Beckman Coulter Cytomics FC 500 Instructions For Use Manual
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Beckman Coulter Cytomics FC 500 Instructions For Use Manual

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See also: User Manual , Manual
Cytomics FC 500
With CXP Software
PN 624923BF (July 2016)
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instructions For Use

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Summary of Contents for Beckman Coulter Cytomics FC 500

  • Page 1 Cytomics FC 500 With CXP Software Instructions For Use PN 624923BF (July 2016) Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.

  • Page 2: Warnings And Precautions

    This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.

  • Page 3: Revision Status

    This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for your instrument.
  • Page 4 REVISION STATUS PN 624923BF...

  • Page 5: Table Of Contents

    CONTENTS WARNINGS AND PRECAUTIONS, ii REVISION STATUS, iii CONTENTS, v ILLUSTRATIONS, xi TABLES, xii INTRODUCTION, xiii USING YOUR Cytomics FC 500 MANUALS, xiii ABOUT THIS MANUAL, xiii CONVENTIONS, xiv To Choose A Command With The Keyboard, xv Dialog Box, xv...

  • Page 6: Contents

    CONTENTS Carousels, 1-13 Bar Code Labels, 1-13 Putting a Bar Code Label on a Sample Tube, 1-13 Putting Sample Tubes in a Carousel, 1-14 Putting a Carousel in the MCL, 1-14 Removing a Carousel from the MCL, 1-15 CXP SOFTWARE SHORTCUTS, 1-16 Keyboard Shortcuts, 1-17...
  • Page 7 CONTENTS How to Use Help, 1-39 Help Menu, 1-39 Help, 1-39 About... , 1-39 Online Support, 1-39 1.14 PERFORMANCE CHARACTERISTICS, 1-40 DAILY ROUTINE, 2-1 BEFORE YOU BEGIN, 2-1 DAILY STARTUP, 2-2 Check Waste and Reagent Levels, 2-2 Power the Computer and Cytometer ON, 2-3 Turning On Power, 2-3...
  • Page 8 CONTENTS Before Running the AutoSetup II Wizard, 3-8 APPLICATION DEFINITION WIZARD, 3-14 Before Running the Application Definition Wizard, 3-14 Setting Up QC Products, 3-18 Exporting Region Results To QC Database and Assigning QC Products, 3-20 Review QC Results, 3-21 RUNNING SAMPLES, 4-1 SAMPLE REQUIREMENTS, 4-1...
  • Page 9 CONTENTS AUTOMATED SOFTWARE FEATURES, 5-14 PARAMETERS, 5-14 AUXiliary Parameter, 5-14 When to Use the AUX Parameter, 5-14 TIME Parameter, 5-15 RATIO Parameter, 5-15 5.10 PLOT DISPLAY, 5-15 PRISM, 5-16 Regions, 5-17 Gating, 5-17 Data Storage, 5-17 Histogram Statistics, 5-18 Linear Region Statistics, 5-18 Log Region Statistics, 5-19...
  • Page 10 BAR CODE LABLES, B-6 Bar Code Label Optical Characteristics at 670 nm ±10%, B-6 MCL BAR CODE READER, B-6 BAR CODE DECODER, B-7 CHECKSUM ALGORITHM, B-7 REFERENCES, REFERENCES-1 TRADEMARKS, 1 Cytomics FC 500 with CXP Software Documentation, 2 PN 624923BF...

  • Page 11: Illustrations

    CONTENTS ILLUSTRATIONS Flow Cell (Hydrodynamic Focusing), 5-2 Laser Beam Shaping, 5-3 Optical System with Optional Red Solid-State Laser, 5-5 Optical System with Optional HeNe Laser, 5-5 Single Laser Filter Block Configuration, 5-6 Dual Laser Filter Block Configuration, 5-7 Second Laser Optional Filter Configuration, 5-8 Voltage Pulse Formation, Peak Signal, 5-11...

  • Page 12: Tables

    CONTENTS TABLES Applications for the Instrument, 1-2 Cleaning Schedule, 6-12 Error Messages, 6-17 Software Messages, 6-23 Bar Code Symbologies, B-5 Code-Related Specifications, B-6 PN 624923BF...

  • Page 13: Introduction

    INTRODUCTION This introductory section contains the following topics: USING YOUR Cytomics FC 500 MANUALS ABOUT THIS MANUAL CONVENTIONS GRAPHICS CYBERSECURITY CONTACT US USING YOUR Cytomics FC 500 MANUALS The manuals listed below are available as PDF files in the FC 500 CXP software. Printed versions of these manuals are also available by order.
  • Page 14 INTRODUCTION CONVENTIONS HOW TO... BAR CODE SPECIFICATIONS INDEX Provides page numbers for indexed information. CONVENTIONS This manual uses the following conventions: Throughout this manual your FC 500 is also referred to as the system or instrument. indicates a software option, such as Bold font Cytometer Italics font indicates screen text displayed on the instrument, such as...
  • Page 15 If you have any questions, contact our Customer Support Center. Worldwide, find us via our website at www.beckmancoulter.com/customersupport/support. In the USA and Canada, call us at 1-800-369-0333. Outside of the USA and Canada, contact your local Beckman Coulter Representative. PN 624923BF...
  • Page 16 INTRODUCTION CONTACT US PN 624923BF...
  • Page 17 PERFORMANCE CHARACTERISTICS INTENDED USE The Cytomics FC 500 is a system intended for use as an in vitro diagnostic device for the qualitative and quantitative measurement of biological and physical properties of cells and other particles. These properties are measured when the cells pass through one or two laser beams in single-file.

  • Page 18: Applications For The Instrument

    SYSTEM OVERVIEW INTENDED USE Table 1.1 Applications for the Instrument Sample Preparation (refer to the reagent's Applications Cell Types package insert) Measurements Parameters Cell surface Whole blood ImmunoPrep reagents with Cell size and Forward and side antigens the Q-PREP Workstation, the granularity scatter (Log/Linear) Buffy coats...
  • Page 19 SYSTEM OVERVIEW CYTOMETER CONTROLS AND INDICATORS CYTOMETER CONTROLS AND INDICATORS FC 500 Flow Cytometry System 5571026B Mouse Cytometer USB box HeNe laser option Computer Keyboard Power Supply Monitor PN 624923BF...

  • Page 20: Indicators,

    SYSTEM OVERVIEW CYTOMETER CONTROLS AND INDICATORS Cytometer Indicator Panel Cytometer Ready and Laser On Indicators Blue means the Argon laser is on. Red means the red laser shutter is open. Green means Cytometer is on and in the Ready state. PN 624923BF...
  • Page 21 SYSTEM OVERVIEW CYTOMETER CONTROLS AND INDICATORS Sample Flow Rate Indicators Low Sample Flow Medium Sample Flow High Sample Flow Signal Amplitude Indicators Note: When both log and linear parameters are selected for the same sensor, the amplitude display defaults to the log signal. Red means overrange FL5 or Aux White indicates Aux source and...

  • Page 22: Loader),

    SYSTEM OVERVIEW CYTOMETER CONTROLS AND INDICATORS Level Sense and Flow Indicators Sheath Flow Waste Full Sheath Low MCL (Multi-tube Carousel Loader) PN 624923BF...

  • Page 23: Power Supply,

    SYSTEM OVERVIEW CYTOMETER CONTROLS AND INDICATORS Workstation MultiSync LCD 1700M+ Power Supply Your instrument has one of these configurations. Follow the procedures in the documentation that applies to your Power Supply configuration. Universal Power Supply Voltage-Specific Power Supply FILTER WATER TRAP TRAP SYSTEM POPWER...

  • Page 24: Start Button,

    SYSTEM OVERVIEW MICROSOFT® WINDOWS® DESKTOP ® ® MICROSOFT WINDOWS DESKTOP Windows 2000 Administrator Password Be sure to maintain your Windows 2000 Administrator password in a secure location. If you lose or forget the Windows 2000 Administrator password, you must reimage your hard drive, causing the loss of all data.

