CELL-DYN 3200 Service Manual
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CELL-DYN 3200 Service Manual

Automated hematology analyzer
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• 1 - Introduction
• 2 Troubleshooting
• 3 - Parts Lists
• 4 - Removal & Replacement
• 5 - Verification Procedures
®
CELL-DYN
3200
SYSTEM

SERVICE MANUAL

Part Number 9140300

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Summary of Contents for CELL-DYN 3200

  • Page 1: Service Manual

    • 1 - Introduction • 2 Troubleshooting • 3 - Parts Lists • 4 - Removal & Replacement • 5 - Verification Procedures ® CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300...

  • Page 2: Table Of Contents

    Section 6: Preventive Maintenance & Total Call..3 CELL- DYN is a registered trademark of Abbott Laboratories. 1.4 CELL-DYN 3200 Operator’s Manual ....3 ® Vacutainer is a registered trademark of Becton-Dickinson and 1.5 Warranty Information.

  • Page 3: Revision Log

    Table 1-1 Revision Log Revision Date Pages Revised/Added TSBs Incorporated ISAs Incorporated August Complete Revision 109 - 001A, 003 109 - 003, 004, 005, 006 − 1 - 2 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 4: About This Manual

    Operating instructions and routine maintenance procedures for This section contains procedures to be used by the FSR in the CELL-DYN 3200 are included in the Operator’s Manual identifying and isolating problems. The procedures are provided with each system. It is essential that the service presented in a flowchart format and are accompanied by engineer read and understand the Operator’s Manual, and be...

  • Page 5: Warranty Information

    1.5 Warranty Information System External Components Components comprising the CELL-DYN 3200 System have The following is a list of the CELL-DYN 3200 external been designed by Abbott Laboratories for optimal performance. components and conditions: Substitution of reagents, accessories, or system components •...

  • Page 6: System Internal Components

    4#0#,QWURGXFWLRQ • 1.7 Safety System Internal Components Signal Words and Graphic Warning Conventions The following is a list of the CELL-DYN 3200 internal components: Signal Words • CELL-DYN 3200 System Components '$1*(5=##Denotes an immediate hazard which, if not – Flow Panel avoided, could result in serious injury or death.

  • Page 7: Biosafety

    Bloodborne Pathogen Rule (29CFR Part 1910.1030) or other equivalent biosafety procedures. :$51,1*=#327(17,$/#%,2+$=$5'#The aspiration needle/probe is sharp and potentially contaminated with infectious materials. Avoid contact with the tip of the needle/probe. − 1 - 6 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 8: Electrical Safety

    If the instrument is used or modified in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired. − ® 1 - 7 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 9 1- 2 at a wavelength of 632.8 nm. During open beam configurations, assure that the beam is confined to the laser bench area and only personnel with proper eye protection are present. − 1 - 8 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 10: Cell-Dyn 3200 Hematology Parameters

    4#0#,QWURGXFWLRQ • 1.8 CELL-DYN 3200 Hematology Parameters 1.8 CELL-DYN 3200 Hematology Parameters .CUGT 9CTPKPI The following tables list the CELL-DYN 3200 Hematology .CDGN Parameters and their abbreviations. Table 1-2 Red Cell Parameters Full Name Abbreviation Red Blood Cell Concentration Hemoglobin Concentration...

  • Page 11: System Specifications

    4#0#,QWURGXFWLRQ • 1.9 System Specifications Table 1-3 White Cell Parameters (Continued) 1.9 System Specifications The following tables contain the specification for the CELL-DYN Full Name Abbreviation 3200 System. Eosinophil Percentage Table 1-5 Physical Specifications (Unpackaged) Basophil Concentration BASO Basophil Percentage...
  • Page 12 Monitor 100 - 240 VAC 50/60 1.3 amps µ ± 120 VAC ≤ ≥ 5.0% 0.72 2.0-18.0 fL Printer 50/60 0.5 amps 220 - 260 VAC − 1 - 11 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 13 Open 80 Samples/Hour Closed 68 Samples/Hour • 2-inch maximum label length • 1.25-inch maximum label width Whole Blood Aspiration Volume µ Open Mode µ Closed Mode − 1 - 12 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 14 Blank Display......2-76 IP-24 HSSL Failures/Message Reception Time-Out . . . 2-77 2 - 1 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998...

  • Page 15: Troubleshooting Approach

    2.1 Summary c. To refer to the appropriate IP or VP, click on the red link. This chapter contains the following information for CELL-DYN d. Begin at the START box and follow the directions in the 3200: e. If a part is adjusted or replaced, go to Section 5.2,...

  • Page 16: Error Messages

    HGB Errors IP-13 IP-27 Position Histogram Errors IP-9 Rack Bar Code Read Failure IP-27 HSSL Com Failures IP-24 Shear Valve Position Fault IP-20 Incomplete Aspiration IP-3 ® 2 - 3 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 - August 1998...

  • Page 17: Error Messages

    Fresh. Differential count is not reliable WBC Imprecision before 30 minutes or after 12 hours after sample is drawn. WBC/HGB Lyse Empty IP-7 X-B Errors IP-14 ® 2 - 3A CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 - August 1998...

  • Page 18: Fluid Leaks, Open Mode Probe Or Wash Block

    - Verify connection on top - Verify Shear valve of Probe fittings - Replace Y-Valve (PL-8) - Clean Shear Valve Y E S (VP-48) - Replace Shear Valve (PL-9.1) 2 - 4 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300-August 1998...
  • Page 19 - Ensure tubng between WC #4 and Valve 31 is not restricted - Verify operation of Valve 31 and replace if necessary (VP-10), (PL-4.1) - Replace pinch tubing in Valve 31 (TM-1) − 2 - 5 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 20: Fluid Leaks, Closed Mode Needle Or Wash Block

    - Verify Shear valve - Verify connection on top fittings of Probe - Clean Shear Valve - Replace Y-Valve (PL-8) (VP-48) - Replace Shear Valve Y E S (PL-9.1) − 2 - 6 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 21 - Ensure tubng between WC #4 and Valve 31 is not restricted - Verify operation of Valve 31 and replace if necessary (VP-10), (PL-4.1) - Replace pinch tubing in Valve 31 (TM-1) − 2 - 7 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 22: Incomplete Aspiration

    Rotating Properly Mode(s) Affected Problem Open Only Both Corrected Closed Only - Replace Y-Valve Assy (PL-8) - Replace MDM (Pl-9.2) - Replace SDM/CDM (PL-15) Total Call − 2 - 8 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 23 (VP-10) - Replace Open Probe (PL-8). ensure Vent Trap plumbing is Y E S free of restrictions. - Check the Closed Probe plumbing for air leaks. − 2 - 9 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 24 - Verify Vac 2 level (VP-16). Troubleshoot as necessary. - Ensure there are no restrictions between Y-Valve and Valve 12 - Verify Valve 12 and tubing (VP-10). Replace as necessary (PL-4.1), (TM-1). − 2 - 10 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 25: Flow Errors

    - Observe the Delivery Syringe WC1 at a fast and constant rate and verify smooth upward (See Figures). movement (See Figures). Delivery Syringe Dripping Smooth Movement Y E S − 2 - 11 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 26 Stable (PL-4.2). - Remove, inspect, and clean Flow Cell Nozzle. - Replace Flow Cell Assy. (PL-10). - Replace Laser (PL-10). - Replace Laser Power Supply (PL-2.2). − 2 - 12 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 27: Rbc/Hgb Diluent Overpressure

    (PL-4.1) „ SHEAR VAL TIME Y E S - Replace Sensor (PL-4.2) Times Problem Corrected Y E S Total Call Y E S Total Call − 2 - 13 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 28 - Replace defective section: Front (PL-9.1) Rear (PL-9.1) Rim Notch If problem persists: Faces Right Total Call - Replace Shear Valve Driver (PL-9.1) 32002 Total Call − 2 - 14 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 29: Optical Parameter Count Problems

    „ F R A G I L E W B C - Run a Normal Control five times and record the optical values. - Compare the results to the Data Sheet. − 2 - 15 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 30 RBC & PLT Y E S Only Low RBC & PLT Y E S Only High W B C O n l y Y E S − 2 - 16 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 31 W B C O n l y Y E S Y E S All Erratic Erratic NOC Only RBC & PLT Y E S Y E S Erratic Only Erratic Total Call − 2 - 17 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 32 If good, replace Laser Head (PL-10). If not, replace Laser Power Supply (PL-2). Problem Corrected 'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#LQVWUXPHQW#LV# 'DQJHU 'DQJHU 'DQJHU RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#FRQQHFWRU# ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#6KRUW#PDOH#SLQV# Y E S WR#&KDVVLV1 Total Call − 2 - 18 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 33 „ SET POINT ENTRY - Replace 0 Preamp (PL-10). - Replace SPM (PL-10). - Verify WBC Threshold is 400. - Replace CPU/DCM (PL-13). - Perform NOC Gain Adjustment (VP-25). − 2 - 19 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 34 If good, replace Laser Head (PL-10). If not, replace Laser Power Supply (PL-2). Problem Corrected 'DQJHU 'DQJHU 'DQJHU 'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#LQVWUXPHQW# LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU# FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1# 6KRUW#PDOH#SLQV#WR#&KDVVLV1 Y E S Total Call − 2 - 20 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 35 Problem - Replace MAM (PL-12). Corrected - Replace SPM (PL-10). - Replace CPU/DCM (PL-13). Problem Corrected Y E S Total Call Y E S Total Call − 2 - 21 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 36 Gain (VP-26), - Verify NOC Threshold is 400. (VP-27). - Perform NOC Gain Adjustment - Replace MAM (PL-12). (VP-25). - Replace SPM (PL-10). - Replace CPU/DCM (PL-13). − 2 - 22 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 37 - Verify WBC Gain (VP-23). - Perform NOC Gain - Replace MAM (PL-12). Adjustment (VP-25). Problem - Replace SPM (PL-10). Corrected - Replace CPU/DCM (PL-13). Y E S Total Call − 2 - 23 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 38 If good, replace Laser Head (PL-10). If not, replace Laser Power Supply (PL-2). Problem 'DQJHU 'DQJHU 'DQJHU 'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH# Corrected LQVWUXPHQW#LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ# WKH#ODVHU#FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH# PDOH#SLQV1#6KRUW#PDOH#SLQV#WR#&KDVVLV1 Y E S Total Call − 2 - 24 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 39 Corrected - From Main Menu, press Total Call „ DIAGNOSTICS „ F12 „ F1 „ SET POINT ENTRY Total Call - Verify NOC Threshold is 400. − 2 - 25 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 40: Reagent Empty

