Introduction Introduction 1.1 Symbols Signal words Signal word Meaning DANGER! This combination of symbol and signal word indicates an imme- diate dangerous situation that will result in death or serious injury if it is not avoided. WARNING! This combination of symbol and signal word indicates a possible dangerous situation that can result in death or serious injury...
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Introduction Ä Chapter 1.1 ‘Sym- Cross reference within the docu- Cross references are indicated as follows: ment bols’ on page 5 Symbols on the device Warnings/symbols on the device which are no longer recognizable should be immediately replaced by the operating company. The images shown in the following depict the posi- tions of the warnings and symbols affixed to the device.
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Introduction Fig. 2: Warning stickers on the right outer side Attention, general danger point Nonobservance of this warning can lead to material damage and personal injury. Before using the device, make sure you read the operating instruc- tions and observe the safety information. Symbol for separate collection of electric and electronic devices Symbol according to directive 2002/96/EC (WEEE).
Andreas Hettich GmbH & Co. KG. Authorized skilled personnel with special instruction Skilled personnel are able to perform the work assigned to them...
Introduction Personnel have participated in special instruction by the manufac- turer of the device or personnel authorized by the manufacturer and, through this, are authorized to perform the corresponding task. Personnel have completely read and understood this documenta- tion. Service technician A service technician is someone who, based on his professional training and advanced practical knowledge, provides expert sup- port (installation, commissioning, maintenance, etc.) for our cus-...
If original replacement parts, consumable material approved or sold by the manufacturer and original accessories are not used, any warranty or liability claims against the company Andreas Hettich GmbH & Co. KG are void. 1.6 Scope of delivery 1 x device with mounted rotor...
Introduction 1.7 Return shipments If the device or its accessories are sent to the manufacturer, this/ these must be decontaminated and cleaned before shipping in order to protect personnel, the environment and material. Please contact the Service department of the manufacturer for the return shipment.
Safety information Safety information The device and its associated components and assemblies meet the currently valid safety stand- ards individually and as a whole and meet the CE directives of the European Union. The device is safe if used as intended and observing the descriptions and information given in this documentation.
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Safety information Danger due to insufficient mainte- nance or maintenance not per- DANGER! formed in a timely manner Danger due to insufficient maintenance or maintenance not performed in a timely manner! There is a risk of collision inside the device due to insufficient maintenance or maintenance not per- formed in a timely manner.
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Safety information Risk of injury due to the device falling out of the packaging WARNING! Risk of injury due to parts falling out of the packaging! When parts fall out of the transport packaging, there is a risk of crushing injuries to extremities. –...
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Safety information Danger posed by selection of unsuitable parameters for dual cen- WARNING! trifugation Danger posed by selection of unsuitable parameters for dual centrifugation! The maximum permissible speed (RPM) for Zen- triMix applications depends on the sample compo- sition and sample container. Every ZentriMix sample container can only be used up to a certain rotational speed depending on the design and application;...
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Safety information Danger due to hazardous sub- stances in the sample DANGER! Danger due to hazardous substances in the sample No materials or material mixtures may be pro- cessed in a way which poses a risk of fire or explo- sion.
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Safety information Danger due to the penetration of water or other liquids DANGER! Danger due to the penetration of water or other liquids! When water penetrates into the device, there is a danger of a short circuit and the user suffering an electric shock.
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Safety information Mechanical emergency release WARNING! There is a risk of injury if the mechanical emer- gency release of the device is actuated during the program run. If the mechanical emergency release of the lid is actuated during a program run, the program is aborted and the rotor runs down unbraked.
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Safety information ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
Device description Device description Control panel Further information about the control panel can be Ä Chapter 6 ‘Oper- found in the Operation chapter ation’ on page 31. Fig. 4: Operating elements, front side Centrifuging chamber Rotor cavity Fig. 5: Device interior Device plug (power supply) Device switch [On/Off] Fig.
