National DC5-163 Instructions Manual page 5

Compound biological microscope with digital camera
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C. Flip power switch located on back of microscope base "ON". Note that LED indicator will not light until USB
cable is connected to computer, when instructed in separate Quick Start Guide located on your CD.
D. Rotate intensity dial on illuminator base until image is illuminated.
E. Adjust intensity of light to match requirements of objective and specimen slide.
F. In case of equipment malfunction, see "Trouble Shooting" procedures.
2. Adjustment of viewing head:
A. The three-position sliding rod allows user to easily direct microscope image into desired path.
a) Rod pushed completely into head – 100% of microscope image is directed into binocular eyepieces.
b) Rod at mid-position (pull or push rod until you feel a gentle click stop) – 100% of microscope image is
directed to built-in camera.
c) Rod pulled to fully extended position – 30% of image is directed to binocular eyepieces and 70% directed
to built-in camera.
B. Position sliding rod completely into head to operate microscope.
C. Interpupillary adjustment of viewing head
a) Look through microscope and adjust distance between the two eyepiece tubes by grasping the sliding
mounts to left and right of eyepieces and sliding together or apart.
b) When a full field of view is observed through both tubes, and images blend into one, interpupillary distance
is corrected for your eyes. Check the interpupillary scale and note index reading for future reference, in
case other users will be changing this adjustment from time to time.
c) Adjust the diopter scales, located on each eyepiece tube, to the same numerical value as indicated on the
interpupillary scale. This must be done in order to maintain parfocality of objective lenses. If interpupillary
distance is changed, adjust eyepiece diopters accordingly.
3. Focusing the microscope.
A. Position the 4x objective lens into the optical path, making sure that lens is properly indexed in its click-stop
position.
B. Place standard specimen slide (cover slip up) on top of stage surface.
a) Swing moveable finger on slide holder outward. Place specimen slide against fixed side of slide holder.
Slowly release moveable finger until it makes contact with specimen slide.
C. Rotate coarse focusing controls until specimen comes into focus.
D. Adjust fine focus controls until specimen is in sharp focus.
E. Adjust diopter for difference in eyesight.
a) Using right eye, peer into the right eyepiece tube. Adjust sharpness of image by utilizing fine focus
controls.
b) Using left eye, peer into the left eyepiece tube. Adjust sharpness of image by turning diopter adjustment
located on left eyepiece tube.
F. Adjusting the aperture (opening) of iris diaphragm.
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