Waters 2465 Manuals

Manuals and User Guides for Waters 2465. We have 2 Waters 2465 manuals available for free PDF download: Operator's Manual, Quick Install Manual

Waters 2465 Operator's Manual (226 pages)

Electrochemical Detector

Brand: Waters | Category: Security Sensors | Size: 8 MB

Intended Use
15
Quality Control
15
Table of Contents
17
Preface
33
Documentation Conventions
34
2465 Detector Theory of Operation
37
Detector Introduction
37
Electrolysis Reactions
37
Electrolysis at Working Electrode
38
Current-Potential Curves
40
Current-Potential Curve
40
Detector Features
41
Hydrodynamic Voltammogram of Norepinephrine
41
Detector Design
43
Electronics and Data Acquisition
43
Signal Processing from Flow Cell to Output
43
Electronics
44
Control Board
44
Rear Panel
45
Filtering
45
Time Constant (Filter Setting) Comparison
46
Autozero and Maximum Compensation
47
Ranges and Maximum Compensation
47
Startup Diagnostics
48
Temperature Control
48
Flow Cell Design
48
Flow Cell Operation
48
Flow Cell with a Salt-Bridge Reference Electrode
49
Three-Electrode Potentiostat
50
Three-Electrode Electrochemical Cell
50
Electrodes
51
Materials for Working Electrodes
51
Working Electrode Diameter
52
Potential Limits and Applications for Working Electrodes
52
Catecholamines
52
Spacer Thickness
53
Recommended Flow Cells with Different Columns
53
Flow Cell Volume with Spacers
53
ISAAC Reference Electrode
54
Signal and Noise for Norepinephrine with Different Spacers
54
Potential of the Ag/Agcl Reference Electrode
55
Hy-REF Reference Electrode
57
Mass of Anhydrous Sodium and Potassium Chloride Per Liter for Various Molar Concentrations
57
Salt-Bridge Ag/Agcl Reference Electrode
58
Cell Potential (Hy-REF and Ag/Agcl Electrodes) Versus Ph
58
Principles of Detector Operation
59
DC Mode
59
Pulse (PAD) Mode
60
Potential Steps in Pulsed Amperometric Detection
62
Selection of Pulse Parameters
63
Stabilization Time
63
Change in Cell Current During PAD
64
Magnified View of a Chromatogram Obtained with PAD
65
Chromatograms Acquired at Different Data Rates
66
Scan Mode
68
Examples of Scanning Voltammograms
68
References
69
Installing the 2465 Detector
71
Site Selection and Power Requirements
71
Site Selection
71
Major Steps for Installing the 2465 Detector
71
Dimensions of the 2465 Detector
72
Installation Site Requirements
72
Power Requirements
73
Unpacking and Inspecting the 2465 Detector
74
Making Electrical Power Connections
75
Unpacking the 2465 Detector
75
Making Fluidic Connections
76
Connecting the Power Cord
76
Venting the Detector
76
Required Materials
77
Making the HPLC Connections
77
Optional Materials
78
Installing the 2465 Detector
79
Installing the External Pump and Pulse Dampener
80
Connecting a Column
82
Installing the Flow Cell
83
Connecting Tubing to the Flow Cell
84
Installing the Flow Cell
85
Making I/O Signal Connections
87
Rear Panel Connections
87
Rear Panel Connections on 2465 Detector
88
I/O Signal Inputs and Outputs
89
Connecting to a 2695 Separations Module (Stand-Alone)
91
Connector B
92
Connecting to a Bussat/In Module
93
Bussat/In Module (Front Panel)
93
Connecting a Bussat/In Module Channel 1 to the 2465 Detector
94
Connecting to a 746 Data Module
95
Config Screen
95
Making RS-232 Connections
96
Verifying COM Port Settings
97
IEEE-488 and RS-232 Connections in a Waters Empower System
97
