Adjusting The Condenser Position - Nikon Eclipse 50i POL Instruction Manual

Adjusting the Condenser Position

3.8
Adjust the condenser position (focusing and centering) so that the light passing through the
condenser forms an image at the correct location (center of the optical path) on the specimen
surface.
(1) Perform steps in "2.1 Diascopic Bright-Field
Microscopy" and focus on the specimen using
the 10x objective.
(2) Stop down the field diaphragm to the
minimum setting.
(3) Turn the condenser focus knob to form the
field diaphragm image on the specimen
surface.
(4) Turn the condenser centering screws so that
the field diaphragm image is positioned in the
center of the view field.
(5) Move the 40X objective into the optical path.
Turn the coarse/fine focus knobs and focus on
the specimen.
(6) Turn the condenser focus knob to form the
field diaphragm image on the specimen
surface.
(7) Turn the condenser centering screws so that
the field diaphragm image is positioned in the
center of the view field. This is easiest if you
set the field diaphragm aperture to slightly
smaller than the eyepiece view field.
Chapter 3 Individual Operations
3.8 Adjusting the Condenser Position
Field diaphragm image
Eyepiece viewfield
38
Move the field diaphragm control to
stop down the field diaphragm to
its minimum setting.
Condenser
Condenser
focus knob
centering screw
Field diaphragm image
Eyepiece viewfield