Nikon eclipse E200 Instructions Manual page 80

Causes
Condenser position too low.
Aperture diaphragm closed too far.
Dirt or dust on the lens (condenser,
objective, field lens, eyepiece,
specimen).
Poor image quality (low resolution, contrast too low or too high)
Causes
Condenser position too low.
Cover glass too thick or thin.
Slide upside down.
No cover glass attached to the slide.
No immersion oil used on the front lens
of the oil-immersion objective.
Nikon immersion oil is not used for
oil-immersion observation.
Air bubbles in immersion oil.
Chapter 8 Troubleshooting Tables
1 Optical
Dirt or dust in the viewfield.
78
Corrective Measures
If the microscope is equipped with a
field diaphragm, correct positioning so
that the field diaphragm image is
focused on the specimen surface. (P.57)
If the microscope is not equipped with a
field diaphragm, position the condenser
slightly lower than the upper limit.
Open properly. (P.59)
Clean the lens. (P.84)
Corrective Measures
If the microscope is equipped with a
field diaphragm, correct positioning so
that the fie ld diaphragm image is
focused on the specimen surface. (P.57)
If the microscope is not equipped with a
field diaphragm, position the condenser
slightly lower than the upper limit.
Use a cover glass of the specified
thickness (0.17 mm).
Turn over the slide so that the cover
glass faces up.
Attach a cover glass 0.17 mm thick.
Apply Nikon immersion oil to the
objective. (P.62)
Use Nikon immersion oil. (P.62)
Remove bubbles. (P.62)