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Leica DM750
Manual

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Summary of Contents for Leica DM750

  • Page 1 Leica DM750 Manual...
  • Page 2 Manufacturer information Published in November 2010 by: Leica Microsystems (Schweiz) AG Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) Responsible for contents: Dietmar Gnass (Product Compliance) Vincent Vaccarelli (Product Management) In case of questions, please contact the hotline: mqm-hotline@leica-microsystems.com...
  • Page 3: Table Of Contents

    Chapter overview Safety regulations The Leica DM750 Ready! Set! Care of the Microscope Troubleshooting Dimensions Leica DM750 Manual...
  • Page 4 Care of the Microscope Unpacking General maintenance Ready! Troubleshooting Substage Illumination Troubleshooting Attaching the viewing Tubes Leica EZ Viewing Tube – integrated eyepieces Dimensions Leica Standard Viewing Tube – separated eyepieces Eyecups Installing Objectives Installing the Substage Condenser Leica DM750 Manual...
  • Page 5: Safety Regulations

    Safety regulations Leica DM750 Manual...
  • Page 6: Safety Concept

    This user manual describes the special func- tions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety, main- tenance, and accessories.
  • Page 7: Symbols Used In This Instruction Manual

    Instructions for disposing of the inst- Failure to comply can cause the following: rument, accessory components, and Hazards to personnel consumables. Ϙ Functional disturbances or damaged Ϙ instruments Leica DM750 Manual...
  • Page 8: Important Notes

    Important Notes Description User Manual Accessories from third-party suppliers The Leica DM750 microscope meets today's This user manual includes important instruc- You can combine individual system articles with state of the art of technology. Nevertheless, tions related to operating safety, maintenance articles from external suppliers.
  • Page 9: Intended Purpose Of The Microscope

    Leica ronment as defined by DIN VDE 0100-710. research applications. This includes exami-...
  • Page 10: Instructions On Use

    If using the devices outdoors, protect so in the instructions. The Leica DM750 microscope can be used in them from dust and moisture. Never use electri- clean rooms without any problems. cal devices outdoors. Install electrical devices at  ...
  • Page 11 Once the product has reached the end of its When installing Leica products into third-party altered, modified or used in conjunction with service life, please contact Leica Service or Sales products, the manufacturer of the complete non-Leica components that are outside of the about disposal.
  • Page 12: Health Risks And Dangers Of Use

    Dangers during use   Workplaces with microscopes facilitate Leica microscopy series are intended to reduce The Leica DM750 microscope may only be Ϙ and improve the viewing task, but they the exertion of the user to a minimum. connected to a grounded socket.
  • Page 13: Information For The Person Responsible For The Instrument

    Information for the person responsible for the instrument Information for the person responsible for Do not use the Leica DM750 microscope Only original Leica replacement parts may Ϙ Ϙ the instrument unless it is in perfect condition. be used in servicing the product.
  • Page 14: Care Instructions

    Remove coarse debris with a moistened Ϙ disposable cloth. Remove dirt using a clean cloth that has Ϙ Protect the Leica DM750 microscope from been moistened with distilled water. Ϙ oil and grease. Never grease or oil mechani- Clinging dirt can be removed using petro- Ϙ...
  • Page 15: Accessories, Maintenance And Repair

    Accessories, maintenance and repair Accessories Maintenance Repairs and service work Only the following accessories may be used The Leica DM750 microscope is basically Only original Leica Microsystems spare Ϙ Ϙ with the Leica DM750 microscope: maintenance-free. To ensure that it always parts may be used.
  • Page 16: Electrical Data And Ambient Conditions

    In order to maintain this condition and to ensure safe operation,   Unplug the instrument before changing any fuses. The Leica the user must follow the instructions and warnings contained in DM750 has two fuses, which are located behind the power cord this instruction manual. receptacle.  ...
  • Page 17: The Leica Dm750

    The Leica DM750 Leica DM750 Manual...
  • Page 18: Introduction

    Introduction Thank you for purchasing the Leica DM750 Compound Microscope from Leica Microsys- tems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument for educatio- nal and routine clinical applications.   The instrument is to be used only as described.
  • Page 19: Unpacking

    These items are delivered in separate packages. Please do not discard any of the packing Ϙ materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM750 Manual...
  • Page 20: Ready

    Ready! Leica DM750 Manual...
  • Page 21: Substage Illumination

    Substage Illumination Substage Illumination Type 1: Standard illumination Type 2: Koehler illumination   The Leica DM750 is available with two Adjustable condenser centering with provided Adjustable condenser centering with types of Substage Illumination. Identify tool. thumbscrews and adjustable Koehler field which type of Illumination you have, as this will diaphragm.
  • Page 22: Attaching The Viewing Tubes

    Allen key provided. Allen key Ϙ 2. Set the dovetail into the tripod mount and Type 1: Leica EZ Viewing Tube with integrated tighten the setscrew carefully (without eyepieces. using excessive force). This precisely posi- tions the viewing tube onto the optical axis of the microscope, regardless of the selec- ted rotation.
  • Page 23: Leica Ez Viewing Tube - Integrated Eyepieces

    Leica EZ Viewing Tube – integrated eyepieces 1. In order to use the captive wingscrew, 2. Reinstall the Leica EZ Viewing Tube onto remove the setscrew that was delivered the stand. with the stand. Leica EZ viewing tube with integrated eyepieces.
  • Page 24 Leica EZ Viewing Tube – integrated eyepieces (cont.)   You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again.   The eyepieces are integrated into the Leica EZ Viewing Tube and preset;...
  • Page 25: Leica Standard Viewing Tube - Separated Eyepieces

