Nikon Eclipse 50i POL Instruction Manual page 21

15
Switch to any desired objective
and view the specimen.
• Each time you change objectives, the field
diaphragm and the condenser aperture
diaphragm must be adjusted.
For the bright-field microscopy, the field aperture
should be adjusted so that its image
circumscribes the view field. And the condenser
aperture diaphragm should be 70% to 80% of
the numerical aperture of the objective.
⇒ P.39
⇒ P.41
• Focus on the specimen again using the fine focus
knob or the coarse focus knob.
⇒ P.34
• To use an oil immersion type objective or a water
immersion type objective, perform the oil
immersion or water immersion.
16
Turn off the power after
completing the observation.
Chapter 2 Microscopy
2.1 Diascopic Bright-field Microscopy
19
Switch to any
desired objective
Condenser aperture
Field diaphragm
diaphragm knob
control
Focus on the specimen using
the coarse focus knob and the
fine focus knob.
Push the power switch
to the "0" position.