Pipetting Speed Control; Guidelines For Good Pipetting - VWR International VWR 10 Instruction Manual

• Beep: makes pipette produce a beep sound while selected tasks are executed
To transfer a protocol to your VWR Electronic Pipette, click the "Transfer custom protocols to
pipette" button and follow the instructions on the screen.
The CUSTOM mode is available in pipettes from firmware version cf01.00.03
For more information on VWR Protocol builder software, see page 34.
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PIPETTING SPEED CONTROL

VWR Electronic Pipette is set by default on speed 6 (maximum speed). You may need to change
the speed of aspiration or dispensing depending on your application. You can change aspiration
speed and dispensing speed independently of one other: from very slow to very fast (speed 1 –
speed 6). Your speed selection is memorized for each pipetting mode until you change it again.
• Press the Mode-button to access system menu, where you can find the different pipette modes,
the speed menu and configuration menu.
• Select SPEED by turning the Push-button, to confirm your selection click on the Push-button.
• Set ASPIRATION SPEED: aspiration speed is blinking, set the speed by turning the Push-button
and click to confirm your selection.
• Set DISPENSING SPEED: dispensing speed is blinking, set the speed by turning the Push-button
and click to confirm your selection.
• After selecting the speed you go automatically back to pipetting mode.
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GUIDELINES FOR GOOD PIPETTING

Aspirate and dispense:
1/ Fit a suitable tip
2/ Pre-rinse the tip. Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it
back into the same vessel (or to waste). Subsequent volumes that you pipette will have levels of
accuracy and precision within specifications. Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a film of liquid on the inside the wall of the tip; pre-rinsing the tip
minimizes any errors that may be related to this phenomenon.
3/ Hold the pipette vertically and immerse the tip in the liquid . Press the push-button to aspirate the
set volume of liquid. Wait a few seconds then withdraw the pipette-tip from the liquid. You may wipe
any droplets away from the outside of the tip using a medical wipe, however if you do so take care
to avoid touching the tip's orifice.
4/ Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
Press the Push-button.
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