    - Ensure reagent lines are not crimped or clogged. - INITIALIZE and PRIME instrument. - Open Left Front Door Diluent/Sheath and observe Diluent and Empty Sheath Reservoirs. Total Call − 2 - 26 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 41 See Figure. Replace as - Replace Syringe Driver necessary (PL-4.1), (TM-1). Assembly (PL-7), (RR-11). 3 2 0 9 8 - Replace SDM2 (PL-15). - Replace MPM (PL-11). − 2 - 27 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 42 Y E S Past Electrode Y E S Neither Fill Filling H e re Properly Sheath Filling Past Electrode 3 2 0 9 9 Y E S − 2 - 28 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 43 - Replace FCM (PL-14.1). Replace as necessary (TM-1). - Replace FCM (PL-14.1). - Verify Reservoirs are not cracked or leaking. Replace as necessary (PL-4.2). - Replace FCM (PL-14.1). − 2 - 29 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 44 2 and 3. 3 2 1 0 0 - Reseat Sensor Cables (CDM 2 J8 for Diluent, or CDM 2 J10 for Sheath. - Replace FCM (PL-14.1). − 2 - 30 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 45: Optical Backgrounds Too High

    - Clean/replace WBC Syringe (PL-7). - Ensure WBC Mixing Chamber is being rinsed and drained properly. Y E S - INITIALIZE and PRIME Instrument. Total Call − 2 - 31 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 46: Histogram Problems

    Diluent for RBC/ Syringes (PL-7). PLT problem. - Replace In-line Sheath Filter (PL-4.2). Problem Corrected Problem Corrected Y E S Total Call Y E S Total Call − 2 - 32 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 47: Controls Out

    „ S E T U P Y E S „ Q.C. SETUP MENU „ Q.C. LIMITS Total Call - Edit as necessary. Go to (IP-8). - Run Q.C. − 2 - 33 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 48 „ SPECIMEN TYPE (VP-58). MCH = HGB X10 - Select empty Q.C. File „ Q.C. SPECIMEN MCHC = HGB X 100 - Review CV% (VP-57). MCV X RBC − 2 - 34 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 49: Vacuum Accumulator #1 Wet

    „ D R A I N A C C U M U L A T O R S - Perform Vacuum Accumulator #2 Rinsing Procedure (VP-60) Y E S Total Call − 2 - 35 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 50 Megohms. See Figure Vac 1 Acc Vac 2 Acc V P M > 20 Megohms Y E S 32003 - Replace VPM (PL-5) - Replace FCM (PL-14.1) − 2 - 36 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 51: Vacuum Accumulator #2 Wet

    „ D R A I N A C C U M U L A T O R S - Perform Vacuum Accumulator #2 Rinsing Procedure (VP-60) Y E S Total Call − 2 - 37 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 52 Megohms. See Figure Vac 1 Acc Vac 2 Acc V P M > 20 Megohms Y E S 32003 - Replace VPM (PL-5) - Replace FCM (PL-14.1) − 2 - 38 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 53: Hgb Problems

    (VP-10) and Reference Readings. and replace as necessary (PL-4.1). - Verify operation of HGB Lyse and RBC Diluent Syringes (VP-6), (VP-7) and replace as necessary (PL-7). − 2 - 39 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 54 1950-2050 ≈ 1600 1950-2050 Erratic Erratic ≈ 0 ≈ 0 - Verify tubing, operation (VP-10), and flow through Valve 94 and replace as necessary (TM-1), (PL-4.1). − 2 - 40 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 55: X-B Errors

    - Review Q.C. Logs for all 3 Control Levels. - Verify RBC, HGB, and MCV Control Levels are not at the high or low end of range. − 2 - 41 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 56 Data Points (100 Procedures Samples). performed before X-B X-B In Shift. Range - Monitor X-B for 5 Data Points (100 Samples). X-B In Range Total Call Total Call − 2 - 42 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 57 - Verify Calibrator Insert Sheet matches Current Calibrators. - Perform System Calibration (VP-58) - Determine which parameter(s) are out-of- range, and go to (IP-15) (IP-16) for HGB and correct. − 2 - 43 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 58: Optical Parameters Imprecision

    - Manually clean, debubble, and wet Optical Only Flow Cell (VP-44), (VP45). Y E S - Clean Optics Bench (VP-43). Y E S - Replace In-line Sheath Filter (PL-4.2). Total Call − 2 - 44 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 59 (PL-10) - Ensure HGB Mixing - Replace SPM (PL-10) - Replace CPU/DCM (PL-13) Chamber is being rinsed and drained properly. - Replace CPU/DCM (PL-13) Total Call − 2 - 45 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 60 - Verify 0 and 10 Gain (VP-26), (VP-27). - Replace Shear Valve Sections (PL-9.1). - Replace MAM (PL-12). - Replace SPM (PL-10). - Replace CPU/DCM (PL-13). − 2 - 46 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 61: Hgb Imprecision

    „ SPECIMEN TYPE - Select empty Q.C. File „ Q.C. SPECIMEN - Run one Patient 10 times. Y E S - Review HGB CV% (VP-57). Total Call − 2 - 47 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 62: Primary Vacuum/Pressure Isolation

    6 and 7 on Air Running Supply Manifold, and observe Pumps and VPM Pump LEDs. See Figures Y E S 3 2 0 0 5 - Observe LEDs. See Figure − 2 - 48 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 63 „ D I A G N O S T I C S „ M O R E „ INITIALIZATION Y E S - Observe Flow Panel valves - Replace Power Supply (PL-1) Total Call − 2 - 49 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 64 Then Dim When Activated Fuse Y E S Blown Y E S Total Call Y E S - Replace Power Supply (PL-1) 3 2 0 0 7 − 2 - 50 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 65 - Inspect Vent Valves 1 and plumbing and 3-Way and 12" Hg plumbing for 4. Replace as necessary Valves. Replace as leaks. (PL-5) necessary (PL-18.3), - Replace VPM (PL-5) (TM-3) − 2 - 51 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 66 - Replace Pump Relay Board (PL-5) - Inspect Sample Loader plumbing and 3-Way Valves. Replace as - Pinch tubing See Figure necessary (PL-18.3), this page (TM-3) − 2 - 52 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 67 Y E S - Replace Pump Relay Board (PL-5) - Inspect Sample Loader plumbing and 3-Way - Pinch tubing. See Figure Valves. Replace as necessary (PL-18.3), (TM-3) − 2 - 53 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 68 - Check 115VAC across Pins 1 and Doesn't Y E S Y E S 2 on PRM P2. IF present, replace R u n Pres Pump. If not, replace PRM (PL-5) − 2 - 54 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 69 Cycles Cycles Verification (VP-17) < 2 min < 2 min Y E S Total Call See Pressure Leaks (IP-18) See Vacuum Leaks (IP-19) Y E S − 2 - 55 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 70 +1 2 V U R FC M S D M 9 -J1 0 U R R E T S D M D 3 2 0 11 − 2 - 56 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 71: Pressure Leak Isolation

    „ D I A G N O S T I C S Y E S „ M O R E „ INITIALIZATION Total Call - Adjust Pressure Levels if necessary (VP-16) − 2 - 57 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 72 Then Dim When Activated Fuse Y E S Blown Y E S Total Call Y E S 3 2 0 07 - Replace Power Supply (PL-1) − 2 - 58 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 73 „ S E L E C T When finished press: „ FINISH SELECT - Observe the 3 levels and verify that none decrease more than 0.2 psi in 1 minute. − 2 - 59 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 74 (PL-4.2). Pres Y E S - Inspect 5 psi Accumulator and plumbing on Air Supply. Replace as Pres necessary (PL-5), (TM-2) Y E S Total Call − 2 - 60 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 75 - Inspect 12 psi plumbing - Inspect 9 psi Accumulator and Accumulator. and plumbing on Air Replace as necessary Supply. Replace as (PL-5), (TM-2). necessary (PL-5), (TM-2) − 2 - 61 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 76 6 on Manifold. leaking stops replace as necessary (PL-4.1) Pres Y E S - Inspect Sample Loader plumbing and 3-Way Valves. Replace as necessary (PL-18.3), (TM-3) − 2 - 62 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 77 +1 2 V U R FC M verify proper operation S D M 9 -J1 0 U R R E T S D M D 3 2 0 11 − 2 - 63 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 78: Vacuum Leak Isolation

    „ D I A G N O S T I C S „ M O R E „ INITIALIZATION Total Call - Adjust Vacuum Levels if necessary (VP-16) − 2 - 64 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 79 Then Dim When Activated Fuse Y E S Blown Y E S Total Call Y E S 3 2 0 07 - Replace Power Supply (PL-1) − 2 - 65 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 80 „ S E L E C T When finished press: „ FINISH SELECT - Observe the 2 levels and verify that none decrease more than 0.2 psi in 1 minute. − 2 - 66 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 81 - Press in on Valve 12. Replace as Y E S necessary (PL-4.1). - Inspect Vac 2 Y E S Accumulator and plumbing on Air Supply. Replace as necessary Total Call (PL-5), (TM-2) − 2 - 67 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 82 - Inspect Sample Loader as necessary (PL-5), plumbing and 3-Way (TM-2) Valves. Replace as - Inspect Vent Valve 4. necessary (PL-18.3), Replace as necessary (TM-2) (PL-5) − 2 - 68 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 83 +1 2 V U R FC M verify proper operation S D M 9 -J1 0 U R R E T S D M D 3 2 0 11 − 2 - 69 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 84: Shear Valve Position Fault

