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Leica DMS300
User Manual

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Summary of Contents for Leica DMS300

  • Page 1 Leica DMS300 User Manual...
  • Page 2: Table Of Contents

    Incident Light Base and Focusing Column General Instructions Microscope Carrier and Leica DMS300 Important Safety Notes 10" Monitor Symbols Used Illumination: Installing the Leica LED3000 RL Safety Instructions Installation Introduction HD Mode (Standalone) Congratulations! Cables and Terminals, Activating HD Mode (Standalone)
  • Page 3 Displaying Images and Videos Without a Computer Dimensional Drawings White Balance Leica DMS300 PC Mode Leica DMS300 With Incident Light Stand Checking Cables, Terminals and Software Leica DMS300 With Swing-arm Stand Switching on the Leica DMS300 Additional Use of a HD Monitor in PC Mode...
  • Page 4: General Instructions

    General Instructions Leica DMS300 User Manual General Instructions...
  • Page 5: General Instructions

    General Instructions Safety concept Cleaning Servicing Before using your Leica microscope for the first Do not use any unsuitable cleaning agents, Repairs may only be carried out by Leica time, please read the "Safety Concept" booklet chemicals or techniques for cleaning.
  • Page 6: Important Safety Notes

    This operating manual describes the special functions of the Leica  DMS300 digital micro- scope system and contains important instruc- tions for its operational safety, maintenance, and accessories.
  • Page 7: Symbols Used

    Failure to comply can cause the following: Important information Hazards to personnel   This symbol indicates additional informa- Hazards to personnel Functional disturbances damaged tion or explanations that are intended to Functional disturbances damaged instruments provide clarity. instruments Leica DMS300 User Manual General Instructions...
  • Page 8: Safety Instructions

    User Manual, and particularly Refer to "Safety Concept" booklet the safety regulations. Never use the Leica DMS300 or its components Electrical components must be placed at for surgical procedures (such as eye surgery) least 10  cm away from the wall and from since it is not intended for such use.
  • Page 9 Avoid contact with powered electrical The ergonomic optics concept and the design circuits, which can lead to injury. of the Leica DMS300 digital microscope system EC Declaration of Conformity aim to limit the strain on the user to the lowest Refer to "Safety Concept" booklet Transport possible level.
  • Page 10: Introduction

    Introduction Leica DMS300 User Manual Introduction...
  • Page 11: Congratulations

    LED illuminators from Leica at the images or even movies. computer monitor can even be used together. factory. This means that if you use a Leica LED This means you will be perfectly equipped for illuminator, you automatically obtain the best...
  • Page 12: Standard Delivery

    Standard Delivery Standard delivery The Leica  DMS300 digital microscope system's Various functions can be assigned to the hand- standard delivery includes: switch/footswitch (such as taking individual pictures, white balance). 1. Digital Microscope System Leica DMS300 2. Remote control unit, with 2× AAA batteries 3.
  • Page 13: System Overview - Boom Stand Configuration

    System Overview – Boom Stand Configuration This overview shows the Leica  DMS300 in an example application with additional components. 1. HD monitor or computer with LAS EZ software 2. Horizontal arm 3. Cross-member 4. Safety ring 5. Column 6. Baseplate 7.
  • Page 14: Overview Of The Instrument

    1. Card holder for SD card 2. Front button 3. Status light 4. IR receiver, for remote control 5. Zoom knob 6. Click-stop slider 7. Leica DMS300 mounting fixture in the microscope carrier 8. Protective cover 9. HDMI connection 10. USB connection 11. Connection for footswitch 12.
  • Page 15: System Overview - Standard Column Configuration

    System Overview – Standard Column Configuration This overview shows the Leica  DMS300 in an example application with additional components. 1. Leica DMS300 2. Microscope carrier 3. Illumination 4. Focusing column 5. Baseplate 6. HD monitor or computer with LAS software Leica DMS300 User Manual...
  • Page 16: Effective Displayed Section

