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Manufacturer information Published in November 2010 by: Leica Microsystems (Schweiz) AG Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) Responsible for contents: Dietmar Gnass (Product Compliance) Vincent Vaccarelli (Product Management) In case of questions, please contact the hotline: mqm-hotline@leica-microsystems.com...
This user manual describes the special func- tions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety, main- tenance, and accessories.
Instructions for disposing of the inst- Failure to comply can cause the following: rument, accessory components, and Hazards to personnel consumables. Ϙ Functional disturbances or damaged Ϙ instruments Leica DM750 Manual...
Important Notes Description User Manual Accessories from third-party suppliers The Leica DM750 microscope meets today's This user manual includes important instruc- You can combine individual system articles with state of the art of technology. Nevertheless, tions related to operating safety, maintenance articles from external suppliers.
If using the devices outdoors, protect so in the instructions. The Leica DM750 microscope can be used in them from dust and moisture. Never use electri- clean rooms without any problems. cal devices outdoors. Install electrical devices at ...
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Once the product has reached the end of its When installing Leica products into third-party altered, modified or used in conjunction with service life, please contact Leica Service or Sales products, the manufacturer of the complete non-Leica components that are outside of the about disposal.
Dangers during use Workplaces with microscopes facilitate Leica microscopy series are intended to reduce The Leica DM750 microscope may only be Ϙ and improve the viewing task, but they the exertion of the user to a minimum. connected to a grounded socket.
Information for the person responsible for the instrument Information for the person responsible for Do not use the Leica DM750 microscope Only original Leica replacement parts may Ϙ Ϙ the instrument unless it is in perfect condition. be used in servicing the product.
Remove coarse debris with a moistened Ϙ disposable cloth. Remove dirt using a clean cloth that has Ϙ Protect the Leica DM750 microscope from been moistened with distilled water. Ϙ oil and grease. Never grease or oil mechani- Clinging dirt can be removed using petro- Ϙ...
Accessories, maintenance and repair Accessories Maintenance Repairs and service work Only the following accessories may be used The Leica DM750 microscope is basically Only original Leica Microsystems spare Ϙ Ϙ with the Leica DM750 microscope: maintenance-free. To ensure that it always parts may be used.
In order to maintain this condition and to ensure safe operation, Unplug the instrument before changing any fuses. The Leica the user must follow the instructions and warnings contained in DM750 has two fuses, which are located behind the power cord this instruction manual. receptacle. ...
Introduction Thank you for purchasing the Leica DM750 Compound Microscope from Leica Microsys- tems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument for educatio- nal and routine clinical applications. The instrument is to be used only as described.
These items are delivered in separate packages. Please do not discard any of the packing Ϙ materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM750 Manual...
Substage Illumination Substage Illumination Type 1: Standard illumination Type 2: Koehler illumination The Leica DM750 is available with two Adjustable condenser centering with provided Adjustable condenser centering with types of Substage Illumination. Identify tool. thumbscrews and adjustable Koehler field which type of Illumination you have, as this will diaphragm.
Allen key provided. Allen key Ϙ 2. Set the dovetail into the tripod mount and Type 1: Leica EZ Viewing Tube with integrated tighten the setscrew carefully (without eyepieces. using excessive force). This precisely posi- tions the viewing tube onto the optical axis of the microscope, regardless of the selec- ted rotation.
Leica EZ Viewing Tube – integrated eyepieces 1. In order to use the captive wingscrew, 2. Reinstall the Leica EZ Viewing Tube onto remove the setscrew that was delivered the stand. with the stand. Leica EZ viewing tube with integrated eyepieces.
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Leica EZ Viewing Tube – integrated eyepieces (cont.) You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again. The eyepieces are integrated into the Leica EZ Viewing Tube and preset;...
Leica Standard Viewing Tube – separated eyepieces 1. Insert the eyepieces into the tubes. 2. Secure the eyepieces in the tubes by tighte- ning the silver screws at the bottom of the tubes using a standard Phillips screwdriver (not included in the delivery package).
