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Fluidigm Callisto User Manual

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PN 100-7598 D1
Callisto System
USER GUIDE

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  • Page 1 PN 100-7598 D1 Callisto System USER GUIDE...
  • Page 2 © 2017 Fluidigm Corporation. All rights reserved. 10/2017 For technical support visit fluidigm.com/support. North America +1 650 266 6100 | Toll-free: 866 358 4354 in the US | techsupport@fluidigm.com Europe +33 1 60 92 42 40 | techsupporteurope@fluidigm.com China (excluding Hong Kong) +86 21 3255 8368 | techsupportchina@fluidigm.com Japan +81 3 3662 2150 | techsupportjapan@fluidigm.com...

  • Page 3: Table Of Contents

    Appendix A: Troubleshooting How to Use the Callisto Touchscreen Observation and Possible Course of Action Run an Experiment Powering On and Off Load the Callisto Adherent Cell Culture IFC-EC Loading Interface Plate Assembly in the Instrument Running Select and Run an Experiment...
  • Page 4 Cleaning the Cell Culture EC Interface Plate Replacing Filters in the EC Interface Plate Cleaning the Touchscreen Preventive Maintenance Disposal of IFCs Fuse Replacement Decontamination of the Callisto System Biological Agents Hazardous Chemicals Radioactive Materials Appendix C: Related Documentation Appendix D: Safety...

  • Page 5: About This Guide

    In addition, ensure that you have installed or upgraded to Callisto™ Experiment Planner v2.0 or later on your computer and Callisto system software v3.8 or later on the instrument. [See...

  • Page 6: Safety Alerts For Instruments

    Safety Data Sheets Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Fluidigm, either alone or as part of this system, go to fluidigm.com/sds and search for the SDS using either the product name or the part number.

  • Page 7: Chapter 1: Introducing The Callisto System

    IFC (Culture Integrated Fluidic Circuit). Specifically, these conditions are regulated by an environmental control located above the IFC in the instrument. Callisto is versatile. The IFC has two cell inputs, 16 reagent inputs, three common medium inputs, and 32 addressable culture chambers to maintain 20–3,000 adherent cells per cell culture chamber.

  • Page 8: Components Of The Callisto System

    Digital visual interface (DVI) port USB 2.0 ports Ethernet Exhaust fan port On/off switch Power socket Figure 2. Back panel of the Callisto instrument. There are also two USB 2.0 ports on the side of the instrument. Callisto System: User Guide...
  • Page 9 Thermal chuck White notch Figure 3. Tray and thermal chuck of the Callisto instrument. The white notch is used to orient the notched corner of the IFC in the tray. The thermal chuck controls the temperature of the cells by heating and cooling the IFC.
  • Page 10 Figure 4. Exploded view of the Callisto Adherent Cell Culture IFC-environmental control (EC) interface plate assembly on the Callisto shuttle. The EC interface plate lies on top of the IFC. Barcode numbers on the IFC label face out. The environmental chamber is the space between the EC interface plate and the center of the IFC.

  • Page 11: System Software

    Harvest Hydration & Control Line Medium Figure 5. Callisto IFC loading map for the Callisto Adherent Cell Culture IFC (PN 101-0008) System Software Before using this guide, ensure that you have installed or upgraded to Callisto Experiment Planner v2.0 or later on your computer and Callisto system software v3.8 or later on the instrument.

  • Page 12: Components Included In Shipping Box

    Replacement filters for the Callisto Adherent (PN 101-0004) Cell Culture IFC. NOTE To order additional items, contact Fluidigm sales. For phone or email contact information, go to fluidigm.com. User Documentation The latest Callisto documentation is available for download at fluidigm.com/documents.
  • Page 13 Indicates conformity with safety requirements for Canada and the United States. Indicates conformity with European Union requirements for safety and electromagnetic compatibility. Refer to Callisto Site Requirements Guide (PN 100-7601) for more detailed information on the recommended environmental conditions. Callisto System: User Guide...

  • Page 14: Pre-Mixed Gas Use

    The allowable pressure input is listed on the back of the Callisto system. For more information on the allowable pressure input, contact Fluidigm technical support.

  • Page 15: Chapter 2: Getting Started With Callisto

    Callisto system by a Fluidigm service representative. If you choose to lift or move the Callisto system after it has been installed, do not attempt to do so without the assistance of others. Use appropriate moving equipment and proper lifting techniques to minimize the chance of physical injury.
  • Page 16 Chapter 2: Getting Started with Callisto Start the Callisto System On/off switch NOTE Switching the back power button on or off turns on or off all components of the system. In contrast, pushing the front power button for ~2 seconds wakes up the instrument if it is in sleep mode and prompts the system to display the system shutdown menu.
  • Page 17 Chapter 2: Getting Started with Callisto Start the Callisto System The startup screen displays after 10–15 seconds: Next, the Start screen displays: The instrument initializes all system components, including communication protocol, vacuum and temperature sensors, software parameters, instrument configuration, calibration data, and the environmental controller.

