2: Pressing the Start Switch Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 92 * Shutdown * Shut-down sequence was completed. Turn OFF the power. Figure 4-3-4: Shutdown Completion Message Screen NOTE: • Shutdown will take approximately 5 minutes. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 93: Check Trap Chamber Level And Discard

    • If liquid collects everyday, the hydraulic system may have failed. CAUTION Contact Sysmex service representative. • Pay attention to the direction of the float in the chamber. Place it with its pointed end facing upward. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 94: Weekly Maintenance And Procedure

    CAUTION If analysis is made with the screw loosened, air can enter the system and affect the data. Wash the SRV tray using tap water. Tray Figure 4-4-2: Washing the SRV Tray Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 95 If analysis is made with the screw loosened, air can enter the system and affect the data. CAUTION: • Mount the SRV tray properly as to the face/bottom and the direction. Close the front cover of the main unit. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 96: Monthly Maintenance And Procedure

    • Even when the above message is not displayed, you can execute the waste chamber cleaning by choosing "7: Maintenance" on the Select Menu screen, then "1: Clean W. Chamber" on the Maintenance Menu screen. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 97 If the skin or clothes should come in touch with it, flush it away using plenty of water. Otherwise, it can damage the skin or clothes. 4-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 98 • When this operation is completed, the waste chamber cycle counter is automatically reset. For the confirmation of waste chamber cycle number, refer to Chapter 4, Section 9: CONFIRMATION OF CYCLE NUMBER . 4-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 99: Clean Transducer (Rinse Sequence)

    "2: Clean Transducer" on the Maintenance Menu screen. • If this rinse sequence fails to remove clogging, clean with the brush. Regarding cleaning with the brush, refer to Chapter 4, Section 7.3: Clean WBC/RBC Transducer Aperture. 4-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 100 Until the transducer is cleaned, the above message is displayed at start-up. Open the front cover of the main unit. Open the transducer cover. Figure 4-5-7: Opening the Transducer Cover 4-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 101 It may overflow, possibly causing electric shocks or leakage. CAUTION: • Take care not to have detergent (CELLCLEAN) adhere to the chamber side wall. Close the transducer cover. Close the front cover of the main unit. 4-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 102 • When this operation is completed, the transducer cycle counter is automatically reset. For the confirmation of transducer cycle number, refer to Chapter 4, Section 9: CONFIRMATION OF CYCLE NUMBER . 4-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 103: Every 3-Month Maintenance And Procedure

    "3: Cancel" on the Scheduled Maint. screen and executed cleaning when the power was off, reset the cycle counter, making reference to Chapter 4, Section 6.2: Reset SRV Cycle Counter . 4-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 104 Turn off the power of the main unit and wait approximately 30 seconds. Open the front cover of the main unit. Remove the sample rotor valve (SRV) tray. Probe Fixing Screw Tray Figure 4-6-3: Removing the Tray 4-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 105 (SRV) is removed. Remove the SRV fixing screw. SRV Fixing Screw Loosen Figure 4-6-5: Removing the SRV Fixing Screw 4-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 106 If left as it is, it can cause current leakage or electric shocks. NOTE: • The valve components are in close contact with one another. They can be easily removed when you gently twist and slide each one. 4-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 107 (12) Install one each valve at a time, and assemble the sample rotor valve (SRV) in the reverse order to disassembly. 4-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 108 NOTE: • When this operation is completed, the SRV cycle counter is automatically reset. For confirmation of SRV cycle number, refer to Chapter 4, Section 9: CONFIRMATION OF CYCLE NUMBER . 4-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 109: Reset Srv Cycle Counter

    Press [ENTER] key. The Rest SRV Counter screen appears. *Reset SRV Counter* SRV cycle counter will be reset. Cycle No. after cleaning. 7567 Date last serviced 31/12/97 1:Reset 3:Cancel Figure 4-6-11: Reset SRV Counter Screen 4-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 110 • Press [3] key to select "3: Cancel." The cycle counter is not reset, and the Analysis screen returns. • For confirmation of SRV cycle counter, refer to Chapter 4, Section 9: CONFIRMATION OF CYCLE NUMBER . 4-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 111: As-Needed Maintenance And Procedure

    • On the Menu screen, each menu can be selected directly by entering a numeral from the numeric keys. * Auto Rinse * Please wait. Figure 4-7-2: Auto Rinse Execution Screen 4-24 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 112 0.3 [×10 /µL] or less 0.02 [×10 /µL] or less 0.1 [g/dL] or less 10 [×10 /µL] or less Table 4-7-1: Background tolerance 4-25 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1999...

  • Page 113: Clean Rinse Cup

    Rinse Cup Figure 4-7-3: Pushing Down the Rinse Cup Remove the rinse cup in the order of 1, 2, and 3 as shown below. Rinse Cup Figure 4-7-4: Removing the Rinse Cup 4-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 114 • Make sure the rinse cup is pushed all the way up with the sample probe inserted in the hole. If the power is turned on with the rinse cup staying lowered, "Rinse Motor Error" will occur, making it impossible to continue the operation. 4-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 115: Clean Wbc/Rbc Transducer Aperture

    Press [ENTER] key. Reagent in the transducer chamber is automatically drained. NOTE: • On the Menu screen, each menu can be selected directly by entering a numeral from the numeric keys. 4-28 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 116 Loosen the screw and open the transducer cover. Loosen Screw Transducer Cover Figure 4-7-9: Opening the Transducer Cover • When the power is on, never open the transducer cover. This is to WARNING prevent possible electric shocks. 4-29 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 117 Apply CELLCLEAN on the brush provided, and dab by lightly prodding the brush against the transducer aperture. Aperture Brush Figure 4-7-12: Cleaning the Aperture 4-30 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 118 CAUTION current leakage or electric shocks can occur. Close the transducer cover and main unit front cover. Then turn on the power. Check to see that background error has not occurred. 4-31 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 119: Replace Waste Tank

    Tube Tape Waste Figure 4-7-13: Waste Container • When using a used reagent container as the waste container, make CAUTION sure to clearly mark it that it is the waste container. 4-32 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 120: Supplies Replacement

