Teledyne 6650B Operating Instructions Manual

Uv-photo-x fluorescence analyzer
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UV-Photo-X
OPERATING INSTRUCTIONS FOR
MODEL 6650B
UV-Photo-X Fluorescence Analyzer
P/N M6650
02/15/2006
ECO # xx-xxxx
DANGER
Toxic gases and or flammable liquids may be present in this monitoring system.
Personal protective equipment may be required when servicing this instrument.
Hazardous voltages exist on certain components internally which may persist for a
time even after the power is turned off and disconnected.
Only authorized personnel should conduct maintenance and/or servicing. Before
conducting any maintenance or servicing, consult with authorized
supervisor/manager.
Teledyne Analytical Instruments
i

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Summary of Contents for Teledyne 6650B

  • Page 1 UV-Photo-X OPERATING INSTRUCTIONS FOR MODEL 6650B UV-Photo-X Fluorescence Analyzer P/N M6650 02/15/2006 ECO # xx-xxxx DANGER Toxic gases and or flammable liquids may be present in this monitoring system. Personal protective equipment may be required when servicing this instrument. Hazardous voltages exist on certain components internally which may persist for a time even after the power is turned off and disconnected.
  • Page 2 Any safeguards required such as locks, labels, or redundancy, must be provided by the user or specifically requested of Teledyne at the time the order is placed.
  • Page 3: Safety Messages

    NOTE: Additional information and comments regarding a specific component or procedure are highlighted in the form of a note. CAUTION: THE ANALYZER SHOULD ONLY BE USED FOR THE PURPOSE AND IN THE MANNER DESCRIBED IN THIS MANUAL. Teledyne Analytical Instruments...
  • Page 4 Manuals do get lost. Additional manuals can be obtained from Teledyne at the address given in the Appendix. Some of our manuals are available in electronic form via the internet. Please visit our website at: www.teledyne-ai.com.
  • Page 5: Table Of Contents

    2.9 Signal-to-Background Ratio 2.10 Dynamic Range 2.11 Linearity 2.12 Process Background 2.13 Mode 1 Initialization 2.14 Mode 2 Initialization Diagnostics & Controls..............8 3.1 Front Panel Controls Hardware Installation ..............9 4.1 Physical 4.2 Optical 4.3 Electrical Teledyne Analytical Instruments...
  • Page 6 Series 1 - Initializing the Unit and Determination of the Probe/Analyzer Signal Level A-1.2 Determination of the Probe/Transmitter Fluorescence in a Non-Fluorescing Liquid: A-1.3 Determination of the Process Fluorescence: Notes Recommended 2-Year Spare Parts List Index..................... 38 Teledyne Analytical Instruments...
  • Page 7: List Of Figures

    UV-Photo-X List of Figures Figure 1-1: Molecular Fluorescence Example......1 Figure 3-1: Photo-X Front Panel Controls ........8 Figure 4-1: Top view of Flurometer..........11 Teledyne Analytical Instruments...
  • Page 8 Since the use of this instrument is beyond the control of Teledyne, no responsibility by Teledyne, its affiliates, and agents for damage or injury from misuse or neglect of this equipment is implied or assumed.
  • Page 9: Introduction

    (spectral) wavelength distribution, which is characteristic of the electronic properties of the molecule, and 2) The intensity of the fluorescence, which is typically correlated to the concentration of the fluorescent molecule in the solution. Figure 1-1: Molecular Fluorescence Example Teledyne Analytical Instruments...
  • Page 10: Filter Based Flurometer Description

    The xenon flash lamp also has an extended lifetime compared to other common UV sources (deuterium, mercury vapor, etc.), which reduces the cost of ownership of the analyzer. Teledyne Analytical Instruments...
  • Page 11 Fluorometer. In addition to standard operation methods and procedures, a section detailing some application development objectives (Appendix 1) has been provided to aid the user in defining the parameters required to realize optimal process monitoring. Teledyne Analytical Instruments...
  • Page 12: Definition Of Terms

    The span filter is used to verify instrument operation and should not be used to calibrate the instrument. Teledyne Analytical Instruments...
  • Page 13: Background Fluorescence

    Noise is measured by placing the fluorescence probe in air and in complete darkness (no stray light). For process monitoring, the signal-to-noise ratio is not as important as the signal-to background ratio. Teledyne Analytical Instruments...
  • Page 14: Signal-To-Background Ratio

