SYSMEX CyFlow Cube 8 Operating Manual
  1. Manuals
  2. Brands
  3. SYSMEX Manuals
  4. Medical Equipment
  5. CyFlow Cube 8
  6. Operating manual
SYSMEX CyFlow Cube 8 Operating Manual

SYSMEX CyFlow Cube 8 Operating Manual

Hide thumbs Also See for CyFlow Cube 8:
Table of Contents

Advertisement

Quick Links

®
CyFlow
Cube 8
Operating Manual
For Research Use Only. Not for use in diagnostic procedures.
®
CyFlow
Software Version 1.0 RUO
Doc. No.: CY-S-3068RIFUEN | Doc. Rev.: 032 | 17-05-2018 | EN | CN 383

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the CyFlow Cube 8 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for SYSMEX CyFlow Cube 8

Summary of Contents for SYSMEX CyFlow Cube 8

  • Page 1 ® CyFlow Cube 8 Operating Manual For Research Use Only. Not for use in diagnostic procedures. ® CyFlow Software Version 1.0 RUO Doc. No.: CY-S-3068RIFUEN | Doc. Rev.: 032 | 17-05-2018 | EN | CN 383...
  • Page 2 D-02828 Görlitz Germany www.sysmex-partec.com All rights reserved. No part of this manual may be reproduced, transcribed or translated in any form or by any means electronic or otherwise, without the prior permission of Sysmex Partec GmbH. ® CyFlow Cube 8 | Operating Manual | May 2018 | Revision 032...

  • Page 3: Table Of Contents

    Table of contents Table of contents 1 Introduction ..................... 6 About this operating manual ............ 6 Typographical conventions ............7 General conditions ..............8 CE compliance ................8 2 Safety ....................... 9 Intended use................9 Prohibited use ................9 Reasonably foreseeable misuse ..........9 User definition................
  • Page 4 Table of contents 6 Operation ....................24 Inserting the Software dongle..........24 Start-up procedure..............24 6.2.1 Switching on the device .............24 6.2.2 Logging in and logging out ............25 6.2.3 Performing a Prime ..............26 Performing a measurement.............27 Performing an intermediate cleaning........28 Shutdown procedure ...............29 6.5.1 Cleaning for shutdown ...............29 6.5.2...
  • Page 5 Table of contents 12 Software description ................50 12.1 Overview ................... 50 12.2 Views of the software window ..........52 12.3 Quick Access Toolbar ............. 56 12.4 Ribbon..................58 12.4.1 File tab ..................58 12.4.2 Home tab ................... 65 12.4.3 Analyze tab ................68 12.4.4 Preview tab ................

  • Page 6: Introduction

    There are product data sheets for the reagent kits and application notes available. Please also refer to these documents. If you have any questions about the content of this operating manual or the use of the device, contact your local Sysmex representative. ® CyFlow...

  • Page 7: Typographical Conventions

    1 Introduction 1.2 Typographical conventions In this operating manual, warnings are indicated by a signal word. Hazards are categorised into hazard levels with consequences of differing severity. Type of hazard This will result in death or have a serious impact on health. Information about avoiding the hazard.

  • Page 8: General Conditions

    This operating manual must be read prior to using the device.  Report any recurring faults or problems to your local Sysmex  representative. Sysmex Partec GmbH does not accept liability for any damage or personal injury arising from: Modifications or manipulation of the device;  Improper use;...

  • Page 9: Safety

    2.2 Prohibited use Never use the device with organic solvents as this can destroy the fluidic system. Only use particles with a diameter < 100 µm. Only use Sysmex approved fluids for sheath, cleaning and decontamination. Never for use in diagnostic procedures.

  • Page 10: User Definition

    Instructed personnel have to be trained by qualified personnel or personnel authorised by Sysmex to operate the device within the scope of the intended use. They have to be able to assess if the device can be safely used and are proficient in handling hazardous substances.

  • Page 11: User Qualification Table

    2 Safety 2.4.2 User qualification table Activity Instructed Qualified Authorised personnel personnel service personnel Transport × × Installation × Operation × × × Cleaning × × × Decontamination × × Maintenance ×¹ ×¹ × Technical service × Uninstalling × × Packing ×...

  • Page 12: Prohibition And Warning Signs On The Device

    2 Safety 2.6 Prohibition and warning signs on the device The following prohibition and warning signs have been affixed to the device. Warning sticker Meaning Laser radiation Electrical hazards Beware of electrical currents and electrical shocks Additional laser labelling 2.7 Personal protective equipment The following personal protective equipment is recommended to operate the device.

  • Page 13: Laser Safety

    2 Safety 2.8 Laser safety The device is equipped with a class IIIb laser unit. If the housing of the device is closed, the laser class of the laser unit is classified as a laser class I (EN 60825-1:2015). Never open the housing of the device. Laser light of laser class IIIb can be emitted if the housing of the device is damaged or the laser protection is removed.

  • Page 14: Overview

    3 Overview 3 Overview ® Your CyFlow Cube device is a fully equipped desktop Flow Cytometer (FCM). Which features a modular optical concept. This allows different lasers with different wavelength ranges be used as light sources and the detection of up to 8 optical channels (parameters). The device allows the optics to be optimised for any application simply by exchanging optical filters and mirrors.
  • Page 15 3 Overview Fig. 3.2 Ports for external devices 1 USB port 9 USB port 2 HDMI port 10 USB port 3 VGA port 11 USB port 4 Ethernet port 12 DVI port 5 USB port 13 USB port 6 Ethernet port 14 Keyboard port 7 Audio port 15 Mouse port...
  • Page 16 3 Overview Fig. 3.4 Sample port at the right side of the device 1 Start electrode 4 Sample holder 2 Stop electrode 5 Sample port injection needle 3 Sample tube ® CyFlow Cube 8 | Operating Manual | May 2018 | Revision 032...

  • Page 17: Components Of The Device

    3 Overview 3.1 Components of the device The device contains the flow cuvette, the optics, the fluidics, the computer unit and data acquisition electronics. No work steps inside the device are necessary. The main components of the device are: Control elements The device is operated via keyboard and mouse.

  • Page 18: Type Plate

    3 Overview 3.2 Type plate The type plate is attached to the rear side of the device. The content of the type plate varies according to the system configuration. The system configuration is represented by an addition to the REF number (e.g.

  • Page 19: Scope Of Delivery

    3 Overview 3.3 Scope of delivery Fig. 3.5 Scope of operation ® 1 CyFlow Cube device 3 Waste bottle 2 Keyboard and mouse 4 Sheath fluid bottle with inline filter for sheath fluid The scope of delivery includes: ® 1 CyFlow Cube device;...
  • Page 20 3 Overview 1 Blue cap with electrodes, cabling and tubing for sheath including inline  filter; 1 Red cap with electrodes, cabling and tubing for waste.  The scope of operation includes: ® 1 CyFlow Cube device;  1 Power cord; ...