    • Press [3] key on the HELP screen and select "3: Cancel" to return to the Analysis screen. Although Analysis screen displays the Not Ready status, the processing, etc. of stored data can be performed. 4-33 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 121 • When replacing lyse, you do not need to disconnect the float switch. If disconnected, connect it securely in prior to starting analysis. Starting analysis with disconnected float switch will give you an error message "No Lyse". 4-34 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 122 Enter the contents of replenishment in Reagent Replenishment Record. NOTE: • If you replenish lyse before error message "No Reagent" appears, execute "4: Replace Lyse" on the Select Menu screen. 4-35 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 123: Replace Fuse

    Fuse 250 V 2 A No. 19195 Time Lag Table 4-8-2: Fuse Rating • For continued protection against risk of fire, use the fuse of the specified CAUTION type and rating. 4-36 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 124: Replace Printer Paper

    Open the front cover of the main unit. Turn the release lever up to free the paper. Release Lever Figure 4-8-4: Releasing the Lever 4-37 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 125 • When any printer paper remains, remove it together with the paper core. Pass the printer paper as shown below, and turn the release lever down to secure. Figure 4-8-6: Securing the Release Lever 4-38 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 126 Cut off any printer paper extending from the upper part of the printer. Figure 4-8-7: Cutting Off Paper Close the front cover of the main unit. Press [1] key and output any print data that is remaining on the memory. 4-39 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 127: Supplies List

    Chapter 4, Section 8.2: Replace Fuse ST4-3.15A-N1 (117 VAC Spec.) 921-0351-8 Paper Thermal F1-2 Chapter 4, Section 8.3: Replace Printer Heat-Sensitive Paper Paper (5 rolls per pack) Table 4-8-4: Consumable List 4-40 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 128: Confirmation Of Cycle Number

    Using [ ] key or [ ] key, move the cursor to select "5: Status Display." Press [ENTER] key. The Status Display screen 1 appears. NOTE: • On the Menu screen, each menu can be selected directly by entering a numeral from the numeric keys. 4-41 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 129 * Scheduled Maint. * Clean the Waste Chamber. It will take approx. 15 minutes. Cycle No. after cleaning. 2567 Date last serviced 31/12/97 1:Exec.Clean 3:Cancel Figure 4-9-3: Scheduled Maintenance Message Screen 4-42 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 130 Select QC File ............... 5-5 Erase All..............5-6 Set TARGET/LIMIT Values..........5-7 Execute Control ............5-9 2.7 Execute L-J Control............5-18 2.8 Exit from QC Control Program...........5-24 DELETION ................5-25 PRINT................5-27 HC OUTPUT (OPTION) ............5-29 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 131: Chapter 5 Quality Control

    EIGHTCHECK-3WP is the quality control material recommended by Sysmex to monitor the performance of the KX-21 analyzer. This product is supplied with three control levels, at least two levels should be run every 8 hours of operation or in accordance to regulations applicable to your laboratory.

  • Page 132: Qc Chart Screen

    QC Chart screen [1/3]: WBC, RBC, HGB QC Chart screen [2/3]: HCT, MCV, MCH QC Chart screen [3/3]: MCHC, PLT The number of control data plotted currently. In the case of control, the average represents a data. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 133 The vertical line cursor can be moved to the right or left using [ ] key or [ ] key. The QC data of the plot shown by the line cursor, along with analysis date, are displayed. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 134: Qc Analysis Procedure

    Execute X Control Page 5-9 Page 5-18 Delete QC Data Print HC Output Page 5-27 Page 5-25 Page 5-29 Exit from QC Program Page 5-24 Figure 5-2-1: QC Analysis Procedure Flow Chart Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 135: Execute Qc Program

    • Pressing [C] key erases the character and causes the cursor to move to the left. • Numerics of 1 - 6 can be entered as a FILE No. Any other numerics cannot be entered. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 136: Erase All

    For how to select the QC File, refer to Section 2.3: Select QC File in this chapter. Press [3] key to select "3. Erase All." The Erase All Confirmation message appears. OK to erase all? Figure 5-2-5: Erase All Confirmation Message Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 137 × /µL Press [SELECT] to exit. Figure 5-2-7: QC File Setting Screen Using [ ] key or [ ] key, move the cursor to select control parameters. Eight parameters can be set. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 138 Cancels the changed settings and returns to the QC Chart screen. Press [ENTER] key to execute the selected process. NOTE: • QC chart will be drawn only if the TARGET and LIMIT values are both entered appropriately. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 139: Execute X Control

    • In analyzing control blood, always wear rubber gloves. After WARNING completion of work, wash the hands with disinfectant. If your hands are contaminated by blood, etc., infection of pathogenic bacteria can occur. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 140 Figure 5-2-12: Pressing the Start Switch When the buzzer sounds two times - beep, beep, - and "Analyzing" is displayed on the Control Analysis screen, remove the control blood container. Then, automatic analysis is executed. 5-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 141 • To remove the container, lower it straight down. Take care not to bend the sample probe. NOTE: • The sample probe is automatically rinsed, so there is no need to wipe it clean. 5-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 142 Figure 5-2-16: P2. WBC Screen FILE No.1 No.QC01-1 31/12 10:02 × 4.52 /µL 80.1fL RDW-SD 27.4fL RDW-CV 11.4% × /µL 8.3fL 9.1fL P-LCR 14.8% Accept Data? 1:OK 2:NG 3:Print Figure 5-2-17: P3. RBC/PLT Screen 5-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 143 [1] key or [2] key, analysis result cannot be printed. • This print format cannot be changed since it is the special format for QC analysis. 5-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 144 The Analysis Result screen, as in the 1st analysis, can be changed over using [ ] key or [ ] key. The data confirmation message is displayed and you are asked to decide whether or not to accept the analysis result as QC data. 5-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 145 "1: OK" cannot be accepted. Press [HELP] key and perform the error recovery process. For the error recovery process, refer to Chapter 7: TROUBLESHOOTING . 5-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 146 For detail, refer to Chapter 10, Section 5: QC Settings . • As to the host output format, refer to Appendix B: Technical Information . 5-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 147 [Yes]: Rejects analysis result, exists from the QC program, and returns to the Analysis screen. [No]: Returns to the QC Analysis screen and allows continuous QC analysis. Press [ENTER] key to execute the selected process. 5-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 148: Execute L-J Control