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Device description RS232 interface Fig. 7: Rear side ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
Transport and storage Transport and storage Dimensions and weight with trans- port packaging ca.620 ca.925 Fig. 8: Transport packaging dimensions Data Value Unit Outer dimensions (L x W x H) 925 x 620 x Weight approx. 105 kg ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
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Transport and storage Storage conditions WARNING! Warning - Impermissible ambient temperature! If the ambient temperature is outside of the permis- sible ambient temperature range for the samples, these might be compromised. – Observe the permissible maximum and min- imum ambient temperatures for setting up the device.
Commissioning Commissioning Set up and level the device at a suitable location so that it is stable. During setup, the required safety zone of textvar object does not exist around the device must be complied with in acc. with textvar object does not exist. WARNING! During a centrifugation run, no personnel, haz- ardous substances or objects may be within a...
Commissioning WARNING! Warning - Position changed due to vibration! The device might fall down if the set-up surface vibrates or if it is not flat or stable enough. – Only place the device on a stable, flat surface. – The set-up surface must reliably bear the weight of the device.
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Commissioning Personnel: Authorized skilled personnel with special instruction When lifting up the device, note the specified weight and only lift the device with a suitable number of helpers to avoid injuries. WARNING! Warning - Position changed due to vibration The device might fall down if the set-up surface vibrates or if it is not flat or stable enough.
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Commissioning WARNING! Warning - Impermissible ambient temperature! If the ambient temperature is outside of the permis- sible ambient temperature range for the samples, these might be compromised. – Observe the permissible maximum and min- imum ambient temperatures for setting up the device.
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Commissioning Switching on the device Personnel: Authorized skilled personnel with special instruction Switch the device on. Fig. 10: Device switch Device switch [ON/OFF] ð The control will start up. On the display, the device type, PROG T/°C t/min the software version and (depending on the setting) the 1 20 9/;\9 1500 2:00...
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Commissioning Fig. 12: Transport securing device Transport securing device NOTICE! Before commissioning the device, remove the transport securing device from the inte- rior. Remove the transport securing device. NOTICE! Check to make sure the rotor is mounted cor- rectly before commissioning. The device is now ready for operation.
Operation Operation 6.1 Operating elements START PROG T/°C t/min 4 9/;\9 1500 2:00 STOP PROG T/°C TIME OPEN Fig. 13: Control panel ‘Current program’ display segment ‘Temperature’ display segment ‘Start-up and run-down parameters’ display segment ‘Relative centrifugal force’ display segment ‘Speed’...
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Operation [TIME] key TIME Runtime, parameter t/hms. h: hours. Can be set from 1 h to 99 h, in 1-hour increments. m: minutes. Can be set from 1 min to 59 min, in 1-minute incre- ments. s: seconds. Can be set from 1 s to 59 s, in 1-second incre- ments.
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Operation [Start-up and run-down parameters] key Start-up levels, parameter _/ Level 9 = shortest start-up time, ... level 1 = longest start-up time. Start-up time, parameter / t. Adjustable in 1-second incre- ments. The adjustable time range depends on the set speed. It is only possible to set start-up times when these are acti- Ä...
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Operation [PROG] key PROG Call up programs and program links, parameter ‘RCL’ . Programs: Program locations 1 to 98 and PREC. Program links: Program locations A to Z. Save programs and program links, parameter ‘STO’ . 99 programs can be saved (program locations 1 to 98 and PREC).
Operation 6.2 Opening the lid Personnel: Authorized skilled personnel with special instruction The lid can only be opened if the device is switched on and the rotor is stopped. If it is not possible to open the lid, actuate the emergency Ä...
Operation 6.5 Loading the rotor 6.5.1 Loading a rotor for classical centrifugation Standard centrifuge containers made of glass can be stressed up to RZB 4000 (DIN 58970 Part 2). Check the rotor to make sure it is seated firmly. For swing-out rotors, all rotor places must be occupied with the same hangers.