  • Page 21: Accessories And Spare Parts

    Robby 8 Autoloading Station  ® CyFlow® Sorter for CyFlow Cube  Visit our website http://www.sysmex-partec.com or contact your local Sysmex representative for further information about our products. ® CyFlow Cube 8 | Operating Manual | May 2018 | Revision 032...

  • Page 22: Transport And Storage

    4 Transport and storage 4 Transport and storage Warning! The weight of the device is about 40 kg. Always have at least two people carry or lift the device. In order to transport the device to a different location all external data and supply connections have to be disconnected.

  • Page 23: Installation

    5 Installation 5 Installation 5.1 Positioning the device Heavy weight When moving the device (packed or unpacked) or removing it from its packaging, a person is exposed to a high risk of damaging his or her body due to the heavy weight of the device. At least two persons are required for transport and installation.

  • Page 24: Operation

    6 Operation 6 Operation Due to heavy use of software-related content in the following chapter please also refer to chapter 12 Software description on page 50. 6.1 Inserting the Software dongle Procedure 1. Insert the included Software dongle into an open USB port of the device. Result The software will check automatically in regular intervals if the dongle is ...

  • Page 25: Logging In And Logging Out

    6 Operation ® 4. Start the software by double click the CyFlow Software icon. – The log-in dialogue is displayed. – The device is, by default, set in a standard mode. 6.2.2 Logging in and logging out Each user is assigned to a user role. Each user role has specific privileges. Some functions of the software are not available for each user role and availability depends on the assigned privileges.

  • Page 26: Performing A Prime

    6 Operation 4. Select the respective user role. 5. Click the OK button. – If the log-in was successful the start screen is displayed. After a specified number of unsuccessful login attempts (default 3), the account will be locked. If the account is locked, please contact a user with the "user administration"...

  • Page 27: Performing A Measurement

    6 Operation 6.3 Performing a measurement Requirements The device is primed.  The respective Worklist is loaded.  A Worklist item is active with a defined Workspace.  The Worklist contains at least one defined item that does not contain a ...

  • Page 28: Performing An Intermediate Cleaning

    6 Operation 6.4 Performing an intermediate cleaning A cleaning mode is available to clean the device when a different sample type is to be measured. Requirements Log-in as a user with the respective role.  The respective Worklist is loaded. ...

  • Page 29: Shutdown Procedure

    6 Operation 6.5 Shutdown procedure 6.5.1 Cleaning for shutdown Perform the shutdown cleaning procedure to close the software. Requirements No acquisition is in progress.  Sample tube with 1.6 ml Decontamination Solution (Order No.  04-4010_R) connected. Procedure 1. On the File tab of the Ribbon (see chapter 12.4.1 File tab on page 58) click the Exit CyFlow Software button.

  • Page 30: Software Operation

    6 Operation 6.6 Software operation The software can be run in two different modes: In Worklist mode, data can be acquired. The hardware and software  settings for each measurement are predefined and organised in a list called Worklist. In Playlist mode, acquired data can be analysed and settings for future ...
  • Page 31 6 Operation Creating a new Worklist Requirements To create a Worklist for future measurements, login as an assay  developer is necessary. Procedure 1. On the File tab of the Ribbon (see chapter 12.4.1 File tab on page 58) select "File > New > New Worklist". In Worklist mode, a new Worklist can also be created by clicking the New button in the Worklist pane (see chapter 12.6 Worklist pane on page 146).

  • Page 32: Cyflow ® Cube 8 | Operating Manual | May 2018 | Revision

    6 Operation Presetting instrument settings Procedure 1. Select the Settings pane (see chapter 12.5.3 Settings pane on page 139). 2. To select a parameter as trigger parameter, set a parameter to "ON" in the "Logic" column (only available if all other parameters are set to "OFF").
  • Page 33 6 Operation Adapting instrument settings during data acquisition Procedure 1. Select the Settings pane (see chapter 12.5.3 Settings pane on page 139). 2. In the "Voltage" column, adapt the voltage until the desired cell populations are displayed in the proper range in the plots and can be clearly separated.
  • Page 34 6 Operation the "Region Name" box to enter a name or to select a name from the drop-down list. – A region is created. Setting a parent gate A region that contains all events of interest can be applied as a parent gate to the other plots so that only the relevant events are displayed on these plots.
  • Page 35 6 Operation Performing a manual compensation A manual compensation can be performed online (during the measurement) or offline (after data acquisition). Procedure 1. On the Compensation tab of the Ribbon (see chapter 12.4.6 Compensation tab on page 84) click the Manual Compensation button and the Plot Compensation button.
  • Page 36 6 Operation Defining the Worklist for remaining samples Defining a Worklist for future measurements includes defining the Workspace, instrument settings and stop conditions. Instrument settings and stop conditions are loaded from the Workspace by default but can be changed in the Worklist. Procedure 1.

  • Page 37: Analysing Data And Creating Reports Using The Playlist

    6 Operation 6.6.2 Analysing data and creating reports using the Playlist Data can be analysed offline by using a Playlist (see chapter 12.7 Playlist pane on page 154). All data created for analyses, including plots, statistics, compensation matrices and overlays, can be summarised in a page-based report that can be printed.
  • Page 38 6 Operation Creating a report A report can be created manually in the Reports tab of the Workspace (see chapter 12.8.4 Reports tab of the Workspace on page 179). Alternatively, in Playlist mode, the Report Wizard from the Report tab of the Ribbon can be used (see chapter 12.4.12 Report tab on page 114).

  • Page 39: Troubleshooting

    – After successful de-bubble process the device is ready for the sample measurements. – If the de-bubble process was not successful, please contact your local Sysmex representative. 7.2 Performing a de-clog procedure Requirements Log-in as a user with the respective role.

  • Page 40: Sheath Fluid, Waste And Fluids

    7 Troubleshooting 7.3 Sheath fluid, waste and fluids Observation Possible solution Sample flow is very Check that the waste bottle is closed properly and slow or is not that the bottle is not cracked. running. Check all visible tubes and make sure that they are not pinched.

  • Page 41: Calibration And Count Check Beads (Low, Medium, High)

    7 Troubleshooting 7.4 Calibration and Count Check Beads (low, medium, high) Observation Possible solution The results of the Shake the bottle vigorously (e.g. by vortexing) and Count Check Beads repeat the measurement. (low, medium, high) Check the date of expiry of the Count Check Beads measurements are (low, medium, high).

  • Page 42: Cleaning

    8 Cleaning 8 Cleaning Contaminated sheath fluid bottle A contaminated sheath fluid bottle disturbs the proper operation. Clean the sheath fluid bottle regularly.  Using incorrect cleaning solvents Using incorrect cleaning solvents when cleaning the casing and screen could damage the device. Do not use any organic solvents, nitro thinner, benzol, alcohol or highly ...

  • Page 43: Performing A Cleaning Procedure

    8 Cleaning 8.2 Performing a cleaning procedure The device can be cleaned between sets of samples using a Cleaning Solution (Order No 04-4009_R) followed by a sample tube with sheath fluid. This procedure will allow you to significantly reduce cross contamination and reduce the background.

