Agilent Technologies Cary 8454 Operator's Manual page 90

5 Using your UV-Visible Spectroscopy System
Finding the Caffeine Absorbance Maximum
6 Press the Blank button on the front of the spectrophotometer or click Blank
7 Remember the orientation of the cell in the cell holder. Lift the level to
8 Press the Sample button on the front of your spectrophotometer or click
90
on the Instrument Panel to start the measurement.
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample. Then fill the cell with about 3 ml of the caffeine sample.
Make sure that the cell windows are clean, and reposition the cell in the
same orientation as for the reference measurement. Close the cell holder's
level.
Sample on the Instrument Panel to start the measurement.
Agilent Cary 8454 UV-Visible Spectroscopy System Operator's Manual