Abi 7000/7500 Sds Master Mix Naming Conventions - Qiagen QIAgility User Manual

Appendix E
ABI 7000/7500 SDS master mix naming
conventions
Absolute Quantification
For absolute quantification, the ABI software requires each
reaction to be associated with a detector, reporter and
quencher. To achieve this, master mixes used in the exported
reactions must be named in the QIAgility software according
to the convention:
[?:]D_R_Q{:D_R_Q}
?
:
D
R
Q
[]
{}
For example, "Mix C:EBV_FAM_TAMRA" specifies a mix with
detector for EBV, using FAM reporter, and TAMRA quencher.
The "Mix C" portion of the name is unused by the SDS
software.
The absolute quantification function exports a single file,
"XXX.TXT", where "XXX" is the filename.
Allelic Discrimination
For allelic discrimination, the ABI software requires each
reaction to be associated with a marker, identifying a pair of
QIAgility User Manual 11/2011
Indicates a string of characters, which
excludes ":".
Is a separator.
Is a placeholder for detector.
Is a placeholder for reporter.
Is a placeholder for quencher.
Indicates an optional component.
Indicates 0 or more items.
Appendix E
E-1