    - Lubricate Shear Valve Driver (VP-42). Y E S - Replace Shear Valve Driver Assy. (PL-9.1). Total Call - Replace FCM (PL-14.1). - Replace Power Supply (PL-1). − 2 - 70 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 85 +1 2 V U R FC M S D M 9 -J1 0 U R R E T S D M D 3 2 0 11 − 2 - 71 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 86: Flags

    Go to (IP-8). - Replace RBC Diluent (RBC/ PLT Flags) or WBC Lyse Problem (WBC Flags) Container. Y E S Corrected Total Call − 2 - 72 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 87 - Replace MAM (PL-12). - Replace MAM (PL-12). - Replace SPM (PL-10). Band - Replace SPM (PL-10). - Replace CPU/DCM (PL-13). LURI - Replace CPU/DCM (PL-13). Blast − 2 - 73 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 88: External Waste Full

    - Reseat Waste Sensor Plug - Clear Fault - Insert Insulated Tool into Sensor Jack to open contacts. See Figure Problem Corrected Total Call Y E S − 2 - 74 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 89 Perform in the following order until problem is corrected: - Reseat CDM2, J7 and FCM, J2 - Replace Waste Sensor Jack (PL-3.2) - Replace CDM2 (PL-16) - Replace FCM (PL-14.1) − 2 - 75 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 90: Blank Display

    - Replace Monitor (PL- 17.3). - Troubleshoot Attach Video Cable From CRT to Laptop "dead" Analyzer External Monitor Port problem. Laptop 3 2 1 0 1 Computer − 2 - 76 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 91: Hssl Failures/Message Reception Time-Out

    - Re-seat the HSSL Board. Controller (PL-17.2) - Check and re-connect the HSSL Cable. - Reload HSSL Com (VP-67) Problem Corrected Y E S Total Call − 2 - 77 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 92 - Re-seat Power Cable on Hard Drive and CDM. Problem Corrected Problem Y E S Corrected Total Call - Replace Hard Drive (PL-17.1). Y E S Total Call − 2 - 78 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 93 R E P O R T „ STOP PRINTING „ C O N F I R M S T O P Y E S Total Call − 2 - 79 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 94 - Replace I/O Board (PL- 17.2). - Replace Motherboard (PL-17.2). Laptop Printer Prints Prints Y E S Y E S - Replace Printer. - Replace Printer Cable (PL-17.3). − 2 - 80 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 95 Rack advance Tube lost moving from position 3 to Tube Sensor Board, SHM 2 VP-38 PL-18.1, PL-18.2 position 4 Rack barcode read failure VP-40 Rack Barcode Label − 2 - 81 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 96 Parts List(s) Tube position barcode read failure VP-40 Rack Position Barcode Labels Barcode reader failure VP-33, VP-11 Barcode Reader, Aspiration Tower, SHM 2 PL-18.2, PL-6.1, PL-18.1 − 2 - 82 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 97 Table of Contents $SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP Table of Contents Figure 2A-1 Cable Connection Diagram, SL ... . 2 − 2A - 1 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 98 FLOW PANEL (X2) SDM1-4..FLOW PANEL 9520255 ASY3A *S/L=SERIAL LINK PCS PANEL SDM5,6..REAGENT PANEL CHAIN CABLE SOLENOID CHAIN CABLE LASER PANEL CONNECT THE DOOR AND PLATE TO CHASSIS GND. − 2A - 2 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 99 Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams ..20 Figure 2B-20 Normal PLT and RBC Histograms ..21 − 2B - 1 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 100 External MUX DAC Control Bus CPU/DCM Serial Peripheral Bus Serial RS-485 Bus To SHM1 (Aspiration Tower Control) 32043 Serial RS-485 Bus To SHM2 (Sample Loader Control) − 2B - 2 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 101 Figure 2B-2 ADC Block Diagram Figure 2B-2 ADC Block Diagram Display PC Module High Speed Serial Link ADC1 ADC3 ADC2 Tag Bus CPU/DCM ADC4 32044 MUX DAC Control − 2B - 3 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 102 Figure 2B-3 DAC Block Diagram Figure 2B-3 DAC Block Diagram Display PC Module High Speed Serial Link DAC1 DAC2 DAC3 CPU/DCM DAC4 MUX DAC Control 32045 − 2B - 4 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 103 10 o o Analog Out VDYN Tag Bus 90 o CPU/DCM VDYN External CPU Bus Peripheral Bus LED Current HGB Voltage Flow HGB Signal Cell 32046 − 2B - 5 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 104 1 12 psi Accumulator Vent 2 9 psi Pressure Supply 3 5 psi Pressure Supply 4 12" Hg Accumulator Vent 5 Vacuum #2 Vacuum Supply 32047 − 2B - 6 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 105 9-4 HGB Flow Cell Diluent Flush SDM 9 9-5 RBC Mixing Chamber Diluent Flush 9-6 WBC Mixing Chamber Diluent Flush 9-7 WC4 Vacuum (12" Hg) 9-8 WC4 Pressure (12 psi) 32048 − 2B - 7 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 106 Vacuum Pump Peripheral Bus Pressure Pump Limit Switch (CW) Shear Shear Valve Motor Valve Driver Limit Switch (CCW) ON/OFF Motor Y-Valve SCM/CDM Drive Motor CW/CCW Module 32049 − 2B - 8 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 107 Limit Switch (CW) SHM1 GS1 Home Door Closed Interface Ultrasonic Short Sample Board Sample Cover Y-Valve Closed Mode Sensor Interface Short Sample Board Y-Valve Open Mode Sensor 32050 − 2B - 9 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 108 Load Side Empty Sensor Power From PDM U. S. Fourth Rack Sensor U. S. Fifth Rack Sensor Mixer Home Sensor (Up) Mixer Head Home Sensor 32105 − 2B - 10 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 109 Pres #1 Pres #2 Pres #3 Vac #1 Accumulator Accumulator Accumulator Accumulator Accumulator 5" Hg 12 psi 9 psi 5 psi 12" Hg (Varies) Vent Vent − 2B - 11 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 110 AC Voltage Selector + 5, + 15, -15, GND-SHM2 Transformer 115VA C + 2400VDC LASER P/S LASER (Top) 115VAC, Rear Fan 115VAC, Side Fan 115VAC, PRM 32052 − 2B - 12 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 111 P o w e r V id e o D is p la y C RT S u p p ly M o d u le − 2B - 13 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 112 AGND Amplifiers Lin Amplifiers ADC4 Amplifier From VDYN/-100 AGND 90 PMT Preamp 32055 AGND Amplifiers Lin Amplifiers VDYN/-100 AGND 90 D PMT Preamp +10V Reference AGND − 2B - 14 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 113 On CLK 9 SDM9 V3 Mixer Lift Hi CLK CDM2 Reset Hi CLK 9 V4 Mixer Bladders Reset Reset V5 Unload Side Cross Transfer Spare Spare Spare AGND − 2B - 15 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 114 115VAC In Neutral AC Out 2 Winding Vacuum Pump Voltage Vac. Pump On Feedback (Diagnostics) Pres. Pump On AC In 1 AC Out 2 32057 Pressure Pump − 2B - 16 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 115 Typical MDM Optical Sensor Input Limit Sensor Baord Shear Valve Driver Module J4-J5 Valve CCW DGND Valve CW Valve CW DGND Valve CCW AGND RBC Overpressure Sensor 32058 − 2B - 17 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 116 Sensor-J1 M0 I0 Note: Pin configurations on the assemblies that are not 32059 M0 I1 shown are the same as those shown. M0 PH0 M0 PH1 − 2B - 18 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 117 $SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV • Figure 2B-18 Normal 10,90 & 0,90D S'grams Figure 2B-18 Normal 10, 90 and 0, 90D Scattergrams − 2B - 19 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 118 Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams 0° 10° Complexity 32103 B - Basophil L - Lymphocyte N - Neutrophil E - Eosinophil M - Monocyte − 2B - 20 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 119 Figure 2B-20 Normal PLT and RBC Histograms RBC/PLT 1. Curve Starts to the Right of the Lower Threshold. 2. Single Peak 32104 3. Good Distance From Lower Threshold at Peak − 2B - 21 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 120 Service Commands ....... . 12 − 2C - 1 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 121 VAC 2 WASTE (BLK) (VAR.) 2 WAY PINCH VALVE NORMALLY CLOSED DRAIN VAC 2 (BRN) AIR FLOW RESTRICTOR 32060 CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW − 2C - 2 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 122 Load Side Cross Transfer .016" Orifice 3-Way Valve Unload Side Cross Transfer .016" Orifice 3-Way Valve Mixer Head Up/Down .016" Orifice 3-Way Valve 5 psi Mixer Bladders Regulator 32061 − 2C - 3 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 123 ACC 4 VAC 1 (12" Hg) DRAIN VAC 1 (GRN) VAC 2 (BRN) SENSOR PRESS 1 (ORN) ACC 5 VAC 2 32062 (VAR.) DRAIN VAC 2 (BRN) − 2C - 4 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 124 2 WAY PINCH VALVE NORMALLY CLOSED 32063 LYSE LYSE SYRINGE SYRINGE AIR FLOW RESTRICTOR MOTOR-D MOTOR-C MOTOR-B CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW CLOSED MODE PROBE − 2C - 5 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 125 CLOSED MODE 32064 VAC 2 (BRN) OPEN MODE SAMPLE SENSOR ACC 5 CLOSED MODE SAMPLE VAC 2 OPEN/CLOSED MODE SAMPLE (VAR.) VACUUM #2 VAC 2 (BRN) − 2C - 6 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 126 WBC STAGING 2 WAY PINCH VALVE NORMALLY CLOSED NOC STAGING STAGING PUMP LINES AIR FLOW RESTRICTOR WC-1 MIXING CHAMBER VENTS CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW − 2C - 7 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 127 WBC STAGING 2 WAY PINCH VALVE NORMALLY CLOSED NOC STAGING STAGING PUMP LINES AIR FLOW RESTRICTOR WC-1 MIXING CHAMBER VENTS CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW − 2C - 8 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 128 FLOW CELL OUT 2 WAY PINCH VALVE NORMALLY CLOSED WASTE CHAMBER #1 VENT SHEATH FLOW AIR FLOW RESTRICTOR WC-1 32067 CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW − 2C - 9 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 129 2 WAY PINCH VALVE NORMALLY CLOSED WASTE CHAMBER #3 WASTE DRAIN WASTE CHAMBER #4 WASTE AIR FLOW RESTRICTOR MAIN WASTE OUT CHECK VALVE. ARROW SHOWS DIRECTION OF FLOW − 2C - 10 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 130 (12 psi) M. C. VENT MIXING CHAMBER FLOW CELL TO AIR MUFFLER TO AIR PUMP TO AIR 32069 WC-1 ACC 4 VAC 1 VENT (12" Hg) TRAP − 2C - 11 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 131 Type rl /nopri to reboot to Ready PEEK COMMAND (Use only if Analyzer was in the Ready POKE COMMAND Mode before entering DOS.) MOTOR CONTROL CALIBRATION F12/F1 accesses Dilution Factors − 2C - 12 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 132 PL-4 Flow Panel ......19 CELL-DYN 3200 System. It includes: PL-5 Vacuum/Pressure ......22 •...
  • Page 133 – Find the Tubing Designator on the tubing, e.g., small silicon tubing is designator S1. Click anywhere on the Tubing Map to jump to the Tubing Designator/Catalog Number table. – 3 - 2 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 134: Reagents And Supplies

