Ryf Axio Lab.A1 Operating Manual page 73

Axio Lab.A1
Close luminous-field diaphragm (Fig. 4-1/5)
until it is visible (even if not in focus) in the field
of view (Fig. 4-1/A).
Turn the vertical control of the condenser drive
to lower the condenser until the edge of the
luminous-field diaphragm appears in focus
(Fig. 4-1/B).
Center the luminous-field diaphragm using the
two centering screws (Fig. 4-1/4) on the
condenser carrier (Fig. 4-1/C) and then open the
luminous-field diaphragm until the edge of the
diaphragm just disappears from the field of
vision (Fig. 4-1/D).
For aperture diaphragm adjustment (contrast)
remove an eyepiece from the tube socket and
look into the socket with the naked eye. Set the
aperture diaphragm with the adjusting lever
(Fig. 4-2/4) to approx.
diameter of the exit pupil of the objective
(Fig. 4-1/E). In most applications, this aperture
diaphragm setting provides optimum contrast at
almost ideal resolution, and is therefore the best
compromise for the human eye.
Reinsert the eyepiece into the tube socket.
Every change of objective will result in a change in specimen field size and objective aperture,
together with a possible slight change in centering, so that for optimum results the luminous-
field and aperture diaphragm adjustments must be repeated.
With objectives < 10x the front lens of the condenser (if swivelable) must be folded out and the
aperture diaphragm completely opened. For better contrast with such large object fields, the
luminous-field aperture can be used by reducing its aperture by a certain amount. Overclosing
should be avoided so as not to impair the uniformity of the illumination of the field of view.
04/2013
OPERATION
Lighting and contrasting method in transmitted light
2/3 ... 4/5 of the
430037-7144-001
Fig. 4-1 Microscope settings in transmitted
light brightfield
Carl Zeiss
73