    • In analyzing control blood, always wear rubber gloves. After WARNING completion of work, wash the hands with disinfectant. If your hands are contaminated by blood, etc., infection of pathogenic bacteria can occur. 5-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 149 Figure 5-2-27: Pressing the Start Switch When the buzzer sounds two times - beep, beep, - and "Analyzing" is displayed on the L-J Control Analysis screen, remove the control blood container. Then, automatic analysis is executed. 5-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 150 • To remove the container, lower it straight down. Take care not to bend the sample probe. NOTE: • The sample probe is automatically rinsed, so there is no need to wipe it clean. 5-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 151 Figure 5-2-31: P2. WBC Screen FILE No.1 No.QC01-1 31/12 10:02 × 4.52 /µL 80.1fL RDW-SD 27.4fL RDW-CV 11.4% × /µL 8.3fL 9.1fL P-LCR 14.8% Accept Data? 1:OK 2:NG 3:Print Figure 5-2-32: P3. RBC/PLT Screen 5-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 152 172.4 fL [fl] RDW-CV 11.4 % RDW-SD 27.4 fL [fl] 8.3 fL 9.1 fL P-LCR 14.8 % Figure 5-2-34: Example of Analysis Result Printing 5-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 153 56.3 fL W-LMV 172.4 fL RDW-CV 11.4 % RDW-SD 27.4 fL 8.3 fL 9.1 fL P-LCR 14.8 % Figure 5-2-35: Example of Printing 5-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 154: Exit From Qc Control Program

    Press [SELECT] key on the QC Chart screen. The Analysis screen returns. NOTE: • The sample No. and analysis mode on the Analysis screen return to those displayed before execution of the QC program. 5-24 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 155: Deletion

    (option) is incorporated. FILE No.1 (N=30) 31/12 LIMIT DATA (MEAN) 7.1( 7.8) 4.70 4.50 4.52 4.30( 4.55) 15.5 15.0 14.8 14.5( 15.0) 1:Delete 2:Print 3:HC Output Figure 5-3-2: Deletion Menu Screen 5-25 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 156 [Yes]: Deletes QC data of the plot shown by the line cursor and returns the line cursor to the dotted line. [No]: Cancels deletion of QC data and returns the line cursor to the dotted line. Press [ENTER] key to execute the selected process. 5-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 157: Print

    DATA (MEAN) 7.1( 7.8) 4.70 4.50 4.52 4.30( 4.55) 15.5 15.0 14.8 14.5( 15.0) 1:Delete 2:Print 3:HC Output Figure 5-4-2: Print Menu Screen 5-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 158 Printed is the date and time when analysis result was obtained. NOTE: • Printout from the stored QC Chart is only CBC8 parameters. 5-28 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 159: Hc Output (Option)

    Menu Display Area change to the original status. • The line cursor that has changed to solid line cannot be moved. • "3: HC Output" on the menu is displayed only when the serial interface (option) is incorporated. 5-29 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 160 • Data that can be output from the QC Chart are only data of CBC8 parameters. All other data are output with "∗ 0000." For the output format, refer to Appendix B: Technical Information . 5-30 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 161 Reference Values............6-1 Calibration Flow Chart ............. 6-2 AUTOMATIC CALIBRATION..........6-3 Executing Automatic Calibration Program ....... 6-3 Automatic Calibration Procedure.......... 6-4 MANUAL CALIBRATION ............6-9 Calculating Calibration Value..........6-9 Manual Calibration Procedure ..........6-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 162: Chapter 6 Calibration

    This is very important in maintaining the system accuracy. Calibration is carried out by entering calibration values into the unit. Your Sysmex service representative will perform initial calibration of your unit at installation. After installation, the operator should make periodical calibration and proper quality control to maintain the accuracy.

  • Page 163: Calibration Flow Chart

    Enter target value. Page 6-5 Analyze in whole blood Enter calibration value. Page 6-11 Page 6-6 mode. Update calibration value. Update calibration value. Page 6-12 Page 6-8 Completion of HGB/HCT calibration Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 164: Automatic Calibration

    On the Automatic Calibration Menu screen, an automatic calibration parameter can be selected from among "1: HGB," "2: HGB/HCT," and "3: HCT." NOTE: • Each menu can be selected directly by entering a numeral from the numeric keys. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 165: Automatic Calibration Procedure

    Press [SELECT] to Set. Figure 6-2-3: HGB Automatic Calibration Screen * Auto Cal. * Target Data Compensation Current Cal.(%)100.0 100.0 New Cal.(%) Press [SELECT] to Set. Figure 6-2-4: HGB/HCT Automatic Calibration Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 166 • After entering the 5th target value, pressing [ENTER] key does not move the reverse display to the next target column. • An entered value can be set also by pressing [ ] or [ ] key after entering the target value. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 167 52.5 Current Cal.(%)100.0 100.0 New Cal.(%) Figure 6-2-8: Automatic Calibration Screen (Before Analysis) Analyze the samples used for determining the standard value. The sample being analyzed is indicated by the underline cursor. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 168 • If "0" is input to the target value, compensation rate for the sample is not calculated and the analysis result is not used for average compensation rate calculation, either. Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...
  • Page 169 Updates the calibration value and returns to the Analysis screen. [NO] : Does not update the calibration value and returns to the Analysis screen. Press [ENTER] key to execute the selected process. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 170: Manual Calibration

    Previous calibration value of HGB = 100.0% 15.6 100.0 × = 100.65 = 100.7% 15.5 Therefore, the new calibration value of HGB needs to be set at 100.7%. This means that the calibration value increased 0.7%. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 171: Manual Calibration Procedure

    * Manual Cal. * 100.0 100.0 Press [SELECT] to exit. Figure 6-3-2: Manual Calibration Screen NOTE: • Each menu can be selected directly by entering a numeral from the numeric keys. 6-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 172 • An entered value can be set also by pressing [ ] or [ ] key after entering the calibration value. • If no input is made or a space is input, it cannot be set. 6-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 173 [YES]: Updates the calibration value and returns to the Analysis screen. [NO]: Does not update the calibration value and returns to the Analysis screen. Press [ENTER] key to execute the selected process. 6-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...
  • Page 174 Temperature Errors............7-16 Analysis Errors ............7-17 Memory Errors ............7-23 Others..............7-25 Maintenance Errors............7-27 3.10 Built-in Printer Errors............ 7-30 3.11 External Device Errors (When this option is incorporated)..7-33 STATUS DISPLAY............. 7-34 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 175: Chapter 7 Troubleshooting

    When there is not any item that confirms to the trouble in question or if "Action" fails to eliminate the symptom, contact your Sysmex service representative for assistance. When a trouble occurs, the instrument displays an error message on the LCD screen. When there occurs an error unique to a sample analyzed, analysis data is displayed with a flag.

