Agilent Technologies Cary 8454 Operator's Manual page 100

5 Using your UV-Visible Spectroscopy System
Quantitative Analysis using a Calibration with Standards
4 Setup your analysis wavelength at 273 nm (Use wavelength), type Caffeine
5 Click OK to set your parameters.
Now you are ready to run your measurements.
NOTE
100
for the Analyte name, set the Calibration curve type to Linear, select
Concentration entry and use mg/l as Unit. Check the Prompt for standard
information and the Prompt for sample information. Select Absorbance as
Data type and set Display spectrum From 190 nm To 340 nm.
Agilent Cary 8454 UV-Visible Spectroscopy System Operator's Manual