Fluidigm Juno User Manual
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Fluidigm Juno User Manual

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PN 100-7070 J2
Juno System
User Guide

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  • Page 1 PN 100-7070 J2 Juno System User Guide...
  • Page 2 © 2018 Fluidigm Corporation. All rights reserved. 12/2018 For technical support visit techsupport.fluidigm.com. North America +1 650 266 6100 | Toll-free (US/CAN): 866 358 4354 | techsupport@fluidigm.com Latin America +1 650 266 6100 | techsupportlatam@fluidigm.com Europe/Middle East/Africa/Russia +44 1223 598 100 | techsupporteurope@fluidigm.com Japan +81 3 3662 2150 | techsupportjapan@fluidigm.com...

  • Page 3: Table Of Contents

    Chapter 3: Using Advanced Features Safety Alerts for Instruments of Juno Safety Data Sheets Log In Log Out Chapter 1: Introducing the Juno System Manage Scripts The Juno System Enable Advanced Scripts Consumables Ordering Export Scripts to a USB Components of the Juno System...
  • Page 4 Contents Disposal of Products Appendix C: Related Documentation Appendix D: Safety General Safety Instrument Safety Symbols on the Instrument Electrical Safety Chemical Safety Juno System: User Guide...

  • Page 5: About This Guide

    For complete safety information, see Appendix Purpose This guide describes how to use the Juno™ system, including the instrument and the system software. For complete instrument specifications and unpacking/installation instructions, see the Juno System Site Requirements Guide (PN 100-7072). Safety Alerts for Chemicals...

  • Page 6: Safety Data Sheets

    Safety Data Sheets Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Fluidigm, either alone or as part of this system, go to fluidigm.com/sds search for the SDS using either the product name or the part number.

  • Page 7: Chapter 1: Introducing The Juno System

    With high-quality samples, detecting the specific targets requires a sufficient number of copies in the final preamplification mix of 4 μL. After genotyping is performed on the Juno system, the IFC is scanned on the EP1™ system or Biomark™ HD system to collect genotyping data for later analysis.

  • Page 8: Components Of The Juno System

    DVI port USB 2.0 ports Ethernet Exhaust fan port ON/OFF switch Power socket Figure 2. Back panel of the Juno instrument. There are also two USB 2.0 ports on the side of the instrument. Juno System: User Guide...
  • Page 9 Chapter 1: Introducing the Juno System Components of the Juno System White notch Figure 3. Tray and thermal chuck of the Juno instrument. The white notch is used to orient the notched corner of the IFC in the tray. Figure 4. Interface Plate Loading Fixture...

  • Page 10: Interface Plates And Ifcs

    LP 192.24 IFC System Software Ensure that you have installed the Juno system software v3.1 or later if you are using the Juno 96.96 Genotyping IFC. Install software v3.5 or later if you are using the Juno 96.96 Genotyping IFC or an IFC for the Targeted DNA Sequencing Library application. Install software v3.11.1 or later if you are using the 24.192 Dynamic Array IFC for Gene Expression.

  • Page 11: Scripts

    Chapter 1: Introducing the Juno System Scripts Scripts Basic Scripts Tab in Juno Scripts for IFCs Controller/Interface Script Descriptions Software Plate to Use* (label color) Targeted Seq One Step LP-192.24 TX (blue) One step load mix, PCR, and harvest of LP 192.24 IFC (192x)
  • Page 12 Chapter 1: Introducing the Juno System Scripts Tab in Juno Scripts for IFCs Controller/Interface Script Descriptions Software Plate to Use* (Continued) (label color) IFC Control Load 12.765 MX (black) Load the 12.765 dPCR IFC (115x) Load 48.770 MX (black) Load the 48.770 dPCR IFC (148x) Load Mix 192.24 GE...
  • Page 13 (Continued) (label color) Probe GT Juno 96.96 Fast SX (silver) One step load and PCR of Juno GT IFC (180x) for probe GT chemistry One Step 192.24 Fast RX (red) One step load and fast PCR of 192.24 GT IFC (166x) for probe GT chemistry One Step 48.48...

  • Page 14: Advanced Scripts

    Chapter 1: Introducing the Juno System Scripts Advanced Scripts NOTE To view advanced scripts on the instrument, log in as an administrator, tap Preferences, then select Enabled under Advanced Scripts. Tab in Juno Scripts for IFCs Controller/Interface Script Descriptions Software...