  • Page 44: Maintenance

    9 Maintenance 9 Maintenance Servicing is to be performed by an authorised service engineer. Please contact your local Sysmex representative for further instructions. Sequence Activity Minimum user qualifi- cation level Daily Prime the device by using the Prime Instructed maintenance function (see chapter 6.2.3 Performing a...

  • Page 45: Disposal

    Electrical and electronic equipment must be disposed of separately from normal waste. Dispose of this product by taking it to your local collection point or recycling centre for such equipment. This will help to protect the environment. For further information, please contact your local Sysmex representative. 10.1 ® CyFlow...

  • Page 46: Technical Data

    11 Technical data 11 Technical data Heavy weight When moving the device (packed or unpacked) or removing it from its packaging, a person is exposed to a high risk of damaging his or her body due to the heavy weight of the device. At least two persons are required for transport and installation.
  • Page 47 11 Technical data Hardware data Operating Temperature: environment Operation: 15 °C to 30 °C Transport and storage: 5 °C to 50 °C Relative humidity: Operation: 20 % to 85 %, non-condensing Transport and storage: 20 % to 85 %, non-condensing Room: Clean environment.
  • Page 48 11 Technical data Optics (dependent on system configuration) Laser output V1: Blue laser 488 nm 50 mW V2: Blue laser 488 nm 50 mW, Red laser 638 nm 25 mW V4: Blue laser 488 nm 50 mW, Red laser 638 nm 25 mW, UV-LED 365 nm V5: Violet laser 405 nm 100 mW, Blue laser 488 nm 50 mW, Red laser 638 nm 25 mW...
  • Page 49 11 Technical data Fluids Fluid system Completely closed system for sheath fluid and sample volumes; no fluid droplets or aerosols generated nor released from the device Flow cuvette Synthetic quartz flow cuvette with small centric flow channel (capillary diameter 350 µm x 250 µm) for laminar sample transport with sheath fluid.

  • Page 50: Software Description

    12 Software description 12 Software description ® The device is operated by means of the CyFlow software. This chapter describes the software functions. Performing routine tasks using the software is described in chapter 6.6 Software operation on page 30. 12.1 Overview Data acquisition, device control and data analysis are controlled and performed by the integrated software.
  • Page 51 12 Software description The default view of the software window contains the following elements: Quick Access Toolbar: The Quick Access Toolbar provides quick  access to some of the most commonly used options in the software. Ribbon: The Ribbon provides access to the main functionality groups of ...

  • Page 52: Views Of The Software Window

    12 Software description 12.2 Views of the software window The software window view can be adapted by displaying or hiding the panes and by docking and undocking the panes. Undocked panes can be positioned and docked as required. The view settings are persisted between sessions. Displaying and hiding the panes By default, the panes are open and pinned to the left-hand side of the software window.
  • Page 53 12 Software description 1 Tabs of the panes To re-expand a pane, click the desired tab. The pane is visible as long as the cursor is over the pane. To refix the panes in this open position so they are always visible, click the Auto Hide button on the expanded pane.
  • Page 54 12 Software description Undocking and positioning the panes The panes, including the Worklist pane and the Playlist pane, can be positioned and docked as required. To undock a pane, the following options are available: Click the title bar and relocate the pane by dragging and dropping. When ...
  • Page 55 12 Software description The Worklist pane can be docked and nested with the other panes by dragging it and placing the cursor in the centre of the relocation arrows on the left-hand side of the software window. 1 Centre of the relocation arrows The size of docked panes can be adjusted by dragging the splitter bar to the desired position.

  • Page 56: Quick Access Toolbar

    12 Software description 12.3 Quick Access Toolbar The Quick Access Toolbar provides quick access to some of the most commonly used options in the software. By default, the Quick Access Toolbar contains an Open Listmode button, an Undo button and a Redo button. You can customise the Quick Access Toolbar.
  • Page 57 12 Software description Minimising the Ribbon Usually the Ribbon is fully visible. But if you need more place on the display, you have the possibility to minimise the Ribbon. Then, only the tabs are visible. Check or uncheck the Minimize the Ribbon menu item in the drop-down list to toggle the Ribbon between minimised and restored.

  • Page 58: Ribbon

    12 Software description 12.4 Ribbon The Ribbon is the area at the top of the software window that presents an easy-to-browse main set of commands. The Ribbon is organised into a set of tabs which represent the main functionality groups of the software. 12.4.1 File tab The File tab contains file open, printing and export options.
  • Page 59 12 Software description Open The Open menu contains the following options: Open Worklist…: Click the menu item to open a Worklist file (XMLWL  file). Only one Worklist file can be open at a time. Open Listmode…: This menu item is only displayed in Playlist mode. ...
  • Page 60 12 Software description In Playlist mode, the Save menu contains the following options: Save Workspace: Click the menu item to save the current Workspace  with the current Workspace file name. A Workspace includes plots, regions, gates, colour precedence, selected statistics and displayed parameters.
  • Page 61 12 Software description Export The Export menu is only available in Playlist mode except for the Export Report to PDF menu item which is also accessible in Worklist mode. The menu contains the following options: Export Statistics to CSV: This menu item is only enabled when there ...
  • Page 62 12 Software description If the original Playlist (before export) contains the same LMD file in more than one of its items, only one copy of the file is in the export LMD folder. If the original Playlist (before export) contains LMD files that have the same name but are in different folders, they are all placed in the exported LMD folder with numbers in parenthesis appended to the file name, so they can be distinguished.
  • Page 63 12 Software description When in Worklist mode, reports are exported by panels. If a Worklist contains several panels, a corresponding message is displayed and you will have the option to export only the current panel report or all panel reports. On exporting, the software creates a subfolder inside the destination folder with a name containing the Worklist name and a time stamp.
  • Page 64 12 Software description Accessible: Click the button to restore access for the selected user.  Access is locked when a user enters a defined number of incorrect passwords consecutively. The number is defined in the "Log In Attempts" item. Password: Click the button to enter a temporary password for the ...

  • Page 65: Home Tab

    12 Software description 12.4.2 Home tab The Home tab is the default tab and provides all the essential controls for analysing files in one convenient place. Here, files can be selected, regions created and plots created and gated. Load group The Load group contains controls for opening listmode files and Workspaces.
  • Page 66 12 Software description Linear Region: Click the button to preselect creating a linear region on a  single parameter plot. Quadrant Region: Click the button to preselect creating a quadrant  region on a dual parameter plot. Region Color: The button is only enabled if a region is selected on a ...
  • Page 67 12 Software description The Parameters group contains the following options: X and Y Parameter Selection:  The X and Y parameter for the currently selected plots can be set using these boxes. Click the arrow to expand a drop-down list and select the desired parameter.

