Centering The Condenser Lens; Orientation Of The Dia-Polarizer - Nikon LABOPHOT-POL Instructions Manual

Polarizing microscope
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Fig.
10
Repeat the above procedure two or three
times, and the rotation center of the stage
coincides with the cross lines center.
Carry out centering for each objective.
Fig.
11
Eyepiece
viewfield stop
[.Im .. a ge of field
diaphragm
iT \,
,/
,
\\
\
.... (')\. i
\
\~~)
)
rn~-/
Eyepiece
viewfield stop
6. Orientation of the Dii-pmarizer
·1
1) Place the specimen on the stage, focus on
the specimen using objective 40
X.
2) Set the analyzer scale on the intermediate
tube "P" to
O.
3)
Insert the dia-polarizer
into the bottom
of the condenser as shown in Fig. 12.
A half
position
of the
displacement
Rotate
1800
.
Target
3)
Rotate the stage about
1800,
and the target
is displaced from the center of the cross
Iines. Move the objective using the center-
ing tools so that the center of the cross
Iines comes to one half position of the
displacement of the target. (Fig. 10)
Di'''OI''i''~~
~
Fig.
12
4)
Remove the
eyepiece from
the sleeve.
Observing
the
exit
pupil
of
objective
inside, rotate the dia-polarizer
so as to
form a dark cross image on the exit pupil
as shown in Fig. 13.
5. Centeringtbe Condenser Le~'
~
I
1) Close the field diaphragm in the micro-
scope base to its smallest size by means of
the field diaphragm control
ring. Rotate
the condenser focus knob to move the
condenser vertically
so that a sharp image
of the field diaphragm is formed on the
specimen surface.
2) Bring the field diaphragm
image to the
center of the field of view by means of
the condenser centering screws.
(Fig.
11-[I])
3) Change over to the objective 40
x,
and
adjust
the field
diaphragm
so that the
image of the diaphragm is about the same
as the eyepiece viewfield stop, as shown in
Fig. 11 - ~.
If not centered, use the con-
denser centering screws again.
Dark cross image
Fig.
13
9

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