  • Page 176: When You Suspect A Trouble

    TROUBLESHOOTING: 1) Message List To search the pages, the error messages are listed in "Alphabetical" and "Functional" orders. 2) TROUBLESHOOTING Cause and action for each trouble are described for respective error message. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 177: Alphabetical List Of Error Messages Displayed On Analysis Screen

    Rinse Motor Error---------------------------------------------------------------------------7-13, 7-14 Room Temp. High --------------------------------------------------------------------------------- 7-16 Room Temp. Low---------------------------------------------------------------------------------- 7-16 Sampling Error ------------------------------------------------------------------------------7-18, 7-19 Set Value Error ------------------------------------------------------------------------------------- 7-23 Waste Not Draining -------------------------------------------------------------------------------- 7-10 WBC Aperture Clog ------------------------------------------------------------------------------- 7-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 178: Alphabetical List Of Error Messages Displayed On Help Screen

    QC data falls out of control limits. --------------------------------------------------------------- 7-25 Replenish Diluent Container. --------------------------------------------------------------------- 7-11 Replenish Lyse Container. ----------------------------------------------------------------------- 7-12 Room Temp. Error -------------------------------------------------------------------------------- 7-16 Sampling Error has occurred. -------------------------------------------------------------------- 7-19 Waste Not Drained --------------------------------------------------------------------------------- 7-10 WBC/HGB Analysis Error------------------------------------------------------------------------ 7-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 179: Functional List Of Error Messages

    Memory error occurred. (Turn OFF then ON the power.) [Set Value Error] ------ 7-24 Momentary power failure occurred. [-]-------------------------------------------------- 7-24 Others QC data falls out of control limits. [QC Error]------------------------------------------ 7-25 Calibration value is out of range. [Calibration Error] ---------------------------------- 7-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 180 Error on Built-in Printer [Print Error]---------------------------------------------------- 7-30 No Printer Paper [No Printer Paper]----------------------------------------------------- 7-31 Error on Built-in Printer [Printer Error] ------------------------------------------------- 7-32 External Option Errors (When serial interface is incorporated) Host Output Error [Host Comm. Error]------------------------------------------------- 7-33 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 181: Troubleshooting Guide

    If any abnormality is noted, reconnect or replace. After adjustment or check, press [1] key to return to the original screen. Analysis after error Not Ready until the error is resolved. occurrence Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 182 Check the vacuum line for loosened nipple or tube, or breakage. If any abnormality is noted, reconnect or replace. After adjustment or check, press [1] key to return to the original screen. Analysis after error Not Ready until the error is resolved. occurrence Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 183 N O T E : • Press [3] key to return to the Analysis screen. The status is not ready for analysis, but the processing, etc. of stored data in Select Menu can be performed. Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...

  • Page 184: Chamber Errors

    • Press [3] key to return to the Analysis screen. The status is not ready for NOTE: analysis, but the processing, etc. of stored data in Select Menu can be performed. 7-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 185 • Press [3] key to return to the Analysis screen. The status is not ready for NOTE: analysis, but the processing, etc. of stored data in Select Menu can be performed. 7-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 186 • Press [3] key to return to the Analysis screen. The status is not ready for NOTE: analysis, but the processing, etc. of stored data in Select Menu can be performed. 7-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 187: Motor Errors

    • Press [3] key to return to the Analysis screen. The status is not ready for NOTE: analysis, but the processing, etc. of stored data in Select Menu can be performed. 7-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 188 • Press [3] key to return to the Analysis screen. The status is not ready for NOTE: analysis, but the processing, etc. of stored data in Select Menu can be performed. 7-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 189: Transducer Errors

    Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 190: Temperature Errors

    Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 191 Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 192 Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 193 Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 194 Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 195: Analysis Errors

    Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 196 Ready for analysis (although the next sample could be affected) occurrence • Press [3] key to return to the Analysis screen. Although the system is NOTE: ready for analysis, the error may occur in the next analysis. 7-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 197: Memory Errors

    [2] key. Initializing deletes all stored data and QC data, and set values return to initial values. After completion of initializing, perform all settings referring to Chapter 10: INSTRUMENT SETUP. Analysis after error Not Ready until the error is resolved. occurrence 7-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 198 2) Shutdown had not been performed the last time the unit was turned OFF. Action Press [1] key to start up the unit. Perform SHUTDOWN before turning OFF the power. Analysis after error Ready for analysis (This error occurs at starting.) occurrence 7-24 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 199: Others

    When the above 1) and 2) steps fail to resolve the error, a hardware problem may be suspected. Refer to Chapter 4: INSTRUMENT MAINTENANCE AND SUPPLIES REPLACEMENT and clean the transducer, SRV, etc. Analysis after error Ready for analysis occurrence 7-25 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 200 Check QC data, and if data is found to have shifted, a hardware problem is conceivable. Referring to Chapter 4: INSTRUMENT MAINTENANCE AND SUPPLIES REPLACEMENT, clean the transducer, SRV, etc. Analysis after error Not Ready until calibration value is fixed. occurrence 7-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 201: Maintenance Errors

    Analysis after error Ready for analysis occurrence • When you clean SRV without pressing [1] key, execute "7: Maintenance," NOTE: and then "3: Reset Counter" in the Select Menu. 7-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 202 Analysis after error Ready for analysis occurrence • When the waste chamber rinse sequence has been completed, the cycle NOTE: counter is automatically reset. 7-28 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 203 Analysis after error Ready for analysis occurrence • When the transducer rinse sequence has been completed, the cycle NOTE: counter is automatically reset. 7-29 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 204: Built-In Printer Errors