  • Page 68: Analyze Tab

    12 Software description 12.4.3 Analyze tab The Analyze tab provides controls for analysing and modifying the plots in the Plots area of the Workspace. Region group The Region group contains options for creating and editing regions. You can create a maximum of 256 regions per Workspace. Once this number is reached, the region creation buttons in the Region group become disabled.
  • Page 69 12 Software description Upper Triangle: Click the button to add all the dual parameter plots that  are displayed within the upper right triangle of the Previews area. All the added plots are colour-coded to illustrate the gating applied to the Previews area.
  • Page 70 12 Software description In the Precedence Order dialogue, a gates list displays all gates in the current Workspace. The text colour corresponds to their gate colour. Adjacent to each gate name is a check box. Select the check boxes to include the corresponding gates in the colour precedence.
  • Page 71 12 Software description Using the Edit Gates button, you can create, edit and delete gates. In addition, you can view the Parent Gate and Expanded Equation information. The Edit Gates dialogue displays a Gates list containing all the currently existing gates. In the Gates tab, you can rename and delete gates. In the Derived Gates tab, you can rename, delete and change the gate equation of derived gates.
  • Page 72 12 Software description In the Derived Gates tab, the Gates list contains the following columns: The "Gate name" column displays the name of the gate. The colour of  the font is the colour of the gate. The "Equation" column displays the equation for the gate. The contents ...
  • Page 73 12 Software description View group The View group contains options to modify the way in which plots are displayed in the Plots area of the Workspace. The View group contains the following options: Gallery: Click the button to choose the Gallery plot view. The plots in the ...

  • Page 74: Preview Tab

    12 Software description When on the Report tab of the Workspace, click the Copy button to copy the selected report objects for pasting into the report. Report items cannot be pasted into other applications. Delete: Click the button to delete the currently selected plots or region. If ...
  • Page 75 12 Software description switches between hiding and showing the previews. The setting of this button will be persisted between sessions. Previews Topmost: By default, the Plots area is positioned above the  Previews area in the Workspace. Clicking the button switches between displaying the Previews area above and below the Plots area.

  • Page 76: Parameters Tab

    12 Software description Display Zoomed: If zoom levels are set for individual parameters, click  the button to select or deselect displaying those parameter zoom settings in the Previews area. The default is to display parameters in the Previews area zoomed. For details on setting parameter zoom values, see chapter Scales Properties dialogue on page 81.
  • Page 77 12 Software description The Parameter Properties dialogue contains two tabs: On the Customize tab, you can select which parameters are displayed in  the Previews area. You can also choose a display name and assign custom names to individual parameters. On the Classification tab, you can re-classify the group and type of a ...
  • Page 78 12 Software description The "Displayed Label" column displays the name as selected in the  "Display Name" column. In the "Displayed Label" column, you can also enter a custom label for the parameter. The default used for the custom name is the Stain name ($PnS). The custom name has to be unique. It cannot start with a space, and it has to be a minimum of 1 character and can be a maximum of 50 characters.
  • Page 79 12 Software description The "On" column allows you to select whether the parameter belongs in  the Height (Peak), Width (Time of Flight), Area (Integral) or Other type. Double-click in the cell or press F2 to open a drop-down list and select the desired type for the parameter.
  • Page 80 12 Software description Hovering the cursor over a check box will open a tooltip containing the display name followed by the stain name for the parameter if a stain name is available. The Scatter group lists the scatter parameters, forward scatter (FSC) and side scatter (SSC).
  • Page 81 12 Software description The Zoom tool allows you to modify the zoom level for parameters of a  plot. To set or change a zoom level for parameters of the selected plot, select the tool and then use it to define a zoom region. On a single parameter plot, the tool draws a linear zoom region.
  • Page 82 12 Software description The Default Scaling tab provides the following options: Full Range: Select this option to scale all linear parameters in the  Workspace and reports to the full range of data. 1024 Channels: Select this option to scale all linear parameters in the ...
  • Page 83 12 Software description Enter the desired value or use the arrows to increase or decrease the value in the field. For example, when set at 100%, the number of decades displayed on the negative section of the scale will be the same as the number of decades displayed on the positive side of the scale.

  • Page 84: Compensation Tab

    12 Software description The Parameter Zoom tab contains the following columns: The "Parameter" column contains the display name for each parameter in  the listmode file. This column has a tooltip detailing the acceptable range for the Scale Min and Scale Max with respect to the scale type selected. The "Scale Type"...
  • Page 85 12 Software description FL2, FL3, etc. You will be able to reallocate files later if required. If a single LMD file contains more than one positive stain, you must load in the file once for each stain present. The Wizard will allocate files based on the Playlist starting from the first file.
  • Page 86 12 Software description Select the parameters for the gating plot: Select the parameters for  the primary bead / cell gating plot using the "X parameter" and "Y parameter" boxes. They are populated with all the parameters shown in the "Parameters" list. Select background fluorescence mode: If no background fluorescence ...
  • Page 87 12 Software description Compensation Wizard: Background Fluorescent Page The Background Fluorescence page of the Compensation Wizard is only displayed if the "Use negative control file" option is selected on the Configuration page. On this page, you will define a default gate position for subsequent pages and then use a region to define the negative events for each parameter.
  • Page 88 12 Software description Compensation Wizard: Compensation Pages A Compensation page will be displayed once for each selected compensation parameter. The page contains the following options: Stain for compensation: The field displays the stain that is being  currently compensated. Stain listmode file: The field displays the name of the listmode file used ...
  • Page 89 12 Software description Back: Selecting the button returns you to the previous page of the  Compensation Wizard. The Back button on the first Compensation page is disabled if the "Use negative control file" option was not selected. Next: Selecting the button proceeds to the next Compensation page or to ...
  • Page 90 12 Software description The Plot Compensation group contains the following options: Plot Compensation: To enable plot compensation, select a plot in the  Plots area that contains the parameters required for compensation and then click the button. The button is highlighted, and the arrow buttons and manual dragging of populations on plots in the Plots area are enabled.
  • Page 91 12 Software description Click the Mean/Median button to select whether the mean or median statistic is displayed for the quadrant region (default: "Median"). Click the X Stats/Y Stats button to select whether the displayed statistics are for the X parameter or the Y parameter (default: "X Stats"). Preview Details group Controls in the Preview Details group contains options to manually adjust the compensation of the current sample using the preview plots and also to...

  • Page 92: Statistics Tab

    12 Software description fit, the printout of the matrix is cropped, and a corresponding message is displayed at the end of printing. Click the Size to Fit button to resize the Compensation Matrix dialogue to fit the size of the current matrix. This is restricted by the screen size of your monitor.
  • Page 93 12 Software description Results Tables group The Results Tables group provides options for working with tables on the Results tab of the Workspace. The Results Table group contains the following options: Add Table: Click the button to create a new results table. ...
  • Page 94 12 Software description Region displays the name of the region or the DNA plot name.  Filename displays the LMD file name.  File Path displays the LMD full path and file name.  Plot Title displays the title of the plot. ...
  • Page 95 12 Software description X Mean displays the mean average for the X-axis histogram of a region.  Y Mean displays the mean average for the Y-axis histogram of a region.  Where the region is only a single parameter region, the entry on the results table is left blank.
  • Page 96 12 Software description DNA Statistics group The DNA Statistics group displays the statistical options associated with the DNA cell cycle plots. The items selected in this group are displayed in the results table and in selected Stats Boxes on the reports. When a cell cycle plot is present in the Plots area, the statistics in the DNA Statistics group are selected by default.

