SYSMEX ogt Cytocell Multiprobe CLL Instructions For Use Manual

Instructions For Use
REF: PMP 018 / PMP 017 / PMP 016 / PMP 020
Chromoprobe Multiprobe
FOR PROFESSIONAL USE ONLY
ENGLISH/FRANÇAIS/ITALIANO/DEUTSCH/ESPAÑOL
Further information available at www.cytocell.com
Fluorescence In Situ Hybridisation (FISH) is a technique that allows DNA
sequences to be detected on metaphase chromosomes or in interphase nuclei from
fixed cytogenetic samples. The technique uses DNA probes that hybridise to entire
chromosomes or single unique sequences, and serves as a powerful adjunct to
classic cytogenetics. Recent developments have meant that this valuable
technique can now be applied as an essential diagnostic tool in prenatal,
haematological and pathological chromosomal analysis. Target DNA, after fixation
and denaturation, is available for annealing to a similarly denatured, fluorescently
labelled DNA probe, which has a complementary sequence.
hybridisation, unbound and non-specifically bound DNA probe is removed and the
DNA is counterstained for visualisation. Fluorescence microscopy then allows the
visualisation of the hybridised probe on the target material.
Probe Information
Cytocell's Chromoprobe Multiprobe
detecting del (6q), Trisomy 12, del (13q), del (17p), del (11q) and rearrangements
of IGH, which lead to the 14+ markers, to be applied simultaneously to a patient
sample in a single FISH reaction. The probes have been developed to give clear
results in non-dividing cells and thus maximise the information obtained from the
technique. The device can be used for initial diagnosis, to confirm findings of routine
Cytogenetics and also for monitoring the patient over time. The device also has a
probe set for t(11;14) to enable abnormal CLL patients to be distinguished from
Mantle Cell Lymphoma patients.
Probe Specification
MYB Deletion
MYB, 6q23.3, Red (5.25-8.85ng/test)
D6Z1, 6p11.1-q11.1, Green (6.85-10.3ng/test)
The MYB probe is 183kb, labelled in red, covers the entire MYB gene and a region
telomeric to the gene, 137kb beyond the marker AFMA074ZG9. The probe mix also
contains a control probe for the 6 centromere (D6Z1) labelled in green.
®
CLL
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CLL has been developed to allow probes for
PI025/CE v009.00/2019-04-30 (H001 v3 / H002 v2 / H023 v3 / H024 v3 / H026 v2 / H027 v3 / H037 v2 / H039 v6 / H072 v3)
Chromosome 12 Enumeration
D12Z3, 12p11.1-q11.1, Red (0.28-0.47ng/test)
The Chromosome 12 Alpha Satellite Probe is a repeat sequence probe, labelled in
red, which recognises the centromeric repeat sequence D12Z3.
ATM Deletion
ATM, 11q22.3, Red (5.25-8.85ng/test)
11q12.1, Green (20.6-30.8ng/test)
The ATM probe is 182kb, labelled in red, and covers the telomeric end of the NPAT
gene and the centromeric end of the ATM gene to just beyond the D11S3347
marker. The probe mix also contains 11q12.1 control probe labelled in green,
covering a 122kb region including the SHGC-154780 marker and a 130kb region
including the SHGC-110550 marker.
IGH/BCL2 Translocation, Dual Fusion
BCL2, 18q21.33, Red (5.60-9.44ng/test)
IGH, 14q32.33, Green (13.7-20.5ng/test)
Following
The IGH/BCL2 product consists of probes, labelled in green, covering the Constant,
J, D and Variable segments of the IGH gene, and a 585kb probe, labelled in red,
covering the BCL2 and KDSR genes.
IGH Breakapart
IGHC, 14q32.33, Red (5.25-8.85ng/test)
IGHV, 14q32.33, Green (13.7-20.5ng/test)
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