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Summary of Contents for Motic AE30
- Page 2 We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods. This involves modification to the mechanical structure and optical design of our instruments. Therefore, all descriptions and illustrations in this instruction manual, including all specifications are subject to change without notice.
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Page 3: Table Of Contents
TABLE OF CONTENTS SECTION Nomenclature … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … Specification … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … Setting-up the Instrument …... -
Page 4: I Nomenclature
Nomenclature 7. Stage Plate Insert 1. - Page 5 1. Vertical Photo Port 10. Phase Slider 2. Interpupillary Distance Scale 11. Condenser Clamp Holder Screw 3. Diopter Adjustment Ring 12. Condenser Centering Screws 4. Eyepiece 13. Stage Plate 5. Optical Path Selector Lever 14.
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Page 6: Specification
Specifications • Magnification Ratio : 40X-600X • Eyepiece : Objective field φ22 • Objectives : Maganification N.A. W.D. 23.5 0.25 60X (Optional) • Condenser : 1. N.A. 0.3 / W.D. 72mm 2. N.A. 0.5 / W.D. 28mm (Optional) • LED : 6V / 30W Halogen Illumination Adjustable •... -
Page 7: Setting-Up The Instrument
III Setting-up the Instrument Working environment • The location should be free from dust, moisture, chemical vapours and mechanical vibrations. • Do not situate the instrument in a warm and/or humid environment. • Locate the instrument where the operator’ s line of vision is not directed towards a window, a lamp or a well-lit. -
Page 8: Installing The Lamp
Installing the lamp • In order to prevent electric shock always turn the power switch off and unplug the power cord before replacing the lamp. Power Switch • Release lamp housing cover clamp screw using a coin to remove the cover. Lamp Housing Cover Clamp Screw •... -
Page 9: Installing The Objectives
• Replace the stage plate insert. Mechanical Stage • Secure the mechanical stage to the AE30/31 plain stage using the two mounting screws located beneath the stage on the right side Mounting the eyepieces • Remove the dust caps from the eyepiece tubes. -
Page 10: Microscopic Procedure Interpupillary Disatance Adjustment
Microscopic procedure Interpupillary distance adjustment • Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective. • Adjust the interpupillary distance so that both the right and left field of view become one. • This adjustment will enable the user to observe the specimen with both eyes Diopter adjustment •... -
Page 11: Centering The Condenser
Centering the condenser • Set the Phase annular diaphragm slider in centre position (O). • Fully open the field of view diaphragm. • Move the aperture diaphragm lever in open “ O” position. • Bring the specimen image into focus, using the 10x objective. •... -
Page 12: Brightfield Microscopy
Lamp Socket Clamp Screw Knob • Move the lamp socket with the knurled knob to bring the lamp filament image to the centre of the phase plate image of the objective. • After finishing the above lamp centering procedure, insert the filter slider with mat surface of the diffuser turned towards the user. -
Page 13: Phase-Contrast Microscopy
Phase-contrast microscopy • Phase contrast objectives are labelled “ Ph” : Ph1; Ph2; Ph3 and Ph4. • For phase contrast microscopy, be sure to use the annular diaphragm that has the same symbol as the objective, despite of the magnification of the objective. •... -
Page 14: Photomicrographic Procedure
VI Photomicrographic procedure • The optical path selector lever can be used to set the optical path to either the Binocular tube 100:0 or Binocular tube/vertical tube 20:80 (observation: photo). • Before starting photomicrography, check the following: The condenser is centered. The condenser annular diaphragm is centred. -
Page 15: Troubleshooting Table
VII Troubleshooting Table As you use your microscope, you may occasionally experience problems. The troubleshooting table below contains the most frequently encountered problems and their possible causes. Optical and Operating Problems Problem Possible Cause Vignetting or uneven brightness Lamp not installed properly in the field of view or field of view Filter slider in intermediate position only partially visible... -
Page 16: Care And Maintenance
Indicates that the main switch is ON. Indicates that the main switch is OFF. Proper handling of the microscope will ensure years of trouble free service. If repair become necessary, please contact your Motic agency or our Technical Service directly.
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