  • Page 18: How To Use The Callisto Touchscreen

    Chapter 2: Getting Started with Callisto How to Use the Callisto Touchscreen How to Use the Callisto Touchscreen Lime green: action Figure 6. The Start screen on Callisto. The screen displays normal status in white and typical actions in light green. Callisto System: User Guide...
  • Page 19 How to Use the Callisto Touchscreen Orange: alert Orange: notification Figure 7. An error screen (top) on Callisto displaying an alert in orange. A notification in orange (bottom) by Next Attendance. Alerts and notifications also display on the Instrument Viewer in Callisto Experiment Planner.
  • Page 20 Chapter 2: Getting Started with Callisto How to Use the Callisto Touchscreen Figure 8. An example of an Experiment screen that displays while an experiment is running on Callisto Location Information Gas flow through the IFC-EC interface plate assembly in /min.

  • Page 21: Run An Experiment

    USB port of Callisto. [See the Callisto Experiment Planner User Guide (PN 100-8806).] Prepare the Adherent Cell Culture IFC-EC interface plate assembly (IFC-EC interface plate assembly) for loading into the instrument (See the Callisto System Getting Started Guide). Tap OPEN.
  • Page 22 Tap LOAD: The screen of available experiments displays, including published experiments from Callisto Experiment Planner. If you plugged a USB drive into the instrument, available experiments to run are displayed. For example: CAUTION PINCH HAZARD. The instrument door and tray can pinch your hand.

  • Page 23: Select And Run An Experiment

    IFC. If the instrument still fails to load the IFC, the instrument displays the Enter IFC barcode screen. Enter the barcode manually, and then tap LOAD. • If the Callisto Adherent Cell Culture IFC is incorrect or in the wrong orientation, a Load error screen displays: Select and Run an Experiment The screen captures are examples.
  • Page 24 Verify that the displayed steps of the experiment on the Steps screen match the experiment plan or modified execution file that was created in Callisto Experiment Planner. Tap the bars or swipe the screen to scroll through the steps. The highlighted bar is the step currently running: Ensure that gas flow to the instrument is on and the tank is ≥1/4 full.
  • Page 25 Chapter 2: Getting Started with Callisto Run an Experiment From the experiment screen, confirm that you have added reagents and media according to the appropriate Cell Culture Worksheet. The first pipetting map from the Cell Culture Worksheets, including the reagents to pipet, displays on the screen: Tap CONFIRM.
  • Page 26 RESUME. For detailed information on steps, see the Callisto System Getting Started Guide (PN 100-7599). (Optional) When this function is enabled, tap PAUSE to perform a task off of the instrument such as imaging the Callisto Adherent Cell Culture IFC or harvesting materials from the IFC.
  • Page 27 Chapter 2: Getting Started with Callisto Run an Experiment At the end of the experiment, tap EJECT to remove the IFC-EC interface plate assembly: Tap CLOSE at the bottom right of the screen to close the tray. Callisto System: User Guide...

  • Page 28: Perform Step Operations

    Chapter 2: Getting Started with Callisto Perform Step Operations Perform Step Operations All Steps Time of If You Want to... Then... Operation Before or after Skip a step. 1 Tap the new step. a step is 2 Tap YES to confirm that you want to skip the current step.

  • Page 29: Modify An Experiment

    Modify an Experiment You can modify an experiment before, during, or after a run on Callisto. For example, while an experiment is running, export the experiment [the experiment (.iee) file], modify it in Experiment Planner, and then import the modified experiment to Callisto while it is still running the experiment.
  • Page 30 IMPORTANT You cannot skip Pre-treatment part A, Pre-treatment part B, or Cell loading steps. These steps are required to proceed with the experiment on Callisto. If desired, stop a step or wait between steps to skip a step. Tap the previous or proceeding step from the current step (shaded) to skip a step.

  • Page 31: Modify The Duration Of A Feeding Or Dosing Step On Callisto

    Chapter 2: Getting Started with Callisto Modify an Experiment Modify the Duration of a Feeding or Dosing Step on Callisto On the Steps screen, while a step is running, tap MODIFY. Adjust the duration of the step by moving the slider, and then click OK. The...

  • Page 32: Modify An Experiment Remotely

    Set a Personal Identification Number (PIN) to Access the Instrument Remotely on page Modify an Experiment with a USB Drive You can modify an experiment during or after a run on Callisto. During a run, you can only modify uncompleted steps. IMPORTANT...
  • Page 33 Plug the USB drive with the modified execution (.iee) file into a USB port on Callisto. To import the experiment file to the instrument: •...