  • Page 25: Recycle Bin,

    SYSTEM OVERVIEW MICROSOFT® WINDOWS® DESKTOP Other standard Windows functions are also displayed and these are briefly described below: Programs: displays a list of programs that are installed on the computer that you can start. Documents: lists the most recently opened documents. Settings: displays the user-configurable system components, including the Control Panel.

  • Page 26: My Computer,

    SYSTEM OVERVIEW MICROSOFT® WINDOWS® DESKTOP My Computer The My Computer icon on the Windows Desktop allows access to the contents of your computer (installed drives, the Control Panel, Printers and other icons as appropriate to the way the particular computer system was configured). If you wish to see the contents of any drive or folder, double click the mouse on its icon.

  • Page 27: Using A Mouse,

    SYSTEM OVERVIEW MICROSOFT® WINDOWS® DESKTOP Using a Mouse The Mouse, a handheld input device, controls the movement of a pointer on the computer screen. As the mouse is moved, the pointer moves on the screen. When you position the pointer over an object on the screen, you can click the mouse button to do different tasks. You can click the mouse button to select files, double click to open and work in files, or click and drag to move files.

  • Page 28: Color Resolution,

    SYSTEM OVERVIEW MICROSOFT® WINDOWS® DESKTOP Color Resolution For the best results, you need to reset your computer to a color resolution of at least 1024 x 768 pixels and High Color (16 bit). Close down any applications that you may have running, including any that have been minimized on the Tool Bar.

  • Page 29: Mcl Carousels

    SYSTEM OVERVIEW LEARNING THE BASIC OPERATING TECHNIQUES LEARNING THE BASIC OPERATING TECHNIQUES Before reading the other chapters in this manual: Read about the Cytometer Control screens in the Reference manual. Read this chapter to become experienced with using the MCL. Practice the basic techniques until you feel comfortable using them.

  • Page 30: Putting Sample Tubes In A Carousel

    SYSTEM OVERVIEW LEARNING THE BASIC OPERATING TECHNIQUES Putting Sample Tubes in a Carousel r The orientation of a tube with a bar code label does not matter. The MCL rotates the tube to find the bar code label. r Do not skip tube positions within a panel. The FC 500 flow cytometer does not skip a protocol in a panel when a carousel tube position is empty.

  • Page 31: Removing A Carousel From The Mcl

    SYSTEM OVERVIEW LEARNING THE BASIC OPERATING TECHNIQUES Close the MCL cover. Removing a Carousel from the MCL Open the MCL cover. 1-15 PN 624923BF...

  • Page 32: Cxp Software Shortcuts

    SYSTEM OVERVIEW CXP SOFTWARE SHORTCUTS Remove the carousel. Close the MCL cover. CXP SOFTWARE SHORTCUTS CXP software uses several standard Windows techniques for providing shortcuts to functions. Keyboard Shortcuts Drag And Drop Toolbar Buttons 1-16 PN 624923BF...

  • Page 33: Keyboard Shortcuts

    SYSTEM OVERVIEW CXP SOFTWARE SHORTCUTS Keyboard Shortcuts Ý+X Ý Where means hold down and press Ý+ Copy Ý+ Paste Ý+ Ý+ Undo Ý+ Redo Ý+ Workspace Preferences Ý+ Tile Special Ý+ Print Ý+ New Protocol Ý+ Save Protocol Ý+ Open Listmode File Ý+ Format Plot Ý+...

  • Page 34: Drag And Drop

    SYSTEM OVERVIEW CXP SOFTWARE SHORTCUTS Drag And Drop Dragging and dropping is a shortcut method of opening, moving and deleting files or other objects. Within CXP software the Drag and Drop technique can be used for several functions, with Ý some operations can be held down to modify the default behavior.

  • Page 35: Toolbar Buttons

    SYSTEM OVERVIEW CREATING PROTOCOLS Toolbar Buttons Toolbars form an essential part of the CXP software. The application entered and the mode you are operating at the time determines whether a particular Toolbar display is available. Toolbars can be moved using the Drag and Drop method with the mouse around the CXP software desktop or customized to suit the preferences of each user.
  • Page 36 SYSTEM OVERVIEW CREATING PROTOCOLS If the parameters have changed, Enter the parameter name and on the Cytometer Control screen. Create the Plots required for acquisition. If analysis is to be performed during acquisition, create the required Regions and Gates, and also select the required statistics from the option.
  • Page 37 SYSTEM OVERVIEW CREATING PROTOCOLS Set up the Worklist and run samples. Setup Mode on the Cytometer Control Acquisition Setup Tab and adjust the instrument settings on the Cytometer Control Detector Tab. When satisfied with the data, , deselect Setup Mode and continue acquisition.

  • Page 38: Creating Regions

    SYSTEM OVERVIEW CREATING REGIONS CREATING REGIONS Create Polygonal Regions Create Rectangular Regions Create Quadrant Regions Create Linear Regions Create Polygonal Regions See also: Interactive Polygonal Region Editing in the Reference manual. Highlight a dual parameter plot (click on the title bar). A Polygonal Region cursor is displayed on screen.
  • Page 39 SYSTEM OVERVIEW CREATING REGIONS Close the Region by returning the cursor to the starting box and clicking to conclude Region creation. The Region Name appears on the plot. Dot Plot Data Source to display percentages with the Region name. 1-23 PN 624923BF...

  • Page 40: Create Rectangular Regions

    SYSTEM OVERVIEW CREATING REGIONS Create Rectangular Regions See also: Interactive Rectangular Region Editing in the Reference manual. Highlight a dual parameter plot. A Rectangular Region cursor appears in the highlighted plot. Position the cursor to the point where you want the Region to begin. Click and release the mouse button.

  • Page 41: Create Quadrant Regions

    SYSTEM OVERVIEW CREATING REGIONS Create Quadrant Regions Highlight a dual parameter plot. A crosshair cursor is displayed in the current window. Position the cursor on the plot where you want the intersection point of the region and to show the quadrant lines (cross hair). Move the regions to the required position, if needed, then to set this position.

  • Page 42: Repositioning A Quadrant Region

    SYSTEM OVERVIEW CREATING REGIONS Repositioning a Quadrant Region Mouse over the Quadrant region lines to get the cursor. Reposition the region. + hold and drag to move the vertical divider. + hold and drag to move the horizontal divider. + hold and drag to move the Quadrant region.

  • Page 43: Create Linear Regions

    SYSTEM OVERVIEW CREATING REGIONS Create Linear Regions This option requires two points to be set giving an upper and lower channel boundary for every Region. Highlight a single parameter histogram. A Linear Region cursor appears in the current window. Position the cursor on the chosen plot to the point where you want the Region to begin. Click, two vertical lines with a horizontal bar appears.

  • Page 44: Create Multiple Linear Regions

    SYSTEM OVERVIEW CREATING REGIONS Create Multiple Linear Regions Highlight a single parameter histogram and A Parallel cursor appears in the current window. Position the cursor on the chosen plot to the point where you want the Region to begin. Click, two vertical lines with a horizontal bar is displayed. A stop box appears in the right hand top corner or the window.

  • Page 45: Creating Gates

    SYSTEM OVERVIEW CREATING GATES CREATING GATES Create AutoGate Note: If a stop count is used on a plot that contains an AutoGate region, the stop count is not exact. in the plot you want to create an AutoGate on. on the population to AutoGate. 1-29 PN 624923BF...
  • Page 46 SYSTEM OVERVIEW CREATING GATES To specify the AutoGate Sensitivity and Travel, the region. Click the right mouse button on the region and Region Properties Specify the AutoGating Sensitivity Contour Travel, if necessary, Right mouse click on the plot you want to assign the AutoGate to and select Format to display the Plot Properties dialog.

  • Page 47: Convert A Polygonal Region To An Autogate Region

    SYSTEM OVERVIEW CREATING GATES Convert a Polygonal Region to an AutoGate Region region to make it active. Click the right mouse button on the region and Region Properties either Elliptical Contour in Polygonal Region Properties screen. Automatic Gate Creation This option allows you to create a new gate automatically when a new Region is drawn.