    Reagents and Supplies Reagents and Supplies Item Part Number Description 99120-01 Calibrator, CELL-DYN 3000 93111-01 Ctrl, CELL-DYN 3000 (2.5 mL) 99129-01 Ctrl, CELL-DYN 3000 (3.0 mL) 03H78-01 Reagent, WBC Lyse (960 mL) 03H79-01 Reagent, DIL/STH (20 L) 03H80-01 Reagent, HGB/NOC (960 mL)

  • Page 135: Alphabetical Parts List Cross Reference

    PRE-J2 to MAM-J12 Bd, Sample Handler Module PL-18 PL-10 PL-12 Cable, 90 D PRE-J2 to MAM-J14 Bd, Sample Sensor#2 PL-9 Cable, CDM-J6 to FCM-J2 PL-16 Bd, SDM/CDM PL-15 – 3 - 4 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 136 Cable, MPM-J6 to CDB D-J1 PL-7 PL-11 Cable, FCM-J24 to VPM-J14 PL-14 Cable, MPM-J7 to CDB E-J1 PL-11 Cable, FCM-J25 to CPU-J7 PL-14 Cable, MPM-J7 to CDM-J1 PL-6 – 3 - 5 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 137 PL-1 PL-2 Cable, SVDM J4 to DC Motor PL-9 Cable, PSM (T)-J7 to XXXFAN PL-1 Cable, SVDM-J1 to Sensors PL-9 Cable, PSM (T)-J8 to XXXFAN PL-1 – 3 - 6 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 138 Cover, Left Front Door PL-19 Fitting, Reagent Inlet PL-3 Cover, Left Side PL-19 Fitting, Waste Outlet PL-3 Cover, Right Front Door PL-19 Fixture, Voltage Divider Rea/Sup – 3 - 7 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 139 Probe, Open Sample PL-6 Fuse, Fast 1A 250V 3 AG PL-2 Pump Assy, Peristaltic PL-8 Fuse, SB 15A 220/240VAC PL-1 Pump Head, Vac/Pres PL-5 ISE Cleaning Solutions Rea/Sup – 3 - 8 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 140 PL-17 Tubing Tyg 3/16” ID 5/16” OD Clr Specifications, Interface Rea/Sup Tubing Tyg 3/32” Switch, AC Input, 16A PL-17 Tubing Tyg O.125” ID 0.250” OD Gry – 3 - 9 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 141 Valve Assy, Solenoid, 3.9KG PL-5 Valve Assy, Solenoid, 5Kg PL-5 Valve Assy, Solenoid, NC PL-4 Valve, Check, Duckbill Valve, Rotary, 3-Port PL-8 Valve, Solenoid, 3-Way PL-18 Versalube™ Rea/Sup ® – 3 - 10 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 142: Power Supply Module

    6#0#3DUWV#/LVWV •# PL-1 Power Supply Module (1 of 2) PL-1 Power Supply Module (1 of 2) 13/14 ® – 3 - 11 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 143 8952089601 Cable, PSM (T)-J501 to PDM-J1 8240051601 Cord, Power 125Vac 10A 50065-01 Fuse, 8A 220/240 (<200VAC In) ≥ 61164-01 Fuse, 4A 220/240 ( 200VAC In) ® – 3 - 12 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 144: Power Distribution Module & Laser Supply

    6#0#3DUWV#/LVWV •# PL-2 Power Distribution Module & Laser Supply (1 of 3) PL-2 Power Distribution Module & Laser Supply (1 of 3) ® – 3 - 13 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 145 6#0#3DUWV#/LVWV •# PL-2 Power Distribution Module & Laser Supply (2 of 3) PL-2 Power Distribution Module & Laser Supply (2 of 3) ® – 3 - 14 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 146 Cable, PDM-J12 to SHM1-J12 8952089501 Cable, PDM-J13 to SHM2-J12 8960198001 Bd, PDM (Power Dist. Module) 8510019201 Fuse, Fast 1A 250V 3 AG 8921207601 Power Supply Laser ® – 3 - 15 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 147: Reagent Lines & Fans

    6#0#3DUWV#/LVWV •# PL-3 Reagent Lines & Fans (1 of 3) PL-3 Reagent Lines & Fans (1 of 3) ® – 3 - 16 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 148 6#0#3DUWV#/LVWV •# PL-3 Reagent Lines & Fans (2 of 3) PL-3 Reagent Lines & Fans (2 of 3) 3 2 0 1 7 ® – 3 - 17 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 149 Fan Guards & Filter, 3 Pcs 8260402701 Fan, 115VAC, 4.69” SQ 8952085701 Cable, CDM2-J7 to Ext Waste 8930038301 Fitting, Reagent Inlet 8930067601 Fitting, Waste Outlet ® – 3 - 18 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 150: Flow Panel

    6#0#3DUWV#/LVWV •# PL-4 Flow Panel (1 of 3) PL-4 Flow Panel (1 of 3) ® – 3 - 19 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 151 6#0#3DUWV#/LVWV •# PL-4 Flow Panel (2 of 3) PL-4 Flow Panel (2 of 3) 32019 ® – 3 - 20 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 152 Sensor, Overpressure 8921174802 Bottle, Waste (large) 8270721901 Filter, In-line, Sheath 8921210801 Chamber, Mixing, RBC/WBC 8952077801 Cable, HGB Flow Cell to FCM 8921217201 Bottle, Waste (small w/tubing) ® – 3 - 21 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 153 6#0#3DUWV#/LVWV •# PL-5 Vacuum/Pressure (1 of 2) PL-5 Vacuum/Pressure (1 of 2) ® – 3 - 22 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 154 Cable, MUX DAC CTRL Bus 8952084901 Cable, Serial Periph Bus 8952087901 Cable, VPM-J6 to CDM2-J4 8952083401 Cable, VPM-J18 to PRM-J1 8960122001 Bd, VPM (Vac/Pres Module) 8270040301 Filter, Pressure Pump ® – 3 - 23 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 155: Aspiration Tower & Open Probe Assemblies)

    6#0#3DUWV#/LVWV •# PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3) PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3) ® – 3 - 24 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 156 6#0#3DUWV#/LVWV •# PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3) PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3) ® – 3 - 25 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 157 Probe Driver Assy, Open 9960042001 Bd, Chopper Driver Module, CDB 8952084201 Cable, MPM-J7 to CDM-J1 8921076101 Motor, Stepper 8931201401 Block, Wash, Open Sample 8930094601 Probe, Open Sample ® – 3 - 26 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 158: Syringe Drivers (Ab, Cd)

    6#0#3DUWV#/LVWV •# PL-7 Syringe Drivers (AB, CD) (1 of 2) PL-7 Syringe Drivers (AB, CD) (1 of 2) ® – 3 - 27 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 159 Bd, Chopper Driver Module, CDB 8952015501 Cable, MPM-J5 to CDB C-J1 8921217401 Syringe, Driver Assy, (CD) 02H82-01 Syringe, Diluent (10 mL) 8952084501 Cable, MPM-J3 to CDB A-J1 ® – 3 - 28 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 160: Peri-Pump, Sample Handler Module #1, & Open Probe Assembly

    PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2) PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2) ® – 3 - 29 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 161 8921206101 Driver Assy, Y-Valve, Complete 8921207701 Motor, Y-Valve 8370830101 Sensor, Photodiode & LED 8952087101 Cable, Y-Valve Sensor 8250900301 Valve, Rotary, 3-Port 8370181801 Sensor, Sample, Ultrasonic ® – 3 - 30 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 162: Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards

    PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (1 of 3) PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (1 of 3) ® – 3 - 31 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 163 PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (2 of 3) PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (2 of 3) P/O Bd. ® – 3 - 32 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 164 Cable, MDM-J2 to SDM4-J7 8952079501 Cable, MDM-J1 to SDM4-J8 8952087101 Cable, Y-Valve Sensor 8370181801 Bd, Ultrasonic Sensor 8960116701 Bd, Sample Sensor#2 8952082001 Cable, SS2-J1 to FCM-J14 ® – 3 - 33 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 165: Laser Bench

    6#0#3DUWV#/LVWV •# PL-10 Laser Bench (1 of 2) PL-10 Laser Bench (1 of 2) ® – 3 - 34 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 166 Bd, HV/Preamp 8921142901 Cable, PMT Socket 8952085501 Cable, 90 PRE-J2 to MAM-J12 8952085601 Cable, 90 D PRE-J2 to MAM-J14 8921145201 Flow Cell, Optical 8921181501 Mirror Perforated ® – 3 - 35 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 167: Motor Processor Module

    6#0#3DUWV#/LVWV •# PL-11 Motor Processor Module (1 of 2) PL-11 Motor Processor Module (1 of 2) ® – 3 - 36 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 168 Cable, MPM-J4 to CDB B-J1 8952015501 Cable, MPM-J5 to CDB C-J1 8952015401 Cable, MPM-J6 to CDB D-J1 8952084201 Cable, MPM-J7 to CDB E-J1 8952056301 Cable, MPM-J9 to CDB G-J1 ® – 3 - 37 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 169: Main Amplifier & Signal Processor Modules

    6#0#3DUWV#/LVWV •# PL-12 Main Amplifier & Signal Processor Modules (1 of 2) PL-12 Main Amplifier & Signal Processor Modules (1 of 2) ® – 3 - 38 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 170 Cable, CPU-J10 to SPM-J5 8952084601 Cable, External CPU Bus 8952085401 Cable, MAM-J12 to PD PRE 8952085301 Cable, MAM-J10 to PD PRE 8952050601 Cable, MAM-J4 to CPU-J9 ® – 3 - 39 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 171: Central Processing Unit/Device Control Module

    6#0#3DUWV#/LVWV •# PL-13 Central Processing Unit/Device Control Module (1 of 2) PL-13 Central Processing Unit/Device Control Module (1 of 2) ® – 3 - 40 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 172 8952085001 Cable, CPU-J12 to SPM-J7 8952050601 Cable, MAM-J4 to CPU-J9 8952085201 Cable, CPU-J7 to FCM-J25 8952050501 Cable, CPU-J10 to SPM-J5 8952085101 Cable, CPU-J8 to FCM-J22 ® – 3 - 41 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 173: Flow Control Module, View #1

    6#0#3DUWV#/LVWV •# PL-14 Flow Control Module, View #1 (1 of 3) PL-14 Flow Control Module, View #1 (1 of 3) ® – 3 - 42 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 174 6#0#3DUWV#/LVWV •# PL-14 Flow Control Module, View #1 (2 of 3) PL-14 Flow Control Module, View #1 (2 of 3) ® – 3 - 43 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 175 Cable, SS2-J1 to FCM-J14 Cable, P/O Ultrasonic Sen 8952083901 Cable, FCM-J10 to DIL OP Sen 8952078301 Cable, FCM-J9 to Start SW 8952086201 Cable, FCM-J8 to SVDM-J2 ® – 3 - 44 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 176 PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (1 of 3) PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable Distribution Module (1 of 3) MDM-J1 MDM-J2 V32 V31 ® – 3 - 45 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 177: Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable Distribution Module

    PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (2 of 3) PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable Distribution Module (2 of 3) V27 V28 ® – 3 - 46 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 178 Cable, PSM (Back)-J2 to CDM1-J3 8952083001 Cbl, SDM/CDM-J1 to SDM1-J10 8952085801 Cbl, SDM/CDM-J2 to SDM2-J10 8960093001 Bd, Solenoid Driver Module, SDM 8952083701 Cable, SDM Data/+5V Bus ® – 3 - 47 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 179: Solenoid Driver Modules 5,6,9 & Cable

    PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3) PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3) ® – 3 - 48 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 180 PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3) PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3) V92 V91 ® – 3 - 49 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 181 8952086001 Cable, SDM5-J10 to CDM2-J2 8952086601 Cable, DCM2-J3 to PSM(B)-J1 8952087901 Cable, CDM2-J4 to VPM-J6 8952083701 Cable, SDM Data/+5V Bus 8952086101 Cable, SDM9-J10 to FCM-J4 ® – 3 - 50 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 182: Main Computer Boards & Computer Assemblies

    6#0#3DUWV#/LVWV •# PL-17 Main Computer Boards & Computer Assemblies (1 of 4) PL-17 Main Computer Boards & Computer Assemblies (1 of 4) ® – 3 - 51 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 183 6#0#3DUWV#/LVWV •# PL-17 Main Computer Boards & Computer Assemblies (2 of 4) PL-17 Main Computer Boards & Computer Assemblies (2 of 4) ® – 3 - 52 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 184 6#0#3DUWV#/LVWV •# PL-17 Main Computer Boards & Computer Assemblies (3 of 4) PL-17 Main Computer Boards & Computer Assemblies (3 of 4) ® – 3 - 53 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 185 Cable Kit, Internal I/O 06H74-01 Keyboard, PCAT 06H75-01 Cover, Keyboard 04H44-01 Monitor, 14” VGA Color 8510751801 Switch, AC Input, 16A 8952087017 Cable, Interface, HSSL 20005-01 Cable, Printer, Parallel ® – 3 - 54 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 186: Sample Loader

    6#0#3DUWV#/LVWV •# PL-18 Sample Loader (1 of 5) PL-18 Sample Loader (1 of 5) 3 2 0 7 0 ® – 3 - 55 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 187 6#0#3DUWV#/LVWV •# PL-18 Sample Loader (2 of 5) PL-18 Sample Loader (2 of 5) 3 2 0 7 1 ® – 3 - 56 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 188 6#0#3DUWV#/LVWV •# PL-18 Sample Loader (3 of 5) PL-18 Sample Loader (3 of 5) 3 2 0 7 2 ® – 3 - 57 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 189 6#0#3DUWV#/LVWV •# PL-18 Sample Loader (4 of 5) PL-18 Sample Loader (4 of 5) 3 2 0 7 3 ® – 3 - 58 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 190 Cylinder, Air, Mixer Lift 8510510101 Switch, Reed, w/Cable 8450876601 Valve, Solenoid, 3-Way 8310842201 Regulator, Pressure 8280861301 Cylinder, Air, X & Y Index 8932074001 Shield, SHM #1, 2 – 3 - 59 CELL-DYN® 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 191: Covers

    6#0#3DUWV#/LVWV •# PL-19 Covers (1 of 3) PL-19 Covers (1 of 3) 3 2 0 7 4 ® – 3 - 60 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 192 6#0#3DUWV#/LVWV •# PL-19 Covers (2 of 3) PL-19 Covers (2 of 3) 3 2 0 7 5 ® – 3 - 61 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 193 Sensor, Photodiode & LED 8952078001 Cable, Nose Cover Sensor 8932062101 Cover, Left Front Door 8510810901 Switch, Touch Bar 8952083801 Cable, FCM-J7 to SAB-J1 8960192001 Bd, Status Alert ® – 3 - 62 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 194: Flow Panel

    6#0#3DUWV#/LVWV •# Flow Panel Flow Panel ® – 3 - 63 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 195: Pneumatic Unit

    6#0#3DUWV#/LVWV •# Pneumatic Unit Pneumatic Unit ® – 3 - 64 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 196: Sample Loader

    6#0#3DUWV#/LVWV •# Sample Loader Sample Loader ® – 3 - 65 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 197: Tubing Designator/Catalog Number Table

    Fitting T 0.125" X 0.125" X 0.125" 31082-01 Fitting (T4) 8310809501 Fitting T 0.062" X 0.125" X 0.062" 8310800701 Fitting T 0.093 X 0.093 X 0.093 ® – 3 - 66 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 198 0.125" ID 0.250" 0D Pur ® Y638 8310663801 Tubing Tygon O.125” ID 0.250” OD Gry ® 8310653501 Tubing Tygon 3/32” Y FIT 8310810801 Fitting 0.062” X 0.062” X 0.062” ® – 3 - 67 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 199 Syringe Driver Assembly (RR-11) ..... .69 ® 4 - 1 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 -- August 1998...