  • Page 97: Worklist Tab

    12 Software description 12.4.8 Worklist tab The Worklist tab provides controls for modification of all aspects of a Worklist including Worklist items, panels, Workspace files and instrument settings. The Worklist tab is only available in Worklist mode. General group The General group contains options to open, save and edit a Worklist. The General group contains the following buttons: New Worklist: Click the button to create a new Worklist ...
  • Page 98 12 Software description Panel group The Panel group contains options to edit a panel of the Worklist. The Panel group contains the following buttons: Save: Click the button to save the panel with the same name.  Save as: Click the button to save the panel with a new name. ...
  • Page 99 12 Software description Instrument Settings group The Instrument Settings group contains options to save or replace instrument settings files. The Instrument Settings group contains the following buttons: Save: Click the button to save the instrument settings file with the same ...

  • Page 100: Playlist Tab

    12 Software description 12.4.9 Playlist tab The Playlist tab provides controls for modification of all aspects of a Playlist including Playlist items, Playlist files, Workspace files and compensation files. The Playlist tab is only available in Playlist mode. Listmode group The Listmode group contains options to modify the listmode files in the Playlist.
  • Page 101 12 Software description Scaling Mismatch: A mismatch between listmode and Workspace scale  can occur. In this case, the LMD file is replaced, and the Workspace is modified to match the LMD file settings. Any zoom settings that have been applied are reset. A corresponding message is displayed as soon as the LMD file is opened in the Workspace.
  • Page 102 12 Software description Clear: The button is enabled when one or more Playlist items are  selected in the Playlist. Click the button to replace the Workspace files in the selected items with the default blank Workspace. The listmode and compensation files are not affected.
  • Page 103 12 Software description displayed and you will have the option to abort opening the listmode file or to open the listmode file and remove the compensation file from the Playlist item. Save As: The button is enabled when a single compensable Playlist item ...
  • Page 104 12 Software description Calculation group The Calculation group contains options to switch between automatic background calculation and manually triggered calculation. The Calculation group contains the following options: Background Calculation: Select the check box to turn on the automatic  background calculation. All statistics in the Results and Reports tabs of the Workspace are updated constantly in the background as the Playlist is modified.

  • Page 105: Cytometer Tab

    12 Software description 12.4.10 Cytometer tab The Cytometer tab provides controls to start cleaning procedures of the device, to sort cells if a cell sorter is connected, and to prepare compensation settings for measurements. Cleaning group The Cleaning group contains options for starting priming and cleaning procedures.
  • Page 106 12 Software description Compensation Settings group The Compensation Settings group contains options to create a fluorescence compensation setup panel in the Worklist. The options are only available if an empty Worklist item is selected but not opened in the Workspace. Click the Create Comp Panel button and select a Workspace file (CyFlow acquisition Workspace files only) to use as a source for the compensation setup panel.
  • Page 107 12 Software description Compensation Control Workspaces: The option selected here defines  the type of Workspaces that are created for the compensation setup panel. The "No negative control" option is the default selection. This will create a compensation control Workspace for each of the classified fluorescence parameters present within the original Workspace selected.
  • Page 108 12 Software description The compensation setup panel is made up of the following components and always appears in this order: Voltage adjust Workspace (always present):  Negative control sample Workspace (optional):  Compensation control Workspaces – one per fluorescence parameter ...

  • Page 109: Overlay Tab

    12 Software description Verification Workspace (optional):  When acquisition is completed on the last compensation Workspace or, if existing, on the verification sample, the calculated compensation matrix is displayed. 12.4.11 Overlay tab The Overlay tab provides controls for creating single and dual parameter plot overlays.
  • Page 110 12 Software description Overlay Wizard: Welcome Page The Welcome page introduces you to the Wizard’s functionality. Click the Next button to proceed to the Plot Selection page of the Wizard. Overlay Wizard: Plot Selection Page The Plot Selection page contains options to select the required plots to create an overlay plot.
  • Page 111 12 Software description Overlay Wizard: Overlay Layout Page The Overlay Layout Page contains options to define how the plots you have selected will be added to the overlay plot. The page contains the following options: Display all plots from all files together: Select the option to display all ...
  • Page 112 12 Software description Overlay Wizard: Overlay Labels Page This page contains options to define titles and legends to be displayed in the Overlays tab of the Workspace. The page contains the following options: Overlay Title: Enter a title for the overlay plot. ...
  • Page 113 12 Software description Quadrant Region: Click the button to preselect creating a quadrant  region on a dual parameter plot. Region Position: Select a region on a plot and click the button to modify  the position of the selected region as described for the Region Format tab (see chapter Position group on page 129).

  • Page 114: Report Tab

    12 Software description The Edit group contains the following buttons: Copy: Click the button to copy an image of the currently selected plot(s)  onto the clipboard. You can then paste them into other applications ® (Microsoft Word, PowerPoint, etc.). Delete: Click the button to delete the currently selected plots or region.
  • Page 115 12 Software description The Wizard retains settings until you open or create a new Playlist. Click the Report Wizard button to launch the Wizard. The button is disabled when there are no plots in the Workspace. The Report Wizard is made up of five types of pages: Welcome Page, ...
  • Page 116 12 Software description For each plot, the list displays the item number for the Workspace containing the plot, the X and Y parameters and the gate. To add a plot to the report, select the check box next to this plot. During a session of using the same Playlist when rerunning the Wizard, previously checked plots will be remembered.
  • Page 117 12 Software description Report Wizard: Statistics Selection page This page is only available if the "Display Statistics on Report" check box was selected on the Statistics Layout page. The Statistics Selection page enables you to select the required statistics and the order in which they are displayed on the report.
  • Page 118 12 Software description The page contains the following options: Plots per page: Use the drop-down list to specify the number of plots  per page in the report. When not reporting statistics or when statistics are aggregated at the end of the report, you can have 1, 4, 9 or 20 plots per page.
  • Page 119 12 Software description Pages group The Pages group contains options to add new pages to the report and to organise the pages of the report. The Pages group contains the following options: Add New Page: Click the button to add a new blank page to the end of ...
  • Page 120 12 Software description The option selected here will take precedence over that selected in the "Print Setup" dialogue. Size: Click the button to display a drop-down list and select the desired  page size (A4 or letter). The page size for all pages in the report is changed.
  • Page 121 12 Software description In the "Report Item" drop-down list, the page number, date and time, user name, file names and/or file paths with file names of the currently open files can be selected. In the "Primary Keyword" and "Secondary Keyword" submenus, the primary and secondary keywords from the currently open LMD file can be selected.
  • Page 122 12 Software description Line Colour: The button is enabled when one or more line objects are  selected in the report. Click the button and select the desired colour for the currently selected lines. Sort Stats: The button is enabled when a statistics box is selected in ...

