Summary of Contents for Beckman Coulter Cytomics FC 500
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Cytomics FC 500 With CXP Software Getting Started PN 177652A (May 2003) Beckman Coulter, Inc. Fullerton, CA 92835pn...
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This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.
Contents Welcome, 1 Cytomics FC 500 with CXP Software, 2 Cytometer, 4 FC 500 Workstation, 6 Online Help System, 8 Documents, 10 Reagent System, 12 Installing CXP Software, 12 Logging On to the FC 500 Workstation, 13 Shutting Down the FC 500 Workstation, 14...
Welcome The FC 500 includes a state-of-the- This booklet introduces the art Workstation that improves: Cytomics FC 500 with CXP software. The FC 500 provides Usability automated flow cytometric analysis including DNA analysis, Workflow efficiency immunophenotyping, and cellular Results management subsetting.
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The FC 500 can easily adapt to your and a variety of analysis methods: laboratory's work flow. This system has three acquisition modes: AutoMATOR Batch Analysis MultiFile Analysis MCL Automatic Listmode Compensation. MCL Manual Single Tube.
Cytomics FC 500 With CXP Software Cytometer MAIN COMPONENTS Cytometer Sends data to the Workstation. The Cytometer includes: Analyzer that counts, measures and computes parameters. Power Supply that provides voltages, vacuum and pressure. MCL that positions the sample tubes in the carousel for sampling.
Cytometer The Cytometer counts, measures and computes parameters. These functions are performed by the Cytometer: Initialize the system. Run samples. Provide visual display on the Cytometer Indicator Panel. Transmit data to the Workstation. Shutdown the system. The Power Supply controls voltages required by the circuitry.
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The Cytometer Indicator Panel provides a display of several Cytometer activities: Forward scatter Ready icon Side scatter Sample flow in process Low, medium, high flow rate Fluorescent channels 1-5 Blue laser ON Waste full icon Red laser shutter open Sheath fluid low icon...
FC 500 Workstation The FC 500 Workstation receives information from the Cytometer, sets protocols and panels, analyzes results, then displays, stores and prints the results. The CXP software gives you immediate access to these features: Displays the Cytometer Control dialog box to adjust the cytometer settings and select parameters.
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Some additional analysis features are: Creates a Surface Plot and selects the Data Source and Format. Creates an Overlay Plot to compare single parameter histograms. Creates a Tomogram Plot and selects the Data Source and Format. Allows batch analysis of stored data using a single file or multifile protocol.
Online Help System The FC 500 Workstation has a comprehensive Help System that includes all the information you need to operate the system. INFORMATION INCLUDED Procedures for using the FC 500. Concepts to better understand the FC 500. Reference information, such as FC 500 performance specifications and characteristics.
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SPECIAL FEATURES INCLUDED Contents list to locate information in a book-like fashion. Index list for finding key information quickly. Search utility to locate specific information. Glossary list for definitions of terms and fields.
Documents Your system comes with this Getting Started booklet. The other documents are available in hard copy only by order. To order hard-copy documents, contact your Beckman Coulter Representative. SHIPPED WITH SYSTEM Operator Manuals CD-ROM Getting Started PN 177628 PN 177652...
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Note: All information included in the hard-copy documents is available in the CXP software online Help system. AVAILABLE BY ORDER Instructions For Use Reference PN 175571 PN 175570 Day-to-day operating In-depth information about: procedures: • What the instrument does • Startup •...
Reagent System These reagents are available for use with the Cytomics FC 500 with CXP Software: IsoFlow™ Sheath Fluid ® COULTER CLENZ cleaning agent Quality control materials are also available for use with the FC 500. They include: Flow-Check™ Fluorospheres Flow-Set™...
[Page 1 of 2] a. Highlight your operator ID. b. Enter your password. 6. Select a protocol, if needed. Note: In the event that you cannot access the FC 500 online Help System from your instrument, contact your Beckman Coulter Representative.
Shutting Down the FC 500 Workstation After you have performed the Daily Shutdown procedure: to put the Cytometer in the Idle mode. in the main windows to exit. to save or not save the current protocol. (FC OFF) to turn off the Cytometer.
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