  • Page 200: Shear Valve & Shear Valve Driver (Rr-1)

    1. Ensure that the shear valve is in the cleaning position using the special protocols function. 2. With analyzer power off, open the left and right access doors and remove the safety cover. − ® 4 - 2 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 201 • Shear Valve & Shear Valve Driver (RR-1) Figure 4-2: Shear Valve Retaining Screw 3. Remove the shear valve retaining screw from the center of the shear valve. − ® 4 - 3 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 202 4. Carefully remove the three ceramic pieces from the shear valve. Set the fragile center piece aside. Do not disconnect tubing from the other two pieces. − ® 4 - 4 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 203 Figure 4-4: Removing Top Cover and Optics Cover 5. Remove the top cover (4 screws) and the optics cover (two 6-32 screws and two 8-32 screws.) − ® 4 - 5 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 204 • Shear Valve & Shear Valve Driver (RR-1) Figure 4-5: Shear Valve Driver MountingScrews 6. Remove the 3 screws that hold the assembly in place. − ® 4 - 6 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 205 Shear Valve Cables J2 and J3. 7. Move the preamplifier cables out of the way and disconnect cables at J2 and J3 from the Driver Bd. − ® 4 - 7 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 206 8. Angle the assembly and remove from the back Replacement Procedure Replace by following the steps above in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 8 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 207: Y-Valve (Rr-2)

    Y-Valve (RR-2) Figure 4-8: Front Access Doors Removal Procedure 1. With analyzer power off, open the left and right access doors and remove the front cover. − ® 4 - 9 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 208 7#5HPRYDO#)#5HSODFHPHQW# • Y-Valve (RR-2) Figure 4-9: Y-Valve Tie Wrap 2. Locate the Y-valve assembly and cut the tie wrap on the lower left-hand corner. − ® 4 - 10 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 209 7#5HPRYDO#)#5HSODFHPHQW# • Y-Valve (RR-2) Figure 4-10: Y-Valve Block Tubing 3. Disconnect the tubing from the three ports on the valve block − ® 4 - 11 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 210 Figure 4-11: Y-Valve Mounting Screws 4. Remove the two screws at the top near the motor and cut the tie wrap to the left of the wash block. − ® 4 - 12 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 211 5. Disconnect the two sensor connections from the base of the assembly. NOTE: For replacement purposes, note which socket each comes from as they are not identified. − ® 4 - 13 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 212 7#5HPRYDO#)#5HSODFHPHQW# • Y-Valve (RR-2) Figure 4-13: Top Cover Removal 6. Remove the top cover (4 screws). − ® 4 - 14 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 213 Feed cable through front panel and remove assembly. Replacement Procedure Follow the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 15 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 214: Ultrasonic Sensor (Rr-3)

    Ultrasonic Sensor (RR-3) Figure 4-15: Front Access Doors Removal Procedure 1. With analyzer power off, open the left and right access doors and remove the front cover. − ® 4 - 16 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 215 7#5HPRYDO#)#5HSODFHPHQW# • Ultrasonic Sensor (RR-3) Figure 4-16: Top Cover Removal 2. Remove the top cover (4 screws.) − ® 4 - 17 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 216 7#5HPRYDO#)#5HSODFHPHQW# • Ultrasonic Sensor (RR-3) Figure 4-17: Y-Valve Tie Wrap 3. Locate the ultrasonic sensor and cut the tie wrap on the Y-valve − ® 4 - 18 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 217 • Ultrasonic Sensor (RR-3) Figure 4-18: Ultrasonic Sensor Fluid Connections 4. Remove the two fluid connections from the left and right of the ultrasonic sensor. − ® 4 - 19 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 218 • Ultrasonic Sensor (RR-3) Figure 4-19: Ultrasonic Sensor Holding Screws 5. Remove the two screws on the front panel which hold the Sensor Bd. bracket. − ® 4 - 20 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 219 Sensor Bd and feed connector through front panel. Replacement Procedure Follow the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 21 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 220: Open Probe Drive Assembly (Rr-4)

    Avoid contact with the tips of the needle/probe. 1. With analyzer power off, open the left and right access doors and remove the front cover. − ® 4 - 22 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 221 Figure 4-22: Wash Block Holding Screws 2. Locate and remove the two screws holding the wash block to the open probe assembly and let the wash block dangle. − ® 4 - 23 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 222 • Open Probe Drive Assembly (RR-4) Figure 4-23: Open Probe Assembly Mounting Screws 3. Remove the 4 screws holding the assembly to the front panel. − ® 4 - 24 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 223 7#5HPRYDO#)#5HSODFHPHQW# • Open Probe Drive Assembly (RR-4) Figure 4-24: Top Cover Removal 4. Remove the top cover (4 screws). − ® 4 - 25 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 224 7#5HPRYDO#)#5HSODFHPHQW# • Open Probe Drive Assembly (RR-4) Figure 4-25: Right Side Cover Removal 5. Remove the right side cover (4 screws). − ® 4 - 26 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 225 Figure 4-26: CPU Door Access 6. Loosen the upper and lower thumbscrews and open the CPU door assembly to gain access to the back of the aspiration assembly. − ® 4 - 27 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 226 Chopper Driver Bd. and remove the Probe Assembly. Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 28 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 227: Optics Bench (Rr-5)

    Do not touch the male pins. Short male pins to Chassis. 1. With the analyzer power off, remove the top cover (4 screws) and the optics cover (4 screws). − ® 4 - 29 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 228 Figure 4-29: Optics Bench Mounting Screws 2. Remove the five optics mounting screws. Remove screws from the aluminum plate, not the black base of the Optics Bench. − ® 4 - 30 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 229 • Optics Bench (RR-5) Figure 4-30: Optics Signal Cables 3. Remove the 4 signal cables from the optics bench. Lay the bundle away from the optics. − ® 4 - 31 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 230 Figure 4-31: Flow Cell Panel 4. Remove the two mixing chambers. Do not disconnect the tubing. Loosen the two flow cell panel thumbscrews and open the panel. − ® 4 - 32 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 231 7#5HPRYDO#)#5HSODFHPHQW# • Optics Bench (RR-5) Figure 4-32: Flow Cell Tubing 5. Disconnect the flow cell tubing (1 - 5) from the manifold. − ® 4 - 33 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 232 Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 34 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 233: Power Supply (Rr-6)

    Note 1 : Five-pin connections J1, J2, and J3 are the same. Note 2 : Three-pin connection is empty directly above an two-pin connection. − ® 4 - 35 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 234 7#5HPRYDO#)#5HSODFHPHQW# • Power Supply (RR-6) Figure 4-35: Power Supply Mounting Screws 3. Remove power supply mounting screws (6 places). − ® 4 - 36 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 235 7#5HPRYDO#)#5HSODFHPHQW# • Power Supply (RR-6) Figure 4-36: Power Supply Removal 4. Lift power supply up and out. − ® 4 - 37 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 236 2. Ensure fuse is present and OK. 3. To reinstall, follow the removal steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 38 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 237: Vacuum/Pressure Assembly (Rr-7)

    NOTE: It is no longer necessary to remove the Vacuum/Pressure Assembly when servicing the VPM, pumps, etc. The Assembly is mounted on movable slides in 3200 analyzers built after May, 1998. To access the Assembly: 1. With the analyzer power off, remove the left side panel (4 screws).
  • Page 238 7#5HPRYDO#)#5HSODFHPHQW# • Vacuum/Pressure Assembly (RR-7) Figure 4-39: Fan Assembly Removal 2. Disconnect the fan power cord and remove fan assembly (4 screws). − ® 4 - 40 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 239 7#5HPRYDO#)#5HSODFHPHQW# • Vacuum/Pressure Assembly (RR-7) Figure 4-40: Vacuum/Pressure Assembly Removal 3. Remove the locking screw and pull vacuum/pressure assembly out until unit locks in place. − ® 4 - 41 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 240 Figure 4-41: Vacuum/Pressure Assembly Return 1. To return assembly to normal position, push up on clip and push assembly back in place. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 42 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 241: Tower Assembly (Rr-8)

    Avoid contact with the tips of the needle/probe. 1. With the analyzer power off, open the left and right access doors and remove the safety cover. − ® 4 - 43 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 242 7#5HPRYDO#)#5HSODFHPHQW# • Tower Assembly (RR-8) Figure 4-43: Sample Loader Panels 1. Remove the left and right panels from the rear rail of the sample loader. − ® 4 - 44 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 243 7#5HPRYDO#)#5HSODFHPHQW# • Tower Assembly (RR-8) Figure 4-44: Tower Assembly Screw 2. Remove the screw at the top of the tower assembly. − ® 4 - 45 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 244 • Tower Assembly (RR-8) Figure 4-45: Sample Loader End Screws 3. Loosen the two screws at each end of the sample loader just one turn. − ® 4 - 46 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 245 7#5HPRYDO#)#5HSODFHPHQW# • Tower Assembly (RR-8) Figure 4-46: Slide Sample Loader Forward 4. Lift sample loader and slide forward until it stops (about 2”). − ® 4 - 47 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 246 • Tower Assembly (RR-8) Figure 4-47: Tower Cable Tie Wraps 5. Cut the two tie wraps around cable bundle in front of flow cell panel. − ® 4 - 48 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 247 • Tower Assembly (RR-8) Figure 4-48: Tower Assembly Cables 6. Disconnect J1, J8, J10 and J13 from the SHM Bd mounted to the flow cell panel. − ® 4 - 49 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 248 Disconnect the tubing at aspiration probe and wash block b. Remove the glass bubble trap (2 screws). Do not disconnect tubing. c. Lift the tubing from under the clip. − ® 4 - 50 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 249 Tower Assembly and lift the assembly out. NOTE: Long screw at base. Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 51 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 250: Hgb Flow Cell (Rr-9)