    Chapter 10: INSTRUMENT SETUP and make settings for connecting the built-in printer. Analysis after error Not Ready until the error is resolved. occurrence 7-30 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 205 Chapter 10: INSTRUMENT SETUP and make settings for connecting the built-in printer. Analysis after error Not Ready until the error is resolved. occurrence • When you press [2] key, paper is fed. NOTE: 7-31 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 206 Chapter 10: INSTRUMENT SETUP and make settings for connecting the built-in printer. Analysis after error Not Ready until the error is resolved. occurrence • When you press [2] key, paper is fed. NOTE: 7-32 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 207 To resume transmission to the host computer, refer to Chapter 10: INSTRUMENT SETUP and make settings for connecting the host computer. Analysis after error Not Ready until the error is resolved. occurrence 7-33 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 208 The Status Display screen comprises two pages; you can turn over the pages using [ ] key or [ ] key. P1. Status Display P2. Status Display Screen 1 Screen 2 Figure 7-4-2: Page Selection in Status Display Screen 7-34 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 209 WASTE CHAMBER 2567 DETECTOR 2567 Cycle No. after cleaning transducer 7567 Cycle No. after cleaning SRV Change screen with [ ] or [ ] Figure 7-4-4: P2. Status Display Screen 2 7-35 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 210 INTRODUCTION..............8-1 ADJUSTMENT OF PRESSURE AND VACUUM......8-2 Location of Control Knobs..........8-2 Pressure and Vacuum Display ..........8-3 Adjusting Pressure to 0.5 kg/cm ........8-5 Adjusting Vacuum to 250 mmHg ........8-6 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 211: Introduction

    INTRODUCTION The adjustment procedures described here are important in maintaining accuracy of this instrument. Your Sysmex service representative will perform initial adjustment. Should it require any adjustment subsequently, follow the procedures given below. The following adjustment procedures are explained in this chapter.

  • Page 212: Adjustment Of Pressure And Vacuum

    (Refer to Chapter 7: TROUBLESHOOTING .) * Help * 0.5kg/cm2 Pressure Error 0.35 [0.40 - 0.60] Adjust 0.5kg/cm2 Pressure Restarting to monitor the pressure ERR CODE: XXXX.X.X 1:End of Adj Figure 8-2-2: Help Screen Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 213: Pressure And Vacuum Display

    Change screen with [ ] or [ ] Figure 8-2-4: Status Display Screen NOTE: • Each menu can be selected directly on the Menu screen by entering a numeral from the numeric keys. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 214 1 2 3 4 Change screen with [ ] or [ ] Figure 8-2-5: Status Display Screen Adjust the pressure and vacuum by following the procedures given on the next page and later. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 215: Adjusting Pressure To 0.5 Kg/Cm

    When adjustment is finished, tighten the locking nut while taking care not to allow the adjusting knob to rotate. Press [SELECT] key to return to the Ready status. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 216: Adjusting Vacuum To 250 Mmhg

    When adjustment is finished, tighten the locking nut while taking care not to allow the adjusting knob to rotate. Press [SELECT] key to return to the Ready status. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 217 NAMES AND FUNCTIONS OF INSTRUMENTS ......9-30 8.1 Front Panel..............9-30 8.2 Front Interior .............. 9-31 8.3 Right Side Panel............9-32 8.4 Left Side Panel ............9-33 8.5 Left Side Interior ............9-34 8.6 Rear Panel..............9-35 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 218: Functional Description

    Flow of each analysis parameter on the main unit and analysis method for particle distribution are described. • Function of Measuring Unit: Names and functions of the measuring unit's elements are briefly described. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 219: Detection Principle

    At present, this method cannot be said suitable for a fully-automated instrument which is required to handle a large amount of waste. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 220 Non-cyanide hemoglobin analysis method rapidly converts blood hemoglobin as the Oxyhemoglobin method and contains no poisonous substance, making it suitable for automated method. Being capable of analyzing methemoglobin, this method can accurately analyze control blood, etc. which contain methemoglobin. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 221: Measuring Unit Hydraulic System Block Diagram

    2.08 µL Mixing Chamber (1:25000) 1.922 mL 1.0 mL WBC/HGB Lyse TD Chamber 78 µL (1:1000) HGB Flow Cell (1:1000) 1:26 Diluted Sample (200 µL) Figure 9-3-2: Hydraulic System in Pre-diluted Mode Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 222: Cbc Analysis

    WBC membrane held as they are. At the same time, hemoglobin is converted into red colored methemoglobin. Of the diluted/hemolyzed sample in the WBC transducer chamber, approximately 1 mL is transferred to the HGB flow cell. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 223 (LED) is applied to the sample in the HGB flow cell. Concentration of this sample is measured as absorbance. This absorbance is compared with that of the diluent alone that was measured before addition of the sample, thereby calculating HGB (hemoglobin value). Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 224: Rbc/Plt Analysis Flow

    250 µL of the sample in the RBC/PLT transducer chamber is aspirated through the aperture. At this time, RBC and PLT are counted by the DC detection method. At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection method. Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 225 Of the sample in the RBC/PLT transducer chamber, 250 µL is aspirated through the aperture. At this time, RBC and PLT are calculated by the DC detection method. At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection method. Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 226: Calculation Of Rbc Constant

    HGB (g/dL) ×10 MCH (pg) = RBC (×10 /µL) Mean RBC Hemoglobin Concentration (MCHC) Calculation is made from HCT and HGB by the formula below: HGB (g/dL) ×100 MCHC (g/dL) = HCT (%) Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 227: Blood Cell Discrimination Circuit

    As to PLT LOWER discriminator and UPPER discriminator, the optimum position in 2 - 6 fL and 12 - 30 fL, respectively, are automatically determined by the microcomputer. PLT count is calculated from the particle counts between this LOWER discriminator and UPPER discriminator. 9-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 228: Analysis Of Histogram

