BIO RAD DCODE Manual page 14

Model 475 System Components
Syringe
Plunger Caps/Plunger Cap Screw There are two plunger caps, one for each syringe. The
Lever Attachment Screw
Syringe Sleeve
Syringe Holder/
Syringe Holder Screw
Volume Adjustment Screw
Volume Setting Indicator
Lever
Tygon Tubing
Y-Fitting
Luer Fitting/Coupling
Plunger cap
screw
Lever
attachment
screw
Fig. 3.11. Plunger Cap
Description (DGGE System only)
Two pairs each of 10 and 30 ml disposable syringes are
provided. The 10 ml syringes are for gel volumes less than
10 ml. The 30 ml syringes are for gel volumes greater than
10 ml. For proper gel casting, use matching syringe sizes.
plunger caps fit both the 10 and 30 ml syringes (Figure 3.11).
The lever attachment screw is on the plunger cap. This
screw fits into the groove of the lever and conducts the
driving force of the cam in dispensing the gel solution.
One pair of syringe sleeves for each size syringe is provided
(Figure 3.12). The sleeve is a movable adaptor to mount the
syringe in the holder. The sleeve should conform to the
syringe. If the syringe is too tight or too loose, adjust the
sleeve by pushing or pulling.
The syringe holder is next to the lever. It holds the syringe in
place and controls the delivery volume. The syringe is held
in the holder by tightening the holder screw against the sleeve.
The volume adjustment screw is on both sides of the syringe
holder (Figure 3.10). It adjusts the holder to the desired
delivery volume.
The volume setting indicator is at the top corner of the syringe
holder nearest the volume setting numbers (Figure 3.10).
The position of the lever is controlled by the rotation of
the cam (Figure 3.10). The lever must be in the vertical or
start position before use.
One length of Tygon ™ tubing is provided. Cut the tubing into
two 15.5 cm and one 9 cm lengths. The longer pieces are used
to transport the gel solution from the syringes into the Y-fitting.
The short piece will transport the gel solution from the
Y-fitting to the gel sandwich.
The Y-fitting mixes the high and low density gel solutions
(Figure 3.13).
There are two luer fittings that fit both 10 and 30 ml syringes.
The fittings twist onto the syringe and connect to the Tygon
tubing on the other end. A luer coupling is used on one end
of the 9 cm tubing to connect it to the gel sandwich stopcock.
Fig. 3.12. Syringe sleeve
10
Fig. 3.13. Y-Fitting