    HGB Flow Cell (RR-9) Figure 4-51: Front Access Doors Removal Procedure 1. With analyzer power off, open left and right access doors and remove front cover. − ® 4 - 52 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 251 • HGB Flow Cell (RR-9) Figure 4-52: HGB Flow Cell Tubing 2. Remove tubing from the flow cell: two in front, one on top and one at the base. − ® 4 - 53 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 252 7#5HPRYDO#)#5HSODFHPHQW# • HGB Flow Cell (RR-9) Figure 4-53: HGB Flow Cell Electrical Connection 3. Disconnect the inline electrical connection. − ® 4 - 54 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 253 Figure 4-54: HGB Flow Cell Cover Screws 4. Remove the two screws on top of the HGB flow cell to remove the flow cell cover. − ® 4 - 55 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 254 5. Remove the two screws at back of the flow cell to remove it. Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 56 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 255 • Sample Loader (RR-10) Sample Loader (RR-10) Figure 4-56: Front Access Doors Removal Procedure Cell-Dyn 3200 Analyzers built after May 1998 do not require sample loader removal to access front panel. Follow Part A to access front panel. Follow Parts A and B to remove and replace the loader.
  • Page 256 7#5HPRYDO#)#5HSODFHPHQW# • Sample Loader (RR-10) Figure 4-57: Sample Loader Panels 2. Remove the left and right panels from the rear rail of the sample loader. − ® 4 - 58 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 257 7#5HPRYDO#)#5HSODFHPHQW# • Sample Loader (RR-10) Figure 4-58: Tower Assembly Screw 3. Remove the screw at the top of the tower assembly. − ® 4 - 59 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 258 • Sample Loader (RR-10) Figure 4-59: Sample Loader End Screws 4. Loosen the two screws at each end of the sample loader just one turn. − ® 4 - 60 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 259 7#5HPRYDO#)#5HSODFHPHQW# • Sample Loader (RR-10) Figure 4-60: Slide Sample Loader Forward 5. Lift sample loader and slide forward until it stops (about 2”). − ® 4 - 61 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 260 Figure 4-61: Tower Assembly Base Screws 1. Remove the three hex head screws (7/64” allen) from the base of the tower assembly. NOTE: Larger screw at base. − ® 4 - 62 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 261 2. Release the clip around the tower assembly tubing. Disconnect the two tubes as shown in the inset. Lay the assembly on top of the instrument. − ® 4 - 63 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 262 7#5HPRYDO#)#5HSODFHPHQW# • Sample Loader (RR-10) Figure 4-63: Vacuum & Pressure Connections 3. Disconnect the vacuum and pressure connections by pressing on the metal clip. − ® 4 - 64 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 263 Figure 4-64: SHM Cables 4. Remove plastic cover from SHM. Disconnect all cables from SHM. Remove cable clamp (1 screw). NOTE: Cables are marked for proper reassembly. − ® 4 - 65 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 264 7#5HPRYDO#)#5HSODFHPHQW# • Sample Loader (RR-10) Figure 4-65: Remove Cable Clamps 5. Remove cable clamps. Set aside ribbon cable (J16) and multicolored cable (J12). − ® 4 - 66 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 265 Figure 4-66: Sample Loader Cover Removal 6. Remove loader surround cover by loosening front three screws and removing left and right end screws (2 at each end). − ® 4 - 67 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 266 Lift loader from brackets. Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 68 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 267 Figure 4-68: Front Access Doors (RR-11) Removal Procedure 1. With analyzer power off, open the left and right access doors and remove the safety cover. − ® 4 - 69 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 268 7#5HPRYDO#)#5HSODFHPHQW# • Syringe Driver Assembly (RR-11) Figure 4-69: Sample Loader Right Panel 2. Remove the right panel from the rear rail of the sample loader. − ® 4 - 70 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 269 7#5HPRYDO#)#5HSODFHPHQW# • Syringe Driver Assembly (RR-11) Figure 4-70: Tower Assembly Screw 3. Remove the screw at the top of the tower assembly. − ® 4 - 71 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 270 • Syringe Driver Assembly (RR-11) Figure 4-71: Sample Loader End Screws 4. Loosen the two screws at each end of the sample loader just one turn. − ® 4 - 72 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 271 7#5HPRYDO#)#5HSODFHPHQW# • Syringe Driver Assembly (RR-11) Figure 4-72: Slide Sample Loader Forward 5. Lift sample loader and slide forward until it stops (about 2”). − ® 4 - 73 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 272 7#5HPRYDO#)#5HSODFHPHQW# • Syringe Driver Assembly (RR-11) Figure 4-73: Luer Fittings 6. Unscrew the luer fittings from top of each syringe. − ® 4 - 74 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 273 (Right side mounting screws shown here.) Then remove the spacer bar (two screws) between the two syringe driver assemblies. − ® 4 - 75 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 274 Replacement Procedure Replace by following the above steps in reverse order. Verification Procedure See Verification Matrix, Section 5.2. − ® 4 - 76 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 275 VP-49 HGB Flow Cell Cleaning Procedure ..48 VP-21 Optical Flow Cell Coarse Alignment ..21 ® − 5 - 1 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 276 CD3200 Analyzer and Autoloader – A list of conditions indicating the need to perform a particular procedure – Required tools and materials for each procedure ® − 5 - 2 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 277: Sample Loader (Rr-10)

    VP-6, VP-7, VP-56, VP-57, VP-9 Syringe Driver Assembly SDM/CDM VP-58 Sample Handler Module #1 VP-31, VP-32, VP-33, VP-34 VP-6, VP-8, VP-7, VP-56, Peri-Pump Assembly VP-57, VP-58 ® − 5 - 3 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 278: How To Use This Chapter

    ASSOCIATED VPs REPLACED ASSEMBLY (Perform In Order Listed) S. Valve Center Ceramic VP-1, VP-56, VP-57, VP-58 Hard Disk Drive VP-53, VP-54 Floppy Disk Drive VP-55 ® − 5 - 4 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 279: Verification Procedures

    5-1. Connect voltmeter ground lead to TP4 +24 VDC REG 24.0-25.0 <75MV PP on power supply module. 3. Verify the voltages according to Table 5-1. ® − 5 - 5 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 280: A/D Verification/10 Volt Reference Adjustment

    # 2 Phillips Screwdriver Adjustment • Digital Voltmeter The CELL-DYN 3200 utilizes an A/D converter to convert all critical voltages levels to a 12-bit digital code. This code is read • Clip Leads by the computer and these voltages are then displayed on the •...

  • Page 281: Mam Reference Voltage Verification/Adjustment

    3. If necessary, adjust R16 for a reading of 10.00 volts +/- 0.01V. • # 2 Phillips Screwdriver • Digital Voltmeter • Clip Leads • Potentiometer Adjustment Tool or Small Slotted Screwdriver ® − 5 - 7 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 282: Hgb Current Verification/Adjustment

    „ MOTOR OPERATION to the first diagnostic screen, and press 2. Press „ F12 „ MOTOR PWR CHECKING „ F1 and wait for the display. ® − 5 - 8 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 283: Motor Operation Verification

    „ DIAGNOSTICS „ ENTER „ MORE 3. Verify that the assembly moves smoothly without stopping „ MOTOR OPERATION or jerking. ® − 5 - 9 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 284: Solenoid Bank Verification

    Required Tools & Materials: 2. Record the tube bar code number, and place tube at the • None read station. 3. Press „ BAR CODE ALIGNMENT ® − 5 - 10 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 285: Specimen Sensor #2 Verification/Adjustment

    3. Create a specimen dilution using LOW ABNORMAL Verification Procedure: CONTROL material and CELL-DYN 3200 Diluent/Sheath 1. Turn power off, install loopback connector at COM1 (top that gives an RBC value of 0.8MEG/ul, +/- .03. serial connector) on PC module, and power-up and 4.

  • Page 286: Mam & Spm Signal Generator Verification

    „ TRANSMIT MESSAGE Required Tools & Materials: and verify that the message received matches the • None message sent: “CELL-DYN serial interface test.” 4. Press Verification Procedure: „ STOP TRANSMISS 1. Ensure that the instrument is in the “READY” mode.

  • Page 287: Vacuum & Pressure Level Verification/Adjustment

    5 through 8. a gross leak in the system. 5. Press „ MORE ® − 5 - 13 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 288: Laser Replacement/Alignment Procedure

    .050 Allen Wrench splitter/polarizer assembly, and remove assembly from • Optics Alignment Kit Cat. #8921183801 path. • DVM with Clip Leads • Laser Power Meter ® − 5 - 14 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 289 This may have to be 23. Adjust the Y alignment screw on the rear mirror until the red images are in the center 1/3 of the 90 slit. ® − 5 - 15 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 290: Front/Rear Mirror Replacement/Alignment Procedure

    • DVM with Clip Leads manifold. • Laser Power Meter Verification Procedure: 1. Remove top cover, both optics covers, and open flow cell access door. ® − 5 - 16 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 291: Optical Flow Cell Verification/Fine Alignment

    FL O W C E LL M A NIFO L D The alignment of the optical flow cell is one of the most critical alignments performed on the CELL-DYN 3200. It must be optimized so that the instrument can properly perform the WBC 5-Part Differential measurements.
  • Page 292 6. Open the flow cell access door, located at the top left of optical bench, since once properly aligned and secured, the Analyzer. none of the assemblies should move under normal conditions. ® − 5 - 18 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 293 19. Perform WBC OPTI-CAL Verification/Adjustment (VP-24). 20. Perform NOC Gain Adjustment VP-25. 21. Perform RBC/PLT 0 Gain Verification/Adjustment VP-26. 22. Perform RBC/PLT 10 Gain Verification/Adjustment VP-27. ® − 5 - 19 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 294 8#0#9HULILFDWLRQ#3URFHGXUHV • VP-20 Optical Flow Cell Verification/Fine Alignment Figure 5-4 CELL-DYN 3200 Optics Bench R ear M irror X A xis Laser S trap Laser S trap 5 M illiw att H E N E Laser Vertical P olarization 632.8 N anom eters .75"...

  • Page 295: Optical Flow Cell Coarse Alignment

    CCW until the pattern of the rear wall of the flow cell 9. Press channel is observed, and record the micrometer setting „ CALC CV (Figure 5-5-B). ® − 5 - 21 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 296: Pmt Dynode Voltage Verification/Adjustment

    Place 2 mL of diluent in a clean container. „ F12 µ b. Add 15 drops of 7.00 beads and mix well. „ F1 „ SET POINT ENTRY ® − 5 - 22 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 297: Wbc Gain Verification/Adjustment

    13. Repeat steps 11 and 12 for the 90 depolarized preamplifier (right PCB). Required Tools & Materials: µ • 7.00 Diameter Beads • CELL-DYN 3200 Diluent/Sheath ® − 5 - 23 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 298: Wbc Opti-Cal Verification/Adjustment

    It also determines and stores the Beads 7. Press reference channels to be used for reference after an OPTI-CAL „ RETURN gain adjustment. to access “AUTO GAIN ADJUSTMNT ® − 5 - 24 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 299 „ F12 Z = Current Mean Channel from Step 14. „ F1 17. Press „ RETURN 8. Press „ BEADS/BLOOD COMPARE „ SET POINT ENTRY ® − 5 - 25 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 300: Noc Gain Adjustment

    NOTE: These Beads Channel locations are now specific • None to this analyzer and must be entered into the AUTO GAIN table. 25. Press „ RETURN „ RETURN ® − 5 - 26 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 301: Rbc/Plt 0° Gain Verification/Adjustment

    Add 15 drops of 7.00 beads to a vacutainer. „ ENTER c. Add 2 ml of 3200 Diluent/Sheath reagent. 7. If necessary, move the cursor to the NOC Lo threshold, 2. From Main Menu, press change it to 400, and press „...