    Leica Standard Viewing Tube – separated eyepieces 1. Insert the eyepieces into the tubes. 2. Secure the eyepieces in the tubes by tighte- ning the silver screws at the bottom of the tubes using a standard Phillips screwdriver (not included in the delivery package).
  • Page 26: Eyecups

    In this case, go to section "Operation" on page 30. If you purchased your Leica DM750 in the form of individual components rather than the stan- dard configuration, please continue with the "Installing Objectives"...
  • Page 27: Installing Objectives

    When rotating the objective nosepiece, always use the knurled ring on the objective nosepiece. As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification. Leica DM750 Manual...
  • Page 28: Installing The Substage Condenser

    1. Move the specimen stage upwards as far as 3. Unscrew the two setscrews (or wings-   The Leica DM750 has an open substage it will go by using the coarse focusing knob crews for Koehler stands) on the condenser condenser mount, therefore the conden- on the side of the microscope.
  • Page 29   You will center the condenser more accu- rately when you get to the "Complete Condenser Centering" section on page 32. Leica DM750 Manual...
  • Page 30: Set

    Set! Leica DM750 Manual...
  • Page 31: Turning On The Microscope

    2. Switch on the microscope using the switch Power Cord   system. at the bottom right of the microscope If the power cord is not already atta- stand. ched, attach it securely to the back of the microscope. Leica DM750 Manual...
  • Page 32: Complete Condenser Centering

    3. Look at the X through the eyepieces and center it in the field of view by tightening the setscrews with the tool included in the Centered delivery package (or, for a Leica DM750 with Koehler illumination, tighten the   knurled screws).
  • Page 33: Using The Condenser

    Match the line of the rotating ring with the objective magnification in use. Leica DM750 Manual...
  • Page 34: Prepare To View A Specimen Slide

    2. Using the X/Y-stage control, position the stage by sliding it under the slide grips. specimen slide such that a part of the speci- men is under the objective used.   Slide grips hold the slide in place. Slide grips Leica DM750 Manual...
  • Page 35: Focusing

      The stand of the Leica DM750 has been calibrated at the factory so that the focus can be found from this position within 1.5 rota- tions of the fine focus.
  • Page 36: Viewing Tube Adjustment

    Viewing Tube Adjustment Adjust the eyetubes Leica EZ Viewing Tube If you have a Leica DM750 with stan- Ϙ 1. Adjust the tubes to your interpupillary If you are using a Leica EZ viewing tube, dard illumination (without Koehler field Ϙ...
  • Page 37   When doing so, do not change the If you use a DM750 with standard illumi- Ϙ height of the specimen stage. nation, continue with the "Oil Immersion Technique" section on page 40.
  • Page 38: Koehler Configuration

    Koehler Configuration   If your Leica DM750 is fitted with a field diaphragm for Koehler Illu- 2. Focus the leaves of the field diaphragm using the condenser focusing mination, use the following procedure to assure condenser cente- knob on the left side of the stage mount.
  • Page 39 Koehler Configuration (cont.) 3. Turn the condenser centering wingscrews simultaneously to center 4. Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm. of the field of view. Leica DM750 Manual...
  • Page 40: Oil Immersion Technique

    Oil Immersion Technique 1. Search for the area on the specimen slide 3. Add a drop of immersion oil from Leica 4. Rotate the oil immersion objective (the that you want to study. to the area of the specimen slide you are objective labeled "OIL") to the working...
  • Page 41 Then bring the oil objective into the final position, so that the oil drop is between the front lens of the objective and the specimen slide. Leica DM750 Manual...
  • Page 42: Time Delay Shutoff

    Time Delay Shutoff   The Leica DM750 is equipped with a time Changing the status of the time delay The LED Illumination will flash to indicate Ϙ delay shutoff capability, which automati- shutoff the time delay shutoff status was changed.
  • Page 43 Leica DM750 Manual...
  • Page 44: Ready! Set! Go

    Ready! Set! Go! Now all you need to do is change objectives, set the condenser aperture (and field diaphragm if you have a DM750 Koehler Stand) appropriately for the objective magnification you are using, and enjoy the view! Leica DM750...
  • Page 45: Care Of The Microscope

    Care of the Microscope Leica DM750 Manual...
  • Page 46: General Maintenance

    The microscope should always be covered Ϙ with the plastic dust cover (provided with the instrument) when it is not in use. Leica DM750 Manual...
  • Page 47 The surface of this front lens can be readily cleaned with a toothpick cove- The Leica DM750 has a durable finish. All Ϙ red with a cotton tip, or with a small cotton non-optical parts of the instrument can swab.
  • Page 48: Troubleshooting

    Troubleshooting Leica DM750 Manual...
  • Page 49: Troubleshooting

    Check the condenser centering. Open the aperture diaphragm completely. The image is not uniformly illuminated. The objective magnification is too weak. Use a higher magnification. Unwanted stray light. Clean the specimen and neighboring lens surfaces. Polarization Leica DM750 Manual...
  • Page 50 Then move the mechanical stage with coaxial drive all the tion decreases after working a long time. way to the right. Manually press the screw that holds the mechanical stage even farther to the right, as far as it will go. Leica DM750 Manual...
  • Page 51: Dimensions

    Dimensions Leica DM750 Manual...
  • Page 52 Leica DM750 Manual...
  • Page 53 Leica DM750 Manual...
  • Page 54 Leica DM750 Manual...

Table of Contents