  • Page 15: Components Included In Shipping Box

    192.24 IFC. The adapter fits onto the Barrier Tape Applicator to apply barrier tape to the LP 48.48 IFC. Optional Components Depending on the type of IFC used with Juno, you will need additional interface plates. Component Purpose SX Interface Plate (PN 100- 6368) Supports the Juno 96.96 Genotyping IFC and has a...

  • Page 16: System Functions

    The system uses a thermal stack to provide rapid, accurate, uniform heating and cooling. Air Options The Juno system has an internal compressor to generate compressed air and draws in ambient air by default. To use in-house compressed air, attach 1/4-inch tubing into the air inlet on the back of the system.

  • Page 17: Conformity Symbols On The Instrument

    Indicates conformity with safety requirements for Canada and the United States. Indicates conformity with European Union requirements for safety and electromagnetic compatibility. Refer to the Juno System Site Requirements Guide (PN 100-7072) for more detailed information on the recommended environmental conditions. Juno System: User Guide...

  • Page 18: Chapter 2: Getting Started With Juno

    Chapter 2: Getting Started with Juno To run the basic features of Juno, use the instrument as a guest. To use advanced features, log in as an administrator. (See Using Advanced Features of Juno on page 36.) NOTE Ensure that you have installed the Juno system software v3.1 or later if you are using the Juno 96.96 Genotyping IFC.
  • Page 19 Chapter 2: Getting Started with Juno Start the Juno System The startup screen displays after 10–15 seconds: Next, the Start screen displays: The instrument initializes all system components, including communication protocol, vacuum and temperature sensors, software parameters, instrument configuration, and calibration data.

  • Page 20: How To Use The Juno Touchscreen

    How to Use the Juno Touchscreen Start a Run Load an IFC See the appropriate Fluidigm documentation to prepare an IFC for loading the instrument. For related documentation, go to fluidigm.com/documents Install the appropriate interface plate according to Table 1 page 10.
  • Page 21 Chapter 2: Getting Started with Juno Start a Run NOTE If you have not yet installed an interface plate, the screen displays “Interface plate is required.” (See the following figure.) If you tap OPEN without installing an interface plate, the No Interface Plate is Installed screen displays. Tap Install Interface Plate.
  • Page 22 Chapter 2: Getting Started with Juno Start a Run Tap LOAD: The screen of available protocols or scripts displays, and the IFC barcode is on the bottom right of the screen: IMPORTANT • If a barcode error message displays, check the IFC position and direction, then reload the IFC.

  • Page 23: Select A Script

    Chapter 2: Getting Started with Juno Start a Run Select a Script Install the appropriate interface plate according to Table 1 page 10. (See Switch an Interface Plate on page 26.) After the IFC loads into the instrument, the system displays the scripts available for the installed interface plate and loaded IFC.

  • Page 24: Schedule The Harvest With The Lp 192.24 Ifc Or Lp 48.48 Ifc

    Chapter 2: Getting Started with Juno Start a Run Schedule the Harvest with the LP 192.24 IFC or LP 48.48 IFC Select the appropriate script to run the LP 192.24 IFC or LP 48.48 IFC. Slide the vertical marker along the bar to set the harvest time. For example:...
  • Page 25 Chapter 2: Getting Started with Juno Start a Run A status bar at the bottom of the screen displays an estimated time to completion. To abort the run, tap ABORT: At the end of the run, tap EJECT to remove the IFC: Tap CLOSE at the bottom right of the screen to close the tray.

  • Page 26: Switch An Interface Plate

    Start a Run Switch an Interface Plate You may need to switch interface plates when you change applications or IFCs on the Juno system. If there is no interface plate installed on the instrument, install the correct one. (See Install an Interface Plate on page 29 for more information.)
  • Page 27 Chapter 2: Getting Started with Juno Start a Run Review the guidelines on the screen to select the correct interface plate. (Also see Interface Plates and IFCs on page 10 Components of the Juno System on page Juno System: User Guide...
  • Page 28 Chapter 2: Getting Started with Juno Start a Run Remove the interface plate to be installed from its storage container and ensure that it is clean. (See Clean an Interface Plate on page 51.) • Swivel-style storage container: Pull the tray at the notch to swing out the tray with the correct interface plate.

  • Page 29: Install An Interface Plate

    Chapter 2: Getting Started with Juno Start a Run Install an Interface Plate Every IFC requires an interface plate. If there is no interface plate installed or you have tapped OPEN before installing an interface plate, follow this procedure to install one. If the...
  • Page 30 Chapter 2: Getting Started with Juno Start a Run Remove the Interface Plate Loading Fixture from its storage container. Ensure that there is no IFC on the tray, then place the loading fixture on it in the direction of the arrows with THIS SIDE UP facing up. Barcode numbers on the fixture label face...
  • Page 31 Review the guidelines on the screen to select the correct interface plate (also see Interface Plates and IFCs on page 10 Components of the Juno System on page Remove the interface plate to be installed from its storage container and ensure that it is clean.

