Leica DM750 User Manual
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Table of Contents
Troubleshooting
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Summary of Contents for Leica DM750
- Page 1 Leica DM750 User Manual...
- Page 2 Manufacturer Information Published in January 2015 by: Leica Microsystems (Schweiz) AG Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) In case of questions, please contact the hotline: sms.support@leica-microsystems.com Leica DM750 User Manual...
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Page 3: Table Of Contents
Chapter Overview Safety Regulations The Leica DM750 Ready! Set! Care of the Microscope Troubleshooting Dimensions Leica DM750 User Manual... - Page 4 Care of the Microscope Ready! General Maintenance Substage Illumination Troubleshooting Attaching the Viewing Tubes Troubleshooting Leica EZ Viewing Tube – Integrated Eyepieces Leica Standard Viewing Tube – Separated Eyepieces Dimensions Eyecups Dimensions Installing Objectives Installing the Substage Condenser Leica DM750 User Manual...
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Page 5: Safety Regulations
Safety Regulations Leica DM750 User Manual Safety Regulations... -
Page 6: Safety Concept
Safety Concept The individual modules of the Leica DM micros- Before installing, operating or using the instru- copy series include an interactive CD-ROM with ments, read the user manuals listed above. In all relevant user manuals in several languages. particular, please observe all safety instructions. -
Page 7: Symbols Used In This Instruction Manual
Instructions for disposing of the Failure to comply can cause the following: instrument, accessory components, Hazards to personnel and consumables. Functional disturbances or damaged instruments Leica DM750 User Manual Safety Regulations... -
Page 8: Important Notes
Important Notes Description User manual Accessories from third-party suppliers The Leica DM750 microscope meets today's This user manual includes important instruc- You can combine individual system articles with state of the art of technology. Nevertheless, tions related to operating safety, maintenance articles from external suppliers. -
Page 9: Instructions On Use
If using the devices outdoors, protect The Leica DM750 microscope can be used in them from dust and moisture. Never use electri- clean rooms without any problems. - Page 10 Once the product has reached the end of its When installing Leica products into third-party shipping or transporting individual modules. service life, please contact Leica Service or Sales products, the manufacturer of the complete about disposal. system or its dealer is responsible for follow-...
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Page 11: Health Risks And Dangers Of Use
Dangers during use Workplaces with microscopes facilitate Leica microscopy series are intended to reduce The Leica DM750 microscope may only be and improve the viewing task, but they the exertion of the user to a minimum. connected to a grounded socket. -
Page 12: Information For The Person Responsible For The Instrument
Information for the Person Responsible for the Instrument Information for the person responsible for Do not use the Leica DM750 microscope Only original Leica replacement parts may the instrument unless it is in perfect condition. be used in servicing the product. -
Page 13: Care Instructions
Remove coarse debris with a moistened disposable cloth. Optical surfaces should be cleaned with a Protect the Leica DM750 microscope from lint-free cloth, lens tissue, or cotton swab oil and grease. Never grease or oil mechani- Acetone, xylene or nitro-containing thin- moistened with a commercially available cal parts or sliding surfaces. -
Page 14: Accessories, Maintenance And Repair
Accessories, Maintenance and Repair Accessories Maintenance Repairs and service work Only the following accessories may be used The Leica DM750 microscope is basically Only original Leica Microsystems spare with the Leica DM750 microscope: maintenance-free. To ensure that it always parts may be used. -
Page 15: Electrical Data And Ambient Conditions
In order to maintain this condition and to ensure safe operation, Unplug the instrument before changing any fuses. The Leica the user must follow the instructions and warnings contained in DM750 has two fuses, which are located behind the power cord this instruction manual. receptacle. ... -
Page 16: The Leica Dm750
The Leica DM750 Leica DM750 User Manual The Leica DM750... -
Page 17: Introduction
Introduction Thank you for purchasing the Leica DM750 Compound Microscope from Leica Micro- systems. This model’s exclusive design features and full range of accessories make it a truly versatile, high quality-instrument. The instrument is to be used only as described. -
Page 18: Unpacking
These items are delivered in separate packages. Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise. Leica DM750 User Manual The Leica DM750... -
Page 19: Ready
Ready! Leica DM750 User Manual Ready! -
Page 20: Substage Illumination
Substage Illumination Substage illumination Type 1: Standard illumination Type 2: Koehler illumination The Leica DM750 is available with two Adjustable condenser centering with provided Adjustable condenser centering with thumb- types of Substage Illumination. Identify tool. screws and adjustable Koehler field diaphragm. -
Page 21: Attaching The Viewing Tubes
Identify which viewing tube you have: using the Allen key provided. Allen key 2. Set the dovetail into the tripod mount and Type 1: Leica EZ viewing tube with integrated tighten the setscrew carefully (without eyepieces using excessive force). This precisely posi-... -
Page 22: Leica Ez Viewing Tube - Integrated Eyepieces
Leica EZ Viewing Tube – Integrated Eyepieces 1. In order to use the captive wingscrew, 2. Reinstall the Leica EZ viewing tube onto the remove the setscrew that was delivered stand. with the stand. Leica EZ viewing tube with integrated eyepieces ... - Page 23 Leica EZ Viewing Tube – Integrated Eyepieces (Continued) You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again. The eyepieces are integrated into the Leica EZ viewing tube and preset;...
