Ryf Axio Lab.A1 Operating Manual page 79

Axio Lab.A1
(3) Setting the microscope
Set the microscope as in the transmitted light
brightfield according to KÖHLER (see Section
4.1.1 (3)).
Center rotary stage Pol (Fig. 4-5/1) (see Section
3.1.8.5) and objectives (see Section 3.1.8.6).
Swivel polarizer (Fig. 4-5/3) into the light path
and, if it is rotatable, position it at 0°.
Insert the analyzer slider (Fig. 4-5/2) into the slit
for compensators (if tube does not already have
an analyzer). The field of view will appear dark
due to the crossed polarizers. With screw-on
analyzers, care must be taken to ensure that
they are aligned with polarizer D (i.e. crossed
position).
Move the specimen into the field of view and
turn it with the rotary stage. As a rule, while
being rotated between crossed polarizers,
birefringent (anisotropic) specimens will now
display the variations in color and intensity
described above. However, optically anisotropic
substances may also remain dark, if an isotropic
direction, e.g. of optically uniaxial or biaxial
crystals, is oriented parallel to the direction of
observation.
04/2013
OPERATION
Lighting and contrasting method in transmitted light
430037-7144-001
Fig. 4-5 Components for transmitted light
polarization
Carl Zeiss
79