Set Up The Overview; Set Up The Prime Step - Fluidigm Polaris User Manual

Set Up the Overview

Beneath the Overview area, you can tap the large gray box and enter a description
of the experiment to be run. A description can be added or changed at any time
during the run.

Set Up the Prime Step

The Prime step allows you to specify whether the cells will be maintained in
suspension (default) or adherent conditions.
The Adherent Cell Priming option requires the use of an additional inlet for loading
the extracellular matrix (ECM) of your choice, which coats the capture sites and
permits adherent cell attachment. Suspension cell priming does not include an extra-
cellular matrix coating step.
1 To change the experiment details for the Prime step to adherent cells, tap
Suspension Cell Priming beneath Prime:
2 The lower area of the screen appears that allows you to make the change. Tap
Suspension Cell Priming in the Priming Type box to change the value to Adherent
Cell Priming:
This action also changes the display of the Prime step to Adherent Cell Priming:
Polaris: User Guide
Setting Up the Experimental Run
Set Up the Overview
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