  • Page 48: Creating Flowpages

    SYSTEM OVERVIEW CREATING FLOWPAGES CREATING FLOWPAGES FlowPAGE Example Below is a typical example of a FlowPAGE. This is a screen view only. A full printed page outputs at a much higher resolution. See also: FlowPAGE Menu in the Using CXP Software chapter in the Reference manual.

  • Page 49: Delete Flowpages

    SYSTEM OVERVIEW CREATING PANELS Delete FlowPAGES in the upper left corner of the FlowPAGE window to be deleted and Close Note: FlowPAGES in a locked protocol can not be inserted, deleted or edited. 1.10 CREATING PANELS The Panel Wizard allows you to create a Panel for use in the Acquisition Manager. Remarque : The default protocol (Ý...
  • Page 50 SYSTEM OVERVIEW CREATING PANELS if you want a Patient Panel Export the results of this panel to the Report Generator Report generated when you run this panel. See Panel Report in the Reference manual. Choose your Protocol Settings. Use Plots and Gates from Previous Test Use Plots and Gates from Protocol Use Instrument Settings Choose your Region Settings.

  • Page 51: Use Plots And Gates From Previous Test

    SYSTEM OVERVIEW CREATING PANELS Use Plots and Gates from Previous Test Allows you to use Gates and Plots from a previous Test but is not active for the first Test within a Panel. This option is not available if is chosen. Use plots and gates from protocol Use Plots and Gates from Protocol Allows you to select Plots and Gates from a specific Protocol, chosen from the drop down list...

  • Page 52: 1.11 Creating Worklists

    QC Applications or AutoSetup protocols are run. Modifications to a multi-tube panel may impact any associated panel templates or Worklists. Beckman Coulter recommends that all templates and Worklists containing a panel that has been modified be validated before use.

  • Page 53: Worklist Panel

    SYSTEM OVERVIEW CREATING WORKLISTS Worklist Panel Select which panel you wish to open After all the information has been added, you can use Drag And Drop to change the order. You can use this option to add another Panel to an existing Worklist. Worklist Test to insert a single Row at the end of the current Panel.

  • Page 54: 1.12 Verifying A Worklist

    Preferences. Also, the default protocol name will default to "setup.def" in any associated panel template and have no regions. 1.13 USING THE ONLINE HELP SYSTEM Your Cytomics FC 500 system provides an online Help system that allows you to search for information on specific system-related topics through the options.

  • Page 55: How To Use Help

    Beckman Coulter Web Site If you have an Internet connection and click the mouse on http://www.beckmancoulter.com, you can access the Beckman Coulter Web Site through your Web Browser program. You can then access the latest information concerning the up-to-date software releases and other items of interest.

  • Page 56: 1.14 Performance Characteristics

    SYSTEM OVERVIEW PERFORMANCE CHARACTERISTICS 1.14 PERFORMANCE CHARACTERISTICS Refer to the package insert of the Quality Control Materials listed in the Reference manual for the performance characteristics for the preparation method you are using. 1-40 PN 624923BF...

  • Page 57: Daily Routine

    DAILY ROUTINE BEFORE YOU BEGIN This chapter explains the daily startup procedures. Before doing these procedures: Read the OPERATION PRINCIPLES chapter. Using your system is easier if you have a general understanding of how it works. Read the SYSTEM OVERVIEW chapter.

  • Page 58: Daily Startup

    DAILY ROUTINE DAILY STARTUP DAILY STARTUP Perform the following steps to start up the system. If you have set up CYTOMETER AUTO STARTUP and the Cytometer is running, skip ahead to Check the Power Supply. Check Waste and Reagent Levels Empty the waste container and verify tubing is connected to the cap.

  • Page 59: Power The Computer And Cytometer On

    DAILY ROUTINE DAILY STARTUP Check the cleaning agent fill level b fill the cleaning agent container necessary. Power the Computer and Cytometer ON Turning On Power Turn on the system at the computer. PN 624923BF...

  • Page 60: Logging Onto Windows Software

    DAILY ROUTINE DAILY STARTUP Logging Onto Windows Software When the Log on to Windows screen appears, Check that the field User name displays Administrator or your Windows log-in user name. If not, type in your user name. Note: When first installing the software, the field must User name...

  • Page 61: Logging Onto Cxp Software

    DAILY ROUTINE DAILY STARTUP Logging Onto CXP Software to start the software and power up the Cytometer. Allow about 40 minutes to warm up the system before performing QC or running samples. At the CXP Cytometer Startup Wizard [Page 1 of 2] screen: Highlight your User ID.

  • Page 62: Check The Power Supply

    DAILY ROUTINE DAILY STARTUP Check the Power Supply Open the Power Supply door and check the WATER TRAP , AIR FILTER, and FILTER VACuum FILTER. Contact your Beckman Coulter WATER TRAP TRAP Representative if: FILTER The TRAP is >1/3 full.

  • Page 63: Supply,

    DAILY ROUTINE DAILY STARTUP Adjust the pressure to 30 ±2 psi. b To decrease, turn to the left. c To increase, turn to the right. Push in on the knob to lock it into place. Check the VACuum TRAP . If it is >1/4 full of fluid CLEAN THE FILTER...

  • Page 64: Additional Start Up Checks

    DAILY ROUTINE DAILY SHUTDOWN Additional Start Up Checks Check that the MCL vortex function mixes samples by running a blank sample. Refer to the manuals that came with your Printer to: Perform Printer diagnostics. Check that there is an adequate paper supply in the Printer. Check ink cartridges if you have a color Printer and replace if necessary.

  • Page 65: Power The Computer And Cytometer Off

    DAILY ROUTINE DAILY SHUTDOWN Power the Computer and Cytometer OFF to put the Cytometer in the Idle mode. in all open windows. to turn off the Cytometer. shutdown the Workstation. PN 624923BF...
  • Page 66 DAILY ROUTINE DAILY SHUTDOWN When the Shut Down Windows screen appears: Ensure Shut down or Standby appears below What do you want the computer to The Cytometer automatically turns off. Turn off the monitor and Printer separately. 2-10 PN 624923BF...

  • Page 67: After Instrument Shut Down

    DAILY ROUTINE DAILY SHUTDOWN After Instrument Shut Down WARNING Risk of chemical injury from bleach. To avoid contact with the bleach, use barrier protection, including protective eyewear, gloves, and suitable laboratory attire. Refer to the Safety Data Sheet for details about chemical exposure before using the chemical. Wipe down all exposed surfaces (and the 40 tube holder if used) with 10% bleach solution and then 70% ethanol.

  • Page 68: Extended Shutdown

    DAILY ROUTINE EXTENDED SHUTDOWN EXTENDED SHUTDOWN If you intend to leave the instrument in the shutdown state for an extended amount of time: Remove the cleaning agent and sheath fluid containers. Rinse the inside of both containers with water. 2-12 PN 624923BF...
  • Page 69 DAILY ROUTINE EXTENDED SHUTDOWN Replace the cleaning agent and internal sheath fluid containers. Fill both containers with water instead of reagent. Perform the Routine Cleaning Procedure. Use tap water in tubes 1-4 instead of bleach in tube 1 and IsoFlow sheath fluid in tubes 2-4.

  • Page 70: Cytometer Auto Startup

    DAILY ROUTINE CYTOMETER AUTO STARTUP CYTOMETER AUTO STARTUP You can set up the system to automatically Startup or Shutdown the Cytometer. The computer must be on with Windows running to allow Auto Startup to run. Using Windows Explorer, double click on C:\CXP\Tasks.exe.
  • Page 71 DAILY ROUTINE CYTOMETER AUTO STARTUP to schedule when you want the Startup or Shutdown task to occur. and enter your settings. to specify additional settings. If you want to set up automatic Cytometer shutdown, and perform steps through 5. 2-15 PN 624923BF...
  • Page 72 DAILY ROUTINE CYTOMETER AUTO STARTUP 2-16 PN 624923BF...

  • Page 73: Quality Control

    QUALITY CONTROL INTRODUCTION Perform the following quality control checks to ensure that your system is working accurately and precisely. The protocols needed for these quality control (QC) procedures are included with your system Software. The package inserts for your Quality Control Materials have instructions for establishing your laboratory's normal ranges for daily use.