    Mixed cells between discriminator (T1) and (T2), which is considered highly correlated with the sum of monocytes, basophils, and eosinophils. NEUT# [W-LCC (WBC-Large Cell Count)] Neutrophils more than discriminator (T2), which is considered highly correlated with neutrophils. 9-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 229 Ratio of lymphocytes to whole WBC MXD% [W-MCR (WBC-Middle Cell Ratio) ] Ratio of mixed cells to whole WBC NEUT% [W-LCR (WBC-Large Cell Ratio) ] Ratio of neutrophils to whole WBC 9-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 230 Probable cause is platelet agglutination, which does not alter WBC count but may result in decreased platelet count. Therefore, this flag is added to the PLT parameter. 9-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1999...
  • Page 231 Table 9-6-1: WBC Histogram Error Flags NOTE: • When analyzing in the pre-diluted mode, since CBC8 parameters only are output, flagging is limited to a WBC parameter. 9-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...
  • Page 232 WBC Histogram Error-WL (1C) +16.4 . [×10 /µL] LYM% ---.- MXD% ---.- NEUT% ---.- LYM# ---.- [×10 /µL] MXD# ---.- [×10 /µL] [fL] NEUT# ---.- [×10 /µL] Figure 9-6-5: WBC Histogram Error-WL (1C) 9-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 233 WBC is flagged with WU and lymphocyte parameters (LYM%, LYM#) are flagged with F1. Mixed cell and neutrophil parameters (MXD%, NEUT%, MXD#, NEUT#) are flagged with T2 and their data are not output. 9-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 234 11) 4B Histogram with high T1 and UD WBC is flagged with WU, lymphocyte parameters (LYM%, LYM#) are flagged with F1, and mixed cell parameters (MXD%, MXD#) with F2. Neutrophil parameters (NEUT%, NEUT#) are not flagged. 9-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 235 15) 5B Histogram with high T2 and high UD WBC is flagged with WU and lymphocyte parameters (LYM%, LYM#) are not flagged. Mixed cell parameters (MXD%, MXD#) are flagged with F2, and neutrophil parameters (NEUT%, NEUT#) with F3. 9-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 236 17) 7 The particle count equal to or less than LD is higher than the range. PLT is flagged with AG and other parameters are not flagged. 9-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...

  • Page 237: Analysis Of Rbc/Plt Histogram

    Figure 9-6-13: Calculation of RDW-CV RDW-SD (RBC Distribution Width - Standard Deviation) is set on 20% frequency level with the peak taken as 100%. • Definition of RDW-SD 100% RDW-SD Figure 9-6-14: Definition of RDW-SD 9-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 238 This is the ratio of large platelets exceeding 12 fL discriminator and is calculated as the ratio of the particle count between the 12-fL fixed discriminator and Upper discriminator (UD) to the particle count between Lower discriminator (LD) and Upper discriminator (UD). 9-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 239 : Any of "O ," "×," and "High" is applicable. Table 9-6-2: RBC Histogram Error Flags NOTE: • Since CBC8 parameters only are output in the pre-diluted mode, a flag is not output to RDW-SD or RDW-CV. 9-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 240 RBC Histogram Error - RU (2A) 3.57 [×10 /µL] 11.5 [g/dL] 41.8 +117.1 [fL] 32.2 [pg] MCHC -27.5 [g/dL] [fL] RDW-SD +91.7 [fL] [×10 /µL] Figure 9-6-17: RBC Histogram Error - RU (2A) 9-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 241 RBC Histogram Error - MP (4) 4.80 [×10 /µL] 14.2 [g/dL] 41.5 86.5 [fL] 29.6 [pg] MCHC -34.2 [g/dL] [fL] RDW- CV +33.3 [×10 /µL] Figure 9-6-19: RBC Histogram Error - MP (4) 9-24 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 242 • In the case of DW, when PDW could not be analyzed • In the case of MP, when PLT histogram has two or more peaks Any of "O ," "×," and "High" is applicable. Table 9-6-3: Platelet Histogram Error Flags 9-25 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 243 MPV and P-LCR are flagged with PU and their data are not output. • Platelet Histogram Error - PU (2B) [×10 /µL] ---.- [fL] ---.- [fL] P-LCR ---.- [fL] Figure 9-6-21: Platelet Histogram Error - PU (2B) 9-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 244 PLT is not flagged. Other parameters are flagged with MP and their data are not output. • Platelet Histogram Error - MP (4) [×10 /µL] ---.- [fL] ---.- [fL] P-LCR ---.- [fL] Figure 9-6-23: Platelet Histogram Error - MP (4) 9-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 245: Electric System

    The beam that has passed through the fluid is detected by the photo diode. And the signals is photoelectrically converted, A/D converted, and then sent to the HGB counting circuit for the calculation of the absorbance. 9-28 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 246 FUNCTIONAL DESCRIPTION Electric Diagram Figure 9-7-1: Electric Diagram 9-29 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 247: Names And Functions Of Instruments

    For detail, refer to Chapter 1, Section 8: GRAPHIC SCREEN. Panel Keyboard This keyboard allows basic operations such as entry of Sample No. and selection of analysis parameters. For detail, refer to Chapter 1, Section 7: PANEL KEYBOARD. 9-30 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 248: Front Interior

    Makes volumetric measurement of aspirated blood. Rinse Cup Cleans the sample probe. WBC/HGB Lyse (STROMATOLYSER-WH) Reagent to measure WBC/HGB Built-in Printer Prints analysis data, error messages, etc. 9-31 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 249: Right Side Panel

    This will overload the fuse and may cause fuse blown. Power Supply Connector Using the furnished power supply cord, this connector supplies power from the outlet. 9-32 Sysmex KX-21 Operator’s Manual -- Revised December 1999 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 250: Left Side Panel

    0.5 kg/cm Regulator Regulates pressure to 0.5 kg/cm 250 mmHg Bellows Unit Regulates vacuum to 250 mmHg vacuum. Air Filter Prevents dirt and dust from entering the bellows. 9-33 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 251: Left Side Interior

    Waste Chamber Collects wastes from transducers and mixing chambers. Pneumatic Unit Supplies pressure and vacuum. CAUTION: • Do no open the left face unless your Sysmex service representative asks you to. 9-34 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 252: Rear Panel

    Connector for communicating with the host computer Drain Outlet Nipple This nipple drains waste. It connects with the drain sewer or waste tank. Diluent Inlet Nipple (CELLPACK) This nipple aspirates diluent. It connects with the diluent container. 9-35 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 253 DATE/TIME SETTINGS ............10-7 PATIENT LIMIT ............... 10-10 QC SETTINGS ..............10-13 HOST SETTINGS (OPTION) ..........10-16 PRINTER SETTINGS............10-21 PRINT SET VALUES ............10-24 PERIPHERAL SETTINGS ........... 10-25 FACTORY SETTINGS............10-27 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 254: Introduction

    INSTRUMENT SETUP INTRODUCTION Your Sysmex service representative performs the settings of the instrument at time of installation. The settings can be changed by using this program. This chapter describes how to use this setting program. System Setup Sets up the instrument system status.

