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Leica DM750 M
User Manual

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Summary of Contents for Leica DM750 M

  • Page 1 Leica DM750 M User Manual...
  • Page 2: Manufacturer Information

    Manufacturer Information Published in October 2019 by: Leica Microsystems (Schweiz) AG Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) In case of questions, please contact the hotline: sms.support@leica-microsystems.com Leica DM750 M User Manual...
  • Page 3: Table Of Contents

    Chapter Overview Safety Regulations Assembling the Leica DM750 M Conversion and Use with Transmitted Light Care of the Microscope Leica DM750 M User Manual...
  • Page 4 Assembling the Leica DM750 M Focusing Assembling the Incident Light Axis Viewing Tube Adjustment Assembling the Viewing Tubes Koehler Configuration Leica EZ Viewing Tube with Integrated Eyepieces Leica Standard Tube with Separate Eyepieces Care of the Microscope Eyecups General Maintenance Installing Objectives...
  • Page 5: Safety Regulations

    Safety Regulations Leica DM750 M User Manual Safety Regulations...
  • Page 6: Safety Concept

    Safety Concept The individual modules of the Leica DM micros- You can combine individual system articles with copy series include an interactive CD-ROM with articles from external suppliers. Please read the all relevant user manuals in several languages. user manual and the safety requirements of the Keep it in a safe place, and readily accessible supplier.
  • Page 7: Symbols Used In This Instruction Manual

    Instructions for disposing of the Failure to comply can cause the following: instrument, accessory components, Hazards to personnel and consumables. Functional disturbances or damaged instruments Leica DM750 M User Manual Safety Regulations...
  • Page 8: Important Notes

    Important Notes Description User manual Accessories from third-party suppliers The Leica DM750 M microscope meets today's This user manual includes important instruc- You can combine individual system articles with state of the art of technology. Nevertheless, tions related to operating safety, maintenance articles from external suppliers.
  • Page 9: Instructions On Use

    Instructions on Use   The Leica DM750 M microscope may be Place of use Non-intended use   used only in closed rooms and must be Only use the instruments in closed, dust free Never install any other plug or unscrew placed on a solid substrate.
  • Page 10 Once the product has reached the end of its When installing Leica products into third-party altered, modified or used in conjunction with service life, please contact Leica Service or Sales products, the manufacturer of the complete non-Leica components that are outside of the about disposal.
  • Page 11: Health Risks And Dangers Of Use

    Danger of infection holding muscles of the user. Depending on the The Leica DM750 M microscope may not be   duration of uninterrupted work, asthenopia Direct contact with eyepieces is a operated unless it is in proper functioning and musculoskeletal problems may occur.
  • Page 12: Information For The Person Responsible For The Instrument

    Information for the Person Responsible for the Instrument Information for the person responsible for Do not use the Leica DM750 M microscope Only original Leica replacement parts may the instrument unless it is in perfect condition. be used in servicing the product.
  • Page 13: Care Instructions

    Care Instructions General instructions Cleaning coated parts and plastic parts Cleaning glass surfaces Protect the Leica DM750 M microscope Dust and dirt particles should be removed Remove dust using a dry and grease-free against damp, vapors, acids, alkalis, and with a soft brush or lint-free cotton cloth.
  • Page 14: Accessories, Maintenance And Repair

    Accessories Maintenance Repairs and service work Only the following accessories may be used The Leica DM750 M microscope is basically Only original Leica Microsystems spare with the Leica DM750 M microscope: maintenance-free. To ensure that it always parts may be used.
  • Page 15: Electrical Data And Ambient Conditions

      Unplug the instrument before changing any fuses. The Temperature for use +10 °C … +40 °C Leica DM750 M includes two fuses that are located behind Storage temperature -20 °C … +52 °C the power cable compartment. +50 °F … +104 °F  ...
  • Page 16: Assembling The Leica Dm750 M

    Assembling the Leica DM750 M Leica DM750 M User Manual Assembling the Leica DM750 M...
  • Page 17: Assembling The Incident Light Axis

    1. Place the incident light axis with the heat tightening the setscrew. sink facing backwards onto the tripod mount. 2. Align the incident light axis according to the tripod geometry. Leica DM750 M User Manual Assembling the Leica DM750 M...
  • Page 18: Assembling The Viewing Tubes

    Allen key provided. the tube automatically shifts to the correct position on the optical axis of the micro- scope. Type 1: Leica EZ viewing tube with integrated eyepieces Type 2: Standard tube with separate eyepieces Leica DM750 M User Manual...
  • Page 19: Leica Ez Viewing Tube With Integrated Eyepieces

    Leica EZ Viewing Tube with Integrated Eyepieces 1. In order to use the wingscrew, remove the 3. Reinstall the Leica EZ viewing tube onto the setscrew that was delivered with the stand. stand. Leica EZ viewing tube with integrated eyepieces  ...
  • Page 20 Leica EZ Viewing Tube – Integrated Eyepieces (Continued)   Now, you can rotate the Leica EZ viewing tube by loosening the wingscrew, bring- ing the tube to the desired position and tight- ening the wingscrew again.   The eyepieces are integrated into the Leica EZ Viewing Tube and preset;...
  • Page 21: Leica Standard Tube With Separate Eyepieces

    Leica Standard Tube with Separate Eyepieces 1. Insert the eyepieces into the tubes. 2. Secure the eyepieces in the tubes by tight- ening the silver screws at the bottom. Standard tubes; tubes do not include eyepieces   The standard tube includes a rotatable dovetail.
  • Page 22: Eyecups

    In this case, continue with the room light. "Use" section starting on page 25. If you have purchased your Leica DM750 M in individual components and not according to the standard configuration, continue with the "Installing Objectives" section on page 23.
  • Page 23: Installing Objectives