  • Page 123: Views Tab

    12 Software description of the page after it has been aligned, it is automatically repositioned to fit on the page. Resize: The button allows for adjusting two or more objects to the same  height, width or size. First select the object that displays the target size, then select the objects to resize.

  • Page 124: Plot Format Tab

    12 Software description 12.4.14 Plot Format tab The Plot Format tab is only available when at least one plot is selected in the Plots area of the Workspace (see chapter 12.8.1 Plots tab of the Workspace on page 160). The Plot Format tab contains controls to adjust the settings of selected plots in the Plots area.
  • Page 125 12 Software description Gate group The Gate group contains an option to choose a gate to be applied to selected plots. Click the drop-down arrow positioned on the Gate group to display a list of all the available gates. Click the desired gate from this list to apply the gate to selected plots.
  • Page 126 12 Software description Histogram Scaling group The Histogram Scaling group contains options for scaling of the selected histograms for optimal plot visualisation. By default, the software scales the histogram plots to the maximum value on the plot. The automatic scaling ignores events in the first and last sixteen channels, unless there are no events in the remaining channels.
  • Page 127 12 Software description Histogram Smoothing Level Selection Slider: The slider is only visible  if a smoothing method (bin reduction, moving average) is selected. When Bin Reduction is selected, the slider has 4 tick marks representing levels 1 – 4. Level 1 (1024 bins) is no smoothing. When Moving Average is selected, the slider has 10 tick marks, representing levels 1 –...

  • Page 128: Region Format Tab

    12 Software description 12.4.15 Region Format tab The Region Format tab is only available when a region is selected in the Plots area. You can use the options on the Region Format tab to modify the appearance and type of a selected region. The region label can also be repositioned and regions can be tracked to other regions.
  • Page 129 12 Software description Type group The Type group contains options to change the type of a selected region. The region type of the currently selected region is highlighted. To change the region to another type (elliptical, rectangular or polygonal region), click the respective button.
  • Page 130 12 Software description Use the "Center Y" input field to position the quadrant intercept on the Y-axis. Increase the value to shift the intercept upwards. Decrease the value to shift the intercept downwards. The "Bendy X" and "Bendy Y" input fields are disabled when the "Straight"...

  • Page 131: Overlay Format Tab

    12 Software description The rules to link regions are: Only regions that are on plots that display the same parameters can be  linked. A maximum of 256 regions can be linked for each opened Workspace.  A linear region can only be linked to another linear region. ...
  • Page 132 12 Software description The Format group contains the following options: Background: Select this option to choose a background colour for all  selected plots. If overlay plots are selected, this option also affects the corresponding gallery plots. Data Color: Select this option to choose a colour for all data displayed ...
  • Page 133 12 Software description Show Plot: The button is available when one or more plots are selected  in the Galleries area. Select this option to display the selected plots in the overlay plot. Deselect this option to remove selected plots from the overlay plot.
  • Page 134 12 Software description Controls group The options in this group are available for single parameter overlay plots only. They allow one plot within a gallery to be displayed as control on all other plots within the gallery of the current overlay plot. The Controls group contains the following options: Set Control: The button is available when a single gallery plot is ...
  • Page 135 12 Software description Histogram Smoothing group The options in the Histogram Smoothing group are available when single parameter plots in a gallery or an overlay plot containing single parameter plots are selected. The options allow smoothing to be applied to histogram plots to improve the visual appearance without affecting statistical results.

  • Page 136: Panes

    12 Software description 12.5 Panes In the panes of the software window, the functions for acquiring data are provided, and informations concerning the currently open Worklist item or Playlist item are displayed. The panes that are available in the panes area can be selected and deselected in the Views tab of the Ribbon (see chapter 12.4.13 Views tab on page 123).

  • Page 137: Fcs File Information Pane

    12 Software description 12.5.1 FCS File Information pane This pane displays as a title the name of the currently active FCS file. In Worklist mode, the title is only displayed if an FCS file is present. Beneath the title, the pane displays information on the currently open FCS file.

  • Page 138: Results Hierarchy Pane

    12 Software description 12.5.2 Results Hierarchy pane This pane displays as a title the name of the currently active FCS file. In Worklist mode, the title is only displayed if an FCS file is present. Beneath the title, the pane displays the count of ungated events and count and percentage of gated events in a hierarchical view.

  • Page 139: Settings Pane

    12 Software description 12.5.3 Settings pane This pane displays a table of settings for the parameters. In Playlist mode, the table is empty and the options are disabled. In Worklist mode, the table entries can be edited for the samples to be measured. For samples already measured, the table is read only.

  • Page 140: Acquire Pane

    12 Software description value or use the up/down arrows. Threshold limits are between 1 and 2000. Threshold value can only be set when logic is not deactivated (off). Voltage: The voltage of the individual optical parameters defines the  photomultiplier signal amplification and therefore the signal intensity to be displayed.
  • Page 141 12 Software description To stop the acquisition process for that sample and save the acquired data, click the Stop button. Acquisition will move on to the next defined Worklist item to be acquired. If the current sample is the last in the acquisition sequence, acquisition will terminate.
  • Page 142 12 Software description Using the PgUp and PgDn keys of the keyboard will increase/decrease the flow rate in steps of 0.5 μl/s. Using the up/down arrow keys of the keyboard will increase/decrease the flow rate in steps of 0.1 μl/s. Scrolling the mouse scroll wheel will increase/decrease the flow rate in steps of 0.3 μl/s.

  • Page 143: Setup Pane

    12 Software description 12.5.5 Setup pane The Setup pane displays the setup options of the flow cytometer. You can select the number of events and the parameters to be displayed in the current Workspace. The options within the Setup pane are disabled when the software is in Playlist mode.

  • Page 144: Stops Pane

    12 Software description The "TVAC" check box is selected when the TVAC stop condition has  been set in the Stops pane. Using TVAC (True Volumetric Absolute Counting), a fixed volume of 200 µl between the start and stop electrodes in the sample is measured.
  • Page 145 12 Software description The pane contains the following options: Event count: Select the check box to measure a defined number of  events. In the "Stop Condition" box, click the arrow to expand a drop-down list and select the desired condition (ungated or the required gate) to base the stop event count on.