Only use containers approved by Hettich and do not exceed their maximum rotational speeds. If not all positions of the adapter are used, the same numbers of sample containers must be distributed over the two adapters, whereby only one (mirrored) symmetrical arrange- ment may exist inside the adapter.
Operation NOTICE! You can also use compensation containers, but you should fill these with a suitable sample mate- rial so that they cannot overheat. Ideally, this is a blank sample identical to the samples being pro- cessed. Never use compensation containers filled with water, since liquids with low viscosity can strongly heat up even at low speed, which would result in the bursting of the containers, and therefore to...
Operation 6.5.3.1 Opening and closing the lid of bio-safety systems 6.5.3.1.1 Lid with screw closure To close the lid, proceed as follows: Place the lid on the hanger. Tightly seal the lid by hand by turning it clockwise. To open the lid, proceed as follows: Turn the lid by hand counterclockwise until the lid can be removed from the hanger.
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Operation Rotor detection is carried out after each centrifugation run is started. If the rotor was changed, the centrifugation run is aborted after PROG T/°C t/min rotor detection. The rotor code (rotor), the maximum rotor speed Rotor 4 Nmax= 1500 R=184 mm (Nmax) and a centrifuging radius (R) of the newly detected rotor are displayed, e.g.
Operation Short-term centrifugation A short-time centrifugation is not possible if pro- gram linkages are being used. The run-down is performed with the selected run- Ä Chapter 7.2.2.2 ‘Braking down parameters stage and run-down time’ on page 58. To perform short-term centrifugation, proceed as follows: Ä...
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Operation Required validation steps: Evaluation of a potential hazard, such as the overheating of the sample or sample container, gas formation from the sample, breakage of the sample container due to mechanical, thermal or chemical overload. To do this, the device parameters are to be increased in small increments in order to be able to notice potential hazards early enough.
Operation Monitor the device until the set speed has been reached. Check whether unbalance can be observed through the sight glass in the device lid. Start the run by pressing the [START] key. NOTICE! What to do in the event of unbalance The ZentriMix is equipped with an unbalance switch like every commercially available laboratory centrifuge.
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Operation To release the lid in an emergency, proceed as follows: Switch off the device and pull out the mains plug. Make sure the rotor is stopped by looking through the viewing window in the lid. Fig. 15: Viewing window in the lid (figure is an example) Insert the Allen key (included in delivery as an accessory) horizontally into the emergency release opening on the front...
Operation 6.9 Removing and installing the rotor Personnel: Authorized skilled personnel with special instruction To remove the rotor, proceed as follows: Unlock and open the lid Ä Chapter 6.2 ‘Opening the lid’ on page 35. Switch off the device with the device switch. Fig.
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Operation Carefully remove the rotor upward and out of the device. ð The interior (centrifuge chamber) is now accessible. NOTICE! If the rotor is incorrectly installed or fastened, it could end up coming loose. When installing the rotor, make sure that the driver of the rotor shaft is sitting correctly in the rotor groove.
Operation Place the adapter (in the case of rotor for dual centrifugation) into the rotor cavities. Ä Chapter 5.2 ‘Setting up, con- Put the device into operation necting and switching on the device’ on page 26. Fig. 22: Rotor cavity symmetrically arranged 6.10 Cooling...
Software description Software description All settings and queries are carried out via the key- board. The corresponding menus are selected by pressing keys or key combinations. Program settings Centrifugation parameters *** Machine Menu *** Fig. 23: Main menu After switching on the device, the start screen appears. Here, the display changes between the product type of the device, the soft- ware version and, depending on the setting, the last loaded pro- gram or program location no.
Software description 7.1 Program settings Overview If parameters are changed, the program location number is displayed in parentheses ‘( )’ . This means that the centrifugation data on the display no longer agrees with the stored centrifugation data of the program location. Fig.
Software description The write protection can be activated/deactivated as follows when PROG T/°C t/min the rotor is at a standstill: 1 20 9/;\9 1500 2:00 Call up the program for which you would like to activate/ PROG T/°C t/min Ä Chapter 7.1.1 ‘Calling up/ deactivate write protection 5 20 9/;\9 1500...
