Solvent Information; Flow Cells; Solvents - Agilent Technologies Cary 8454 Operator's Manual

Uv-visible spectroscopy system
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Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.

Solvent Information

Organic and aqueous solvents can be hazardous if handled improperly. Always follow
CAUTION
the precautions listed below to protect both operator and instrument.

Flow Cells

Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus
impair the optical properties of flow cells.

Solvents

Always filter solvents, small particles can permanently block capillaries. Avoid
the use of the following steel-corrosive solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
• High concentrations of inorganic acids like nitric acid, sulfuric acid
• Halogenated solvents or mixtures which form radicals and/or acids, for
Agilent Cary 8454 UV-Visible Spectroscopy System Operator's Manual
iodide, potassium chloride, and so on).
especially at higher temperatures (replace, if your analysis method allows,
by phosphoric acid or phosphate buffer which are less corrosive against
stainless steel).
example:
2CHCl3 + O2
2COCl2 + 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
Safety Information
Precautions
13
1

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