  • Page 74: Discriminator Value

    QUALITY CONTROL INSTRUMENT SETUP Discriminator Value A threshold value is applied to the selected parameter signal to control the amount of background that is measured. Setting the discriminator too low could include a higher amount of debris while a setting that is too high might eliminate the desired events. See Discriminators in the Reference manual for information on selecting and modifying discriminators.

  • Page 75: Qc Processes

    QUALITY CONTROL QC PROCESSES QC PROCESSES The chart below shows which QC materials are needed for each QC process. QC Process QC Material Used Verify fluidics and laser Flow-Check Fluorospheres (Flow-Check 675 Fluorospheres, Flow-Check alignment 770 Fluorospheres). Verify HPCV versus expected value. Export results the QC Database and review the QC...

  • Page 76: Daily Qc

    QUALITY CONTROL DAILY QC DAILY QC Daily QC consists of: Preparing AutoSetup Samples Running the AutoSetup Scheduler Running the AutoSetup II Wizard IMPORTANT Risk of erroneous results if the Cytometer has been idle for an extended period of time or you have just performed Daily Startup.
  • Page 77 QUALITY CONTROL DAILY QC Prepare the appropriate Flow-Set fluorospheres for the applications you need to run. The Flow-Set fluorospheres tube is used to set the detector gains and voltages to the required level. The fluorospheres you use to perform standardization depend upon which application you are using, as shown in the chart below.

  • Page 78: Autosetup Scheduler

    QUALITY CONTROL AUTOSETUP SCHEDULER AUTOSETUP SCHEDULER Use the AutoSetup Scheduler to select the applications that you need to run on the instrument in a given day or "shift". AutoSetup Scheduler groups the selected applications and provides the carousel load report to facilitate setting up and loading samples for daily QC. Tools tt AutoSetup Scheduler.
  • Page 79 QUALITY CONTROL AUTOSETUP SCHEDULER to print the Carousel Load Report. Note: If absolute counts are required, and use the Acquisition Manager to enter the CAL Factor. Place tubes in the carousel in the order shown on the Carousel Load Report. on the Carousel Load Report screen to run AutoSetup II.

  • Page 80: Autosetup Ii Wizard

    QUALITY CONTROL AUTOSETUP II WIZARD AUTOSETUP II WIZARD AutoSetup II simplifies and automates application setup and QC monitoring for multiple applications simultaneously. You can perform a QC setup for all of your multicolor applications that you run during a single day or shift at the same time for common instrument optical configurations using the Application Definition Wizard and the...
  • Page 81 QUALITY CONTROL AUTOSETUP II WIZARD on the Carousel Load Report screen in the AutoSetup Scheduler. ALIGNMENT APPLICATIONS After data acquisition for the Alignment Application starts, view all histograms to ensure peaks fall within regions. Setup Mode on the Cytometer Control Acquisition Setup Tab.
  • Page 82 QUALITY CONTROL AUTOSETUP II WIZARD When acquisition of the Alignment stops, check the HPCV status displayed on the AutoSetup II Wizard dialog. If HPCVs pass: on the AutoSetup II Wizard. to continue with another scheduled QC application. If QC Failed is displayed, one or more HPCVs are not within the upper limits identified in the protocol’...
  • Page 83 QUALITY CONTROL AUTOSETUP II WIZARD The Wizard adjusts Volts and Gains to place the Flow-Set fluorospheres within the regions. The Wizard dialog box displays a list of the parameters remaining to be adjusted. If the Wizard cannot adjust peaks to the regions within 60 seconds, AutoSetup aborts and the parameters that failed are listed.
  • Page 84 QUALITY CONTROL AUTOSETUP II WIZARD Verification samples are run to ensure the calculated coefficients are satisfactory for the samples and antibodies being used in your tests. The QuickCOMP slider bars are automatically enabled and the AutoSETUP Wizard is displaying Adjust Compensation setting if necessary then click <Acquire>...
  • Page 85 QUALITY CONTROL AUTOSETUP II WIZARD Check the printed verification tube results to ensure instrument settings are correct and Note: Due to differences between individual instruments, settings files should not be transferred from one instrument to another. If you attempt to use settings files from another instrument, the software displays Incorrect Cytometer Serial Number.

  • Page 86: Wizard

    QUALITY CONTROL APPLICATION DEFINITION WIZARD APPLICATION DEFINITION WIZARD Use the Application Definition Wizard to define your applications and save the definitions for use by the AutoSetup Scheduler. The application definition captures the instrument setup, lasers, parameters, fluorochromes, target channels, verification and alignment requirements of a particular application.
  • Page 87 QUALITY CONTROL APPLICATION DEFINITION WIZARD and select the protocol to use as the base protocol for the application. The Protocol Summary displays a description of the parameters and laser setup for the selected protocol. the filter block to use for the application and Note: An alignment application may not have a User defined Filter Block...
  • Page 88 QUALITY CONTROL APPLICATION DEFINITION WIZARD the dyes you use for each fluorescence detector and Select a QC product and enter a target channel for Forward Scatter, Side Scatter and each fluorescence detector. Note: If you are running a 5 color 2 laser application, enter Fluorescence and Forward Scatter target channels for the Flow-Set 675 and Flow-Set 770...
  • Page 89 QUALITY CONTROL APPLICATION DEFINITION WIZARD Enter names for the application and the instrument settings file and Choose to have the wizard create a verification protocol or add a saved verification protocol or panel. Use the buttons to add, remove and reorder multiple verification protocols.

  • Page 90: Setting Up Qc Products

    QUALITY CONTROL APPLICATION DEFINITION WIZARD Setting Up QC Products to display the Report Generator toolbar. on the Report Generator toolbar to display the Edit Products screen. Create or update a QC product: to create a new QC product. Select a product from the QC Product list to modify an existing product.
  • Page 91 QUALITY CONTROL APPLICATION DEFINITION WIZARD To save a new QC product: to save the new QC product information. when prompted to confirm the creation of the new QC product. again. when prompted to confirm changes. To save changes to an existing product: to save the updates to the QC product information.

  • Page 92: Exporting Region Results To Qc Database And Assigning Qc Products

    QUALITY CONTROL APPLICATION DEFINITION WIZARD Exporting Region Results To QC Database and Assigning QC Products on a region to make it active. Right mouse click on the region and Region Properties Ensure that Region Statistics exported for Quality Control next to QC Product and select the QC Product to assign to the region.

  • Page 93: Review Qc Results

    QUALITY CONTROL APPLICATION DEFINITION WIZARD Review QC Results to display the Report Generator toolbar. on the Report Generator toolbar to display the QC Levey Jennings screen. next to Application to select an application. Template tt Open Template. the desired template and Repeat steps to view other QC data plots.
  • Page 94 QUALITY CONTROL APPLICATION DEFINITION WIZARD 3-22 PN 624923BF...

  • Page 95: Running Samples

    RUNNING SAMPLES SAMPLE REQUIREMENTS At least 0.5 mL of prepared sample is needed. It must be in a 12- x 75-mm test tube. Samples analyzed on the instrument must be in a single-cell suspension. Typically, cells are prepared before they are analyzed. The method used to prepare a specimen depends on the sample type and the assay desired.
  • Page 96 RUNNING SAMPLES BEFORE RUNNING SAMPLES IMPORTANT Risk of erroneous results if the Cytometer has been idle for an extended period of time or you have just performed Daily Startup. To ensure correct results, perform a prime after: r Daily Startup. r The Cytometer has been idle for an extended period of time.