  • Page 32: View System Information

    Chapter 2: Getting Started with Juno View System Information Seat the interface plate securely on top of the loading fixture (the MX Interface Plate is an example). Insert the interface plate in the direction of the arrows with THIS SIDE UP facing up: On the Interface Plate screen, tap INSTALL.

  • Page 33: Export A Log

    Chapter 2: Getting Started with Juno Export a Log Export a Log Plug a USB drive or equivalent storage device into a USB port of the instrument. Wait a few seconds for the instrument to recognize the USB drive. If the instrument cannot find the USB drive, the screen displays: On the Start screen, tap TOOLS.

  • Page 34: Shut Down The System And Then The Instrument

    Chapter 2: Getting Started with Juno Shut Down the System and Then the Instrument Unplug the USB drive from the instrument. Shut Down the System and Then the Instrument Perform the system shutdown procedure to safely shut down the computer system before turning OFF the instrument power.
  • Page 35 Chapter 2: Getting Started with Juno Shut Down the System and Then the Instrument Verify system and instrument shutdown by noting the power and standby indicator: Instrument State Screen Power and Standby Indicator Normal operation Sleep mode Blinking After system shut down...

  • Page 36: Chapter 3: Using Advanced Features Of Juno

    Juno To use advanced operations on Juno™, such as managing users and scripts and updating the system, log in as an administrator. To run basic features, use Juno as a guest. (See Chapter 2: Getting Started with Juno on page 18.)

  • Page 37: Manage Scripts

    Chapter 3: Using Advanced Features of Juno Manage Scripts Manage Scripts With the Manage Scripts tool, you can: • Enable Advanced Scripts • Export Scripts to a USB • Install Scripts from a USB on page 38 Enable Advanced Scripts Ensure that you have logged in to the system as administrator.

  • Page 38: Install Scripts From A Usb

    Chapter 3: Using Advanced Features of Juno Manage Scripts Tap the script name to export (copy) the script from the instrument to a Scripts folder on the USB. A screen confirms copying was successful. (Optional) Tap EXPORT ALL to export all scripts to the USB. A status screen displays to confirm the software/firmware was updated successfully.

  • Page 39: Modify Scripts

    Chapter 3: Using Advanced Features of Juno Modify Scripts Modify Scripts IMPORTANT You can change the properties of added and saved scripts. You cannot modify system scripts. You can only import modified scripts. To View the Scripts to Modify Ensure that you have logged in to the system as administrator. (See Log In on page 36.)

  • Page 40: To Change General Properties Of A Script

    Chapter 3: Using Advanced Features of Juno Modify Scripts Tap the script name to modify the general or thermal properties of the script: To Change General Properties of a Script On the Modify screen, tap the saved script to modify. (See...
  • Page 41 Chapter 3: Using Advanced Features of Juno Modify Scripts Tap a PCR protocol, such as Preamplification Step or PCR Step: Change the properties as follows: Property Steps to Change Segment name and repeats (for Tap and hold the segment name.

  • Page 42: To Remove A Script

    Chapter 3: Using Advanced Features of Juno Manage Users To Remove a Script Ensure that you have logged in to the system as administrator. (See Log In on page 36.) On the Start screen, tap TOOLS, then tap Manage Scripts to display the Manage Scripts screen.
  • Page 43 Chapter 3: Using Advanced Features of Juno Manage Users Tap ADMIN OR STANDARD, and then tap the edit button: A keyboard displays. Enter the user name. Tap OK: Next to Password, tap the edit button. After the keypad displays, enter the alphanumeric user password, tap OK, and then SAVE.

  • Page 44: Change The Date And Time

    Chapter 3: Using Advanced Features of Juno Change the Date and Time Change the Date and Time Ensure that you have logged in to the system as administrator. (See Log In on page 36.) On the Tools screen, tap Preferences. On the Preferences screen, the current date and time display.

  • Page 45: Update The System

    Chapter 3: Using Advanced Features of Juno Update the System Update the System Copy the appropriate update package (*.pak) to a USB. Plug the USB into a USB port of the instrument. (Optional) On the Start screen, tap TOOLS to note the current version of the system software.