    1. Live image in 16:9 format, displayed on the HD monitor 2. Captured image in 4:3 format, displayed on the computer or saved to an SD card. Leica DMS300 User Manual Introduction...
  • Page 17: Assembly

    Assembly Leica DMS300 User Manual Assembly...
  • Page 18: Leica Dms300 Swing-Arm Stand

    For safety 2. Fit the serrated lock washer onto the screw. reasons, use two people for this part of the 3. Install the vertical column on the base plate setup. using the screw. Leica DMS300 User Manual Assembly...
  • Page 19: Safety Ring And Horizontal Arm

      The swing-arm stand is designed so 3. Tighten the clamping screw to prevent the tion. It protects the horizontal arm from acci- that the Leica DMS300 can be moved as arm from extending. dentally falling down.   desired.
  • Page 20: 10" Monitor

    1. Install the monitor holder on the vertical arm. 2. Install the monitor holder on the horizontal arm. 2. Fasten the monitor holder to the clamping screw. 3. Fasten the monitor holder to the clamping screw. 4. Reinstall the clamping screw. Leica DMS300 User Manual Assembly...
  • Page 21: Microscope Carrier And Leica Dms300

    The microscope can be rotated freely together the bore of the horizontal arm. with the microscope carrier. 2. Place the Leica  DMS300 in the microscope carrier so that the notch in the microscope 2. Fasten the microscope carrier in place using the washer and safety screw.
  • Page 22: Objective Installation

    Remove all specimens from the stage plate first. 1. Remove the protective cap on the optics 2. Screw the objective into the optics carrier. carrier by turning it. Leica DMS300 User Manual Assembly...
  • Page 23: Installing The Leica Ring Light

    Leica ring light illuminator. illuminator and the locking screw of the optics carrier are on a straight line. 2. Attach the Leica ring light illuminator to the objective from below. 3. Tighten the locking screw. Leica DMS300 User Manual...
  • Page 24 1. The diffuser is attached and screwed in below the ring light illuminator.   We recommend assembling the diffuser on the Leica ring light illuminator on a table in reverse and then mounting them on the objective together. Leica DMS300 User Manual...
  • Page 25: Leica Dms300 With Incident Light Stand

      This chapter shows an example of assem- 1. Take the three provided screws out of the bly of the Leica  DMS300 on an incident packaging. light base. Images and descriptions may vary 2. Install the incident light base on the column when using another incident light base.
  • Page 26: Microscope Carrier And Leica Dms300

    1. Unscrew the clamping screw. using the provided Allen key and the also be installed upside-down (recom- 2. Place the Leica  DMS300 in the microscope provided screw (recommended variant for mended variant for objectives with a large carrier so that the notch in the microscope objectives with a small working distance).
  • Page 27: 10" Monitor

    6. The bracket can be positioned as desired on the back of the column. 3. Rotate the thumb screw so that the mount- ing pins rotate 90° clockwise. Leica DMS300 User Manual Assembly...
  • Page 28: Illumination: Installing The Leica Led3000 Rl

    Ensure that the locking screw of the illu- (10 450 266) to the Leica LED3000 RL. minator and the locking screw of the optics carrier are on a straight line. 2. Attach the Leica LED3000  RL to the objec- tive from below. 3. Tighten the locking screw.  ...
  • Page 29   We recommend assembling the accesso- process. ries on the Leica LED3000 RL on a table in reverse and then mounting them on the objec- tive together. Leica DMS300 User Manual...
  • Page 30: Installation

    Installation Leica DMS300 User Manual Installation...
  • Page 31: Hd Mode (Standalone)

    Leica DMS300 to HD mode (standalone). Leica DMS300.   An SD card with sufficient free memory 1. Connect the Leica  DMS300 to a suitable must be inserted into the Leica DMS300 power socket using the USB cable and in order to save images in HD mode (stand- power supply.
  • Page 32: Pc Mode

