Preparing The Lc System; Entering Settings - Agilent Technologies 1100 Series User Manual

Running a Gradient Analysis

Preparing the LC System

Preparing the LC System
1 Fill one solvent bottle with bidistilled water (channel A) and the other with
acetonitrile (channel B).
2 Turn on the detector lamp and pump using the On/Off button [F7] in the Analysis
screen.
3 For the quaternary pump, turn on the degasser by pressing the line-power switch.
4 Purge the pump. For more information see Chapter 3 "Using the Pump".
5 Allow the detector at least 15 minutes to provide a stable baseline.
6 Fill the contents of a Agilent Technologies isocratic standard sample ampoule into a
vial and seal the vial with a cap. Place the vial in position 1 of the autosampler tray.
7 Pump the water/acetonitrile (35/65 %) mobile phase through the column for
10 minutes at a flow rate of 2 ml/min.

Entering Settings

To set up the gradient analysis you will set the LC system settings to default and
then modify selected settings, the other settings will remain with their default
values. You will then save these settings to a method called GRAD.
To set up the solvent gradient, you will create a timetable in the method. This is
done using the Timetable screen, accessed by selecting the Timetable button [F2] in
the Pump settings screen.
The example assumes that the Timetable is empty. If the Timetable is not empty, use
the All Lines item, accessed by pressing the Delete button.
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