  • Page 97: Running Samples - Mcl Automatic Mode

    RUNNING SAMPLES RUNNING SAMPLES - MCL AUTOMATIC MODE RUNNING SAMPLES - MCL AUTOMATIC MODE an example of MCL Automatic Mode sample processing. Verifying A Worklist Always verify carousel and tube location fields before starting acquisition and ensure that the Acquisition Manager information accurately reflects the tubes in the carousel being run. The order of the tubes in the carousel must match the order of the tubes in the Worklist.
  • Page 98 RUNNING SAMPLES RUNNING SAMPLES - MCL AUTOMATIC MODE WARNING Risk of injury. Do not open the MCL cover while the MCL is moving. To avoid injury, wait until the MCL stops moving before opening the MCL cover. Open the MCL cover and place the carousel on the MCL.
  • Page 99 RUNNING SAMPLES RUNNING SAMPLES - MCL AUTOMATIC MODE Enter the carousel ID number in the Acquisition Manager. IMPORTANT Risk of erroneous results if the sample becomes diluted. When operating the Cytometer, if the MCL cover is opened, an audible alarm sounds and a warning message is displayed. If acquisition completes while the MCL cover is opened, do not use the sample for subsequent processing.

  • Page 100: Running Samples - Mcl Manual Mode

    RUNNING SAMPLES RUNNING SAMPLES - MCL MANUAL MODE RUNNING SAMPLES - MCL MANUAL MODE MCL Manual Mode operates the same as MCL Automatic Mode except the MCL stops after processing each tube to allow you to edit the Sample ID. CAUTION Possible flow cell damage.
  • Page 101 RUNNING SAMPLES RUNNING SAMPLES - MCL MANUAL MODE Close the MCL cover. To stop after processing each tube and edit the Sample ID, Workspace Edit Sample IDs Preferences - Acquisition Options Use the Acquisition Manager Toolbar Drag And Drop to select the Worklist, Panel or Protocol you want to use.
  • Page 102 RUNNING SAMPLES RUNNING SAMPLES - MCL MANUAL MODE to place the Cytometer in MCL Manual Mode. IMPORTANT Risk of erroneous results if the sample becomes diluted. When operating the Cytometer, if the MCL cover is opened, an audible alarm sounds and a warning message is displayed. If acquisition completes while the MCL cover is opened, do not use the sample for subsequent processing.
  • Page 103 RUNNING SAMPLES RUNNING SAMPLES - MCL MANUAL MODE If you enabled Edit Sample IDs Workspace Preferences - Acquisition Options edit the sample ID after the tube finishes processing and Note: After the first tube in a panel, Sample ID 1 cannot be changed. Enter the location of the next tube to process and Repeat steps...

  • Page 104: Running Samples - Single Tube Mode

    RUNNING SAMPLES RUNNING SAMPLES - SINGLE TUBE MODE RUNNING SAMPLES - SINGLE TUBE MODE The Single Tube Mode operates by introducing tubes individually into the MCL through the MCL Tube Access door. S an example of Single Tube Mode sample processing. Note: When operating in the Single Tube Mode, the Prime function is not available when the system is between samples, even though the toolbar is active.
  • Page 105 RUNNING SAMPLES RUNNING SAMPLES - SINGLE TUBE MODE to place the Cytometer in Single Tube Mode. The MCL moves to put carousel position 10 under the Tube Access door. WARNING Risk of injury. Do not open the MCL cover while the MCL is moving. To avoid injury, wait until the MCL stops moving before opening the MCL cover.
  • Page 106 RUNNING SAMPLES RUNNING SAMPLES - SINGLE TUBE MODE Observe the Events/Sec counter on the Status Bar to monitor data acquisition. Note: While running a Worklist, if the Tube ID in the Worklist does not match the bar code on the tube, a system message appears. After you acknowledge the message, the mismatched tube is aborted and the processing stops for the rest of the carousel.

  • Page 107: Data Review

    RUNNING SAMPLES DATA REVIEW DATA REVIEW IMPORTANT Risk of reporting incorrect results. Data displays used to arrive at the test result for light scatter patterns, antibody staining profiles, as well as all gates and boundaries should be reviewed by a laboratory professional when interpreting the data.
  • Page 108 RUNNING SAMPLES DATA REVIEW 4-14 PN 624923BF...

  • Page 109: Operation Principles

    OPERATION PRINCIPLES WHAT THIS CHAPTER EXPLAINS This chapter explains how the Cytometer measures scattered light and fluorescence as cells pass through the laser beam. The illustrations in this chapter are not exact representations of the inside of the Cytometer. They are for explanatory purposes only. SAMPLE FLOW CAUTION Possible flow cell damage.

  • Page 110: Focusing),

    OPERATION PRINCIPLES SAMPLE FLOW Figure 5.1 Flow Cell (Hydrodynamic Focusing) To waste To waste Sensing area has rectangular channel Sample stream enters here Sample stream Sheath stream enters here Sheath stream Direction of laser beam Cells PN 624923BF...

  • Page 111: Laser Beam Shaping

    OPERATION PRINCIPLES LASER BEAM SHAPING LASER BEAM SHAPING Before the laser beam reaches the sample stream, cross-cylindrical lenses focus the beam (see Figure 5.2). Focusing keeps the beam perpendicular to the sample stream flow while making the beam small enough to illuminate only one cell at a time. The first lens controls the width of the beam;...

  • Page 112: Cell Illumination

    OPERATION PRINCIPLES CELL ILLUMINATION CELL ILLUMINATION As cells in the sample stream go through the sensing area of the flow cell, the elliptical laser beam illuminates them. The cells scatter the laser light and emit fluorescent light from fluorescent dyes attached to them. Forward Scatter The amount of laser light scattered at narrow angles to the axis of the laser beam is called forward scatter (FS).

  • Page 113: Optical System With Optional Red Solid-State

    OPERATION PRINCIPLES LIGHT COLLECTION, SEPARATION AND MEASUREMENT Figure 5.3 Optical System with Optional Red Solid-State Laser Argon (488) laser Field stop SS sensor FS sensor Solid-state (635) laser (optional) Flow cell Figure 5.4 Optical System with Optional HeNe Laser Argon (488) laser Field stop SS sensor FS sensor...

  • Page 114: Side Scatter And Fluorescent Light Collection

    OPERATION PRINCIPLES LIGHT COLLECTION, SEPARATION AND MEASUREMENT Side Scatter and Fluorescent Light Collection In order for the sensors to measure SS and FL, the light must be collected and the SS and fluorescent light must be separated. The pickup lens/spatial filter assembly collects SS and FL from only the sensing area of the flow cell, and collimates it.
  • Page 115 OPERATION PRINCIPLES LIGHT COLLECTION, SEPARATION AND MEASUREMENT Figure 5.6 Dual Laser Filter Block Configuration 488 DLP 675 BP 525 BP 500 LP 710 DLP 530 SP 600 DLP 755 BP Flow cell 615 DSP 575 BP 620 SP 550 DLP PN 624923BF...

  • Page 116: Second Laser Optional Filter

    OPERATION PRINCIPLES LIGHT COLLECTION, SEPARATION AND MEASUREMENT An additional 655 LP filter has been added to the second laser filter configuration as shown in the illustration below: Figure 5.7 Second Laser Optional Filter Configuration 488 DLP 655 LP 550 DLP 500 LP 675 BP 525 BP...

  • Page 117: Fluorescent Light

    OPERATION PRINCIPLES LIGHT COLLECTION, SEPARATION AND MEASUREMENT Fluorescent Light The light the 488 DLP filter transmits goes to a 500 nm long pass filter. This filter blocks any remaining laser light, transmitting only fluorescent light. The remaining optical filters separate the light for the five FL sensors. Note: This fluorescent light collection description applies to the Dual Laser Filter Block Configuration only.

  • Page 118: Signal Processing

    OPERATION PRINCIPLES SIGNAL PROCESSING SIGNAL PROCESSING Voltage Pulse Signals The Cytometer has seven sensors, each generating a voltage pulse signal as each cell passes through the laser beam. A voltage pulse signal is proportional to the intensity of light the sensor received.

  • Page 119: Voltage Pulse Formation, Peak

    OPERATION PRINCIPLES SIGNAL PROCESSING Figure 5.8 Voltage Pulse Formation, Peak Signal Laser beam Time Cell Pulse height Volts 5-11 PN 624923BF...

  • Page 120: Integral Signal

    OPERATION PRINCIPLES SIGNAL PROCESSING Integral Signal Because the total fluorescence in all three cells is the same, but the distribution is different, the pulse can be integrated to produce an integral signal (see Figure 5.9). The height of the integral pulse is proportional to the total fluorescence and is obtained when the cell exits the laser beam.