    DVD provided with the Leica DMS300. the color of the status light. The status light is red when switching on the Leica  DMS300; as soon as it is green, you can open the software 1. Place the DVD provided into the computer's and view, record or manage images.
  • Page 33: Limitations In Pc Mode

    16:9   Images are always output in a 4:3 aspect ratio when the Leica  DMS300 is connected to a computer – even if a 16:9 moni- tor is connected via HDMI. However, the image is displayed correctly on these kinds of devices, just with black on the screen edges.
  • Page 34: Startup Procedure

    Startup Procedure Leica DMS300 User Manual Startup Procedure...
  • Page 35: Leica Dms300

    Leica DMS300 Switching on the Leica DMS300 HD mode (standalone) PC Mode   If the Leica DMS300 is connected to an HD monitor in PC mode: the remote control does not respond; in other words, the user menu does not appear. On the HD monitor, a live image is shown in 4:3 aspect ratio (with black bars right and left).
  • Page 36: Switching On The Illumination

    Switching on the Illumination General instructions   The light from the Leica LED illuminator can be very bright. Avoid looking directly into the LEDs.   The intensity of the illuminator can be adjusted in 10 increments.   When pressing the buttons, hold the keypad between your thumb and index 1.
  • Page 37: Focusing, Adjusting The Resistance Of The Focus Drive

    1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing. Boom Stand Incident light base Leica DMS300 User Manual Startup Procedure...
  • Page 38: Changing Magnification, Click-Stop Feature

    Continuous zoom is possible when the click stops are disabled, which many users find convenient.   Parfocality: The Leica  DMS300 is parfo- cally matched. If the specimen is in focus at the highest magnification level, this remains 1. Look at the HD monitor.
  • Page 39: Remote Control

    8. Pairing button 9. Show/hide information box Always keep the remote control for controlling 10. White balance set button the Leica  DMS300 pointed towards the micro- 11. Call up the camera menu scope's IR receiver. 12. Arrow keys for navigation  ...
  • Page 40 HD monitor. If this is not the case, the batteries need to be changed.   For the battery replacement, you need two (2) type AAA batteries. Leica DMS300 User Manual Startup Procedure...
  • Page 41: Pairing Cameras With Remotes

    3. The microscope only responds to the remote control commands from the remote   The Leica DMS300 and the remote control used for the pairing. control can be paired and then only respond to each other. This can be helpful when Resetting to factory setting using multiple cameras and remote controls.
  • Page 42: Configuring The Front Button Or Footswitch

    2. Press the button to show the user menu on the computer monitor. 3. Select "SETUP USER" in the main menu: Both the Leica  DMS300's front button and footswitch can be configured with the remote control as defined by the user.  ...
  • Page 43 WHITE BAL: Run white balance for color correcting the camera. For additional infor- mation on white balance, refer to page 50. See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual Startup Procedure...
  • Page 44 Operation Leica DMS300 User Manual Operation...
  • Page 45: Operation

    "HD" (standalone).   An SD card with sufficient free memory 1. Check that the Leica DMS300 is properly must be inserted into the Leica DMS300 supplied with power via the USB power in order to be able to save images in HD mode supply.
  • Page 46: Switching On The Leica Dms300

    1. Set the on/off switch to "ON" to switch on the Leica DMS300. 2. The status light's LED switches from red to green, a signal tone sounds and the live image is displayed on the HD monitor – the Leica DMS300 is ready to use. Leica DMS300 User Manual Operation...
  • Page 47: Information About Sd Memory Cards

    An SD card cannot be formatted in the from being deleted. microscope. Leica DMS300. If it is necessary to format the card, use a computer to do so. You can also If you cannot save any more images to the...
  • Page 48: Capturing Images Without A Computer

