Nikon Eclipse 50i POL Instruction Manual page 68

Polarizing microscope
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Problem
The focus is uneven.
The image is elongated.
The image is tinged
yellow.
The image is too bright.
The brightness is
insufficient.
Chapter 6 Troubleshooting
6.1 Optical System
Cause
The nosepiece is not attached
correctly. Or its rotation has not
stopped at the click stop position.
The specimen is not secured in place
on the stage surface.
The stage has been attached in out of
perpendicular.
The specimen is tilted relative to the
stage surface.
The nosepiece is not attached
correctly. Or its rotation has not
stopped at the click stop position.
The condenser has not been
centered.
The stage has been attached in out of
perpendicular.
The lamp voltage is too low.
No NCB filter is placed in the optical
path.
No ND filter is placed in the optical
path.
The lamp voltage is too high.
The condenser aperture diaphragm is
stopped down too far.
The field diaphragm image is not
focused on the specimen surface.
The optical path selection knob is not
set to the 100% eyepiece.
Attach the nosepiece correctly. And rotate
it to the click stop position.
Place the specimen properly on the stage.
Install the stage correctly.
Locate the specimen correctly on the
stage.
Attach the nosepiece correctly. And rotate
it to the click stop position.
Center the condenser.
Install the stage correctly.
Increase the lamp voltage with the
brightness control knob. Or, push the
preset switch.
Place it into the optical path.
Place it into the optical path.
Decrease the lamp voltage with the
brightness control knob.
It should normally set to 70 to 80% of the
numerical aperture of the objective.
Focus and center the condenser.
Set to the 100% eyepiece position.
66
Countermeasure

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