    2.13 Mode 1 Initialization Mode 1 initialization is a method for setting up the Fluorometer for process monitoring. Mode 1 initialization is used to setup the Fluorometer when the fluorescence intensity versus analyte Teledyne Analytical Instruments...
  • Page 15: Mode 2 Initialization

    2 initialization, the zero fluorescence level is set electronically to 0 counts (display value) and the upper limit of the range is set to the 1999 display value. Section 5 provides details the procedure for Mode 2 initialization. Teledyne Analytical Instruments...
  • Page 16: Diagnostics & Controls

    Fine zero adjustment 2) Span check LED indicator Reference detector switch 3) User mode indicator LED Measure detector status 4) User mode span adjustment 5) Digital display 6) 20mA adjustment 7) 4mA adjustment 8) Course zero adjustment Teledyne Analytical Instruments...
  • Page 17: Hardware Installation

    1) Unscrew the optic cables from the bottom of the light source and the control unit. 2) Unscrew the liquid tight connector’s 1 ¾” nut which is connected to the conduit from the other part. 3) Remove the probe bracket from wherever it’s attached to. Teledyne Analytical Instruments...
  • Page 18: Electrical

    4) It is best if the unit is not initially powered up until after the initial optical setup is completed. Teledyne Analytical Instruments...
  • Page 19: Figure 4-1: Top View Of Flurometer

    UV-Photo-X Hardware Installation Figure 4-1: Top view of Flurometer Teledyne Analytical Instruments...
  • Page 20: Setup & Operation

    Additionally, the user can adjust the output display over the full scale range once it has been established. Note: The above readings are all based on defined excitation and emission wavelengths for the given sample or intended application. Teledyne Analytical Instruments...
  • Page 21: First Power-Up Following Installation

    Both the REF and MSR DET LEDs exhibit solid green or flashing green output. c) The value on the display is fluorescence level in the process. If the sample is the background material, this is your background fluorescence level. Teledyne Analytical Instruments...
  • Page 22: Application Engineering

    You will have to develop a compound specific calibration curve and you will have to determine your limit of detect ability, and dynamic range. Teledyne Analytical Instruments...
  • Page 23: Initialization For Process Monitoring: Mode 2 Operation

    2) Perform Mode 2 initialization a) Press the INT PRESET, located in the upper left hand corned of the Fluorometer (refer to Figure 3-1), for at least eight (8) seconds. Please see Section 2 for a description of Mode 2 operation. Teledyne Analytical Instruments...
  • Page 24: Case 3: Process Monitoring Following Application Engineering

    Case 3: Process monitoring following application engineering This case is valid when the user has determined that Mode 2 monitoring is sufficient to meet the monitoring goals in process. This is the simplest monitoring method since no calibration standard is required. Teledyne Analytical Instruments...
  • Page 25 The FLTR-IN indicator will change from red to green. Wait 2 minutes for the output to stabilize and the unit should display the background fluorescence level. vii. Both the REF and MSR DET LEDs should have solid green or flashing green output. Teledyne Analytical Instruments...
  • Page 26: Initialization For Process Monitoring: Mode 1 Operation

    Both the REF and MSR DET LEDs should have solid green or flashing green output. iii. If the MSR indicator LED color does change color, refer to the Diagnostics section below. Teledyne Analytical Instruments...
  • Page 27: Adjusting The Display

    4) The display adjustment is the right of the display. The adjustment is designated USR SPAN CONTROL 5) Display Adjustment (Mode 1 initialization) a) The calibration sample is being monitored b) If the display reading is close to 1000 counts (off by <100 counts) use the Teledyne Analytical Instruments...
  • Page 28: Adjusting The 20 Ma Level

    CHK is no longer depressed. The display adjustments are located to the left of the display. If the display reading is close to 0000 counts (<100 counts off) use the FINE ZERO CONTROL to adjust the display. Teledyne Analytical Instruments...
  • Page 29: Adjusting The 20 Ma Level

    1) Immerse the probe in a process sample, preferably with a known analyte concentration and therefore fluorescence level. 2) Monitor the 4-20 mA signal using an appropriate meter. 3) The Fluorometer is configured in USER MODE. The display is displaying the fluorescence intensity in arbitrary counts. Teledyne Analytical Instruments...
  • Page 30: Diagnostics

    4) If either lamp shows flashing orange, the energy level of that channel is below the reliable measurement range. The optical system must be revised, to bring the lamp indication back to steady green, if possible. Teledyne Analytical Instruments...
  • Page 31: Rectifying The Problem: Flashing Yellow Indicators