  • Page 302: Rbc/Plt 10° Gain Verification/Adjustment

    Add 10 drops of Beads and 20 drops of diluent to a vacutainer and mix well. and press the “START SWITCH” to aspirate Beads and run a count cycle. ® − 5 - 28 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 303: Mam 0° & 10° Log Amplifier Verification

    7. Press calculating the ratio of the peak channels of the three sizes. „ RETURN The CELL-DYN 3200 uses the 0 channel for and MCV, and the to access “AUTO GAIN ADJUSTMNT.” channel for RBCs, PLTs, and MPV, with the log amplifiers µ...
  • Page 304 P.C. P.C. µ b. Add 15 drops of 7.00 beads to a vacutainer. 3.29 Set at 120 c. Add 2 ml of 3200 Diluent/Sheath reagent. Falls at 4.99 2. In the “RUN” menu, press Set at „ SPECIMEN TYPE 7.00 Falls at 200 „...
  • Page 305 PLT 10 peak channel number, and skip step 14. 14. Perform RBC/PLT 10 Gain Verification/Adjustment (VP-27), and repeat steps 12 and 13. ® − 5 - 31 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 306: 0° Mam Log Amplifier Verification/Adjustmen

    0.2mv. 3 2 0 80 B lack (G N D ) 4. Move the positive lead to TP11, and adjust R102 for -23.0mv +/- 0.2mv ® − 5 - 32 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 307 7. Leave meter as is, and adjust R132 for a reading of 1.72V +/- 0.010V. NOTE: After performing this procedure the 0 gain must be verified/adjusted with Beads (VP-26). ® − 5 - 33 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 308: 10° Mam Log Amplifier Verification/Adjustment

    Also verify that the GS1 sensor status displays the following: 2. Attach the positive lead of the DVM to TP17 and the negative lead to TP7. GS1 UP:SENSOR STATUS ® − 5 - 34 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 309: Gs2 Stop Solenoid Verification

    (1=sensor blocked, 0=sensor unblocked). It also displays the type of tube sensed at the aspirate position: BD, SST, or No Tube. ® − 5 - 35 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 310: Mixer Up/Down Verification

    Sarstedt Tube Example: VP-36 Mixer Head Rotation Verification GS1 UP: Sensor Status Required Tools & Materials: GS1=1 S1=0 S2=0 • None GS1 Down: Sensor Status Tube Type ® − 5 - 36 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 311: Mixer Bladders Verification

    3. Alternately press “GRIP TUBE” and “RELEASE TUBE” and verify that the mixer bladders inflate and deflate completely, and that there are no pressure or vacuum leaks. ® − 5 - 37 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 312: Cross Transfer Arms & Rack Sensors Verification

    EXTEND ARMS:LOAD SENSOR = 0 3. Verify that the bar code number on the screen matches the UNLOAD FULL number on the rack. UNLOAD WARN = 0 ® − 5 - 38 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 313: Mixer Bladders Pressure Verification/Adjustment

    „ CYCLE BLADDERS 5. Remove tubing from vacuum/pressure port on mixer head, and connect tubing to pressure gauge. 6. Press „ GRIP TUBE to apply pressure. ® − 5 - 39 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 314: Optics Bench Cleaning Procedure

    • High Grade Lens Paper (Kodak recommended - DO NOT USE Baxter S/P, as it may scratch the lens.) • Canned Air - Optics Grade ® − 5 - 40 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 315 Figure 5-10 Laser Output Halo 3 2 0 84 Laser Tube R ear M irror N O R ed H alo 3 2 0 83 ® − 5 - 41 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 316 12. Clean 10 mirror. a. Mark position of mirror and housing with pencil. Refer to Figure 5-13. R em ove M irror 3 2 0 88 ® − 5 - 42 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 317: Optical Flow Cell Cleaning & De-Bubble Procedure

    Tighten mirror screws ensuring maximum reading is n EMPTY FLOW CELL maintained, and erase pencil marks. 14. Perform WBC Gain Verification/Adjustment (VP-23). 15. Perform NOC Gain Adjustment (VP-25). ® − 5 - 43 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...
  • Page 318 11. Refer to Figure 5-15. Verify that there are no large bubbles in the flow cell. If large bubbles are present, perform Optical Flow Cell Wetting Procedure (VP-43). ® − 5 - 44 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 319: Optical Flow Cell Wetting Procedure

    5. Refer to Figure 5-15. Remove elbow from right side of S he a th In 3 2 0 90 solenoid 57, and connect elbow to syringe. S am p le In ® − 5 - 45 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 320: Laser Power Output Verification

    Power meter should read at least 1/5 of that obtained c. If power is less than 5 mW, replace laser tube. in step 3. 5. Perform Optical Offsets Verification (VP-47). ® − 5 - 46 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 321: Optical Offsets Verification

    4. Use damp lint free wipes to clean front and rear surfaces 2. Ensure top cover is in place, or cover optics bench with of front and rear ceramic sections. black cloth. ® − 5 - 47 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 322: Hgb Flow Cell Cleaning Procedure

    µ ≤ µ 0.10 K/ L, RBC 0.02 M/ , and PLT 5 K/ 5. Manually open solenoid 93 to drain liquid from flow cell. ® − 5 - 48 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 323: Open Mode Aspiration Path Cleaning Procedure

    4. Place a tissue under probe, run stylet into the top of probe until it extends out the bottom, and wipe any material from „ VOLTAGE READINGS stylet. Run up and down several times and remove. ® − 5 - 49 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 324: Closed Mode Aspiration Path Cleaning Procedure

    Verification Procedure: 1. In the “RUN” mode use “CHANGE SAMPLER” to select “CLOSED SAMPLER.” 2. From Main Menu, press „ DIAGNOSTICS „ MORE „ TOWER TEST ® − 5 - 50 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 325: Power-Up Cpu/Dcm 7-Segment Led Verification

    VP-52 Power-Up CPU/DCM 7-Segment LED VP-53 Software Installation Procedure Verification Required Tools & Materials: Required Tools & Materials: • CELL-DYN System Installation Disks 1 & 2 P.N. 9380590 • None Verification Procedure: Verification Procedure: 1. Turn system power off. 1. Power-up the system and observe the 7-segment LED on 2.

  • Page 326: Floppy Drive Verification

    “MAIN” menu. 6. If there are no indications of a defect, type rll and press “ENTER” to return to the “MAIN” menu. ® − 5 - 52 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 327: Background Counts Verification

    5. Verify the CVs are within specification according to the 3. Verify that the background counts are within specifications following table. according to the following table. ® − 5 - 53 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 328: System Calibration Verification/Adjustment

    NOTE: If any problems are encountered, refer to “Auto-Cal Procedure-Open Mode” in Chapter 6 of the • #2 Phillips Screwdriver CELL-DYN 3200 Operations Manual for a complete description of the calibration procedure. a. Ensure Analyzer in Open Mode. ® −...

  • Page 329: Vacuum Accumulator #2 Rinsing Procedure

    #2 Phillips Screwdriver Verification Procedure 1. Initialize Analyzer, and remove Left Side Cover. Figure 5-23 Vacuum/Pressure Manifold 2. Extend Air Supply out of Analyzer (Vacuum/Pressure Assembly (RR-7)). ® − 5 - 55 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 330: Hard Drive Formatting

    VP-61 Hard Drive Formatting 8. From Main Menu, press „ DIAGNOSTICS Required Tools & Materials: „ MORE CELL-DYN 3200 System Service Disk PN 9380700 „ IDRAIN ACCUMULATORS Verification Procedure 9. Initialize Analyzer and repeat Step 8. 1. Perform (VP-64) “Backup Procedure”...

  • Page 331: Dos Installation

    Required Tools & Materials: „ „ „ „ RETURN Service Disk PN 9380700 Verification Procedure 1. Turn System OFF. 2. Insert Service Disk into Floppy Drive. ® − 5 - 57 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

  • Page 332: Backup Procedure

    „ ENTER Required Tools & Materials: This copies Old Version Setup from Setup Disk to Hard Drive. CELL-DYN 3200 System Setup Disk PN 9380595 5. Remove Setup Disk. Verification Procedure 6. Turn System OFF, wait 5 seconds, then turn System ON.

  • Page 333: Download Hssl Com

    NOTE: Date format is YYMMDD (Year Month Day). Required Tools & Materials Example: 960321 = 21 March 1996. CELL-DYN 3200 System Installation Disks 1 & 2 P.N. 9380590 d. Place Formatted 1.44mb diskette in Floppy Disk Drive. e. Type Verification Procedure COPY C:\RAWFILE\960321\(SEQUENCE#).RAW B:.
  • Page 334 NOTE: 0 = zero a. Press F8 to exit to the C:\CD3200> prompt. b. Turn off analyzer, wait 5 sec, turn on analyzer to reboot. ® − 5 - 60 CELL-DYN 3200 SYSTEM SERVICE MANUAL Part Number 9140300 August 1998...

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