  • Page 255: System Setup

    • "5: Host Settings" is displayed only when the optional serial interface is incorporated. Using [ ] or [ ] key, move the cursor to select "1: System Setup." 10-2 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 256 For General Export Type 5 Standard SI Type 3 Canada SI Type 6 Hong Kong SI Table 10-2-1: Unit Setting For the detail of unit system, refer to the following table: 10-3 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 257 ×10 ×10 ×10 NEUT# ###.# ###.# ###.# RDW-SD ###.# ###.# ###.# RDW-CV #.### #.### ###.# ###.# ###.# ###.# ###.# ###.# ###.# P-LCR #.### #.### ###.# Table 10-2-2: Details of Unit System 10-4 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 258 Settings RDW Calculation Method RDW-SD RDW-SD RDW-CV RDW-CV Table 10-2-5: RDW Setting NOTE: • The settings of system setup become valid from the next time you turn ON the instrument power. 10-5 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 259 (11) Press [ENTER] to execute the selected process. NOTE: • When you change the system setup, always turn OFF then ON the power. Until you do this, the changed contents of the settings will not be reflected. 10-6 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 260: Date/Time Settings

    • "5: Host Settings" is displayed only when the optional serial interface is incorporated. Using [ ] or [ ] key, move the cursor to select "2: Date/Time." 10-7 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 261 12/31/97 dd/mm/yy 31/12/97 Table 10-3-1: Date Format Setting Year Setting Enter in the dominical year using the numeric keys. Month Setting Enter using 1 - 12 from the numeric keys. 10-8 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 262 [Set]: Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. (11) Press [ENTER] key to execute the selected process. 10-9 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 263: Patient Limit

    • "5: Host Settings" is displayed only when the optional serial interface is incorporated. Using [ ] or [ ] key, move the cursor to select "3: Patient Limit." 10-10 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 264 Figure 10-4-3: Scrolling the Screen Using [ ] or [ ] key, move the cursor to select LL (Lower Limit) or UL (Upper Limit). Enter the settings using the numeric keys. 10-11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 265 [Set]: Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. (12) Press [ENTER] key to execute the selected process. 10-12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 266: Qc Settings

    • "5: Host Settings" is displayed only when the optional serial interface is incorporated. Using [ ] or [ ] key, move the cursor to select "4: QC Settings." 10-13 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 267 QC Method Setting Set the QC method. The QC method can be selected from the following two kinds: 17 Settings QC Method X Control L-J Control Table 10-5-1: QC Method Setting 10-14 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 268 Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. (11) Press [ENTER] key to execute the selected process. 10-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 269: Host Settings (Option)

    • "5: Host Settings" is displayed only when the optional serial interface is incorporated. Using [ ] or [ ] key, move the cursor to select "5: Host Settings." 10-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 270 • The settings can also be set when you press [ ] or [ ] key after changing the content of settings. Connect Settings Set whether to connect the host computer or not. Settings Connection Connect Not Use Not Connect Table 10-6-1: Host Output Settings 10-17 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 271 Data Length Settings Set the data length. This can be selected from the following two kinds: Settings Data Length 7 bits 7 bits 8 bits 8 bits Table 10-6-5: Data Length Settings 10-18 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 272 Table 10-6-8: Protocol Settings Transmission interval Settings Select a transmission interval from the following: Settings Interval 0 second 2 seconds 3 seconds 5 seconds 7 seconds 10 seconds 15 seconds Table 10-6-9: Transmission Interval 10-19 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 273 [Set]: Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. (11) Press [ENTER] key to execute the selected process. 10-20 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 274: Printer Settings

    Press [ENTER] key. The current setting status is displayed on the Printer Settings screen. * Printer Settings * Connect Print Condition All Data Print Format Type1 Press [SELECT] to exit. Figure 10-7-2: Printer Settings Screen 10-21 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 275 Data with flags other than Mark Limits • Data with histogram flag • Data out of the linearity limit • For print conditions of QC data, refer to Chapter 10, Section 5: QC • Settings. 10-22 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 276 [Set]: Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. (11) Press [ENTER] key to execute the selected process. 10-23 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 277: Print Set Values

    Press [ENTER] key. The built-in printer starts printing all the set values. NOTE: • If any faulty operation occurs, contact your Sysmex service representative. Keep the printed contents of the settings in good condition because the service representative may have to confirm the contents of settings.

  • Page 278: Peripheral Settings

    Press [ENTER] key. This displays connection status of external devices on the Peripheral Settings screen. * Periph.Settings * Built-in Printer Host Output Not Use Press [SELECT] to exit. Figure 10-9-2: Peripheral Settings Screen 10-25 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 279 [Set]: Updates the settings and returns to the Analysis screen. [Cancel]: Cancels the changed settings and returns to the Analysis screen. Press [ENTER] key to execute the selected process. 10-26 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 280 Auto Output Baud Rate 2400 bps Data Length 7 bits Stop Bit 2 bits Parity Check Even Protocol Class A Interval RTS/CTS Ignore 10-27 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 281 INSTALLATION SPACE ...............A-3 REMOVE SHIPPING CLAMPS ............A-4 CONNECT TUBE ................A-6 Prepare Reagent ..............A-6 Connect CELLPACK..............A-6 Connect STROMATOLYSER-WH..........A-8 Connect Waste Line..............A-9 SET PRINTER PAPER ..............A-10 CONNECT POWER CORD............A-12 TURN POWER ON ..............A-12 Sysmex KX-21 Operator’s Manual -- Revised January 2000...

  • Page 282: Introduction

    INSTALLATION INTRODUCTION This product is a clinical test instrument. Sysmex representative is responsible for unpacking, installing, and initial setup of the product to ensure its proper and safe operation. The next several pages will give some essential information on this instrument.

  • Page 283: Check Before Installation

    INSTALLATION CHECK BEFORE INSTALLATION Make sure the KX-21 is free from external damages and check the quantities of the accessories. Q'ty Part No. Description 117 V 220 V 240 V 461-2263-8 Operator's Manual KX-21 (North American) 461-2261-1 Operator's Manual KX-21 (English) 923-8092-8 Power Cord No.