Software description Use the [adjusting knob] to set the desired program location. PROG T/°C t/min RCL 5 If a ‘+’ is displayed after the program loca- tion, the data is write-protected. In this case, the write protection has be removed first before saving is possible Ä...
Software description 7.1.3 Calling up/loading a program link (RCL A-Z) PROG T/°C t/min If ‘N >ROTOR MAX in Prog’ , ‘Runtime 00:00 in N > ROTOR MAX in Prog 5 Prog’ , ‘Empty Program’ or ‘Ramp Unit Time in Prog’ is displayed for program links, a centrifuga- PROG T/°C t/min...
Software description 7.1.4 Editing a program link (EDIT A-Z) It is possible to save 25 program linkages (program locations A to Z; there is no program location J). A program linkage can consist of no more than 20 programs. The speed adjustment from one program to the next one is always done in a program linkage with the start-up parameter of the next program.
Software description Repeat the previous steps until all desired programs are linked. After the last program, add a program with ‘END’ . For program linkages which consist of 20 programs, program, ‘END’ cannot be set after the 20th Program and can be omitted in this case.
Software description 7.2 Centrifugation parameters 0 9/;\9 1:00 Fig. 25: Centrifugation parameters If no key is pressed for 8 seconds after selection or during parameter input, the previous values are shown on the display. The parameters must then be input again. If multiple parameters are entered, the [START] key may only be pressed after setting the last parameter in order to accept all changes.
Software description 7.2.1 Temperature (t/°C) The temperature can only be set for devices with a cooling unit. The temperature can be input in degrees Celsius (°C) or degrees Fahrenheit (°F). Setting the temperature unit Ä Chapter 7.3.5.4 ‘Temp Unit’ on page 76. If degrees Fahrenheit (°F) is set as the temperature unit, the letter ‘F’...
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Software description PROG T/°C t/min If a start-up time is set which is longer than the run- Acc time > Run time ning time, no centrifuge run can be started. ‘Acc time > Run time’ is displayed Ä Chapter 9 ‘Trou- bleshooting’...
Software description Press the [TIME] key. PROG T/°C t/min ;\t = 00:11 R7..8 R - Indicates which start-up level corresponds to the set start-up time. Set the run-down time with the [adjusting knob]. PROG T/°C t/min ;\t = 00:15 R6..7 To accept the settings, press the [START] key.
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Software description Press the [RCF] key. PROG T/°C t/min 20 9/;\9 > 4731< 2:00 ð The LED in the [RCF] key will light up and the RCF value will be displayed Press the [RCF] key. PROG T/°C t/min RAD = <209> RCF = 4731 Set the centrifuging radius (RAD) with the [adjusting knob].
Software description To query the integral RCF, proceed as follows: PROG T/°C t/min 1 20 9/;\9 1500 2:00 Press the [RCF] key. PROG T/°C t/min RAD = <209> RCF = 4731 ð The LED in the [RCF] key is illuminated. Press the [RCF] key until the integral RCF is displayed.
Software description Maximum speed 4000 RPM, max- imum load 300 g, actual load 350 If in doubt you should obtain clarification from the manufacturer. PROG T/°C t/min If the speed in the activated program is higher than N > ROTOR MAX the maximum rotor speed (Nmax), no centrifuga- tion run can be started.
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Software description To set the runtime, proceed as follows: PROG T/°C t/min 1 20 9/;\9 1500 2:00 Press the [TIME] key. PROG T/°C t/min t/hms < 1m> ð The value shown in parentheses can be changed. Set the minutes with the [adjusting knob]. PROG T/°C t/min...
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Software description With the [adjusting knob], set ‘Timing begins at Start’ / PROG T/°C t/min ‘Timing begins at Speed’ . Timing begins at Speed ‘Timing begins - The runtime timer begins immediately at Start’ after the centrifugation run starts. PROG T/°C t/min Timing begins at Start...