Operating the 2465 Detector
99
Starting up the Detector
99
Powering on the Detector
99
Using the Display
100
Calculating Checksum Screen
100
Checksum Screen
100
Main Screen
100
Using the Keypad
101
Finding the Parameters
102
Using the Function Keys
102
Using the Keypad to Change Parameters
103
Function Key Commands
103
Status and Control Parameters
106
Overview of the 2465 Detector Modes
110
DC Mode
111
DC Mode Navigation
111
Pulse (PAD) Mode
113
Pulse Mode Navigation
113
Scan Mode
115
Remote Mode
115
Scan Mode Navigation
115
Remote DC Mode
116
Remote Pulse Mode
116
Remote Scan Mode
116
Introduction to Time Files
117
Default Time File in DC Mode
118
Run (Waiting) Screen before Starting a Run
119
Run Screen after Starting a Run
119
Programming Output Event Functions in Time Files
120
End Cycle Time Screen for an Empty Time File
120
Preparing the 2465 Detector for Operation
121
Outputs and Commands
121
Preparing the Detector for Remote Control from Empower
123
Acquisition Server Dialog Box
124
Changing from Remote Mode to Stand-Alone Mode
125
Equilibrating the Detector
125
DC Setup Screen
125
Derating Curve for Detector Oven Temperature
126
DC Stat Screen
126
Detector Oven Temperature Settings
126
Using DC Mode
127
DC Mode Quick Reference List
127
Setting Initial Conditions in DC Mode
128
DC Setup Screen
128
Change Polarity to Negative Screen
129
Change Polarity to Positive Screen
129
DC Stat Screen
129
Turning the Flow Cell on and off in DC Mode
130
DC Stat Screen with Cell off
131
Switch Cell on Screen
131
Switch Cell off Screen
131
Creating a Time File in DC Mode
132
DC Setup Screen
132
Programming a Sample Time File in DC Mode
132
Prog Screen with Initial Conditions
133
Events Setup Screen
133
Delete Timefile Screen
134
Overwrite Time Screen
134
Prog Screen after Changing Cell Potential
135
End Cycle Time Screen
136
Running a Time File in DC Mode
137
Run (Waiting) Screen
138
Run Screen after Starting a Run
138
Using Pulse (PAD) Mode
139
Pulse Mode Quick Reference List
139
Setting Initial Conditions in Pulse Mode
140
Pulse Setup1 Screen
140
Pulse Setup2 Screen
140
Change Polarity to Negative Screen
141
Pulse Stat Screen When Flow Cell Is on
141
Changing the Range in Pulse Mode
142
Pulse Stat Screen When Flow Cell Is off
142
Making a Chart Mark in Pulse Mode
143
Autozeroing the Detector in Pulse Mode
143
Turning the Flow Cell on and off in Pulse Mode
143
Pulse Stat Screen with Cell off
144
Switch Cell on Screen
144
Creating a Time File in Pulse Mode
145
Switch Cell off Screen
145
Programming a Sample Time File in Pulse Mode
145
Pulse Setup1 Screen
146
Pulse Setup2 Screen
146
Prog Screen with Initial Conditions
147
Delete Timefile Screen
147
Overwrite Time Screen
148
Events Setup Screen
150
End Cycle Time Screen
150
Running a Time File in Pulse Mode
151
Run (Waiting) Screen
151
Run Screen after Starting a Run
151
Using Scan Mode
152
Setting Initial Conditions in Scan Mode
152
Scan Setup Screen
152
Scan Mode Quick Reference List
152
Turning the Flow Cell on and off in Scan Mode
153
Scan Stat Screen
153
Switch Cell on Screen
154
Switch Cell off Screen
154
Scan Setup Screen
154
Performing a Scan in Scan Mode
155
Scan Setup Screen
155
Scan Stat Screen
155
Optimizing the Working Potential
156
Constructing a Hydrodynamic Voltammogram
156
Starting a Scan
156
Constructing a Hydrodynamic Voltammogram for Norepinephrine
157
