Nikon LABOPHOT2-POL Instructions Manual page 36

Polarizing microscope
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V. OPTIONAL ACCESSORIES
2) Preparation
(1) Centering the lamp
CD
Make certain that the optical-path change-over knob is pushed in to
the limit.
(2) Turn ON the power switch on the transformer and set the voltage to
6V.
Q) Fully open the aperture diaphragm.
@ Place the ND filter on the stage and focus on it using the 10 x
objective.
@ Remove the eyepiece from the sleeve(or put the Bertrand lens into the
optical path), and looking into the exit pupil of objective, move the
lamp housing back and forth to form a sharp image of the lamp
filament on the diffuser of the exit pupil.
@ Manipulate the lamp centering screws to center the filament image on
the exit pupil.
(2)
Orientation of polarizer
CD
Roughly focus on the N D filter on the stage using the 40
x objective.
(2) Set the analyzer scale to "0".
Q) Bring the Bertrand lens into the optical path. Looking into the exit
pupil of the objective, rotate the polarizer rotation ring to form the
dark cross Image on the exit pupil. (Fig. 29)
Note
I
Take care not to touch the polarizer rotation ring while
observing the specimen, or the orientation of the polarizer will
have problems.
If it is touched by mistake, readjust the orientation.
Dark cross image
Fig.29
3) Objectives
Use the CF M Plan Achromat P series objectives (Strain-free, 210/45).
For microscopy, refer to the descriptions for diascopic polarizing
microscopy.
34

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