  • Page 121: Signals Generated

    OPERATION PRINCIPLES PROTOCOLS Signals Generated The instrument sensors can generate these signals (integral unless stated otherwise; LOG stands for logarithmic): FS, FS LOG, FS PEAK SS, SS LOG, SS PEAK FL1, FL1 LOG, FL1 PEAK FL2, FL2 LOG, FL2 PEAK FL3, FL3 LOG, FL3 PEAK FL4, FL4 LOG, FL4 PEAK FL5, FL5 LOG, (FL5 uses the AUX signal pathway).

  • Page 122: Special Panels

    OPERATION PRINCIPLES AUTOMATED SOFTWARE FEATURES Special Panels The cleanse panel utilizes two cleanse protocols (‘cleanse bleach.PRO’ and ‘cleanse di water.PRO) that you should use at the end of the working day or shift on your FC 500. AUTOMATED SOFTWARE FEATURES CXP software contains the following automated software features.

  • Page 123: Time Parameter

    OPERATION PRINCIPLES PLOT DISPLAY TIME Parameter The TIME parameter is the amount of time, in seconds, the instrument acquires data. It is displayed on the plot axis in 1-second resolution. The axis labels vary, depending on plot resolution and stop time (duration). The minimum stop time is 10 seconds, the maximum stop time (maximum duration) is 99,999 seconds and the default stop time is 300 seconds (5 minutes).

  • Page 124: Prism

    OPERATION PRINCIPLES PLOT DISPLAY PRISM Prism is used to analyze multicolor immunofluorescence samples. With multicolor immunofluorescence a cell is either positive or negative for each of two, three, four or five cell surface markers. A particular combination is called a phenotype. Prism allows you to display percentages on all phenotypic populations in a single plot.

  • Page 125: Regions

    OPERATION PRINCIPLES PLOT DISPLAY Regions To analyze data or gate plots, you must first create and assign regions to these tasks. You can create five different types of regions (see Regions Introduction). The region types are: Linear Rectangular Quadrant Polygonal Elliptical Contour On Overlayed histograms only...

  • Page 126: Histogram Statistics

    OPERATION PRINCIPLES PLOT DISPLAY Histogram Statistics Linear Region Statistics For linear signals, statistics for histogram regions are calculated as follows: Number of events in region × total ------------------------------------------------------------------------------------------------------- - Total number of events in the gated display Number of cells in region ×...

  • Page 127: Log Region Statistics

    OPERATION PRINCIPLES PLOT DISPLAY Log Region Statistics The method of geometric calculations of Means depends on your Advanced Statistics Configuration. Log-Log Mean Method Weighting each channel according to its face value as given by the log scale performs this calculation of Mean. Example: When using a four-decade uncalibrated logarithmic display reporting output as Channels, you might give channel zero (the lowest channel) a value 10...

  • Page 128: Lin-Log Mean Method

    OPERATION PRINCIPLES PLOT DISPLAY Lin-Log Mean Method This method of Mean calculation is worked out by performing a simple arithmetic mean channel calculation on raw data according to its channel value in the range 0 to 1023 (for 1024 channel data). The Mean value can then be reported as channels (in the range 0 to 1023) or converted to the Relative Channel value.

  • Page 129: Troubleshooting

    The Cytometer and the MCL bar code reader each contain a laser. The Cytometer can also include an optional second laser. Beckman Coulter's design and manufacture of the instrument complies with the requirements governing the use and application of a laser as specified in regulatory documents issued by the: U.S.

  • Page 130: Laser Labels On The Sensing Compartment

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.1 Laser Labels on the Sensing Compartment Cover VISIBLE AND/OR INVISIBLE LASER RADIATION LASER RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM AVOID EYE OR SKIN CLASS 3B LASER PRODUCT EXPOSURE TO DIRECT OR SCATTERED RADIATION 7272009A PN 624923BF...

  • Page 131: Laser Labels In The Optical Area, Front

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.2 Laser Labels in the Optical Area, Front View AVOID EXPOSURE AVOID EXPOSURE VISIBLE AND/OR INVISIBLE VISIBLE AND/OR LASER RADIATION WHEN OPEN INVISIBLE LASER RADIATION IS AVOID EYE OR SKIN EXPOSURE TO DIRECT OR EMITTED FROM SCATTERED RADIATION THIS APERTURE.

  • Page 132: Laser Labels In The Optical Area, Interior

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.3 Laser Labels in the Optical Area, Interior View VISIBLE AND/OR INVISIBLE LASER RADIATION WHEN OPEN AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION CAUTION Laser radiation when open and interlock defeated. DO NOT STARE INTO BEAM. AVOID EXPOSURE VISIBLE AND/OR INVISIBLE LASER RADIATION IS EMITTED...

  • Page 133: Laser Labels On The Argon Laser

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.4 Laser Labels on the Argon Laser Head LASER RADIATION LASER RADIATION-AVOID IS EMITTED FROM DIRECT EXPOSURE TO BEAM THIS APERTURE ARGON/.50 WATT C.W. AVOID EXPOSURE CLASS IIIb LASER PRODUCT LASER RADIATION WHEN OPEN AVOID DIRECT EXPOSURE TO BEAM 7272007A Figure 6.5 Laser Labels for Optional Red Solid-State Laser PART NUMBER/REVISION...

  • Page 134: Location Of The Optional Red Solid-State Laser In The Instrument, Rear

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.6 Location of the Optional Red Solid-State Laser in the Instrument, Rear View TH IS L ASER PR OD UC T CO NF ORMS TO TH E PR OV IS IO NS OF 21 CF R. SU BC HA PTER J, SE CT IO NS 10 40 .1 AN D 040.

  • Page 135: Location Of The Optional Red Helium-Neon Laser In The Instrument, Rear

    AND 1040.11. VISIBLE AND/OR INVISIBLE Manufactured: LASER RADIATION WHEN OPEN DATE: AVOID EYE OR SKIN Beckman Coulter, Inc. EXPOSURE TO DIRECT OR 250 S. Kraemer Blvd., SCATTERED RADIATION Brea, CA 92821 THIS LASER PRODUCT CONFORMS TO THE PROVISIONS OF 21 CFR.

  • Page 136: Laser Labels On The Mcl Probe Housing

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.10 Laser Labels on the MCL Probe Housing Cover AVOID EXPOSURE VISIBLE AND/OR INVISIBLE LASER RADIATION IS EMITTED FROM THIS APERTURE. CLASS 1 LASER PRODUCT CAUTION CAUTION Laser radiation when open LASER LIGHT DO NOT STARE INTO BEAM and interlock defeated.

  • Page 137: Laser Labels On The Mcl Bar Code

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Figure 6.11 Laser Labels on the MCL Bar Code Reader LASER LIGHT IS EMITTED AVOID EXPOSURE FROM THIS APERTURE CAUTION VORSICHT LASER LIGHT DO NOT STARE INTO BEAM 670 nm LASER DIODE 1.0 MILLIWATT MAX CLASS II LASER PRODUCT CAUTION LASER LIGHT WHEN OPEN-DO NOT STARE INTO BEAM.

  • Page 138: Warning Labels On Ups

    TROUBLESHOOTING PRECAUTIONS/HAZARDS Warning Labels on UPS label located near the circuit breakers on the Universal Power Supply instructs the user to refer to product documentation before resetting the circuit breakers. label located next to the power switch on the Universal Power Supply instructs the user to refer to product documentation before powering up the instrument.

  • Page 139: Disposal Of Electrical Instrumentation

    European Union. For products under the requirement of WEEE directive, please contact your dealer or local Beckman Coulter office for the proper decontamination information and take back program which will facilitate the proper collection, treatment, recovery, recycling, and safe disposal of device.

