When To Use The Active Seal Wash Option - Agilent Technologies InfinityLab LC Series User Manual

4 Optimizing Performance

When to Use the Active Seal Wash Option

When to Use the Active Seal Wash Option

Concentrated buffer solutions will reduce the lifetime of the seals and pistons in
your binary pump. The active seal wash option allows to maintain the seal
lifetime by flushing the low pressure side of the seals with a wash solvent.
The seal wash option is strongly recommended if buffer concentrations of 0.1 M
or higher are used regularly with the pump.
The active seal wash option kit can be ordered by quoting Active Seal Wash
Upgrade Product including Service (G1399A).
The seal wash option comprises a peristaltic pump, secondary seals, gaskets,
seal holders and tubing for both pump heads. A bottle of premixed
water/isopropanol (90/10 vol%) is placed in the solvent cabinet and connected to
the peristaltic pump.
Always use a mixture of HPLC-grade water (90 %) and isopropanol (10 %) as
wash solvent. This mixture prevents bacteria growth in the wash bottle and
reduces the surface tension of the water.
In order to avoid accumulation of buffer salts or impurities, regularly replace the
NOTE
washing solution using fresh solvents.
The operation of the peristaltic pump can be controlled from the data system or
the Instant Pilot.
For adding a seal-wash option, please contact your local Agilent Technologies
service representative.
InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 61