  • Page 34: Import And Export Experiment Plans And Execution Files

    Import and Export Experiment Plans and Execution Files Import and Export Experiment Plans and Execution Files Plug a USB drive into a USB port of Callisto. On the Start screen, tap TOOLS. On the Tools screen, tap Manage Experiments. The Manage Experiments screen...

  • Page 35: View System Information

    Chapter 2: Getting Started with Callisto View System Information NOTE You can also import and export experiment files to a USB drive by tapping MANAGE during an experiment and then tapping Export Experiment or Import Experiment. You do not need to log in as an administrator.

  • Page 36: View Experiment Runs

    Set a Personal Identification Number (PIN) to Access the Instrument Remotely A PIN restricts access to Callisto from Callisto Experiment Planner. Remote access from Callisto Experiment Planner allows you to view instrument status from the software instrument viewer and open experiment plan (.iep) and execution (.iee) files.

  • Page 37: Sort And Show Experiments

    Chapter 2: Getting Started with Callisto Sort and Show Experiments Sort and Show Experiments On the Load screen, tap TOOLS. On the Tools screen, tap Preferences. On the Preferences screen, you can tap the radio button to sort experiments (.iep and .iee files) alphabetically or chronologically or tap a radio button to show all...
  • Page 38 • If you must power down the system due to an unrecoverable error, record the error code and tap SHUTDOWN. The computer shuts down (the screen goes dark). Report the error code to Fluidigm technical support. Wait 10 seconds (the screen goes dark). The computer shuts down only.
  • Page 39 Chapter 2: Getting Started with Callisto Shut Down the System and the Instrument To restart the system, toggle the power switch to on. (See Start the Callisto System on page 15.) Callisto System: User Guide...

  • Page 40: Chapter 3: Customizing Callisto

    Chapter 3: Customizing Callisto Callisto requires that you log in as an administrator in order to manage users and files, update the system, and change date and time. Log In Tap LOG IN at lower left of the Start screen. The Log In screen displays.

  • Page 41: Manage Users

    Chapter 3: Customizing Callisto Manage Users Manage Users Add or edit users by changing settings under Manage Users. Ensure that you have logged in to the system as administrator. (See Log In on page 40.) On the Load screen, tap TOOLS. The TOOLS button displays only when an experiment is not running.
  • Page 42 Chapter 3: Customizing Callisto Manage Users Tap STANDARD or ADMIN, and then tap the edit button: A keyboard displays. Enter the user name and tap OK: Next to Password, tap the edit button. After the keypad displays, enter the user password, tap OK, and then SAVE.
  • Page 43 Callisto Adherent Cell Culture IFC needs attention (for example, feeding) or if there is an error with the EC interface plate. To use email notification, ensure that the Callisto instrument is on a network and remains connected to the network during an experiment run. The network connector is on the back of the instrument.

  • Page 44: Change The Date And Time

    Chapter 3: Customizing Callisto Change the Date and Time (Optional) Edit existing user accounts by tapping the user name. For example, to remove a user, tap the user name and tap REMOVE. Change the Date and Time Ensure that you have logged in to the system as administrator. (See...
  • Page 45 Chapter 3: Customizing Callisto Change the Date and Time Tap CHANGE TIME ZONE to set the correct time zone for the location of the instrument: Tap OK. Callisto System: User Guide...

  • Page 46: Update The System

    Chapter 3: Customizing Callisto Update the System Select a date format, and slide a value up or down to adjust the date and time. Tap Update the System Copy the appropriate update package (.pak) to a USB drive. Plug the USB drive into a USB port of the instrument.

  • Page 47: Enable Remote Access

    Enable and disable remote access to Callisto from Callisto Experiment Planner. If Enable Remote Access is unchecked (disabled), in Callisto Experiment Planner, you will not be able enter a personal identification number (PIN) to access Callisto, view instrument status on the instrument viewer, or open files from the instrument.

  • Page 48: (Optional) Enable Compressed Air

    Ensure that you have logged in to the system as administrator. (See Log In on page 40). Connect 1/4 inch outside diameter tubing to the air inlet at the back of the Callisto system: Compressed air input From the system software, tap Tools.
  • Page 49 Chapter 3: Customizing Callisto (Optional) Enable Compressed Air For compressed air input, under Alternate Air Input, tap Enabled: Callisto System: User Guide...

  • Page 50: Appendix A: Troubleshooting

    Appendix A: Troubleshooting For troubleshooting the Callisto Adherent Cell Culture IFC, preventive maintenance, and instructions, see the Callisto System Getting Started User Guide (PN 100-7599). For troubleshooting the Callisto Experiment Planner, see the Callisto Experiment Planner User Guide (PN 100-8806).