    1.3 megabytes of storage top of the Leica DMS300 until it clicks into flashes green and the word "Capture..." flashes space on the SD card. This means that per giga- place.
  • Page 49: Displaying Images And Videos Without A Computer

    Displaying Images and Videos Without a Computer   The Leica  DMS300 can display images 007/0109 IC800007.MP4 007/0109 IC800007.MP4 and video clips directly on an HD monitor. Control via remote control: : Show/hide miniature view. : Select miniature image (JPG or MP4).
  • Page 50: White Balance

    5 seconds. The microscope now carries out mum results when used in conjunction with a an automatic white balance adjustment. Leica LED illuminator. Leica DMS300 User Manual Operation...
  • Page 51: Pc Mode

      Leica Application Suite EZ has to be installed on the computer in order to use the Leica  DMS300 in PC mode. The computer also has to have sufficient free hard drive space.   If you want to perform demanding image...
  • Page 52: Switching On The Leica Dms300

    Switching on the Leica DMS300 Switching on the Leica DMS300 (PC mode)   For additional information on Leica Appli- cation Suite, please refer to "LAS Help". 1. Set the on/off switch to "ON" to switch on the Leica DMS300. 2. Start Leica Application Suite EZ.
  • Page 53: Additional Use Of A Hd Monitor In Pc Mode

    The resolution on the connected HD monitor is limited by the live image reso- lution of the LAS EZ software. Due to technical 1. Connect the Leica DMS300 to the HD moni- limitations, it is not possible to display a Full tor using the HDMI cable.
  • Page 54: User Menu

    User Menu Leica DMS300 User Manual User Menu...
  • Page 55: Calling Up The User Menu

    5. Press the button again to hide the user menu. Check to ensure that the Leica DMS300 is in HD mode and that a live image is shown on a connected HD monitor. See more on this topic in our online videos at our website www.leica-microsystems.com.
  • Page 56: Automatic White Balance

    For transmitted light specimens, configuring the white balance to the pure transmitted light at moderate brightness is recommended (without the specimen). See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 57: Manual White Balance

    The white balance mode is always set to "MANUAL" after manually adjusting the white balance, even if it was set to "AUTO" previously. See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 58: Exposure

    3. Set the value for "EXP MODE" to "AUTO" for automatic exposure. 4. Correct the values for "BRIGHTNESS" and "GAMMA" until you obtain the desired results for the image. Main menu Submenu See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 59: Resolution

      The "RESOLUTION" menu lets you define the resolution individually for the live image on the computer, capturing individual images and capturing video. This provides optimum results in every situation. Leica DMS300 User Manual User Menu...
  • Page 60 SD card runs out of memory.   A double signal tone is output when activating the recommended default resolution of HD1080-50. See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 61: Camera Settings

    Resets the microscope to the factory default settings. All user information video recordings. (such as white balance, resolution, etc.) is reset. Select this function if you are getting the impression the microscope is not responding normally or a setting has not been made correctly. Leica DMS300 User Manual User Menu...
  • Page 62 If you save the file name or the date and time into images, they cannot be deleted later, even if you rename the files or change the date. See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 63: User-Defined Settings

    Currently, two color schemes are available for the menu guidance. Addi- enjoyable experience. tional colors may be provided in the future via a firmware update. Leica DMS300 User Manual User Menu...
  • Page 64 The menu entry for selecting the language is always added in English as well. See more on this topic in our online videos at our website www.leica-microsystems.com. Leica DMS300 User Manual User Menu...
  • Page 65: Setting The Overlay (Setup Overlay)

    Various overlays are available for download on the content and let you configure your microscope for special tasks. Leica Microsystems website, as well as a manual for how to create your own overlays. Follow those instructions to create new overlays.
  • Page 66 Setting the Overlay (Continued) Restore an overlay (RESTORE OVERLAY) Resets the overlays to the factory setting: A histogram, a Leica logo, a cross- hair over the entire live image and a small crosshair in the middle of the live image.
  • Page 67: Configuring Predefined Illumination Scenarios

