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Translation of the original operating manual 08/2012 Rev. 1.21 of 08/05/2020 Sigma 3-18KHS; ; part no. 0701202 Sigma 3-18KHS from serial no. 147220 Refrigerated Centrifuge with Heating System Operating Manual Please retain for later use!
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 2 / 89 Translation of the original operating manual, part no. 0701202...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 4 / 89 Translation of the original operating manual, part no. 0701202...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Table of contents Storage and transport ....................... 24 Dimensions and weight ....................24 Storage conditions ......................24 Notes on transport ......................24 Packaging ........................25 Transport safety device ....................25 Set-up and connection ...................... 26 Installation site ........................
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Table of contents Malfunctions and error correction ..................56 General malfunctions ...................... 56 7.1.1 Emergency lid release ....................57 Table of error codes ......................58 Service contact ....................... 59 Maintenance and service ....................60 Maintenance ........................
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Table of contents Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 8 / 89 Translation of the original operating manual, part no. 0701202...
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 34 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0020 Symbol- und Hi nweis erklär ung en @ 26\mod_1405319013955_68.docx @ 191947 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 3 Safety Pos : 36 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0030 Ver antw ortung des Betrei bers @ 26\mod_1405319015364_68.docx @ 191961 @ 2 @ 1 → Responsibility of the operator The operator is responsible for authorising only qualified personnel to work on the centrifuge (see chapter 3.4 - "Operating personnel").
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 45 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0020 M ec hanisc he Sic her heit (auß er 1-16/1-16K/1-7) @ 26\mod_1405319024314_68.docx @ 192059 @ 3 @ 1 →...
Leerz eile @ 0\m od_1202116244500_0.doc x @ 114 @ @ 1 Pos : 49 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0040 C hemische und bi ologisc he Sic her hei t @ 26\m od_1405319028141_68.docx @ 192101 @ 3 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 3 Safety Pos : 51 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0060-0050 Sicherheits hi nweis e z ur Z entrifug ation @ 26\mod_1405319029632_68.doc x @ 192115 @ 3 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 3 Safety Pos : 73 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/030 Sic her heit/030-0080 Ver halten bei Gefahren und Unfäll en @ 27\mod_1405319051188_68.docx @ 192325 @ 2 @ 1 → Measures in the event of hazards and accidents •...
→ Power supply Pos : 95 /100 Sigma/100 BA Zentrifugen Sigma (Standar dmodule)/050 Aufstellung und Ans chl uss/050- 0020-0011 Ansc hl uss art (Kaltger ätesteck er+Sc hutzsc halter) @ 32\mod_1417600691402_68.docx @ 222498 @ 3 @ 1 5.2.1 Type of connection...
Pos : 107 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/060- 0020-0019 Ei ns etz en v on Rotor en und Zubehör ( auß er 8K)-------------------------------- @ 27\m od_1405319204492_68.docx...
Pos : 114 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/060- 0020-0020-0030 Eins etzen v on Z ubehör 4plätzig 2-16P, 2-16KL, 3- 16L, 3- 16KL, 3-18KS @ 63\mod_1554803016350_68.doc...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 130 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0040 Auswahl, Anzeig e und Änderung von D aten @ 27\m od_1405320202914_68.docx @ 193207 @ 4 @ 1 →...
6 Using the centrifuge Pos : 132 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0051 M enü Standard -nur KH S @ 27\mod_1405320205386_68.docx @ 193235 @ 4 @ 1 →...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Runtime The set runtime is displayed in the upper section of this field, with the remaining runtime shown below. The runtime is defined as the period from the start of the centrifuge to the beginning of the deceleration phase. The maximum value is 99 h 59 min 59 sec.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Rotor: rotor selection list This field shows the rotor that is currently being used. • Select the field "Rotor" and confirm the selection. A list with all of the possible rotors without buckets is displayed.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Acceleration This function is used to select an acceleration curve. One can select a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The acceleration curves 20-29 can be programmed as desired (see chapter 11.3 - "Acceleration and deceleration curves").
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Fig. 14: Program "RAPID_TEMP" • Press the start button in order to start the cooling/heating run. • During the cooling/heating run, only the set temperature can be changed. The current status of the program will be displayed on the status bar.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run. For this purpose, a green progress bar and percentage value are displayed in the program field.
