Filter Based Flurometer Description - Teledyne 6650B Operating Instructions Manual

Introduction
1.2

Filter Based Flurometer Description

The Fluorometer is a filter-based analyzer. The Fluorometer
measures the ability of the analyte of interest to absorb light in a narrow
spectral region and emit light at a longer wavelength. A filter-based
Fluorometer is a good choice when quantitative measurements are
desired for a specific analyte in process. Additionally, the Fluorometer
provides a relative measurement and can be calibrated with a known
concentration standard(s) or correlated to measurements using standard
laboratory methods resulting in a quantitative fluorescence.
A filter-based Fluorometer uses optical filters to provide specific
excitation or emission wavelengths wavelengths for molecular
fluorescence. In the Fluorometer the filters are located internal to the
transmitter and are specific to the application. Therefore, the
Fluorometer is a dedicated instrument for monitoring only one specific
analyte of interest. The filter sets used to configure the instrument are
specific to the analyte of interest. In order to monitor a new analyte of
interest, the user must return the Fluorometer to TIA for factory
reconfiguration. The Fluorometer can be used for both quantitative
measurements (determination of analyte concentration) and control
measurements (switching of valves once a fluorescence level is attained,
for example)
In brief, the Fluorometer works as follows: The light source
launches excitation light into a fiber optic cable. The fiber optic cable
transfers the excitation light to the Fluorescence probe mounted in the
process. The fluorescence probe launches light into the process sample,
and collects the molecular fluorescence (emission) from the sample. The
emission light is then transferred from the probe through fiber optic
cable to the analyzer. The emission light passes through an emission
filter in order to remove any residual excitation energy collected by the
fluorescence probe. The emission light then impinges a detector and the
fluorescence intensity is displayed on the analyzer.
Unlike many fluorescence units on the market, the Fluorometer
utilizes a xenon flash lamp to provide excitation energy. This lamp
allows the Fluorometer to be easily configured to meet any excitation
wavelength requirement with the appropriate filter selection across the
entire spectrum. The xenon flash lamp also has an extended lifetime
compared to other common UV sources (deuterium, mercury vapor,
etc.), which reduces the cost of ownership of the analyzer.
Teledyne Analytical Instruments
2
Model 6650