Table of Contents
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Table of Contents
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Summary of Contents for Motic AE20
- Page 2 We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods. This involves modification to the mechanical structure and optical design of our instruments. Therefore, all descriptions and illustrations in this instruction manual, including all specifications are subject to change without notice.
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Page 3: Table Of Contents
TABLE OF CONTENTS Section Page Nomenclature ………………………………………………………………………… II. Setting-up the instrument …………………………………………………………… III. Assembling the Microscope …………………………………………………………… 1. Installing the Lamp ………………………………………………………………… 2. Halogen Lamp ……………………………………………………………………… 3. Filter Holder ………………………………………………………………………… 4. Mounting the Condenser …………………………………………………………… 5. Installing the Objectives …………………………………………………………… 6. -
Page 4: Nomenclature
I. Nomenclature Model AE20... - Page 5 Model AE21...
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Page 6: Setting-Up The Instrument
II. Setting-up the Instrument Working Environment The location should be free from dust, moisture, chemical vapours and from mechanical vibrations. Do not situate the instrument in a warm and/or humid environment. Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a well-lit bright wall. -
Page 7: Halogen Lamp
Replace the metal stage insert. 6. Mechanical Stage Secure the mechanical stage to the AE20/21 plain stage using the two mounting screws located beneath the stage on the right side (when facing the front of the instrument). -
Page 8: Mounting The Eyepieces
7. Mounting the Eyepieces Remove the dust caps from the eyepiece tubes. Insert the eyepieces into the eyepiece tubes and tighten the eyepiece clamp screws. Be sure to use eyepieces with the same magnification. If the rubber eye guards are to be used, fit them in the groove around the eyepiece. IV. -
Page 9: Brightfield Microscopy
3. Brightfield Microscopy Set the Phase annular diaphragm slider in centre position (O). Bring the specimen image into focus. Adjust the opening of the field of view diaphragm, for normal observation the size of the diaphragm should be just outside the edge of the field of view. The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the illuminating system of the microscope. -
Page 10: Photomicrographic Procedure
If the slider annular ring image is diverged from the phase plate image in the objective, a low phase contrast image will result. For phase contrast microscopy at the maximum contrast, use GIF (Green interference filter) in the optical path. V. -
Page 11: Troubleshooting Table
Never attempt either of the following actions, since doing so will damage the focusing mechanism: Rotate the left and right knob while holding the other. Turning the coarse and fine focus knobs further than their limit. VI. Troubleshooting Table As you use your microscope, you may occasionally experience a problem. The troubleshooting table below contains the most frequently encountered problems and their possible causes. - Page 12 Brightfield objective being used Phase annular diaphragm not in optical path Phase annular diaphragm and objective phase Image quality: symbol do not match No image under phase contrast or details Slider annular ring image has moved away from the cannot be viewed objective phase plate image Thickness of specimen holder is outside the compensating range of objective...
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Page 13: Care And Maintenance
Lamp blows out immediately Specified lamp not being used Connectors are not securely connected Lamp flickers Lamp near end of service life Lamp not securely plugged into socket VII. Care and Maintenance 1. Lenses and Filters To clean lens surfaces or filters, first remove dust using an air blower. If dust still persists, use a soft/clean brush or gauze.
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