  • Page 51: Loading

    • Enter barcode manually in the Enter IFC barcode read barcode on IFC screen. • Message: “Unable to • EC interface plate or • Use the EC interface plate with the Callisto Adherent load.” IFC missing Cell Culture IFC. (See Components of the Callisto System on page 8.) If necessary, tap OPEN to remove...

  • Page 52: Running

    • Reinsert the USB drive in the instrument. recognized • Insert the USB drive into a different port. Experiment plan (.iep Return to Callisto Experiment Planner and validate the file) or execution (.iee) experiment plan or execution file. Select the validated plan file not validated on Callisto.

  • Page 53: Environmental Control Warnings

    IFC and the EC interface plate. If there are irregularities in the environmental control, Callisto warns you. To allow time for Callisto to verify a warning, the instrument emails notification of an environmental control warning 10 minutes after the instrument first detects a potential problem.

  • Page 54: Appendix B: Maintenance, Decontamination, And Disposal

    Appendix B: Maintenance, Decontamination, and Disposal Cleaning and Maintenance This section describes how to clean and maintain your Callisto system for optimal performance. Before using a cleaning or decontamination method other than those recommended by Fluidigm, verify with Fluidigm technical support that the proposed method will not harm the instrument.

  • Page 55: Cleaning The Cell Culture Ec Interface Plate

    EC interface plate from the IFC and clean the EC interface plate in a cell culture hood. • Before you store an EC interface plate in the Interface Plate Storage Kit. Use the cleaning swabs and lint-free cleaning cloths supplied in the Callisto Accessories Kit (PN 101-0003). Callisto System: User Guide...
  • Page 56 EC interface plate, where the sensor is attached and pointing up, to clean IMPORTANT Do not touch the sensor. Sensor Spray a cleaning swab with 70% ethanol or 70% isopropyl alcohol: IMPORTANT Do not spray alcohol directly on the EC interface plate. Callisto System: User Guide...

  • Page 57: Replacing Filters In The Ec Interface Plate

    • The EC interface plate is suspected of being contaminated. Replace the filters in the contaminated area. • The Callisto Adherent Cell Culture IFC is contaminated. Replace all filters in the EC interface plate. • A filter is wet. A wet filter appears darker than dry filters.
  • Page 58 Appropriately discard the used filter. With a gloved hand, insert a new filter into the empty hole on top of the EC interface plate. Both sides of the filter are identical. Callisto System: User Guide...

  • Page 59: Cleaning The Touchscreen

    • Do not use bleach to clean the instrument as it is corrosive to metal. • Before using a cleaning or decontamination method other than those recommended by Fluidigm, verify with Fluidigm technical support that the proposed method will not damage the equipment.
  • Page 60 Replace both fuses (8 A, 250 V type, time-lag T fuse), and reinstall the fuse holder. Follow the arrow marks in the fuse holder to match the casing. Plug the instrument into the wall outlet, and toggle the main power switch ON. Callisto System: User Guide...

  • Page 61: Decontamination Of The Callisto System

    Decontamination of the Callisto System Decontamination of the Callisto System Ensure that the Callisto system is cleaned and/or decontaminated prior to servicing the equipment, removing it from use, or transporting it for disposal. Refer to the instructions contained in this document and use only those materials specified.

  • Page 62: Appendix C: Related Documentation

    Callisto System Usage (Quick Reference) 100-7715 Callisto Experiment Planner User Guide 100-8806 Callisto Site Installation Guide 100-7597 Run the Callisto System Test 001-A Protocol 101-0681 Run the Standard Pressure Test (SPT) with the Universal Pressure Test Fixture (uPTF) Protocol 101-0759 Callisto System: User Guide...

  • Page 63: Appendix D: Safety

    Information for Genomics Instruments (PN 101-6810). General Safety In addition to your site-specific safety requirements, Fluidigm recommends the following general safety guidelines in all laboratory and manufacturing areas: • Use personal protective equipment (PPE): safety glasses, fully enclosed shoes, lab coats, and gloves, according to your laboratory safety practices.

  • Page 64: Symbols On The Instrument

    Indicates specific chemical harm. Indicates hazardous, toxic, or very toxic materials that are very hazardous to health or potentially fatal when inhaled, swallowed, or in contact with the skin. Indicates a health hazard. Power and standby symbol. Callisto System: User Guide...
  • Page 65 To minimize negative environmental impact from disposal of electronic waste, do not dispose of electronic waste in unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provision. Contact customer service for information about responsible disposal options. Callisto System: User Guide...

  • Page 66: Electrical Safety

    When working with any chemicals, refer to the applicable safety data sheets (SDSs) provided by the manufacturer or supplier. Callisto System: User Guide...
  • Page 67 For technical support visit fluidigm.com/support.

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