    When rotating the objective nosepiece, always use the knurled ring on the objective nosepiece. While rotating the objective nosepiece clock- wise, screw the objectives into the objective nosepiece. Start with the lowest magnification. Leica DM750 M User Manual Assembling the Leica DM750 M...
  • Page 24: Conversion To Incident Light Polarization

    This will automatically shut off the reflec- ted light to save energy and avoid any false pleochroism effect that could be caused by the beam splitter. Leica DM750 M User Manual Assembling the Leica DM750 M...
  • Page 25: Use

    Leica DM750 M User Manual...
  • Page 26: Switching On The Microscope

    Precautionary measures 2. Switch on the illumination by briefly pres- The brightness can be adjusted in 15   The light of the Leica DM750 M can be sing the power switch. increments. very bright. Therefore, only look into the eyepieces after you have switched on and...
  • Page 27: Modifying The Aperture Diaphragm

      The aperture diaphragm must be completely open for all oblique light illu- mination scenarios. Leica DM750 M User Manual...
  • Page 28: Illumination Scenarios

    (oblique incident illumination). oblique illumination. Combinations with other light fields are also possible.   The aperture diaphragm must be completely open for all oblique light illu- mination scenarios. HOLD Bright field illumination Individual light field Oblique illumination Leica DM750 M User Manual...
  • Page 29 HOLD Any arrangement Switching between bright field illumination Switching automatically and the most recent setting between the light fields Leica DM750 M User Manual...
  • Page 30: Automatic Shutoff Of Illumination

    Automatic Shutoff of Illumination   The illumination of the Leica DM750 M Deactivating delayed shutoff Activating delayed shutoff is shut off automatically if the user does 1. Switch the incident light axis off. 1. Switch the incident light axis off.
  • Page 31: Using Specimen Holders

    13 613 167 Order number for 30 mm version: 13 613 168   The maximum height of the specimen is 5. Position the specimen by moving the stage 30 mm. and looking through the eyepieces simulta- neously. Leica DM750 M User Manual...
  • Page 32: Conversion And Use With Transmitted Light

    Conversion and Use with Transmitted Light Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 33: Optional Koehler Illumination

    Optional Koehler Illumination Transmitted illumination Type 1: Standard condenser Type 2: Koehler condenser   For the Leica DM750 M, there are two Adjustable condenser centering with tool kept Adjustable condenser centering with condensers available for optimum trans- at the ready: wingscrews and adjustable Koehler field mitted illumination.
  • Page 34: Installing The Condenser

    1. Move the specimen stage upwards as far as 3. Unscrew the two setscrews (or wingscrews   The Leica DM750 M is equipped with it will go using the coarse focusing knob. for Koehler stands) on the condenser an open holder for the specimen stage holder.
  • Page 35   How to center the condenser more precisely is described in the section entitled "Complete Condenser Centering" on page 37. Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 36: Switching On The Microscope

    USB power connector The Leica DM750 M has a 5 V/1.5 A USB power connector in the center of the cord wrap. This can be used to power some Leica cameras, incident illuminator, or other devices requiring 4.
  • Page 37: Complete Condenser Centering

    3. Look through the eyepieces and center the Centered "X" in the field of view by tightening the setscrews. (Leica DM750 M with Koehler illumination: tighten the knurled screws). Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 38: Using The Condenser

    The line on the ring is aligned with the objective magnification used. Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 39: Preparation For Viewing A Specimen Slide

    2. Position the specimen slide such that a stage by sliding it under the slide grips. part of the specimen is under the objective used.   Slide grips hold the slide in place. Slide grips Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 40: Focusing

      The stand of the Leica DM750 M has been calibrated at the factory so that the focus can be found from this position within 1.5 rota- tions of the fine focus.
  • Page 41: Viewing Tube Adjustment

    Leica EZ viewing tube Standard viewing tube with two fixed 1. Adjust the tubes to your interpupillary If you are using a Leica EZ viewing tube, eyepieces distance. Fold or unfold the eyetubes to which has eyepieces that are integrated...
  • Page 42   If you are comfortable wearing your the non-focusable eyepiece. corrective lenses (contact lenses or eyeglasses) for microscope viewing, leave them on and your adjustments will be minimal. Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 43 If your DM750 M is equipped with a Koeh- ler configuration, continue with the next section entitled "Koehler Configuration" on page 44.
  • Page 44: Koehler Configuration

    Koehler Configuration   If your Leica DM750 M is equipped with a field diaphragm for 2. Focus the illuminated field diaphragm using the condenser focusing Koehler illumination, center the condenser as described below. knob on the left side of the stage mount.
  • Page 45 3. Turn the condenser centering wingscrews simultaneously to center 4. Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm. of the field of view. Leica DM750 M User Manual Conversion and Use with Transmitted Light...
  • Page 46: Care Of The Microscope

    Care of the Microscope Leica DM750 M User Manual Care of the Microscope...
  • Page 47: General Maintenance

    The microscope should always be covered with the plastic dust cover (provided with the instrument) when it is not in use. Leica DM750 M User Manual Care of the Microscope...
  • Page 48 Illumination tion objectives, the objective has a small The Leica DM750 M uses LED illumination. concave front lens of fairly short radius or Therefore, no lamp changing is required for curvature. The surface of this front lens can the life of the microscope.
  • Page 49 CONNECT WITH US! Leica Microsystems (Schweiz) AG · Max-Schmidheiny-Strasse 201 · 9435 Heerbrugg, Switzerland T +41 71 726 34 34 · F +41 71 726 34 44 www.leica-microsystems.com...

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