Software description Press the [STOP/OPEN] key to end the centrifugation run. ð The run-down is performed with the selected run-down Ä Chapter 7.2.2.2 ‘Braking stage and run- parameters down time’ on page 58. The run-down parameter is dis- played, e.g. ‘9’ . The right LED in the [STOP/OPEN] key lights up.
Software description 7.3 Machine Menu General device settings can be made in the ‘Machine Menu’ . To call up the ‘Machine Menu’ , proceed as follows: Fig. 26: Menu structure ‘Machine Menu’ Switch on the device Ä Chapter 5.2 ‘Setting up, connecting PROG T/°C t/min...
Software description 7.3.1 Change Lock Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
Software description 7.3.2 Change PIN Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
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Software description Press the [START] key. PROG T/°C t/min new PIN = ---- [START] Set the new PIN with the [adjusting knob]. PROG T/°C t/min new PIN = 1234 [START] To deactivate the PIN, ‘0000’ must be set. Press the [START] key to save the setting. PROG T/°C t/min...
Change to the menu ‘Machine Menu è Info’ PROG T/°C t/min mation on page 66. -] Info Press the [START] key. PROG T/°C t/min Zentrimix 380 R ð The device model is displayed. ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
Software description Press the [PROG] key. PROG T/°C t/min Mains Voltage : 230V ð The mains voltage is displayed. Press the [PROG] key. PROG T/°C t/min Rotor 8*: Nmax= 2500 R=174 ð The rotor code (rotor), the maximum rotor speed (Nmax) and the preset centrifuging radius (R) of the rotor last detected by the rotor detection function are displayed.
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Software description The device is equipped with a cycle counter, which counts the run- Ä Fur- ning cycles (centrifugation runs) of the various rotor codes ther information on page 39. In the case of swing-out rotors, the cycle counter is used to register the running cycles (centrifugation runs) of the hanger.
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Software description Press the [START] key to save the setting. PROG T/°C t/min Store max cycles... Resetting the cycle counter to 0 / entering maximum permissible number of running cycles The cycle counter can be reset to ‘0’ as follows, or the maximum permissible number of running cycles can be entered.
Software description 7.3.5 Settings 7.3.5.1 SOUND / BELL Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
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Software description 7.3.5.2 SOUND / BELL error Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
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Software description To set what centrifugation data is to be displayed at switch-on, pro- ceed as follows: Ä Further Change to the menu ‘Machine Menu è Settings’ PROG T/°C t/min information on page 66. -] Settings Press the [PROG] key until the ‘Start program’ parameter is PROG T/°C t/min...
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Software description [STOP/OPEN] key textvar object does not exist ⇒ textvar object does not exist 7.3.5.5 Ramp Unit Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
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Software description Activate/deactivate the display of the integral RCF as follows: Ä Further Change to the menu ‘Machine Menu è Settings’ PROG T/°C t/min information on page 66. -] Settings Press the [PROG] key until the ‘RCF Integral’ parameter is PROG T/°C t/min...
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Software description Press the [PROG] key until the ‘Multi programs’ parameter is PROG T/°C t/min displayed. Multi programs = on Set ‘off’ or ‘on’ using the [adjusting knob]. ‘off’ - Program link deactivated ‘on’ - Program link activated Press the [START] key to save the setting. PROG T/°C t/min...
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Software description The delay time can be set as follows: Ä Further Change to the menu ‘Machine Menu è Settings’ PROG T/°C t/min information on page 66. -] Settings Press the [PROG] key until the ‘Cool acc time’ parameter is PROG T/°C t/min...
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Software description 7.3.5.12 Dual time mode Queries and settings are only possible when the rotor is at a standstill. You can jump back to the previously displayed parameter by pressing the [T/°C] key. The operation can be ended at any time by pressing the [STOP/OPEN] key.