6 Using the centrifuge Pos : 136 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0061 M enü Param eter - nur KHS @ 27\m od_1405320221554_68.docx @ 193319 @ 4 @ 1 →...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Radius The radius determines the values of the relative centrifugal force (RCF) that the sample is subjected to. Normally, the maximum RCF value is displayed. If the value is reduced manually, a downward facing arrow "" will be displayed in the RCF field.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Temperature monitoring The control system includes a temperature monitoring function. If the actual temperature difference with regard to the set value deviates from the set temperature difference, the centrifuge will stop and an error message will be issued.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Lock In order to prevent any unauthorised use of the centrifuge, the following functions can be blocked: • Saving of programs (level 01) • Changing of parameters, short run (level 02) •...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Pos : 138 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0070 M enü Setup @ 27\mod_1405320225479_68.docx @ 193361 @ 4 @ 1 →...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Start delay If the start delay function is active, the centrifuge will not start until the preset time has elapsed. The symbol "" will be displayed in the runtime field.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge System Cycles This field shows the number of cycles as well as the runtime of the rotor and buckets that are used. Info This item provides information on the software versions that are used in this centrifuge.
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Pos : 140 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0080 M enü Kurve @ 27\mod_1405320228169_68.docx @ 193389 @ 4 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 6 Using the centrifuge Pos : 144 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0020-0100 Kontr aständerung @ 27\m od_1405320232376_68.docx @ 193431 @ 4 @ 1 →...
6 Using the centrifuge Pos : 149 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0010 Programm speic her n @ 27\m od_1405320237832_68.docx @ 193487 @ 4 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 155 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0040 Programm l ösc hen @ 27\mod_1405320245137_68.docx @ 193557 @ 4 @ 1 →...
6 Using the centrifuge Pos : 157 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0030-0050 Autom atis che Programmrotation @ 27\m od_1405320248329_68.docx @ 193585 @ 4 @ 1 →...
Pos : 159 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/060 Betrieb_Spi nc ontr ol L+S/Spinc ontrol S (Z ent7)/060- 0030-0040 Opti onen z ur Ein- und Ausgabe von D aten (auß er 8KS) @ 27\m od_1405320251483_68.docx...
→ Malfunctions and error correction Pos : 165 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/070 Störungen und F ehl ersuche/070-0013 Allgemei ne Stör ung en_Spi ncontr ol S @ 28\m od_1405320579512_68.docx @ 194705 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 7 Malfunctions and error correction Pos : 167 /100 Sigma/110 BA Z entrifugen Sigma (Proj ekte)/3-18KS-3- 18KH S/070 Störungen und F ehl ers uche/070- 0010-0010 N otentriegel ung 3-18KS_KH S @ 29\mod_1405321826858_68.docx @ 197785 @ 3 @ 1 7.1.1...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 7 Malfunctions and error correction Pos : 169 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/070 Störungen und F ehl ersuche/070-0020 T abell e der F ehlerm eldung en @ 28\mod_1405320582996_68.docx @ 194761 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 7 Malfunctions and error correction Pos : 171 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/070 Störungen und F ehl ersuche/070-0030 Kontak t im Servic efall @ 28\m od_1405320584361_68.docx @ 194775 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 8 Maintenance and service Pos : 174 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080 Wartung und Instandhaltung========================= @ 28\m od_1405320612731_68.docx @ 194789 @ 1 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 191 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020 Sterilisati on und Desinfek tion v on Rotork ammer und Zubehör @ 28\m od_1405320626090_68.docx @ 194929 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 8 Maintenance and service Pos : 193 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0020- 0010 Autoklavi eren @ 28\mod_1405320627440_68.doc x @ 194943 @ 3 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 8 Maintenance and service Pos : 195 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0030 Ins tandhaltungs arbeiten @ 28\mod_1405320628810_68.docx @ 194957 @ 2 @ 1 → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury.
