Fluidigm Polaris User Manual page 30

Setting Up the Experimental Run
Set Up the Dose and Feed or Time Course Step
Set Up the Time Course Parameters for a Time Course Step
Parameter Function
Total Duration Adjust the total length of time that the Time Course
step occurs.
Exchange Interval Adjust the frequency (how often) that the dosing
reagent or feeding medium is refreshed in the cell
capture sites.
Set Dosing Automatically adjust the length of time between
Durations the staggered dosing start times of each group.
Dose Duration A (Optional, alternative to using Set Dosing
through F Durations) Manually adjust the length of time that
each group is exposed to dosing reagent.
1 Tap the Time Course image.
2 Tap anywhere in the time course image beneath Time Course:
The lower area of the screen appears that allows you to make changes to the default
parameters, as follows:
30
Minimum Value Maximum Value
2 hours
3 hours
20 minutes
20 minutes 23 hours and 40
Polaris: User Guide
24 hours
24 hours
4 hours
minutes