Constructing a Scanning Voltammogram
158
Scanning Voltammetry of Norepinephrine
158
Programming Scan Mode
158
Overlay of Four "Half" Forward Scans
159
Chromatogram of a Sample in DC Mode
160
Programming Scan Mode
160
Shutting down the 2465 Detector
161
Turning off the Flow Cell
161
Shutting down for a Short Time
162
Shutting down for a Long Time
162
Maintaining the 2465 Detector
163
Introduction
163
General Safety Precautions
163
Frequency of Electrode Maintenance
165
Spare Parts
165
Waters Technical Service
165
Schedule of Electrode Maintenance Tasks
165
Disassembling the Flow Cell
166
Disconnecting the Flow Cell
167
Removing the Bolts (Top View)
167
Cleaning the Working Electrode
168
Maintaining the ISAAC Reference Electrode
170
Cleaning the ISAAC Reference Electrode
170
Locating the ISAAC Reference Electrode
170
Polishing the ISAAC Reference Electrode
171
Storing the ISAAC Reference Electrode
172
Maintaining the Salt-Bridge Reference Electrode
172
Coating the ISAAC Reference Electrode
172
Inspecting the Salt-Bridge Reference Electrode
173
Inspecting a Reference Electrode
173
Cleaning the Salt-Bridge Reference Electrode
174
Removing the Salt-Bridge Reference Electrode
174
Replacing the Cotton Wool Frit
175
Components of the Salt-Bridge Reference Electrode
175
Installing the Salt-Bridge Reference Electrode
176
Removing the Cotton Wool Frit
176
Storing the Salt-Bridge Reference Electrode
178
Reassembling the Flow Cell
178
Assembling the Flow Cell
180
Replacing the Micro Flow Cell
181
Mounting the Fused Silica Connector
182
Other Procedures
183
Replacing Fuses
183
Filling the Micro Flow Cell
183
I/O Connector Warning
184
Removing the Fuse Holder
184
Checking the Fuse Rating
184
Changing a Spacer in the Flow Cell
185
Changing the Flow Cell Volume
185
Changing a Column
186
Cleaning the Detector
186
Diagnostics and Troubleshooting
187
Error Messages
188
Diagnostics
188
Dummy Cell Test
188
Diagnostics Screen
188
Dummy Cell
189
Diagnostics Screen
190
Noise Test with Dummy Cell
190
Dummy Cell Test Settings
190
Stop Flow Test
191
Noise Test Screen
191
Keyboard Test
192
Display Test
192
Testing a Key on the Keypad
192
Troubleshooting Tables
193
Display Test
193
Configuration Screen
193
No Detector Response
194
High Cell Current
194
Noisy Baseline
195
Drifting Baseline
195
Decreased Sensitivity (Low S/N Ratio)
196
Baseline Oscillations
196
Saturation of Output
196
Physical Symptoms
197
Appendix A 2465 Detector Specifications
199
General Specifications
199
Physical Specifications
200
Operating and Environmental Requirements
200
Pulse Mode
201
Scan Mode
201
Flow Cell Specifications
201
2465 Detector Components and Spare Parts 167
203
Flow Cells
203
Flow Cell Kits
203
Flow Cell, 2-MM GC WE, Salt-Bridge Reference Electrode
204
Flow Cell, 2-MM GC WE, ISAAC Reference Electrode
205
Flow Cell, 3-MM Pt WE, ISAAC Reference Electrode
205
Flow Cell, 3-MM au WE, Hy-REF Electrode
206
Flow Cell, 2-MM Ag WE, Hy-REF Electrode
206
Micro Flow Cell, 0.7-MM GC WE, Salt-Bridge REF Electrode
207
Startup Kit Components
208
Startup Kit
208
Spare Parts
209
Appendix C Sample ECD Methods
211
Appendix D 2465 Detector Glossary
215
DC Mode
215
Timed Events Mode
217

Waters 2465 Quick Install Manual (2 pages)

Electrochemical Detector

Brand: Waters | Category: Security Sensors | Size: 1 MB

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