    Gently rub the optical surface with a damp lint free cloth or cotton swab to remove deposits. e) Rinse the probe tip using spectroscopic grade isopropyl alcohol (IPA or 2-propanol) or methanol to remove residual cleaning solvent. Teledyne Analytical Instruments...
  • Page 32: Rectifying The Problem: Red Indicators

    If there has been a major change in the probe installation or process piping. Most likely, there is a stray light leak into the system. Verify that the monitoring location is in complete darkness. If stray light is not present at the monitoring location, contact TIA for assistance. Teledyne Analytical Instruments...
  • Page 33 UV-Photo-X Setup & Operation Teledyne Analytical Instruments...
  • Page 34: Specifications

    5ppm + span filter = OFF-SCALE Mode 2 Calibration Salcon Water = 044 counts 5ppm in water = 081counts Span filter = 279 counts 5) Optical span filter. Span Calibration value: see above Span Filter Type: SS-KFLY-5 Teledyne Analytical Instruments...
  • Page 35: Transmitter Specifications

    Output signal: Isolated 4-20mA sourced output Permissible loop resistance 500 ohms for customer use c) Span calibration: Remote trigger via normally closed contact (must remove the jumper on the top of the Photo-X unit for remote triggering) Teledyne Analytical Instruments...
  • Page 36: Mechanical Specifications Of Analysis Cell/Probe

    4) Probe sealing material: Viton o-ring seal for sapphire windows on probe 5) Probe pressure rating: 5000 psig 6) Probe temperature rating: 200°C 7) Assembled probe dimensions: 1/2” diameter shaft X 12.5” (to tip of SMA fitting) Teledyne Analytical Instruments...
  • Page 37 5 ppm (v/v) EPA #2 in Salcon produced water. To maintain calibration level with homogenization of the calibration solution a setting of 40 (dial) with an output of 30 was required with circulation level maintained for ~15 minutes. Teledyne Analytical Instruments...
  • Page 38: Appendix

    5) After lamp warm-up and stabilization, the REF DET LED should have a steady green output. The MSR DET LED should have either a solid green or flashing green output. Teledyne Analytical Instruments...
  • Page 39 Both the REF and MSR DET LEDs should have solid green or flashing green output. k) The unit is now ready for either application engineering studies or process monitoring. Teledyne Analytical Instruments...
  • Page 40: A-1.2 Determination Of The Probe/Transmitter Fluorescence In A Non-Fluorescing Liquid

    5) Record the fluorescence level on the display 6) Insert the span filter. a) Wait 2 minutes for the output to stabilize b) Record the fluorescence level due to span filter insertion. c) Remove the span filter. Teledyne Analytical Instruments...
  • Page 41: A-1.3 Determination Of The Process Fluorescence

    Range Multiplier = 1999/Display Value 4) Gently wipe the probe tip with a lint free cotton swab or cloth dampened with the process background material and gently rub Teledyne Analytical Instruments...
  • Page 42 The initialization sequence for Mode 1 is located in Section 5. 8) Once the process fluorescence has been characterized and the calibration procedure determined repeat the experimental series using the desired initialization mode to verify that the correct monitoring approach has been selected. Teledyne Analytical Instruments...
  • Page 43 Series 1: Air and Liquid Probe/Analyzer Fluorescence Level Material Probe/Transmitter Span Filter Fluorescence (Air, Liquid, Solid) Fluorescence Series 2: Process Sample Analysis Analyte Sample Number Measured Fluorescence Concentration Series 3: Monitoring verification using selected calibration (initialization) procedures. Analyte Sample Number Measured Fluorescence Concentration Teledyne Analytical Instruments...
  • Page 44: Notes

    A solid red detector indicates to much signal intensity. Unit must be returned to factory for evaluation. A flashing yellow LED on either detector is a low signal level and unit must be returned to factory for evaluation. Teledyne Analytical Instruments...
  • Page 45: Recommended 2-Year Spare Parts List

    (if available) and the model and serial number of the instrument for which the parts are intended. Orders should be sent to: TELEDYNE Analytical Instruments 16830 Chestnut Street City of Industry, CA 91749-1580 Phone (626) 934-1500, Fax (626) 961-2538 Web: www.teledyne-ai.com...
  • Page 46: Index

    36, See company address safety information, iii caution sign, iii spare parts listing, 36 combustible gas warning, viii specifications, 29 company address. See company Teledyne address, 36 address troubleshooting, 20 copyright, ii warning sign, iii electronic component location, 6 warranty, ii...

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