Cleaning, disinfection and maintenance Cleaning, disinfection and maintenance DANGER! Danger due to insufficient cleaning! In the case of insufficient cleaning or non-observ- ance of the cleaning regulations, there is a risk of contamination for the user. – Observe the specified cleaning regulations. –...
Cleaning, disinfection and maintenance 8.1 Cleaning Cleaning Personnel: Authorized skilled personnel with special instruction NOTICE! To clean the device, use a mixture of anionic and non-ionic surfactants in an aqueous, multivalent alcohol base, which generate a nearly neutral (pH 7 +/-) concentrate. Do not use any cleaning agents for cleaning, such as caustic alkalis, peroxides, chlorine compounds, acids or bases.
Cleaning, disinfection and maintenance Clean the removed rotor of the device. Ä Chapter If disinfection is not necessary, remount the rotor 6.9 ‘Removing and installing the rotor’ on page 45. Other- Ä Chapter 8.2 ‘Disinfection’ wise, continue with disinfection. on page 85. Fig.
Check by a service technician from the manufacturer. Service technician play In the case of swing-out rotors, the lifting lugs must be greased regularly (Hettich lubricating grease no. 4051), to ensure the hangers swing out evenly. ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
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Cleaning, disinfection and maintenance To grease the lifting lugs, proceed as follows: Check whether the lifting lugs have an adequate film of grease. Apply a thin film of grease to the lifting lugs. Fig. 31: Greasing the lifting lugs Lifting lugs ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
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Cleaning, disinfection and maintenance ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
Troubleshooting Troubleshooting If the error can't be remedied using the trouble- shooting table, notify Customer Service. Please specify the type of device and the serial number. Both numbers can be found on the name plate of the device. Fault description Cause Remedy No display...
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Troubleshooting Fault description Cause Remedy Ä Chapter 9.1 ‘Per- CONTROL-ERROR Error, defective electronics. Perform a MAINS-RESET 22, 25.1–25.4; SER forming a mains reset’ on page 91. If the error per- I/O - ERROR 31, 34, sists after a mains reset, contact a service techni- 36;...
Troubleshooting Fault description Cause Remedy N > ROTOR MAX The rotor was changed. The Set a speed (up to the maximum speed of the previ- installed rotor has a higher ously used rotor). Press the [START] key to detect maximum speed than the the rotor.
Technical data Technical data Model ZENTRIMIX 380 R Type 3200 3200-01 Mains voltage (± 10%) 200-240 V 1~ 110 – 127 V 1~ Mains frequency 50 – 60 Hz 60 Hz Connected load 1400 VA 1600 VA Current consumption 6.6 A 15.0 A...
Technical data Height 418 mm 418 mm Weight approx. 81,5 kg approx. 89 kg 10.1 Type plate Fig. 32: Type plate Manufacturer logo Data matrix code (for internal purposes) IVD symbol CE mark with the identification number of the notified body involved in the conformity assessment process Country of manufacture Year of manufacture...
Disposal Disposal Before disposal, the device must be decontaminated and cleaned to protect people, the environment and property. Before disposing of the device, the respective legal regulations must be observed. According to the directive textvar object does not exist, devices may no longer be disposed of in the household waste.
Glossary Glossary Dual centrifugation In dual centrifugation, one or more sample containers is cen- trifuged, each of which rotates additionally about another axis. This special kind of centrifugation leads to a fundamen- tally different result than classical centrifugation. While classical centrifugation is a separation method, dual centrifugation is a method for mixing, homogenizing or dis- persion.
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Rotors and accessories Tab. 3: Bio-seal Rotor Hanger/insert/adapter Bio-seal 3205 3212 + 3216 / 3217 3206 3212 + 3216 / 3217 3218 3220 3221 3234 1752 1751 Tab. 4: Maximum load, maximum number of running cycles and period of use Rotor Maximum load [g] Maximum number of...
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Rotors and accessories Lowest reachable temperature at maximum speed, 1 h running time and 20°C room temperature ZENTRIMIX | 380 R | en_GB | 2 | AB3200EN...
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