VisualSonics VEVO Operator's Manual page 34

Chapter 5: Anesthetizing a Mouse: Inducing anesthesia
Connect oxygen tank to
regulator (if oxygen is
supplied from a free-
standing tank)
Connect to the delivery sub-
system
Connect dual delivery
system to nose cone and
chamber connection
Connect breathing elbow
down tube and chamber
outlet port to charcoal
filters
24 VisualSonics Vevo Compact Anesthesia System Operator Manual
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If you hear hissing:
Check the condition of the gasket between the tank and the regulator.
1.
• Check the E-cylinder (651-liter tank)
• Check the H-cylinder (7107-liter tank)
Tighten the regulator to the tank.
2.
Note: These checks apply to North American regulator configurations only.
This common outlet port is a friction-fit connection. Ensure that you
make leak-free connections.
These are also friction-fit connections. Again, ensure that you make leak-
free connections.
Push the tubing on as tightly as possible and ensure filters are placed into
the filter holder (in an upright position). Standing filters on end impedes
the airflow out the bottom on canister, renders the filter useless and
causes back pressure in the breathing circuit.
If required, fill the vaporizer with the appropriate liquid agent. (See
3.
"Filling the vaporizer" on page 19.)
Open the stopcock in the induction chamber tubing line.
4.
Close the stopcock in the mouse mask line.
5.
Turn the oxygen flowmeter to at least one (1) liter per minute (lpm).
6.
Place animal #1 in the induction chamber and secure the lid.
7.
Warning: The chamber is airtight. If the oxygen
flowmeter on the machine is turned off, there will be
NO AIRFLOW into the chamber.
NEVER leave animals in the chamber without an
oxygen flow.
Turn the vaporizer dial to 2-2
8.
consciousness within two minutes.
When Animal #1 shows no response to rocking/jiggling the chamber
9.
(approximately two minutes), push the oxygen flush button for 20-30
seconds (for 2-liter chamber) to evacuate all anesthesia-saturated gas
from the chamber.
Note: The brief oxygen flush evacuations of the chamber should not awaken
already anesthetized animals.
Note: If the stopcock to the mouse mask breathing circuit is open when you press
the oxygen flush button, the resulting oxygen pressure through the mouse mask
may push that animal's nose out of the mask. Always close the stopcock to the
mouse mask before using the oxygen flush.
Open the stopcock in the mouse mask line.
10.
Close the stopcock in the induction chamber tubing line, then
11.
immediately open the chamber and remove Animal #1.
%. Animal #1 should begin to lose
½
V1.0