  • Page 284: Installation Space

    Each dimension of the instrument is shown below. The power cord is 1.8 m long. Width (mm) Depth (mm) Height (mm) Weight (kg) Main Unit 3 5 5 Figure A-3-1: Instrument Dimensions A -3 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 285: Remove Shipping Clamps

    Return the valves and the SRV fixing screw to their original positions. Set the provided sample rotor valve tray. SRV Fixing Screw Tray No. 20 Protection Sheets Figure A-4-2: Removing the Protection Sheets A -4 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 286 Fixing Screws Figure A-4-3: Removing the Screws Remove the rubber caps of the reagent connection nipples. Keep these rubber caps for future use. Rubber Caps Figure A-4-4: Removing the Rubber Caps A -5 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 287: Connect Tube

    No. 1 with the tube polyurethane 4 mmID × 6 mmOD. Set the cubitainer spout kit No. 1 to the CELLPACK container. Cubitainer Spout Kit No. 1 Figure A-5-1: Setting the Cubitainer Spout Kit A -6 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 288 CAUTION: • After connecting the tube, do not pull it by force for reagent replacement. A -7 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 289: Connect Stromatolyser-Wh

    Gently remove the provided float switch No. 23 from the dust-protection bag. Install the float switch No. 23 to STROMATOLYSER-WH. Then, set it to the table. Float switch No. 23 STROMATOLYSER-WH Figure A-5-4: Installing the STROMATOLYSER-WH A -8 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 290: Connect Waste Line

    Arrange and tie the diluent and waste line tubings as shown in the figure using the provided clamp. Diluent Inlet Nipple Drain Outlet Nipple Tube Polyurethane 6 mmID × 9 mmOD Waste Sewer Waste Tank Clamp Figure A-5-5: Connecting the Waste Line and Clamping Tubings A -9 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 291: Set Printer Paper

    Turn the release lever up to free the paper. Release Lever Figure A-6-1: Releasing the Lever Mount a printer paper. Printer Paper Figure A-6-2: Mounting a Printer Paper A -10 Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised January 2000...
  • Page 292 Figure A-6-3: Securing the Release Lever Cut off any printer paper extending from the upper part of the printer. Figure A-6-4: Cutting Off Paper Close the front cover of the main unit. A -11 Sysmex KX-21 Operator’s Manual -- Revised October 1998...

  • Page 293: Connect Power Cord

    Figure A-7-1: Connecting the Power Cord TURN POWER ON It is necessary to run the service sequence at the initial start-up. Your Sysmex service representative is responsible for the operation. A -12 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 294 TECHNICAL INFORMATION APPENDIX B: TECHNICAL INFORMATION OUTPUT FORMAT FOR HOST COMPUTER (OPTION)......B-1 Hardware................B-1 Software................B-3 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 295 The bit serial voltage type, which conforms to the RS-232C interface, is used for host computer output of the KX-21. The serial interface port for the connection with the host computer is on the rear panel of the main unit, when an option is incorporated.
  • Page 296 Interface Circuit • Output circuit 300 Ω EMI filter MC145407 Driver Figure B-1-1: Interface Output Circuit • Input circuit 15 kΩ EMI filter 5.4 kΩ MC145407 Receiver Figure B-1-2: Interface Input Circuit Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 297 • Class A One-way transmission to the host computer without requiring ACK nor NAK from host computer. • Class B The KX-21 transmits data and then waits for ACK or NAK to complete the data transmission. KX-21 Host computer Analysis data is received.
  • Page 298 • After the data is transmitted, other than ACK or NAK is sent from the computer. (Class B only) • After the data is transmitted, the fourth NAK is sent. (Class B only. The KX-21 resends the data automatically up to 3 times when NAK is sent.)
  • Page 299 [×10 /µL] XXX.XF NEUT# (W-LCC) [×10 /µL] XXX.XF RDW-SD/CV [fL/%] XXX.XF [fL] XXX.XF [fL] XXX.XF P-LCR XXX.XF (03 H) Table B-1-4: Analysis Data Format Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised February 1999...
  • Page 300 [fL] XXX.X [pg] XXX.X MCHC [g/dL] XXX.X RDW-(CV/SD) [%/fL] XXX.X [×10 /µL] XXXX [fL] XXX.X [fL] XXX.X P-LCR XXX.X W-SMV [fL] XXX.X W-LMV [fL] XXX.X (03 H) Table B-1-5: QC data format Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 301 RBC histogram information RBC histogram flag PLT histogram information PLT histogram flag Table B-1-7: PDA Information Code Description "0" Histogram is normal. "1" Histogram is abnormal. " "2 Manually discriminated Table B-1-8: Histogram Information Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 302 Particle distribution Flagging characters of LCD and built-in printer FLAG “0” “1” “2” “3” “4” “5” “6” “7” “8” “9” "A" Table B-1-10: Flagging Character Sysmex KX-21 Operator’s Manual -- Revised October 1998 Sysmex KX-21 Operator’s Manual -- Revised December 1998...
  • Page 303 If the data over-flows, it is indicated as follows: *0003 10) In case of analysis error, or if the data lacks some data such as the calculation parameter in pre-diluted mode, the data is transmitted as follows: *0000 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 304 (Shutdown) ------------------------------4-4 Functional List of Error Messages------------7-5 Clean Transducer (Rinse Sequence)-------- 4-12 Clean Waste Chamber (Rinse Sequence)-----4-9 Graphic Screen---------------------------------1-7 Clean WBC/RBC Transducer Aperture ----- 4-28 Grounding------------------------------------ 1-12 Clog in the aperture-------------------------- 7-15 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 305 No Printer Paper ----------------------------- 7-31 Replace Fuse---------------------------------- 4-36 Non-Cyanide Hemoglobin Analysis Method9-2 Replace Printer Paper------------------------ 4-37 Replace Waste Tank-------------------------- 4-32 Replenish Diluent --------------------- 4-33, 7-11 Replenish Lyse ------------------------ 4-33, 7-12 Replenish Reagent --------------------------- 4-33 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
  • Page 306 Troubleshooting Guide (Motor Errors)---- 7-13 Troubleshooting Guide (Others) ----------- 7-25 Troubleshooting Guide (Pressure/Vacuum Errors)--------------7-7 Troubleshooting Guide (Temperature Errors)----------------- 7-16 Troubleshooting Guide (Transducer Errors)------------------- 7-15 Turn Power On-------------------------------A-12 Turning ON the Power and Self-Check------2-5 Sysmex KX-21 Operator’s Manual -- Revised October 1998...
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