Optical Flow Cell Wetting Procedure - CELL-DYN 3200 Service Manual

8#0#9HULILFDWLRQ#3URFHGXUHV •
VP-45 Optical Flow Cell Wetting Procedure
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.
Required Tools & Materials:
• CN Free HGB/NOC Lyse
• 10 mL Syringe
Verification Procedure:
1. Fill syringe with HGB lyse solution.
2. From Main Menu, press
„ SPECIAL PROTOCOLS
„ EMPTY/FILL FLOW CELL
„ EMPTY FLOW CELL
3. From Main Menu, press
„ DIAGNOSTICS
„ MORE
„ SOLENOID OPERATION
4. To open solenoid 56, press
„ STEP SOLENOID
type 56 , and press
„ ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
®
CELL-DYN 3200 SYSTEM SERVICE MANUAL
VP-45 Optical Flow Cell Wetting Procedure
6. Inject HGB lyse reagent in short strokes to create turbulent
motion in flow cell.
7. Let lyse remain is flow cell for three to five minutes.
8. Disconnect syringe, and connect reconnect elbow to
solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
≤ µ
WBC 0.10 K/
11. Refer to Figure 5-16. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, repeat this
procedure.
Figure 5-16 Flow Cell Bubbles
Fe w S m a ll A ir
B ub b le s a re O K
−
Part Number 9140300 August 1998
≤ µ
, RBC 0.02 M/ L, and PLT
L
To W a ste /
Va cu u m
C e ll
S tre a m
N O L a rg e
A ir B u b b le s
S he a th P rim e
S he a th In
3 2 0 90
S am p le In
≤ µ
5 K/ L.
5 - 45