Slit Width - Agilent Technologies G1315B User Manual

Figure 30

Slit Width

The detector has a variable slit at the entrance of the spectrograph. This is an
effective tool to adapt the detector to changing demand of different analytical
problems.
A narrow slit provides spectral resolution for analytes with very fine
structures in the absorbance spectrum. An example of such a spectrum is
benzene. The five main absorbance bands (fingers) are only 2.5 nm wide and
just 6 nm apart from each other.
1200 Series DAD and MWD User Manual
Grad.: 0.02 m KH PO /ACN from 12% ACN to 45% ACN in 12 min
2 4
Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Refer-
ence
Time (min)
How to optimize the Detector
1 pmol each
Wavelength
267 nm
Reference 380 nm
Wavelength
267 nm
No reference
5
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