Chapter 5: Anesthetizing A Mouse Pre-Operative Checks; Inducing Anesthesia - VisualSonics VEVO Operator's Manual

Chapter 5: Anesthetizing a Mouse
Pre-operative checks
Verify the gas supply
Test the oxygen flowmeter
Fill the vaporizer

Inducing anesthesia

V1.0 VisualSonics Vevo Compact Anesthesia System Operator Manual
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Ensure that the oxygen supply line for each gas is connected to the
1.
appropriate DISS inlet located on the back of the flowmeter(s). Connect
the gas supply hose(s) to the in-house supply as necessary.
Ensure that the gas supply is turned on.
2.
Caution: When you turn off the flowmeter, turn the control
knob clockwise just far enough so the ball float reaches the
bottom of the tube. Continuing to tighten the control knob
will damage the valve seat and cause a leak. The unit will
need to be replaced when this damage occurs.
Test the oxygen flowmeter for full range of operation by turning the
3.
control valve counter-clockwise to open it, then clockwise to close it.
Close the valve when you have completed the test.
Test the oxygen flush valve for performance by pressing and holding
4.
the button for three seconds. Oxygen should flush rapidly out of the
common outlet. When the oxygen flush button is released, it should
return immediately to the off position and the flow should stop
immediately.
Caution: Remove the machine from service if:
• the oxygen flush button does not immediately return to
the OFF position after being released, or
• the flow of oxygen does not immediately stop flowing
from the common outlet after the oxygen flush button is
released.
Then call VisualSonics or a VisualSonics-authorized service
facility to repair the valve.
Fill the vaporizer with the appropriate anesthetic agent. (See "Filling
1.
the vaporizer" on page 19.)
Your facility should have in place an inhalation anesthesia protocol /
SOP. The following is an outline from which you can begin to create your
own. If you need assistance writing your protocol, please call us at :
1-866-416-4636.
Check that your oxygen supply tank is open and that you have
1.
sufficient gas pressure in the line from the tank (minimum 1500 psi for E-
size cylinder.
Assemble your system and check all connections for possible leaks of
2.
the oxygen/anesthesia mix.
Chapter 5: Anesthetizing a Mouse: Pre-operative checks
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