  • Page 46: Enable In-House Air

    Ensure that you have logged in to the system as administrator. (See Log In on page 36.) Connect 1/4-inch outside diameter tubing to the air inlet at the back of the Juno system: In-house air inlet From the system software, tap Tools.

  • Page 47: Appendix A: Troubleshooting

    1 hour. No display after power is Juno system power Turn off the Juno system power switch on the back panel. turned on and fan is not supply not fully reset Wait 10 seconds and reboot by turning on the power switch.

  • Page 48: Loading

    Interface plate is not • Switch or install the interface plate with an interface “The carrier is not correct for the IFC plate supported on the Juno system and that supports detected.” the IFC. (See Components of the Juno System on •...

  • Page 49: Running

    Unable to start the Clean • Cleaning Plate Eject the interface plate and put the Cleaning Plate into the System run missing tray. (See Clean the Juno System on page 53.) • Interface plate not removed Juno System: User Guide...

  • Page 50: Appendix B: Preventive Maintenance, Decontamination, And Disposal

    Fluidigm, verify with Fluidigm technical support that the proposed method will not damage the equipment This section describes how to clean and maintain your Juno system for optimal performance. Preventive Maintenance Recommendations For optimal performance of your Juno system, we recommend: •...

  • Page 51: Clean An Interface Plate

    • Before you store an interface plate in the interface plate storage container. On the Start screen, tap TOOLS. On the Tools screen, tap Interface Plate to display the Interface Plate screen. Tap CLEAN INTERFACE PLATE: Juno System: User Guide...
  • Page 52 THIS SIDE UP facing up. Barcode numbers on the fixture label face out: Seat the cleaned interface plate on top of the fixture. (The MX Interface Plate is an example.) Insert the interface plate in the direction of the arrows with THIS SIDE UP facing up: Juno System: User Guide...

  • Page 53: Clean The Juno System

    Remove the fixture and store it in the interface plate storage container. Tap CLOSE, and then tap BACK twice to return to the Start screen. Clean the Juno System Clean the instrument after every 250 runs. After 250 runs, the instrument will not operate until the instrument is cleaned.
  • Page 54 THIS SIDE UP facing up. Barcode numbers on the fixture label face out. Seat the desired and cleaned interface plate securely on top of the fixture. Insert the interface plate in the direction of the arrows with THIS SIDE UP facing up. Tap INSTALL: Juno System: User Guide...

  • Page 55: Clean The Touchscreen

    • Do not use bleach to clean the instrument as it is corrosive to metal. • Before using a cleaning or decontamination method other than those recommended by Fluidigm, verify with Fluidigm technical support that the proposed method will not damage the equipment.

  • Page 56: Decontamination And Disposal

    For more information, see the BMBL guidelines online at cdc.gov/biosafety/publications/index.htm. Ensure that the Juno system is cleaned and/or decontaminated prior to servicing the equipment, removing it from use, or transporting it for disposal. Refer to the instructions contained in this document and use only those materials specified.

  • Page 57: Disposal Of Products

    Do not dispose of this product in unsorted municipal waste. This equipment may contain hazardous substances that could affect health and the environment. Use appropriate take- back systems when disposing of materials and equipment. Learn more at fluidigm.com/compliance. Juno System: User Guide...

  • Page 58: Appendix C: Related Documentation

    Appendix C: Related Documentation The latest Juno system usage and application-specific documentation is available for download at fluidigm.com/documents. Document Title Part Number Juno System Site Requirements Guide 100-7072 Juno Usage Quick Reference 100-7713 Control Line Fluid Loading Procedure Quick Reference...

  • Page 59: Appendix D: Safety

    For translations of the instrument safety information, see Safety Information for Genomics Instruments (PN 101-6810). General Safety In addition to your site-specific safety requirements, Fluidigm recommends the following general safety guidelines in all laboratory and manufacturing areas: • Use the appropriate personal protective equipment (PPE): safety glasses, fully enclosed shoes, lab coats, and gloves, according to your laboratory safety practices.

  • Page 60: Symbols On The Instrument

    The main power disconnect is on the rear panel of the instrument. WARNING ELECTRICAL HAZARD. DO NOT REMOVE THE COVERS. Electrical shock can result if the instrument is operated without its protective covers. No internal components are serviceable by the user. Juno System: User Guide...

  • Page 61: Chemical Safety

    When working with any chemicals, refer to the applicable safety data sheets (SDSs) provided by the manufacturer or supplier. Juno System: User Guide...
  • Page 62 Back Cover 7000 Shoreline Court, Suite 100 South San Francisco, CA 94080 USA T: 650 266 6000 For technical support visit techsupport.fluidigm.com...

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