    Configuring Predefined Illumination Scenarios Changing the illumination mode Sequence     The Leica DMS300 is supplied with vari- To return to the last selected mode, press ous illumination modes that cover a wide until the "LAST USED" setting spectrum of possible situations.
  • Page 68: Service

    Service Leica DMS300 User Manual Service...
  • Page 69: Care, Maintenance, Contact Persons

    Leica representatives on the Leica Microsystems ing tips will ensure that even after years and website: www.leica-microsystems.com. decades, your Leica microscope will continue to work as well as it did on the very first day. Leica DMS300 User Manual Service...
  • Page 70 Do not use alcohol. parts. Avoid excessive use of solvents. The lint-free cloth, lens cloth or cotton swab should be soaked with solvent, but not so wet that solvent runs over the lens. Leica DMS300 User Manual Service...
  • Page 71 Servicing Some components are made of polymer or are Clean the microscope (or parts of it) using Repairs may only be carried out by Leica polymer-coated. They are, therefore, pleasant warm soapy water, then wipe using distilled Microsystems-trained service technicians.
  • Page 72: Spare Parts

    USB cable (1.8 m) 13 302 708 946 915 5 W USB adapter with interchangeable connector 19 003 658 25 W power supply for Leica ring light illuminator 10 450 805 IR remote control RC3 19 004 870 SD card (4 GB)
  • Page 73: Specifications

    Specifications Leica DMS300 User Manual Specifications...
  • Page 74: Technical Data

    Leica DMS300@ highest lowest zoom position zoom position Resolution 142 p/mm 24 lp/mm FoVx 4.06 mm 32.44 mm FoVy 2.28 mm 18.25 mm 0.178 mm 3.448 mm Max@22"-monitor 119.3 × 15.1 × * With 10" monitor Leica DMS300 User Manual Specifications...
  • Page 75 HD ready: 1,280×720 – 50 Hz/60 Hz – 30 fps Supported operating systems Windows XP, Windows 7, Mac OS X Leica Software PC: Leica LAS & LAS EZ software Full HD: 1,920×1,080 – Mac: Leica Acquire Software 50 Hz/60 Hz/25 Hz/30 Hz – 30 fps...
  • Page 76 SD HC compatible, 128 MB – 32 GB Status LED 3 colors: green – power on, yellow – busy, red – error Power supply Via USB cable to computer or to external 5 V USB power supply unit Power requirement Leica DMS300 User Manual Specifications...
  • Page 77: Optical Data

    1.25 27.3 1.45 34.9 0.97 27.6 0.97 4.06 2.28 43.6 0.69 34.5 0.69 24" 19" 54.5 0.50 43.2 0.50 69.8 0.36 55.3 0.36 87.2 0.28 69.1 0.28 109.0 0.22 86.4 0.22 130.8 0.18 103.6 0.18 Leica DMS300 User Manual Specifications...
  • Page 78: Dimensional Drawings

    Dimensional Drawings Leica DMS300 LEI C A DMS 300 LEICA DMS 300 M60x1 Leica DMS300 User Manual Specifications...
  • Page 79: Leica Dms300 With Incident Light Stand

    Leica DMS300 With Incident Light Stand LEICA DMS 1000 LEICA DMS 1000 Leica DMS300 User Manual Specifications...
  • Page 80: Leica Dms300 With Swing-Arm Stand

    Leica DMS300 With Swing-arm Stand LEICA DMS 1000 LEICA DMS 300 Leica DMS300 User Manual Specifications...
  • Page 81 CONNECT WITH US! Leica Microsystems (Schweiz) AG · Max-Schmidheiny-Strasse 201 · 9435 Heerbrugg, Switzerland T +41 71 726 34 34 · F +41 71 726 34 44 www.leica-microsystems.com...

Table of Contents