  • Page 140: Maintenance Schedules

    TROUBLESHOOTING MAINTENANCE SCHEDULES MAINTENANCE SCHEDULES Cleaning Schedule Table 6.1 for the cleaning schedule. Table 6.1 Cleaning Schedule Component Daily Weekly Monthly Every 60 Days As Needed CLEAN THE AIR FILTERS CLEAN THE CLEANING AGENT CONTAINER CLEAN THE MCL SAMPLE HEAD AND THE SAMPLE PROBE CLEAN THE SAMPLING SYSTEM CLEAN THE SHEATH FLUID...

  • Page 141: Error Messages

    TROUBLESHOOTING ERROR MESSAGES ERROR MESSAGES Table 6.2 for a list of all of the error messages. Display Locations Error messages can appear at the bottom of the CXP software screen, in the Cytometer Status Messages screen, and in the cytometer.log file. CXP Software Screen At the bottom of the CXP software screen, the last error message appears.

  • Page 142: Cytometer.log File

    TROUBLESHOOTING ERROR MESSAGES Cytometer.log File In the cytometer.log file, all messages appear from the last 30 days unless they were manually cleared. Refer to ERROR MESSAGE CYTOMETER.LOG FILE for details. See Figure 6.15. Figure 6.15 Example of Error Messages in Cytometer.log File 6-14 PN 624923BF...

  • Page 143: Error Message Cytometer.log File

    TROUBLESHOOTING ERROR MESSAGE CYTOMETER.LOG FILE ERROR MESSAGE CYTOMETER.LOG FILE The Cytometer error messages are located in the cytometer.log file. Note: Error messages over 30 days old are automatically removed from the cytometer.log file and placed into the cytometerarchive.log file. How to Access the Cytometer.log File From CXP Software Press Ý+L.

  • Page 144: Other Functions Available

    TROUBLESHOOTING ERROR MESSAGE CYTOMETER.LOG FILE Other Functions Available Here are some of the more often used functions available from the cytometer.log file pull down menus: , and File Edit Format Help Print To print the cytometer.log file: File Print Change Font To change the font: Format Font...

  • Page 145: Error Messages Table,

    These are the error messages produced by the Cytometer. Contact your Beckman Coulter Representative if: The recommended action does not solve the problem. You need help.
  • Page 146 (sample tube) in the MCL area. If no obstruction is found, contact your Beckman Coulter Representative. MCL CPU Error MCL hardware error. Contact your Beckman Coulter Representative. MCL Door Open Error MCL cover is open while the MCL is Close the MCL cover.
  • Page 147 Table 6.2 Error Messages (Continued) Message Cause Action MCL Receive Timeout Error An MCL communication error. Contact your Beckman Coulter Representative. MCL Request Error Communication error between the 1. Power the instrument off then on. Cytometer and the MCL. 2. If problem continues, contact your Beckman Coulter Representative.
  • Page 148 SHEATH FLUID CONTAINER. fluid for about 5 minutes of sample analysis. If the sheath fluid container is full, Contact your Beckman Coulter the sheath fluid sensor failed. Representative. Sheath Pressure Error The sheath fluid pressure is outside 1. Check sheath fluid and cleaning agent the system’s operating range.
  • Page 149 TROUBLESHOOTING ERROR MESSAGES TABLE Table 6.2 Error Messages (Continued) Message Cause Action Temperature Sensor Failure Temperature sensor failure. Contact your Beckman Coulter Representative. Temperature Warning Temperature inside Cytometer is 1. Clean your air filters. See CLEAN THE above 50°C. FILTERS.
  • Page 150 There is enough empty volume in Empty the waste container. See EMPTY the waste container for about 5 THE WASTE CONTAINER. minutes of sample analysis. If the waste container is not full, the Contact your Beckman Coulter waste sensor failed. Representative. 6-22 PN 624923BF...
  • Page 151 Table 6.3 lists the software messages, with their cause and what to do about them. These are messages produced by the Software. Contact your Beckman Coulter Representative if: The recommended action does not solve the problem. You need help. Table 6.3 Software Messages...
  • Page 152 Table 6.3 Software Messages (Continued) Message Cause Action Error - Failed to set user logoff in System call to exivd failed during log Contact your local Beckman Coulter exivd. off. Representative. The profiles have been modified The modified user profiles cannot be Redo work.
  • Page 153 Table 6.3 Software Messages (Continued) Message Cause Action Could not write the directories Error loading the directory paths file. Contact your local Beckman Coulter locations to disk. This will not stop Representative. CXP running, but it is highly recommended that it is corrected as areas such as Clinical Panels and Quality Control rely on this file.
  • Page 154 TROUBLESHOOTING SOFTWARE MESSAGES Table 6.3 Software Messages (Continued) Message Cause Action Error reading archive file. A system level error occurred when None, message is informational. reading the printer file. Error reading file. A system level error occurred when None, message is informational. reading the printer file.
  • Page 155 Correct the cytosettings in the protocol. protocol. caused an error. Warning: One or more error(s) After approving the AutoSetup there Contact your local Beckman Coulter occurred while saving the settings was a system error, previously Representative. file(s). reported, moving the settings file(s).
  • Page 156 The carousel will be aborted. Bad data pointers! System would not lock memory to Contact your local Beckman Coulter enable painting or printing of Representative. histogram. Failed to create empty document. System message to say document Contact your local Beckman Coulter for new view could not be created.
  • Page 157 TROUBLESHOOTING SOFTWARE MESSAGES Table 6.3 Software Messages (Continued) Message Cause Action The panel file <XYZ > does not exist. The panel file requested in the EWL Ensure that the panel file is present in cannot be found in any of the search correct location locations.

  • Page 158: Level Sense Indicators,

    TROUBLESHOOTING LEVEL SENSE INDICATORS 6.10 LEVEL SENSE INDICATORS Sheath Low When the Sheath Low indicator (see Figure 6.16) appears: During sample analysis, you have 5 minutes to finish analyzing the current sample. You cannot analyze samples or use the instrument until the sheath fluid container is filled.

  • Page 159: Viewed,

    TROUBLESHOOTING CYTOMETER CONTROL WINDOW CANNOT BE VIEWED 6.11 CYTOMETER CONTROL WINDOW CANNOT BE VIEWED If the Cytometer Control window cannot be viewed (it is hidden behind the Windows Taskbar at the bottom of the screen), the Auto hide feature is currently selected. Change the Windows settings to deselect the Auto hide feature and keep the Cytometer Control window in view.
  • Page 160 TROUBLESHOOTING CYTOMETER CONTROL WINDOW CANNOT BE VIEWED 6-32 PN 624923BF...
  • Page 161 HOW TO... SOFTWARE PROCEDURES For Online help, click on the links below to go to the detailed procedure. These procedures are in the System Overview chapter in the Instructions For Use Manual. CREATING PROTOCOLS CREATING REGIONS CREATING GATES CREATING FLOWPAGES CREATING PANELS CREATING WORKLISTS These procedures are in the Quality Control chapter in the Instructions For Use Manual.
  • Page 162 HOW TO... HARDWARE PROCEDURES PN 624923BF...

  • Page 163: B Bar Code Specifications,

    BAR CODE SPECIFICATIONS BAR CODE SAMPLE IDENTIFICATION Bar code symbols are a highly accurate and efficient procedure for identifying and processing laboratory samples. Beckman Coulter instruments use four bar code symbologies (types) to identify specimens: Code 128 ® Code 39 Codabar Interleaved 2-of-5.

  • Page 164: B.3 Bar Code Label Specifications,

    BAR CODE SPECIFICATIONS CORRECT PLACEMENT OF THE BAR CODE LABEL CORRECT PLACEMENT OF THE BAR CODE LABEL The bar code label must be placed a minimum of 25.4 mm (1.0 in.) from the bottom of the tube. Refer to Figure B.2.

  • Page 165: Label Size And Thickness,

    BAR CODE SPECIFICATIONS BAR CODE LABEL SPECIFICATIONS Label Size and Thickness The length of the label must be less than 44.45 mm (1.75 in.). The label includes the bar code symbol and a minimum quiet zone of 3.5 mm (0.14 in.) at each end of the symbol. Refer to Figure B.3.