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 8 Maintenance and service Pos : 197 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/080 War tung und Instandhaltung/080-0040 R ücks endung defekter T eile @ 28\mod_1405320630226_68.doc x @ 194971 @ 2 @ 1 →...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 8 Maintenance and service The defective part/unit must be packaged in a transport-safe manner. Please use the original packaging for the unit, if at all possible. If the product is dispatched to us in unsuitable packaging, you will be charged the cost for returning it to you in new packaging.
Pos : 200 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/090 Entsorgung/090 Ents orgung===================================== @ 28\m od_1405320658842_68.docx @ 194985 @ 1 @ 1 → Disposal Pos : 201 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/090 Entsorgung/090-0010 Entsorgung der Z entrifuge---------------------------------------------- @ 28\m od_1405320660029_68.docx @ 194999 @ 2 @ 1 → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left.
10 Technical data Pos : 206 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100 T ec hni sche D aten - Ü bersc hrift ============================= @ 7\mod_1306745782782_68.doc x @ 43932 @ 1 @ 1 →...
Leerz eile @ 0\mod_1202116244500_0.docx @ 114 @ @ 1 Pos : 212 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/100 T ec hnisc he D aten/100- 0020 Technisc he Dok um entation @ 28\m od_1405320681369_68.docx @ 195055 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Pos : 223 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0031 Beschl eunigungs- und Bremsk urven Spinc ontrol S @ 28\mod_1405320708834_68.doc x @ 195181 @ 2 @ 1 →...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Fig. 36: Diagram of linear curves Fig. 37: Diagram of quadratic curves Pos : 224 /010 Univ ers almodul e/Seitenwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1 Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 75 / 89 Translation of the original operating manual, part no.
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Pos : 225 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0040 T abell e: Lebensdauer v on R otoren und Z ubehör @ 28\mod_1405320709911_68.docx @ 195195 @ 2 @ 1 →...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Pos : 227 /100 Sigma/100 BA Z entrifugen Sigma (Standardm odul e)/110 Anhang/110-0050 Beständigkeits tabelle @ 28\mod_1405320711439_68.doc x @ 195209 @ 2 @ 1 → 11.5 Resistance data The data refer to resistance at 20°C.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Pos : 229 /100 Sigma/110 BA Z entrifugen Sigma (Proj ekte)/3-18KS-3- 18KH S/110 Anhang/110-0060 Konformitäts erkl ärung 3- 18KS_KHS @ 29\mod_1405321845824_68.docx @ 197855 @ 2 @ 1 11.6 EC declaration of conformity Version 08/2012, Rev.
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 82 / 89 Translation of the original operating manual, part no. 0701202...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix Declaration of conformity – China RoHS 2 11.7 Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 83 / 89 Translation of the original operating manual, part no. 0701202...
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 11 Appendix === Ende der Liste für T extm arke Inhalt === Version 08/2012, Rev. 1.21 of 08/05/2020 • sb 84 / 89 Translation of the original operating manual, part no. 0701202...
Refrigerated Centrifuge with Heating System Sigma 3-18KHS Index 12 Index Centrifugation with vessels of various sizes 31 Centrifuge cannot be started ...... 56 Acceleration ..........38 Centrifuge decelerates during operation ..56 Acceleration curve ......38, 49, 74 Centrifuge is inclined ........56 Acceleration curve (changing) ....
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Index Dangerous materials ........64 Functional and operating elements .... 11 Date of manufacture ........12 Fuses have tripped ........56 Deactivating the help function ....50 Deceleration (brake) ........38 General conditions ........9 Deceleration curve ......
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Index Layout of the centrifuge......11 Leaks ............65 Online download of forms ......68 Lid cannot be opened ........ 56 Open lid after run ........46 Lid error ............. 58 Opening the lid ........... 28 Lid lock device ...........
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Index Relative centrifugal force (RCF) ... 14, 35 Set-up and connection ....... 26 Removal of a rotor ....... 29, 30 Setup menu ..........46 Removal of the transport safety device ..25 Short run ............ 36 Remove glass particles and metal dust from Solvents .........
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Refrigerated Centrifuge with Heating System Sigma 3-18KHS Index Temperature monitoring ......44 Temperature monitoring system ....22 Unblocking a function ......... 45 Temperature range ........70 Ungreased load- bearing bolts ....56 Temperature unit........47 Useful volume Temperature value not reached ....56 - volume that is stated for the tube ..