  • Page 146: Worklist Pane

    12 Software description 12.6 Worklist pane The Worklist pane is displayed in Worklist mode and by default docked to the bottom of the Workspace. A Worklist contains the predefinitions of hardware and software settings for data acquisition. Each Worklist item contains the definitions for a single sample measurement.
  • Page 147 12 Software description The Worklist buttons are: New: Click the button to create a new Worklist or a new Playlist.  Open: Click the button to open an existing Worklist or Playlist.  Save: Click the button to save the current Worklist with the same name. ...
  • Page 148 12 Software description The columns are width adjustable. To adjust the width, drag the column divider to the right of the column. If you double-click a column divider, the column will resize to display its contents optimally. The Worklist contains the following columns and associated context menus: Active: A green arrow displayed in this column indicates the currently ...
  • Page 149 12 Software description Sample ID 1 to Sample ID 12: These columns are provided for entering  sample or patient information. When data acquisition is finished, the column is disabled. Regions: This column displays the source of the region positions for ...
  • Page 150 12 Software description Stop Value: If a deviating stop condition is set in the "Stop Conditions"  column, the stop value can be entered here. LMD File Name: This column displays the LMD file name and path  created at the successful termination of acquisition. The column is automatically filled and cannot be changed.
  • Page 151 12 Software description Worklist context menu Context menus within the Worklist allow for easy access to the most commonly used functions. The context menus are disabled during acquisition. If no specific context menu is defined for the region the cursor is placed, the general context menu is displayed on right-clicking.
  • Page 152 12 Software description Panel: In this submenu, options are provided to save the panel, to insert  or replace a panel, to add an item to the panel and to clear the panel, i.e. to delete all Worklist items associated with this panel. When saving a panel, the full path and file extension are stripped and the Workspace name is used as an identifier.
  • Page 153 12 Software description Paste: The menu item is only available if the context menu is launched  from a manual entry field ("Lab #", "Sample ID" or "Stop Value" columns). Click the menu item to paste the previously copied text to the column of the selected Worklist items.

  • Page 154: Playlist Pane

    12 Software description 12.7 Playlist pane The Playlist pane is displayed in Playlist mode and by default docked to the left of the Workspace, nested with the other panes. The Playlist pane contains a Playlist. Each row of the Playlist is a Playlist item and represents one acquired sample.
  • Page 155 12 Software description Playlist toolbar The options in the Playlist toolbar allow you to navigate through a Playlist, to reorder or to delete items in the Playlist. The Playlist toolbar contains the following buttons: First File: Click the button to skip to the first file in the Playlist and open ...
  • Page 156 12 Software description The Playlist contains the following columns: First column: This column displays a sequential number or the Playlist  item. A green arrow displayed in this column indicates the active Playlist item, i.e. the Playlist item that is currently open in the Workspace. Listmode: This column displays the name of the listmode file of the ...
  • Page 157 12 Software description Selecting Playlist items To edit Playlist items, they need not to be activated but must be selected. Selected Playlist items are highlighted in blue. The following options for selecting Playlist items are available: Single click on a Playlist item to select it. ...
  • Page 158 12 Software description ® The "Use Listmode" option is enabled if the listmode file is a CyFlow file with an embedded Workspace. Selecting this option restores the embedded Workspace for the selected file. Compensation: In this submenu, options are provided to replace, save, ...
  • Page 159 12 Software description Playlist tooltips and error display When moving the cursor over the "Listmode" column of a Playlist item, a tooltip containing the corresponding LMD file path is displayed. On "Workspace" or "Compensation" columns, the tooltip contains the Workspace or compensation file path if the Playlist is not yet saved. If the Playlist is saved, the tooltip contains only the Workspace or compensation file name.

  • Page 160: Workspace

    12 Software description 12.8 Workspace In the Workspace area of the software window, the Workspace of the currently active Playlist item or Worklist item is displayed and can be edited. A Workspace includes plots, regions, gates, colour precedence, selected statistics and displayed parameters. A Workspace can be saved as a Workspace file.
  • Page 161 12 Software description Types of plots The software can display four plot types: Single Parameter Histogram Plots display the 1024 bin histogram for a  single parameter. The plot may be gated or ungated. Dual Parameter Colour Density Plots are two dimensional 256 x 256 ...
  • Page 162 12 Software description The following options are available: To add a single plot from the Previews area to the Plots area,  double-click the desired plot in the Previews area. To add several plots from the Previews area, use the + buttons in the ...
  • Page 163 12 Software description Selecting plots Selected plots are surrounded by a blue selection rectangle. The following options are available: To select a single plot within the Plots area, click on the plot.  To select or deselect multiple plots, hold Ctrl while clicking on plots. ...
  • Page 164 12 Software description The following options for editing plots in the Plots area are provided: Editing the Plot Title: To customise the title of a plot, double-click in the  top margin of the plot and enter a custom title into the edit field. The title is added to the heading of the plot.
  • Page 165 12 Software description When flipping a plot containing a quadrant region, the quadrant dividers are flipped, but the names of the quadrants are persisted. Any gate equations using these quadrant regions will be updated if required. Any linking of regions will be removed if you flip plot axes. In this case, a warning message will be displayed and you will have the option to cancel flipping the axes.
  • Page 166 12 Software description completed by clicking on the start point. If there are at least three points, the polygon is also completed by right-clicking (which will complete the polygon by adding a line from the last to first point). Double-clicking on a line inserts a new point which can then be moved to create a modified region.
  • Page 167 12 Software description control points to resize and move the rectangular region. While a rectangular region is being moved or resized, the coordinates of the top left and bottom right corners are displayed. Polygon: The polygonal region cannot be rotated and cannot be moved ...
  • Page 168 12 Software description The region name can also be changed from the Appearance group of the Region Format tab (see chapter Appearance group on page 128). The region name can be repositioned but cannot be dragged outside the plot. Region colour When a region is created (except for quadrant regions), it is automatically assigned a colour.
  • Page 169 12 Software description Plots added to the Plots area will display their scales on the X and Y axes. Fluorescence parameters default to Log scaling. Scatter or other parameters default to Lin scaling. The scaling options are: Linear Scales: Linear scaled parameters display linear tick marks and ...
  • Page 170 12 Software description The three phases of the DNA cell cycle are coloured on the cell cycle plot as follows: Red: Denotes the G0/G1 phase,  Blue: Denotes the S phase,  Green: Denotes the G2/M phase.  Additional regions cannot be applied to the cell cycle plot. The following markers are displayed on the plot: Blue Markers are debris markers allowing to set noise thresholds and to ...

  • Page 171: Results Tab Of The Workspace

    12 Software description 12.8.2 Results tab of the Workspace In the Results tab of the Workspace, a results table is displayed. Here, you can view statistics associated with the current Playlist or the current Worklist pane. You can copy statistics for use in other applications, export statistics to CSV and XML files and print statistics using the options on the File tab of the Ribbon.
  • Page 172 12 Software description analysis, denoting whether the algorithm experienced any problems, is displayed in this column. When background calculation has completed, results for all valid Playlist items will be available to be displayed in the results table. For a Worklist, the results for all items of the current panel with a listmode file are available to be displayed in the results table.
  • Page 173 12 Software description To select all rows from the currently selected one to the end of the table,  press Ctrl + Shift + End. To select all rows from the currently selected one to the beginning of the  table, press Ctrl + Shift + Home.

