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Problem
Liquid flow is
constricted or
insufficient in all the
chimneys of the
wash station.
Liquid is not flowing
through a subset of
the chimneys in the
wash station.
Waste is
accumulating in the
wash station.
Tubing creeps
through the pump
head instead of
being secured in
place.
The tubing is
crushed or damaged
in the pump head.
AssayMAP 96AM Wash Station Maintenance Guide
Potential cause
The tubing is kinked or
blocked, or the pump
head clamp is too tight
for the tube size.
The inline filter is
clogged.
The tubing diameter of
the fill line is too small.
The chimneys are
blocked or damaged.
The tubing is kinked or
blocked, or the pump
head clamp is too tight
for the tube size.
The tubing diameter is
too small for the
required flow.
The pump head clamp is
too loose for the tube
size.
The pump head clamp is
too tight for the tube
size.
1 96AM Wash Station Maintenance Guide
Solution
Ensure the tubing is not kinked or blocked.
Replace the tubing as necessary.
If necessary adjust the pump head clamp, see the
Agilent
Pump Module User
Replace the inline filter.
See
"Tubing connections for 96AM Wash Station"
on page
14.
Replace the tubing on the fill line using larger
diameter tubing.
See
"Tubing connections for 96AM Wash Station"
on page
14.
To resolve the problem chimneys:
1
Use a plastic Pasteur pipette to quickly
aspirate liquid through the chimneys.
2
Run the Pump Reagent task in Bravo
Diagnostics to pump liquid through the
chimneys at a maximum rate to dislodge any
clogs.
3
If the problem persists, replace the problematic
chimneys. See
"Replacing damaged chimneys"
on page 11
Ensure the tubing is not kinked or blocked.
Replace the tubing as necessary.
If necessary adjust the pump head clamp, see the
Agilent
Pump Module User
Replace the tubing on the empty line, using larger
diameter tubing for the section that routes through
the pump head (active section).
See
"Tubing connections for 96AM Wash Station"
on page
14.
Adjust the tubing clamp for the tube size. See the
Agilent
Pump Module User
Adjust the tubing clamp for the tube size. See the
Agilent
Pump Module User
Testing the 96AM Wash Station
Guide.
Guide.
Guide.
Guide.
17
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