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Page 24: Leica Standard Viewing Tube - Separated Eyepieces
Leica Standard Viewing Tube – Separated Eyepieces 1. Insert the eyepieces into the tubes. 2. Secure the eyepieces in the tubes by tight- ening the silver screws at the bottom of the tubes using a standard Phillips screwdriver (not included in the delivery package). -
Page 25: Eyecups
In this case, go to section "Operation" on page 30. If you purchased your Leica DM750 in the form of individual compo- nents rather than the standard configuration, please continue with the "Installing Objectives"... -
Page 26: Installing Objectives
As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification. Leica DM750 User Manual Ready! -
Page 27: Installing The Substage Condenser
1. Move the specimen stage upwards as far as 3. Unscrew the two setscrews (or wingscrews The Leica DM750 has an open substage it will go by using the coarse focusing knob for Koehler stands) on the condenser condenser... - Page 28 You will center the condenser more accu- thus roughly centered. rately when you get to the "Complete Condenser Centering" section on page 31. Leica DM750 User Manual Ready!
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Page 29: Set
Set! Leica DM750 User Manual Set! -
Page 30: Turning On The Microscope
Plug in and turn on the microscope Always use your microscope on a hard, The Leica DM750 has a 5 V/1.5 A USB power 1. Plug the power cable of the microscope stable surface. connector in the center of the cord wrap. This into a corresponding grounded socket. -
Page 31: Complete Condenser Centering
3. Look at the X through the eyepieces and center it in the field of view by tightening Centered the setscrews with the tool included in the delivery package (or, for a Leica DM750 with Koehler illumination, tighten the Make sure that the condenser is in the knurled screws). -
Page 32: Using The Condenser
Match the line of the rotating ring with the objective magnification in use. Leica DM750 User Manual Set! -
Page 33: Prepare To View A Specimen Slide
2. Using the X/Y-stage control, position the stage by sliding it under the slide grips. specimen slide such that a part of the speci- men is under the objective used. Slide grips hold the slide in place. Slide grips Leica DM750 User Manual Set! -
Page 34: Focusing
The stand of the Leica DM750 has been calibrated at the factory so that the focus can be found from this position within 1.5 rota- tions of the fine focus. -
Page 35: Viewing Tube Adjustment
Be sure you are wearing your circle. eyeglasses or contact lenses. If you have a Leica DM750 with Koehler field diaphragm, continue with the "Koehler If you have a Leica DM750 with stand- Configuration" section on page 37. - Page 36 If you use a DM750 with standard illumi- When doing so, do not change the nation, continue with the "Oil Immersion height of the specimen stage.
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Page 37: Koehler Configuration
Koehler Configuration If your Leica DM750 is fitted with a field diaphragm for Koehler Illu- 2. Focus the leaves of the field diaphragm using the condenser focusing mination, use the following procedure to assure condenser cente- knob on the left side of the stage mount. - Page 38 Koehler Configuration (Continued) 3. Turn the condenser centering wingscrews simultaneously to center 4. Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm. of the field of view. Leica DM750 User Manual Set!
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Page 39: Oil Immersion Technique
Oil Immersion Technique 1. Search for the area on the specimen slide 3. Add a drop of immersion oil from Leica 4. Rotate the oil immersion objective (the that you want to study. to the area of the specimen slide you are objective labeled "OIL") to the working... - Page 40 Then bring the oil objective into the final position, so that the oil drop is between the front lens of the objective and the specimen slide. Leica DM750 User Manual Set!
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Page 41: Time Delay Shutoff
Time Delay Shutoff The Leica DM750 is equipped with a time Changing the status of the time delay The LED Illumination will flash to indicate delay shutoff capability, which automati- shutoff the time delay shutoff status was changed. cally turns the illumination off after 2 hours of 1. - Page 42 Leica DM750 User Manual...
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Page 43: Ready! Set! Go
Ready! Set! Go! Now all you need to do is change objectives, set the condenser aperture (and field diaphragm if you have a DM750 Koehler Stand) appropriately for the objective magnification you are using, and enjoy the view! Leica DM750 User Manual... -
Page 44: Care Of The Microscope
Care of the Microscope Leica DM750 User Manual Care of the Microscope... -
Page 45: General Maintenance
The microscope should always be covered with the plastic dust cover (provided with the instrument) when it is not in use. Leica DM750 User Manual Care of the Microscope... - Page 46 Illumination tion objectives, the objective has a small The Leica DM750 uses LED illumination. concave front lens of fairly short radius or Therefore, no lamp changing is required for curvature. The surface of this front lens can the life of the microscope.
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Page 47: Troubleshooting
Troubleshooting Leica DM750 User Manual Troubleshooting... -
Page 48: Troubleshooting
Check the condenser centering. Open the aperture diaphragm completely. The image is not uniformly illuminated. The objective magnification is too weak. Use a higher magnification. Unwanted stray light. Clean the specimen and neighboring lens surfaces. Leica DM750 User Manual Troubleshooting... - Page 49 Then move the mechanical stage with coaxial drive all the tion decreases after working a long time. way to the right. Manually press the screw that holds the mechanical stage even farther to the right, as far as it will go. Leica DM750 User Manual Troubleshooting...
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Page 50: Dimensions
Dimensions Leica DM750 User Manual Dimensions... - Page 51 Leica DM750 User Manual Dimensions...
- Page 52 Leica DM750 User Manual Dimensions...
- Page 53 Leica DM750 User Manual Dimensions...
- Page 54 The Leica Microsystems Industry Division’s focus is to support customers’ pursuit Denmark ∙ Ballerup 4454 0101 4454 0111 of the highest quality end result. Leica Microsystems provide the best and most France ∙ Nanterre Cedex 811 000 664 1 56 05 23 23 innovative imaging systems to see, measure, and analyze the microstructures in Germany ∙...
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Questions and answers
1. what is the pixel size? (in microns) of Leica DM 750 M microscope?, if the pixel size changes for each magnification, what is the pixel size (in microns) for 100X, 50X, 20X, and 10X ?