  • Page 174: Overlays Tab Of The Workspace

    12 Software description The information displayed in the groups can be collapsed and expanded by clicking the +/- buttons. A column header can be removed from the grouping by dragging it back to its position in the results table. When a column header is dragged back to the results table, two red arrows indicate where the header will be placed.
  • Page 175 12 Software description Creating overlay plots A new overlay plot is created by sending plots from the Plots tab of the Workspace to the Overlays tab. Select the required plot(s) in the Plots area of the Workspace. Right-click to open a context menu and select "Send selected Plot(s) to Overlay Plot".
  • Page 176 12 Software description Selecting plots Selected plots are surrounded by a blue selection rectangle. If an overlay plot is selected in the Overlays area, the associated title in the Galleries area is also highlighted. In the Galleries area, plots from only one gallery can be selected at a time. The following options are available: To select a single plot within the Overlays area or Galleries area, click on ...
  • Page 177 12 Software description Editing plot titles, legend and axis labels The following options are available: Plot title: Each overlay plot has a plot title which defaults to the overlay  number, e.g. "Overlay 1". To change a plot title, double-click on the current plot title and enter a custom plot title into the edit field.
  • Page 178 12 Software description Sending plots to the report To send plots to the report, select the desired plots in the Overlays area or the Galleries area, right-click and select the "Send selected Plot(s) to Report" option. The currently selected plots are sent to a new page at the end of the current report.

  • Page 179: Reports Tab Of The Workspace

    12 Software description of the selected plots already exist in the target overlay plot, any new plots will be added but all selected plots will be removed from the current plot. Copy selected Plot(s) to Overlay Plot takes a copy of the selected ...
  • Page 180 12 Software description The following objects can be inserted into reports: plots,  statistics boxes,  compensation matrices,  fields (objects containing derived contents such as file names, file paths,  etc.), images,  text boxes (boxes provided for entering free text), ...
  • Page 181 12 Software description context menu and select whether to send the selected plot(s) and/or their associated statistics to the report. If the last page of the report contains any objects, sent objects start on a new blank page in the report. If the last page is blank, sent objects start on this page.
  • Page 182 12 Software description Moving objects Moving the cursor over an object on a report page will change the cursor to a move icon. The object can then be dragged and dropped to new locations within the pages of a report. If objects are selected within different sections (header, footer, body) or different pages, the move cursor is not displayed and objects cannot be moved.
  • Page 183 12 Software description Resizing headers and footers To resize a header or footer, click and drag the divider of the header or footer up or down as required. The size of the header and footer is applied to all pages in the report. If the header or footer contains an object, the divider cannot be dragged beyond the border of that object.

  • Page 184: Status Bar

    12 Software description 12.9 Status bar The status bar is located at the bottom of the software window. The status bar displays the current status of calculations, of the current measurement and of compensation settings. When regions are modified, it displays the coordinates.
  • Page 185 12 Software description Status of acquisition During acquiring, the current status of the acquisition process is displayed: Part displays the absolute number of particles recorded.  Part/S displays the rate of measurement in particles per second.  µL displays the absolute volume. ...
  • Page 186 12 Software description Status of the device This symbol indicates the status of the device. Green: The device is operational.  Yellow: Maintenance is necessary.  Red: A problem occurred.  Caution symbol: The DLI is not connected.  Status of compensation settings The status of the compensation settings of the current file is displayed indicating if the file is not compensable, if the compensation is unaltered or if the compensation has been modified.

  • Page 187: Cyflow Options

    12 Software description 12.10 CyFlow Options The CyFlow Options dialogue contains controls to set various default options for the software. It is launched from the CyFlow Options button on the File tab of the Ribbon (see chapter 12.4.1 File tab on page 58). The CyFlow Options dialogue has several pages accessed by selecting the options in the list to the left of the dialogue.
  • Page 188 12 Software description Quadrant Color: The box displays the default colour for the quadrant  divider lines and is used when quadrant regions are created. This will not affect the colour of quadrant gates. The default colour is Grey-40%. To edit, click the arrow and select a colour.
  • Page 189 12 Software description The page contains the following options: Quick select region names: By default, the software offers three region  names that can be designated to regions: Lymphs, Grans, Monos. To edit, double-click on a region name. To add new region names, click the New button or double-click below the last name in the list.
  • Page 190 12 Software description V-Log Linearity (%) and V-Log Negative Section (%): These options  allow you to select the default values for scaling of V-Log parameters. The accepted range is between 0 and 100%. To edit, enter a value or use the up/down arrows.
  • Page 191 12 Software description Instrument Configuration page The Instrument Configuration page contains options to set the configuration of your device when using an offline PC to create a Worklist. The page contains the following options: ICF: The ICF (instrument capabilities file) drop-down list contains the title ...
  • Page 192 12 Software description Font pages There are six Font pages containing options to select the default font typeface and size of text displayed in the Workspace. On the right of each page, a preview plot is displayed. The following pages are available: Font - Region Labels to select the font typeface and size for region ...
  • Page 193 12 Software description Resources page The Resources page contains options to access license and software version information. The page contains the following options: License: Click the button to access the license information and update  your user license. About: Click the About button to view software information, version ...
  • Page 194 12 Software description ® CyFlow Cube 8 | Operating Manual | May 2018 | Revision 032...

  • Page 195: Index

    Index Index Compensation Matrix dialogue 91 accessories 21 Compensation tab 84 Acquire pane 140 Compensation Wizard 84 acquiring data 27 control plot 178 analysing data 37 count check beads, troubleshooting 41 Analyze tab 68 Count Check Beads (low, medium, high) 21 applications 47 Create Compensation Panel dialogue 106 assay developer 25...
  • Page 196 Index Float button 54 license information 193 flow (of sample) listmode file controls for adjusting 141 mismatch error notes 100 too low / too high 40 modifying 100 opening 158 flow cuvette 49 logging in 25 flow rate 49 logging out 26 font, default settings 192 maintenance 44 gallery view 162...
  • Page 197 Index parameter Precedence Order dialogue 70 displayed on plot 164 preparing measurements 30 optical 47 scaling 168 Preview tab 74 Parameter Properties dialogue 76 priming procedure 26 Parameters tab 76 prohibited use 9 parent gate, setting 34 particle size 47 qualification 10 personal protective equipment 12 qualification table 11...
  • Page 198 Index Ribbon 50, 58 Setup pane 143 Analyze tab 68 sheath fluid 24 Compensation tab 84 order no. 21 Cytometer tab 105 File tab 58 sheath fluid bottle 17, 19 Home tab 65 order no. 21 Overlay Format tab 131 sheath level indicator 184 Overlay tab 109 Parameters tab 76...
  • Page 199 Index user administrator 25 tab (Ribbon) user management 25, 63 Analyze 68 user roles 25 Compensation 84 Cytometer 105 File 58 Home 65 Views tab 123 Overlay 109 Overlay Format 131 Parameters 76 warnings Playlist 100 hazard levels 7 Plot Format 124 signal words 7 Preview 74 Region Format 128...

Table of Contents