  • Page 166: B.4 Bar Code Error Rate,

    Select the fixed length option, if available, because it is more accurate than the variable length option. To keep label and printing flaws to a minimum, use a narrow element of more than 0.25 mm (0.010 in.). Beckman Coulter recommends the use of: Code 128 Checksum for all other symbologies Fixed length code symbols Narrow bar sizes of 0.25 mm (0.010 in.) minimum.

  • Page 167: Bar Code Symbologies

    BAR CODE SPECIFICATIONS BAR CODE SYMBOLOGIES BAR CODE SYMBOLOGIES Beckman Coulter instruments use four bar code symbologies for specimen identification, see Table B.1.Within the given specifications, the MCL reader and the optional handheld bar code reader automatically distinguish the following bar codes: Table B.1 Bar Code Symbologies...

  • Page 168: Code-Related Specifications

    BAR CODE SPECIFICATIONS BAR CODE LABLES BAR CODE LABLES A bar code consists of black lines (bars) and white lines (spaces), which are called elements. There are narrow elements (NE) and wide elements (WE). The bar code symbology determines their arrangement. IMPORTANT Sample misidentification can occur from the use of incorrect, poor quality, damaged, dirty or improperly placed bar code labels.

  • Page 169: B.8 Bar Code Decoder,

    Use checksums to provide protection against occasional misread errors caused by problems such as damaged or misapplied labels. If you must use bar codes without checksums, Beckman Coulter recommends that you verify each bar code reading to assure correct patient identification.
  • Page 170 BAR CODE SPECIFICATIONS CHECKSUM ALGORITHM PN 624923BF...
  • Page 171 REFERENCES American Identification Manufacturer’ s group (AIM), Uniform Symbology Specifications Code 39, Interleaved 2 of 5, Codabar, and International Symbology Specifications Code 128. ANSI/AIM BC1, BC2, BC3, BC4, 1995. http://www.aimusa.org American National Standards Institute (ANSI) Bar Code Print Quality Guidelines. X3. 182-1990 (R2000).
  • Page 172 REFERENCES REFERENCES-2 PN 624923BF...
  • Page 173 Numerics schedule, 6-12 system pressure, 3D acceleration air filter setting needed on video card, 1-12 daily check, 3dfx voodoo based video cards when to contact service, setting needed for 3D acceleration, 1-12 alignment 3-of-9 verify, bar-code, amplification 488 DL filter description, 5-12 used to separate SS,...

  • Page 174: Bar Code Label

    auto shutdown Code 128, how to set up, 2-14 Code 39, auto startup NW7, how to set up, 2-14 USD-3, AutoGate region USD-4, convert from a polygonal region, 1-31 USD-6, creating, 1-29 bar code decoder stop count on plot, 1-29 description, automated software bar code labels...

  • Page 175: Bar Code Label

    Taskbar, Codabar See also icons, Start button bar code, Codabar bar code checksum algorithm, Code 128 calculating bar code, compensation coefficients, 3-11 Code 39 can not retrieve offset parameter resolution, 6-28 bar code, can not retrieve parameter resolution, 6-27 Code 39 bar code can not retrieve x parameter resolution., 6-28 checksum algorithm,...
  • Page 176 whole blood, auto shutdown, 2-14 See also individual QC material names auto startup, 2-14 conventions controls and indicators, used in the manual, front view, correct settings indicator panels, verify, laser labels, 6-2, 6-3, 6-4, could not build the in-memory directories list, 6-24 ready and laser on indicators, could not write the directories locations to disk,...

  • Page 177: Error Messages

    Data Stream Warning, 6-17 error creating directory name, 6-24 delete error invalid file, 6-25 Recycle Bin, error messages Desktop CXP software screen, 6-13 Windows, Cytometer Status Window, 6-13 Desktop Menu Bar cytometer.log, description, 6-15 illustration, cytometerarchive.log file, 6-16 dialog box description, 6-13 description,...
  • Page 178 500 LP, function, verify, air, location, fluorescence air, when to clean, 6-12 intensity determines peak pulse height, 5-10 configuration, four FL sensors, fluorescent light (FL) daily check at startup, cell illumination, dichroic, function, collection, long pass (LP), function, indicators, spatial, function, when to use, example, fluorochrome See fluorescent light (FL)

  • Page 179: Sheath Low

    ground samples, user, if static charge exists, Immuno-Brite fluorospheres QC process used for, See also controls Immuno-Trol Cells hazards QC process used for, radiation exposure, See also controls Helium Neon laser Immuno-Trol Low Cells See HeNe laser QC process used for, help See also controls about,...
  • Page 180 lin-log mean method, 5-20 labels listmode bar code, specifications, description, 5-17 CDRH-required, log on disposal of electrical instrumentation, instructions, warning, 6-11 Windows screen, laser, warning, log zero gives minus infinity !!, 6-28 RoHS caution, 6-11 log-log RoHS environmental, 6-11 mean method, 5-19 universal power supply, warning, 6-10...
  • Page 181 MCL EPROM Error, 6-18 creating, 1-28 MCL Error, 6-18 My Computer MCL Interlock Error, 6-18 Windows, 1-10 MCL manual mode See manual mode MCL Parallel Recv Error, 6-18 navigation pane MCL Parallel Send Error, 6-18 illustration, 1-39 MCL Probe Up/Down error, 6-18 location, 1-39...
  • Page 182 photo sensors See sensors package inserts pickup lens/spacial filter assembly contain fluorospheres instructions, 3-4, function, reagent, use to prepare samples, 4-3, 4-6, 4-10 plots use to establish expected ranges, drag and drop function, 1-18 use to establish normal ranges, PRISM, 5-16 use to establish target channels, 3-8, 3-14...
  • Page 183 drag and drop functions, 1-18 protocols radiation hazards, AS, description, 5-13 ranges, laboratory’s cleanse bleach.pro, 5-14 establish expected ranges, cleanse di water.pro, 5-14 when to re-establish, Cleanse, description, 5-13 RATIO parameter creating, 1-19 description, 5-15 definition, 5-13 formula, 5-15 description, 5-13 reagents drag and drop function,...

  • Page 184: Mode,

    set color monitor, 1-12 See also carousel, sample and MCL resource explorer sample flow drag and drop functions, 1-18 description, results rate indicators, review QC results, 3-21 sample ID RoHS can edit after each tube in manual mode, caution label, 6-11 can edit after each tube in single tube mode, 4-10...

  • Page 185: Software Messages

    low indicator, 1-6, 6-30 signing on to CXP software, sheath fluid container software messages when to clean, 6-12 list of, 6-23 sheath fluid flow indicator table location, See also error messages Sheath Level Error, 6-20 solid-state laser Sheath Level Warning, 6-20 illustration, sheath low indicator...
  • Page 186 running fluorospheres, the file selected describes a panel that has a different sample cycle messages, 4-5, 4-8, 4-11 number of tubes to the panel file, 6-26 startup cycle messages, the panel file xyz does not exist, 6-29 stop count the profiles have been modified outside of this effect on AutoGate region, 1-29 application,...
  • Page 187 bar-code, fluidics systems, USD-3 HPCV versus expected value, bar code, verifying a worklist: update, 1-38, USD-4 video cards bar code, setting needed for 3D acceleration, 1-12 USD-6 virus protection program bar code, shutdown computer, virus scan instrument settings, 1-35 not while running CXP , instrument settings from external file, 1-35 voltage pulse signals...
  • Page 188 Help, My Computer, 1-10 password security, Programs, Recycle Bin, Run, Search, Settings, Shut Down, Start button, Taskbar, wizard AutoSetup, running, panel See panel wizard worklist external files (EWL), 1-37 how to select, 4-4, 4-7, 4-10 panel, 1-37 test, 1-37 worklist, verifying, 1-38, Workstation front view, INDEX-16...
  • Page 189 TRADEMARKS The Beckman Coulter stylized logo and COULTER are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders. PN 624923BF...
  • Page 190 Cytomics FC 500 with CXP Software Documentation Reference Use and Function • Installation • Specifications • Log Sheets • Using PN 175570 CXP Software • Glossary • Index Special Procedures Cleaning Procedures • Replace/Adjust Procedures • Index PN 175572 Instructions For Use System Overview •...

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