In this case, go to section "Operation" on page 30. If you purchased your Leica DM750 in the form of individual components rather than the stan- dard configuration, please continue with the "Installing Objectives"...
When rotating the objective nosepiece, always use the knurled ring on the objective nosepiece. As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification. Leica DM750 Manual...
1. Move the specimen stage upwards as far as 3. Unscrew the two setscrews (or wings- The Leica DM750 has an open substage it will go by using the coarse focusing knob crews for Koehler stands) on the condenser condenser mount, therefore the conden- on the side of the microscope.
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You will center the condenser more accu- rately when you get to the "Complete Condenser Centering" section on page 32. Leica DM750 Manual...
2. Switch on the microscope using the switch Power Cord system. at the bottom right of the microscope If the power cord is not already atta- stand. ched, attach it securely to the back of the microscope. Leica DM750 Manual...
3. Look at the X through the eyepieces and center it in the field of view by tightening the setscrews with the tool included in the Centered delivery package (or, for a Leica DM750 with Koehler illumination, tighten the knurled screws).
2. Using the X/Y-stage control, position the stage by sliding it under the slide grips. specimen slide such that a part of the speci- men is under the objective used. Slide grips hold the slide in place. Slide grips Leica DM750 Manual...
The stand of the Leica DM750 has been calibrated at the factory so that the focus can be found from this position within 1.5 rota- tions of the fine focus.
Viewing Tube Adjustment Adjust the eyetubes Leica EZ Viewing Tube If you have a Leica DM750 with stan- Ϙ 1. Adjust the tubes to your interpupillary If you are using a Leica EZ viewing tube, dard illumination (without Koehler field Ϙ...
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When doing so, do not change the If you use a DM750 with standard illumi- Ϙ height of the specimen stage. nation, continue with the "Oil Immersion Technique" section on page 40.
Koehler Configuration If your Leica DM750 is fitted with a field diaphragm for Koehler Illu- 2. Focus the leaves of the field diaphragm using the condenser focusing mination, use the following procedure to assure condenser cente- knob on the left side of the stage mount.
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Koehler Configuration (cont.) 3. Turn the condenser centering wingscrews simultaneously to center 4. Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm. of the field of view. Leica DM750 Manual...
Oil Immersion Technique 1. Search for the area on the specimen slide 3. Add a drop of immersion oil from Leica 4. Rotate the oil immersion objective (the that you want to study. to the area of the specimen slide you are objective labeled "OIL") to the working...
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Then bring the oil objective into the final position, so that the oil drop is between the front lens of the objective and the specimen slide. Leica DM750 Manual...
Time Delay Shutoff The Leica DM750 is equipped with a time Changing the status of the time delay The LED Illumination will flash to indicate Ϙ delay shutoff capability, which automati- shutoff the time delay shutoff status was changed.
Ready! Set! Go! Now all you need to do is change objectives, set the condenser aperture (and field diaphragm if you have a DM750 Koehler Stand) appropriately for the objective magnification you are using, and enjoy the view! Leica DM750...
The microscope should always be covered Ϙ with the plastic dust cover (provided with the instrument) when it is not in use. Leica DM750 Manual...
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The surface of this front lens can be readily cleaned with a toothpick cove- The Leica DM750 has a durable finish. All Ϙ red with a cotton tip, or with a small cotton non-optical parts of the instrument can swab.
Check the condenser centering. Open the aperture diaphragm completely. The image is not uniformly illuminated. The objective magnification is too weak. Use a higher magnification. Unwanted stray light. Clean the specimen and neighboring lens surfaces. Polarization Leica DM750 Manual...
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Then move the mechanical stage with coaxial drive all the tion decreases after working a long time. way to the right. Manually press the screw that holds the mechanical stage even farther to the right, as far as it will go. Leica DM750 Manual...