Centering The Condenser - Nikon LABOPHOT2-POL Instructions Manual

N. OPERATIONS IN DETAIL
8. Centering the Condenser
(Condenser focus knob, condenser centering screw, Field
diaphragm control ring)
(Before focusing and centering the condenser, implement Microscopy
procedure from 1) to 15) and focus on the specimen with 10 x
objective. )
CD
Close the field diaphragm to its smallest size by manipulating the field
diaphragm control ring. Rotate the condenser focus knob to move
the condenser vertically so that a sharp image of the field diaphragm
is formed on the specimen surface (Fig.
14~rn).
Image of field diaphragm
Fig.14
(2) If the image decenters from the viewfield of the eyepiece, bring it
roughly to the center of the viewfield by means of the condenser
centering screws (Figs.
14~[Z]
and 15).
Q) Change to the 40 x objective. Focus on the specimen by turning the
fine focus knob and form an image of the field diaphragm on the
specimen surface by manipulating the condenser focus knob.
@ When the image decenters from the viewfield of the eyepiece, bring it
to the center of the viewfield by means of the condenser centering
screws. At this time, adjusting the field diaphragm image to be
slightly smaller than the viewfield of the eyepiece may be convenient
for centering (Fig.
14-~).
Fig.15
22